Induction of apoptosis by the oolong tea polyphenol theasinensin A through cytochrome c release and activation of caspase-9 and caspase-3 in human U937 cells   总被引：5，自引：0，他引：5  

Pan MH  Liang YC  Lin-Shiau SY  Zhu NQ  Ho CT  Lin JK 《Journal of agricultural and food chemistry》2000,48(12):6337-6346

This study examined the growth inhibitory effects of theasinensin A (from oolong tea) and black tea polyphenols, including theaflavin (TF-1), a mixture (TF-2) of theaflavin-3-gallate (TF-2a) and theaflavin-3'-gallate (TF-2b), and theaflavin-3,3'-digallate (TF-3) in human cancer cells. Theasinensin A, TF-1, and TF-2 displayed strong growth inhibitory effects against human histolytic lymphoma U937, with estimated IC50 values of 12 microM, but were less effective against human acute T cell leukemia Jurkat, whereas TF-3 and (-)-epigallocatechin-3-gallate (EGCG) had lower activities. The molecular mechanisms of tea polyphenol-induced apoptosis as determined by annexin V apoptosis assay, DNA fragmentation, and caspase activation were further investigated. Loss of membrane potential and reactive oxygen species (ROS) generation were also detected by flow cytometry. Treatment with tea polyphenols caused rapid induction of caspase-3, but not caspase-1, activity and stimulated proteolytic cleavage of poly(ADP-ribose) polymerase (PARP). Pretreatment with a potent caspase-3 inhibitor, Z-Asp-Glu-Val-Asp-fluoromethyl ketone, inhibited theasinensin A induced DNA fragmentation. Furthermore, it was found that theasinensin A induced loss of mitochondrial transmembrane potential, elevation of ROS production, release of mitochondrial cytochrome c into the cytosol, and subsequent induction of caspase-9 activity. These results indicate that theasinensin A allows caspase-activated deoxyribonuclease to enter the nucleus and degrade chromosomal DNA and induces DFF-45 (DNA fragmentation factor) degradation. The results suggest that induction of apoptosis by theasinensin A may provide a pivotal mechanism for their cancer chemopreventive function.  相似文献   

Identification of characteristic aroma-active compounds from water dropwort (Oenanthe javanica DC.)     

Seo WH  Baek HH 《Journal of agricultural and food chemistry》2005,53(17):6766-6770

Characteristic aroma components of water dropwort (Oenanthe javanica DC.) were evaluated by aroma extract dilution analysis and solid-phase microextraction-gas chromatography-olfactometry. Alpha-Terpinolene (plastic/cucumber-like) was the most intense aroma-active compound in water dropwort. Other potent aroma-active compounds included p-cymene (kerosene-like), alpha-terpinene (lemon), (E)-caryophyllene (woody), (Z,E)-alpha-farnesene (woody), hexanal (green), (Z)-3-hexenol (green), phenylacetaldehyde (honey), (E)-2-nonenal (cucumber), bornyl acetate (cooked vegetable), and gamma-terpinene (lemon). Of these, p-cymene was believed to be primarily responsible for the distinct kerosene-like aroma note of water dropwort. The aroma property of p-cymene was dependent on its concentration and was described as kerosene-like at relatively high concentrations but changed to citrus and green aroma notes at low concentrations.  相似文献   

Ecdysteroids of quinoa seeds (Chenopodium quinoa Willd.)     

Zhu N  Kikuzaki H  Vastano BC  Nakatani N  Karwe MV  Rosen RT  Ho CT 《Journal of agricultural and food chemistry》2001,49(5):2576-2578

Quinoa (Chenopodium quinoa) is a hardy and nutritious Latin American pseudo-cereal. Studies on the seeds led to the isolation of five ecdysteroids using column chromatography. Their structures were determined as ecdysterone, makisterone A, 24-epi-makisterone A, 24(28)-dehydromakisterone A, and 20,26-dihydroxyecdysone by spectroscopic methods. This study demonstrates that quinoa seeds are a source of ecdysteroids, which were reported to be molting hormones in insects.  相似文献   

Ballistic 2-d imaging through scattering walls using an ultrafast optical kerr gate     

Wang L  Ho PP  Liu C  Zhang G  Alfano RR 《Science (New York, N.Y.)》1991,253(5021):769-771

