Epithelial migration on the canine tympanic membrane     

Tabacca NE  Cole LK  Hillier A  Rajala-Schultz PJ 《Veterinary dermatology》2011,22(6):502-510

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Tumor slices as a model to evaluate doxorubicin in vitro treatment and expression of trios of genes PRSS11, MTSS1, CLPTM1 and PRSS11, MTSS1, SMYD2 in canine mammary gland cancer     

Renata A Sobral  Suzana T Honda  Maria Lucia H Katayama  Helena Brentani  M Mitzi Brentani  Diogo FC Patr?o  Maria Aparecida AK Folgueira 《Acta veterinaria Scandinavica》2008,50(1):27

Background

In women with breast cancer submitted to neoadjuvant chemotherapy based in doxorubicin, tumor expression of groups of three genes (PRSS11, MTSS1, CLPTM1 and PRSS11, MTSS1, SMYD2) have classified them as responsive or resistant. We have investigated whether expression of these trios of genes could predict mammary carcinoma response in dogs and whether tumor slices, which maintain epithelial-mesenchymal interactions, could be used to evaluate drug response in vitro.

Methods

Tumors from 38 dogs were sliced and cultured with or without doxorubicin 1 μM for 24 h. Tumor cells were counted by two observers to establish a percentage variation in cell number, between slices. Based on these results, a reduction in cell number between treated and control samples ≥ 21.7%, arbitrarily classified samples, as drug responsive. Tumor expression of PRSS11, MTSS1, CLPTM1 and SMYD2, was evaluated by real time PCR. Relative expression results were then transformed to their natural logarithm values, which were spatially disposed according to the expression of trios of genes, comprising PRSS11, MTSS1, CLPTM1 and PRSS11, MTSS1, SMYD2. Fisher linear discrimination test was used to generate a separation plane between responsive and non-responsive tumors.

Results

Culture of tumor slices for 24 h was feasible. Nine samples were considered responsive and 29 non-responsive to doxorubicin, considering the pre-established cut-off value of cell number reduction ≥ 21.7%, between doxorubicin treated and control samples. Relative gene expression was evaluated and tumor samples were then spatially distributed according to the expression of the trios of genes: PRSS11, MTSS1, CLPTM1 and PRSS11, MTSS1, SMYD2. A separation plane was generated. However, no clear separation between responsive and non-responsive samples could be observed.

Conclusion

Three-dimensional distribution of samples according to the expression of the trios of genes PRSS11, MTSS1, CLPTM1 and PRSS11, MTSS1, SMYD2 could not predict doxorubicin in vitro responsiveness. Short term culture of mammary gland cancer slices may be an interesting model to evaluate chemotherapy activity.  相似文献   

Acid‐extractable potassium in agricultural soils: Source minerals assessed by differential and quantitative X‐ray diffraction     

Ylva Andrist‐Rangel  Magnus Simonsson  Ingrid Öborn  Stephen Hillier 《植物养料与土壤学杂志》2013,176(3):407-419

Different extraction methods are used world‐wide in routine soil analysis to estimate long‐term potassium (K) reserves for plants. In Sweden, K extracted with 2 M HCl at 100°C (K_HCl) is frequently used, although with limited understanding of the phases extracted. In the present study, we quantified the effects of this extraction on soil minerals in particle size fractions ranging from clay to sand, and estimated their relative contribution to K_HCl. The study included three Swedish long‐term agricultural field experiments with texture ranging from loamy sand to silty clay, as well as mineral specimens of K feldspar. Total weight loss of particle size fractions was determined, and quantitative and differential X‐ray powder diffraction (QXRD, DXRD), applied on solids before and after extraction, was used to quantify the dissolution of individual mineral phases. QXRD and DXRD included spray‐drying of samples, addition of an internal standard and full pattern fitting, where a combination of mineral‐standard XRD traces was matched with the experimental one. Our results show that K_HCl was primarily associated with clay minerals concentrated in the two finest fractions (2–20 and < 2 μm). Highly expandable and mixed‐layer phyllosilicates were quantitatively the most important minerals dissolved. The K was released from micaceous layers in mixed‐layer phyllosilicates with a vermiculitic character. Whether di‐ or trioctahedral, a shared property of the dissolved phases was that they were rich in Fe. In the loamy sand, the coarser fractions (20–2000 μm), where feldspars were prominent, accounted for 35% of K_HCl. According to DXRD, there was no significant decrease in K feldspars in any of the samples, and K_HCl data for the feldspar specimens suggest that clay minerals contributed at least 70% of K_HCl also in the loamy sand. Our study provides insights about the soil minerals that contribute to the long‐term K delivery capacity of soils and an explanation for the prior observation that K_HCl is a dynamic fraction that can be affected by management.  相似文献   

