Stability and emulsion-forming ability of water-soluble fish myofibrillar protein prepared by conjugation with alginate oligosaccharide     

Sato R  Katayama S  Sawabe T  Saeki H 《Journal of agricultural and food chemistry》2003,51(15):4376-4381

Carp myofibrillar protein (Mf) was conjugated with alginate oligosaccharide (AO) through the Maillard reaction under low relative humidity, and the functional properties of the Mf-AO conjugate were investigated under different NaCl concentrations and pH levels. Mf became highly solubilized at lower NaCl concentrations by conjugation with AO, with a slight loss of available lysine. The thermal stability of Mf was effectively improved by conjugation with AO. Heat treatment at 80 degrees C for 2 h had no effect on the solubility of the Mf-AO conjugate attached to 227 microg/mg of AO regardless of the NaCl concentration and pH. Furthermore, the Mf-AO conjugate showed excellent emulsion-forming ability regardless of NaCl concentration. The improved functionalities of Mf by conjugation with AO remained even at a nearly isoelectric point. These results indicate that conjugation with AO through the Maillard reaction is an effective way to prepare high-functional food material from fish muscle protein.  相似文献   

Evaluation of biological activities of extracts from the fruiting body of <Emphasis Type="Italic">Pleurotus citrinopileatus</Emphasis> for skin cosmetics     

Tian-Xiao Meng  Shoko Furuta  Sayaka Fukamizu  Ryoko Yamamoto  Hiroya Ishikawa  Enos Tangke Arung  Kuniyoshi Shimizu  Shoji Ohga  Ryuichiro Kondo 《Journal of Wood Science》2011,57(5):452-458

Pleurotus citrinopileatus Singer has recently become a popular delicacy in East Asian countries. We prepared a methanol extract, soluble fractions from the methanol extract, and a hot water extract of the fruiting bodies of P. citrinopileatus. The biological activities such as melanin biosynthesis inhibition, tyrosinase inhibition, antioxidant activities [1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, oxygen radical absorbance capacity (ORAC), and superoxide dismutase (SOD)-like activity], antibacterial activities, and antihyaluronidase activities of these extracts were evaluated. We found that the n-hexanesoluble, diethyl ether-soluble, and ethyl acetate-soluble fractions exhibited melanin biosynthesis inhibition in B16 melanoma cells, as well as antioxidant (ORAC) and antibacterial activities. However, the n-butanol-soluble and water-soluble fractions and the methanol and hot water extracts exhibited antioxidant (DPPH radical scavenging, SOD-like activity) and antihyaluronidase activities. These results indicate that the fruiting bodies of P. citrinopileatus have the potential to be used as an ingredient in skin cosmetics.  相似文献   

Rapid, Micro-Methods to Estimate Plant Silicon Content by Dilute Hydrofluoric Acid Extraction and Spectrometric Molybdenum Method     

Kimio Saito  Akiko Yamamoto  Tongmin Sa  Masahiko Saigusa 《Soil Science and Plant Nutrition》2005,51(1):29-36

By treating 0.5 g DW of a plant sample directly with 10 ml of a dilute hydrofluoric acid solution (HF solution, 1.5 M HF—0.6 M HCl), all the silica in plant (as much as 150 mg SiO₂) was dissolved within 1 h. After dilution of the extract with 40 mL of distilled water, the silica in the extract was measured by the spectrometric molybdenum yellow method. The molybdenum yellow method, in which silica in 0.1 mL of the diluted extract can be determined in 8 min, is well suited to rapid, micro-estimations of the silica content in plants. In the micro-modification, the size of the plant sample was reduced to 100 mg DW. The analytical procedure was simple, and the analytical time was less than 2 h. The method can save much labor and time, compared with the gravimetric analysis. The dissolution with HF solution and the molybdenum yellow method were also applied to the measurement of the content of silica separated by acid digestion of rice plants. Excellent agreement in the silica measurement of rice plants was confirmed among the direct extraction method, the gravimetric method and the digestion-separation-dissolution method. In the molybdenum yellow method, the addition of boric acid enabled to mask the interference of hydrofluoric acid, and the least amount of citric acid required for the elimination of phosphorus interference was proposed. In conclusion in this report, recommended methods for the rapid estimation of the silica content in rice plants were presented.  相似文献   

暴雨径流期间前次洪水对悬移质传输影响的研究     

OkazawaHiromu  NagasawaTetuaki 《中国水土保持》2002,(7):17-17

对日本北海道西南部的两个不同土地利用方式流域的几场降雨中的悬移质输沙量进行了观测。一个流域完全被森林所覆盖 ,另一个流域农业用地占 35%。这个地区主要种植日耕型的高原作物 ,该作物系喂养菜牛的饲料。从 1 997年 5月到 1 998年 1 0月 (不包括 1 997年 1 1月至1 998年 4  相似文献   

Cycloamylose (cyclodextrin) glucanotransferase degrades intact granules of potato raw starch     