An ultrafast optical shutter was used to image ultrasmall objects hidden behind scattering walls by a procedure that selects in time the ballistic component and rejects the scattered diffusive light. Scattering walls used in this experiment included human breast tissue, chicken breast tissue, and a water suspension of polystyrene particles with scattering coefficients up to 21.7. Submillimeter resolution was achieved for two-dimensional ballistic images of a single point, a double-point fluorescence source, and a bar test chart in or behind these different turbid media.  相似文献   

Functional interaction and partial homology between human immunodeficiency virus and neuroleukin   总被引：8，自引：0，他引：8  

M R Lee  D D Ho  M E Gurney 《Science (New York, N.Y.)》1987,237(4818):1047-1051

Dementia is common in patients with AIDS, but the mechanism by which the human immunodeficiency virus type 1 (HIV-1) causes the neurological impairment is unknown. In this study the possibility that an antigen of HIV-1 suppresses neuronal responses to neurotrophic factors was examined. Both HIV-1 and a related retrovirus, simian immunodeficiency virus (SIV), inhibited the growth of sensory neurons from chick dorsal root ganglia in medium containing neuroleukin (NLK) but not in medium containing nerve growth factor. An unrelated type D retrovirus, simian acquired immunodeficiency syndrome virus, did not affect the growth of neurons in the presence of either neurotrophic factor. The inhibition by HIV-1 of neuron growth in the presence of NLK was found to be due to the gp120 envelope glycoprotein. Regions of sequence homology between gp120 and NLK may account for this inhibitory property of gp120 and functional interactions between gp120 and NLK may be important in the pathogenesis of the AIDS dementia complex.  相似文献   

Single-bond formation and characterization with a scanning tunneling microscope     

Lee HJ  Ho W 《Science (New York, N.Y.)》1999,286(5445):1719-1722

A scanning tunneling microscope (STM) was used to manipulate the bonding of a carbon monoxide (CO) molecule and to analyze the structure and vibrational properties of individual products. Individual iron (Fe) atoms were evaporated and coadsorbed with CO molecules on a silver (110) surface at 13 kelvin. A CO molecule was transferred from the surface to the STM tip and bonded with an Fe atom to form Fe(CO). A second CO molecule was similarly transferred and bonded with Fe(CO) to form Fe(CO)(2). Controlled bond formation and characterization at the single-bond level probe chemistry at the spatial limit.  相似文献   

"Tubules-Within-a-Tubule" Hierarchical Order of Mesoporous Molecular Sieves in MCM-41   总被引：1，自引：0，他引：1  

HP Lin  CY Mou 《Science (New York, N.Y.)》1996,273(5276):765-768

The recently discovered mesoporous aluminosilicate MCM-41 consists of hexagonal arrays of nanometer-sized cylindrical pores. It is shown that this material can be synthesized by cooperative condensation of silicate and cylindrical cationic micelles. Careful control of the surfactant-water content and the rate of condensation of silica at high alkalinity resulted in hollow tubules 0.3 to 3 micrometers in diameter. The wall of the tubules consisted of coaxial cylindrical pores, nanometers in size, that are characteristic of those of MCM-41. The formation of this higher order structure may take place through a liquid crystal phase transformation mechanism involving an anisotropic membrane-to-tubule phase change. The hierarchical organization of this "tubules-within-a-tubule" particle texture is similar to that of the frustules of marine diatoms.  相似文献   

Electrical resistance of long conjugated molecular wires     

Ho Choi S  Kim B  Frisbie CD 《Science (New York, N.Y.)》2008,320(5882):1482-1486

The charge transport mechanism of a wire can be revealed by how its electrical resistance varies with length. We have measured the resistance and current-voltage characteristics of conjugated molecular wires ranging in length from 1 to 7 nanometers, connected between metal electrodes. We observe the theoretically predicted change in direct-current transport from tunneling to hopping as a function of systematically controlled wire length. We also demonstrate that site-specific disruption of conjugation in the wires greatly increases resistance in the hopping regime but has only a small effect in the tunneling regime. These nanoscale transport measurements elucidate the role of molecular length and bond architecture on molecular conductivity and open opportunities for greater understanding of electrical transport in conjugated polymer films.  相似文献   

PHYSICS. Arrival of the fermion superfluid     

Ho TL 《Science (New York, N.Y.)》2004,305(5687):1114-1115

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Narcotic tolerance and dependence and serotonin turnover     

R J Hitzemann  I K Ho  H H Loh 《Science (New York, N.Y.)》1972,178(61):645-647

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