Seizure discharges evoked in vitro in thin section from guinea pig hippocampus   总被引：3，自引：0，他引：3  

C Yamamoto  N Kawai 《Science (New York, N.Y.)》1967,155(760):341-342

A thin section prepared from guinea pig hippocampus produced, in chloride-free medium, a train of seizure discharges in response to a single shock applied to the section. Generation of these discharges was ascribed to the lack of inhibitory processes in an absence of chloride ion.  相似文献   

Induction of inflammatory arthropathy resembling rheumatoid arthritis in mice transgenic for HTLV-I   总被引：28，自引：0，他引：28  

Y Iwakura  M Tosu  E Yoshida  M Takiguchi  K Sato  I Kitajima  K Nishioka  K Yamamoto  T Takeda  M Hatanaka 《Science (New York, N.Y.)》1991,253(5023):1026-1028

Human T cell leukemia virus type-I (HTLV-I) is the etiologic agent of adult T cell leukemia and has also been suggested to be involved in other diseases such as chronic arthritis or myelopathy. To elucidate pathological roles of the virus in disease, transgenic mice were produced that carry the HTLV-I genome. At 2 to 3 months of age, many of the mice developed chronic arthritis resembling rheumatoid arthritis. Synovial and periarticular inflammation with articular erosion caused by invasion of granulation tissues were marked. These observations suggest a possibility that HTLV-I is one of the etiologic agents of chronic arthritis in humans.  相似文献   

Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus     

Watanabe T  Tomizawa S  Mitsuya K  Totoki Y  Yamamoto Y  Kuramochi-Miyagawa S  Iida N  Hoki Y  Murphy PJ  Toyoda A  Gotoh K  Hiura H  Arima T  Fujiyama A  Sado T  Shibata T  Nakano T  Lin H  Ichiyanagi K  Soloway PD  Sasaki H 《Science (New York, N.Y.)》2011,332(6031):848-852

Genomic imprinting causes parental origin-specific monoallelic gene expression through differential DNA methylation established in the parental germ line. However, the mechanisms underlying how specific sequences are selectively methylated are not fully understood. We have found that the components of the PIWI-interacting RNA (piRNA) pathway are required for de novo methylation of the differentially methylated region (DMR) of the imprinted mouse Rasgrf1 locus, but not other paternally imprinted loci. A retrotransposon sequence within a noncoding RNA spanning the DMR was targeted by piRNAs generated from a different locus. A direct repeat in the DMR, which is required for the methylation and imprinting of Rasgrf1, served as a promoter for this RNA. We propose a model in which piRNAs and a target RNA direct the sequence-specific methylation of Rasgrf1.  相似文献   

Mutations in dynein link motor neuron degeneration to defects in retrograde transport     

Hafezparast M  Klocke R  Ruhrberg C  Marquardt A  Ahmad-Annuar A  Bowen S  Lalli G  Witherden AS  Hummerich H  Nicholson S  Morgan PJ  Oozageer R  Priestley JV  Averill S  King VR  Ball S  Peters J  Toda T  Yamamoto A  Hiraoka Y  Augustin M  Korthaus D  Wattler S  Wabnitz P  Dickneite C  Lampel S  Boehme F  Peraus G  Popp A  Rudelius M  Schlegel J  Fuchs H  Hrabe de Angelis M  Schiavo G  Shima DT  Russ AP  Stumm G  Martin JE  Fisher EM 《Science (New York, N.Y.)》2003,300(5620):808-812

Degenerative disorders of motor neurons include a range of progressive fatal diseases such as amyotrophic lateral sclerosis (ALS), spinal-bulbar muscular atrophy (SBMA), and spinal muscular atrophy (SMA). Although the causative genetic alterations are known for some cases, the molecular basis of many SMA and SBMA-like syndromes and most ALS cases is unknown. Here we show that missense point mutations in the cytoplasmic dynein heavy chain result in progressive motor neuron degeneration in heterozygous mice, and in homozygotes this is accompanied by the formation of Lewy-like inclusion bodies, thus resembling key features of human pathology. These mutations exclusively perturb neuron-specific functions of dynein.  相似文献   

Full three-dimensional photonic bandgap crystals at near-infrared wavelengths     

Noda S  Tomoda K  Yamamoto N  Chutinan A 《Science (New York, N.Y.)》2000,289(5479):604-606

An artificial crystal structure has been fabricated exhibiting a full three-dimensional photonic bandgap effect at optical communication wavelengths. The photonic crystal was constructed by stacking 0.7-micrometer period semiconductor stripes with the accuracy of 30 nanometers by advanced wafer-fusion technique. A bandgap effect of more than 40 decibels (which corresponds to 99.99% reflection) was successfully achieved. The result encourages us to create an ultra-small optical integrated circuit including a three-dimensional photonic crystal waveguide with a sharp bend.  相似文献   

In vitro transcription enhancement by purified derivatives of the glucocorticoid receptor   总被引：15，自引：0，他引：15  

L P Freedman  S K Yoshinaga  J N Vanderbilt  K R Yamamoto 《Science (New York, N.Y.)》1989,245(4915):298-301

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Transcription factor interactions: selectors of positive or negative regulation from a single DNA element   总被引：137，自引：0，他引：137  

M I Diamond  J N Miner  S K Yoshinaga  K R Yamamoto 《Science (New York, N.Y.)》1990,249(4974):1266-1272

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Statement on scientific publication and security     

Atlas R  Campbell P  Cozzarelli NR  Curfman G  Enquist L  Fink G  Flanagin A  Fletcher J  George E  Hammes G  Heyman D  Inglesby T  Kaplan S  Kennedy D  Krug J  Levinson R  Marcus E  Metzger H  Morse SS  O'Brien A  Onderdonk A  Poste G  Renault B  Rich R  Rosengard A  Salzburg S  Scanlan M  Shenk T  Tabor H  Varmus H  Wimmer E  Yamamoto K;Journal Editors  Authors Group 《Science (New York, N.Y.)》2003,299(5610):1149

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Cytokinin oxidase regulates rice grain production   总被引：25，自引：0，他引：25  

Ashikari M  Sakakibara H  Lin S  Yamamoto T  Takashi T  Nishimura A  Angeles ER  Qian Q  Kitano H  Matsuoka M 《Science (New York, N.Y.)》2005,309(5735):741-745

Most agriculturally important traits are regulated by genes known as quantitative trait loci (QTLs) derived from natural allelic variations. We here show that a QTL that increases grain productivity in rice, Gn1a, is a gene for cytokinin oxidase/dehydrogenase (OsCKX2), an enzyme that degrades the phytohormone cytokinin. Reduced expression of OsCKX2 causes cytokinin accumulation in inflorescence meristems and increases the number of reproductive organs, resulting in enhanced grain yield. QTL pyramiding to combine loci for grain number and plant height in the same genetic background generated lines exhibiting both beneficial traits. These results provide a strategy for tailormade crop improvement.  相似文献   

Signal transduction and transcriptional regulation by glucocorticoid receptor-LexA fusion proteins   总被引：35，自引：0，他引：35  

P J Godowski  D Picard  K R Yamamoto 《Science (New York, N.Y.)》1988,241(4867):812-816

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