Mineralogical budgeting of potassium in soil: A basis for understanding standard measures of reserve potassium     

Ylva Andrist‐Rangel  Magnus Simonsson  Stefan Andersson  Ingrid born  Stephen Hillier 《植物养料与土壤学杂志》2006,169(5):605-615

The study was conducted to investigate the relationship between some standard measures of soil reserve potassium (K) and soil mineralogy. Eight different agricultural soils from the N temperate and S boreal regions were studied and analyzed both by standard methods (exchangeable K, 2 M HCl‐ and aqua regia–extractable K) and by quantitative mineralogical methods based on X‐ray powder diffraction analysis of spray‐dried bulk soils. Linear regression and multivariate methods were used to assess the relationships between standard measures of soil reserve K and a number of soil chemical, physical, and mineralogical properties. A mineralogical budgeting approach, to estimate total K and its speciation between different mineral phases, is shown to be accurate after validation against total K analyzed geochemically. This approach enabled us to determine that both HCl‐ and aqua regia–extractable K were highly correlated with K in dioctahedral phyllosilicates and extracted 1%–17% and 5%–45% of total K, respectively. Neither extraction showed any obvious relationship to K in feldspar, which is frequently a larger reservoir of K in the soils examined.  相似文献   

The interaction between soil organic matter and soil clay minerals by selective removal and controlled addition of organic matter     

M. V. Cheshire  C. Dumat  A. R. Fraser  S. Hillier  S. Staunton 《European Journal of Soil Science》2000,51(3):497-509

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Results of intradermal tests in horses without atopy and horses with atopic dermatitis or recurrent urticaria.     

G Lorch  A Hillier  K W Kwochka  W A Saville  B E LeRoy 《American journal of veterinary research》2001,62(7):1051-1059

OBJECTIVE: To compare results of intradermal tests (IDT) for environmental allergens at 30 minutes and 4, 6, and 24 hours after injection in horses without atopy and horses with atopic dermatitis (AD) or recurrent urticaria (RU). ANIMALS: 22 horses without atopy, 10 horses with RU, and 7 horses with AD. PROCEDURE: In all horses, medical history was obtained, and results of physical examination, hematologic examination, serum biochemical analyses, examination of bronchoalveolar lavage fluid, and IDT with 73 allergens were examined. RESULTS: Horses with AD or RU had a significantly greater mean number of positive reactions for IDT, compared with horses without atopy. Horses with AD had a significantly greater number of positive reactions than horses without atopy in every allergen group at all time periods, except for molds at 4 and 24 hours. Horses with RU had a significantly greater number of positive reactions than horses without atopy in every allergen group, except for molds at 30 minutes and 4 and 6 hours, trees at 4 and 6 hours, and grasses at 4 hours. CONCLUSIONS AND CLINICAL RELEVANCE: A significantly greater number of positive reactions for IDT in horses with AD or RU, compared with horses without atopy, provides evidence of type-I IgE-mediated hypersensitivity for these diseases. Evaluation of results of IDT performed in horses with AD or RU is useful in determining specific allergens for the formulation of immunotherapy along with providing identification of allergens that could be useful when creating avoidance strategies.  相似文献   

Endothelin mediated contraction of equine laminar veins     

Keen JA  Hillier C  McGorum BC  Nally JE 《Equine veterinary journal》2008,40(5):488-492

REASONS FOR PERFORMING STUDY: Endothelin-1 (ET-1) may be a key mediator in the pathogenesis of laminitis, but endothelin-mediated responses in the venous microcirculation of the equine foot have yet to be fully characterised. OBJECTIVES: To characterise the response of equine laminar veins to ET-1 and evaluate the ET-1 receptor subtypes that mediate this response. METHODS: Small veins (150-500 microns) draining the equine digital laminae from healthy horses and ponies subjected to euthanasia at an abattoir were investigated using wire myography. Concentration response curves were constructed for ET-1 in the presence of ETA (BQ123) and ETB (BQ788) receptor antagonists, and L-NAME, a nitric oxide synthase blocker. The selective ETB receptor agonist BQ3020 was investigated alone and following incubation with L-NAME, with or without BQ788. RESULTS: Endothelin-1 contraction of laminar veins was significantly inhibited by BQ123 but not by BQ788. In the presence of L-NAME, sensitivity of laminar veins to ET-1 was enhanced 4-fold, and further addition of BQ788 did not alter this increased sensitivity. BQ3020 induced no venoconstriction; however, in the presence of L-NAME, it caused contraction of veins with approximately 30% of the efficacy of ET-1. The action of BQ3020 in the presence of L-NAME was abolished by BQ788. CONCLUSIONS: Both ETA and ETB receptors are involved in the net tonic response to ET-1 in normal laminar veins. A population of ETB receptors may be present on the vascular endothelium and on smooth muscle of laminar veins, and the action of ET-1 at these 2 sites is likely to be approximately equal and opposite. POTENTIAL RELEVANCE: Our results clarify the function of the ET-1 receptor subtypes in laminar veins from healthy horses. Further study of ET-1 receptors in laminitic horses is therefore warranted.  相似文献   