Yamamoto K  Zhang ZZ  Kobayashi S 《Journal of agricultural and food chemistry》2000,48(3):962-966

Cycloamylose (cyclodextrin) glucanotransferase (EC 2.4.1.19, CGTase) originated from Bacillus macerans degraded intact granules of potato raw starch and converted them into cyclodextrins (CDs). The degradation required sufficient stirring of starch-CGTase suspension. The morphology of the degraded starch granules was unique; that is, the inner part of the granules was observed by scanning electron microscope to be more susceptible to CGTase than the outer part. Effects of pH, temperature, starch concentration, and enzyme amount on CD production were studied.  相似文献   

Reverse phase liquid chromatographic determination and confirmation of aflatoxin M1 in cheese     

K Hisada  H Terada  K Yamamoto  H Tsubouchi  Y Sakabe 《Journal of the Association of Official Analytical Chemists》1984,67(3):601-606

A systematic method is proposed for determination and confirmation of aflatoxin M1 in cheese by liquid chromatography (LC). A sample of cheese is extracted with chloroform, cleaned up on 2 silica gel columns followed by a Sep-Pak C18 cartridge, and chromatographed on a 5 microns octadecyl silica column with fluorometric detection. The sample extract or standard is treated with n-hexane-trifluoroacetic acid (TFA) (4 + 1) for 30 min at 40 degrees C. Analysis by LC with TFA-treatment of the extract provides quantitative data. Multiple assays of 5 samples of Gouda cheese spiked with aflatoxin M1 at levels of 0.5, 0.1, and 0.05 ng/g showed average recoveries of 93.2, 91.6, and 92.4%, with coefficients of variation of 2.63, 3.97, and 4.52%, respectively. Assay of 5 naturally contaminated cheeses resulted in 0.051-0.448 ng/g of aflatoxin M1. Limit of quantitation is about 0.01 ng/g. The identity of aflatoxin M1 is confirmed by treating aflatoxin M1 or the M2a derivative with TFA-methanol (or ethanol) (3 + 1). The TFA-methanol reaction products of M2a could be detected quantitatively.  相似文献   

Central role for the lens in cave fish eye degeneration     

Yamamoto Y  Jeffery WR 《Science (New York, N.Y.)》2000,289(5479):631-633

Astyanax mexicanus is a teleost with eyed surface-dwelling and eyeless cave-dwelling forms. Eye formation is initiated in cave fish embryos, but the eye subsequently arrests and degenerates. The surface fish lens stimulates growth and development after transplantation into the cave fish optic cup, restoring optic tissues lost during cave fish evolution. Conversely, eye growth and development are retarded following transplantation of a surface fish lens into a cave fish optic cup or lens extirpation. These results show that evolutionary changes in an inductive signal from the lens are involved in cave fish eye degeneration.  相似文献   

Monitoring biosynthesis of wheat cell-wall phenylpropanoids in situ     

Lewis NG  Yamamoto E  Wooten JB  Just G  Ohashi H  Towers GH 《Science (New York, N.Y.)》1987,237(4820):1344-1346

Lignins and suberins are complex plant cell-wall macromolecules that are composed mainly of phenylpropanoid residues derived from L-phenylalanine. Lignins and suberins are considered to be covalently linked to carbohydrates and to lipids, respectively. The bonding of these important structural materials within cell walls has never been established. By feeding specifically labeled [(13)C] ferulic acid over extended durations to seedlings of Triticum aestivum L. and by using solid-state carbon-13 nuclear magnetic resonance techniques, the major resonances due to specific carbons in the propanoid side chains of these cell-wall polymers have been identified in situ. The signals were found to differ significantly from those of synthetic lignins, which have usually been considered to be good approximations of natural lignin structure.  相似文献   

Glucocorticoid receptor mutants that define a small region sufficient for enhancer activation   总被引：38，自引：0，他引：38  

R Miesfeld  P J Godowski  B A Maler  K R Yamamoto 《Science (New York, N.Y.)》1987,236(4800):423-427

  相似文献   

Infection of HTLV-III/LAV in HTLV-I-carrying cells MT-2 and MT-4 and application in a plaque assay   总被引：120，自引：0，他引：120  

S Harada  Y Koyanagi  N Yamamoto 《Science (New York, N.Y.)》1985,229(4713):563-566

The human T-cell lines MT-2 and MT-4 carry the human T-cell leukemia virus type I (HTLV-I). When MT-2 and MT-4 were infected with HTLV-III, the probable etiologic agent of the acquired immune deficiency syndrome (AIDS), rapid cytopathogenic effects and cytotoxicity were observed that made it possible to titrate the biologically active virus in a plaque-forming assay. The cytopathogenic effects were preceded by the rapid induction and increase of HTLV-III antigens as revealed by immunofluorescence and immunoprecipitation. Activities of HTLV-III were neutralized by the human antibodies against the virus when immunofluorescence and plaque assays were used. Essentially the same results were obtained with the lymphadenopathy-associated virus (LAV1).  相似文献