Isolation and serial propagation of porcine epidemic diarrhea virus in cell cultures and partial characterization of the isolate.   总被引：3，自引：0，他引：3  

K Kusanagi  H Kuwahara  T Katoh  T Nunoya  Y Ishikawa  T Samejima  M Tajima 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(2):313-318

Porcine epidemic diarrhea virus (PEDV) was isolated in Vero cell cultures from the small intestine of a piglet experimentally infected with porcine coronavirus 83P-5, that had been isolated during outbreaks of porcine acute diarrhea and passaged in piglets. The isolation of the PEDV was successful only in Vero cells maintained in the maintenance medium (MM) containing trypsin. Infected Vero cell cultures exhibited CPE characterized by cell-fusion and syncytial formation, as well as cytoplasmic fluorescence when examined by the indirect immunofluorescent test using rabbit anti-83P-5 virus serum. The isolate was adapted to serial propagation in Vero cell cultures by adding trypsin to MM. Vero cell-adapted PEDV was successfully propagated in the MA104, CPK and ESK cell lines in the presence of trypsin in MM. Vero cell-adapted PEDV had morphologic and physicochemical characteristics similar to those of other members of the coronaviridae. The isolate differed serologically from porcine transmissible gastroenteritis (TGE) and porcine hemagglutinating encephalomyelitis viruses, and no antigenic relationship between the isolate and TGE virus could be detected by the indirect immunofluorescent test. Attempts to isolate PEDV in 6 types of primary fetal pig cell cultures and 6 of 10 established cell lines resulted in the failure, probably because these cells were damaged by the action of trypsin.  相似文献   

Clinical and molecular analysis of GM2 gangliosidosis in two apparent littermate kittens of the Japanese domestic cat     

Hasegawa D  Yamato O  Kobayashi M  Fujita M  Nakamura S  Takahashi K  Satoh H  Shoda T  Hayashi D  Yamasaki M  Maede Y  Arai T  Orima H 《Journal of Feline Medicine and Surgery》2007,9(3):232-237

This case report documents clinical and molecular findings in two littermate kittens of the Japanese domestic cat with GM2 gangliosidosis variant 0. Analysis included detailed physical, magnetic resonance imaging, biochemical, pathological and genetic examinations. At first, these littermate kittens showed typical cerebellar signs at approximately 2 months of age. About 2 months later, they progressively showed other neurological signs and subsequently died at about 7 months of age. Magnetic resonance imaging just before the death showed an enlarged ventricular system, T1 hyperintensity in the internal capsule, and T2 hyperintensity in the white matter of the whole brain. Histological findings suggested a type of lysosomal storage disease. Biochemical studies demonstrated that the kittens were affected with GM2 gangliosidosis variant 0, and a DNA assay finally demonstrated that these animals were homozygous for the mutation, which the authors had identified in a different family of the Japanese domestic cat. The findings in the present cases provide useful information about GM2 gangliosidosis variant 0 in Japanese domestic cats.  相似文献   

Surveillance of Avian Paramyxovirus in Migratory Waterfowls in the San-in Region of Western Japan from 2006 to 2012     

Dennis V. UMALI  Hiroshi ITO  Hiromitsu KATOH  Toshihiro ITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(3):423-430

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黑龙江省主栽水稻品种抽穗模型研究初报     

许显滨  矫江  卞景阳  谭贺  鲛岛良次  神田英司 《北方水稻》2007,(1):35-36,44

采用黑龙江省生产上主栽的早熟、中熟和晚熟的6个水稻品种,在哈尔滨地区做分期栽培田间试验。调查记载播种、插秧和抽穗期。采取平行观测的气象资料,分析温度对水稻抽穗期和水稻营养生长期的影响。结果表明:不同的插秧期水稻抽穗期变化很大,不同熟期的水稻品种插秧至抽穗期需要的有效积温差别明显,但每个品种需要的有效积温很稳定。  相似文献