Cool Farm Tool – Potato: Model Description and Performance of Four Production Systems     

A. J. Haverkort  J. G. Hillier 《Potato Research》2011,54(4):355-369

The Cool Farm Tool – Potato (CFT-Potato) is a spreadsheet programme that allows the calculation of the amount of CO₂ equivalents that it costs to produce 1 t of potato. The spreadsheet was adapted from an original generic version of the tool, and completed for potato production in diverse production areas in the world applying different levels of technology. The CO₂ embedded in chemicals during their production and released from the soil after nitrogen fertilization in the CFT-Potato has been updated to consider more recent products and production methods. Energy costs of the operations in the original version taken from generic data provided by the American Society of Agricultural and Biological Engineers Standard, however, were altered (usually increased) where there was evidence from practical sources that the original figures did not apply. For example, the figure of around 16 l of diesel per ha for potato harvesting in the original version was corrected to 60 l of diesel per ha based on observational data. Figures for typical potato operations such as windrowing were supplied. Irrigation with pumps powered by diesel or electricity from the grid, with a centre pivot, a rain gun, drip irrigation and flooding and energy cost for extracting water from deeper sources were also added. We added data for grading, washing, store loading and unloading, the application of a sprout suppressant and storage with ventilation of ambient air or forced refrigeration. The CFT-Potato can be used by growers to calculate the actual costs of 1 t of potato in terms of kilograms CO₂ and explore the repercussion of altered management options. Here the comparison of four potato production systems in the Netherlands is shown: seed potatoes (115 kg CO₂/t), table potatoes (77 kg CO₂/t), starch potatoes (71 kg CO₂/t) and organic potato (82 kg CO₂/t). Based on potato dry matter, however, starch potato has the lowest footprint mainly due to the extensive use of pig slurry of which the production and transport CO₂ costs are attributed to the pig production chain.  相似文献   

Reply     

MP WARD  M. FLANAGAN  FC BALDOCK  TE CARPENTER 《Australian veterinary journal》1995,72(9):359-360

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Quantitation of house dust mites and house dust mite allergens in the microenvironment of dogs     

Randall A  Hillier A  Cole LK  Kwochka KW  Needham G  Wassom DL 《American journal of veterinary research》2003,64(12):1580-1588

OBJECTIVE: To quantitate the density of Dermatophagoides farinae and D pteronyssinus and concentrations of house dust mite (HDM) allergens (Der f 1, Der p 1, and Group 2 allergens) in the indoor microenvironment of dogs. SAMPLE POPULATION: 50 homes in Columbus, Ohio. PROCEDURES: In each home, samples of dust were collected from 3 locations in which dogs spent most time. Whenever possible, the species of mites collected was identified. Mite density (mites/g of dust) was assessed, and allergen concentrations were assayed by standardized ELISAs. Relative humidity and temperature in each home were monitored during a 5-day period. Characteristics of homes and sample sources were evaluated. RESULTS: Dust samples from all 50 homes contained > or = 1 HDM allergen; Der f 1 and Der p 1 were detected in 100 and 74% of homes, respectively. Fifteen homes had HDMs; compared with D pteronyssinus, D farinae was found more commonly (14/15 homes) and at a higher density. Basements, homes without central air-conditioning, and dog beds that were > or = 1 year old had high HDM allergen concentrations. Homes with > or = 2 microg of Der f 1 or Group 2 allergens/g of dust or > or = 100 mites/g of dust were significantly more likely to have a maximum relative humidity > or = 75%. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated the presence of HDMs and HDM allergens in the specific microenvironment of dogs in homes. Factors associated with high levels of exposure were identified, which may be associated with increased risk for sensitization and development of atopic diseases.  相似文献