Electron microscopy analysis of carboxymethylcellulase in rhizobia     

Chun-Yi Hu  Wen-Sheng Tseng  Shu-Ling Hsieh  Yo-Chia Chen  Shang-Shyng Yang 《Soil biology & biochemistry》2009,41(2):431-434

Here we analyzed carboxymethylcellulase (CMCase; EC 3, 2, 1, 4), one of the key enzymes in the early symbiotic process, in Rhizobium. Specific immunogold labeling of electron microscopy was confirmed in Sinorhizobium fredii BCRC15769, ATCC35423, Sinorhizobium meliloti ATCC9930, and barely detected in Bradyrhizobium japonicum BCRC13528, ATCC10324 and Rhizobium rhizogenes ATCC11325. Non-specific labeling was detected in Rhizobium leguminosarum bv. viceae ATCC10004, Rhizobium leguminosarum bv. trifolii ATCC10328, and Mesorhizobium loti ATCC33669. Treatment of S. fredii BCRC15769 in the early log phase with the flavonoid genistein caused relocalization of CMCase. Together our data suggests a role for CMCase in early symbiosis.  相似文献   

Production of Oat Cakes from Extruded Pellets     

L. Han  H. E. Huff  F. Hsieh 《Cereal Chemistry》2008,85(4):522-529

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Parvalbumin characteristics in the sonic muscle of a freshwater ornamental grunting toadfish (Allenbatrachus grunniens)     

Kuo-Hsun Chiu  Fu-Ming Hsieh  Yu-Yun Chen  Hurng-Wern Huang  Jentaie Shiea  Hin-Kiu Mok 《Fish physiology and biochemistry》2013,39(2):107-119

The grunting toadfish, Allenbatrachus grunniens, is an ornamental fish in freshwater aquariums, and it has the ability to produce sounds. The sonic muscle of the toadfish is the fastest vertebrate muscle ever measured, and the rates of Ca²⁺ transport and cross-bridge dissociation are also the fastest. Parvalbumins (PAs) are Ca²⁺-binding proteins that help in muscle relaxation in vertebrates. Several PA isoforms have been identified in variable ratios in different muscle types. Both male and female grunting toadfish have intrinsic sonic muscles attached to their swim bladders, but no significant difference in morphology between male and female sonic muscles has been observed. In this study, we used SDS-PAGE and western blotting to characterize the total PA expression and to identify the PAs from the sonic muscle and the white body muscle of A. grunniens. Although the total PA concentrations were similar in sonic and white muscles, there were differences in the isoform percentages. Two and four PA isoforms were identified from sonic muscle and white muscle, respectively. The estimated sizes of PA1, PA2, and PA3 in the sonic muscle of the grunting toadfish were 10, 10.5, and 10.5 kDa, respectively, and the isoelectric points of PA1, PA2, and PA3 in the grunting toadfish were 4.77, 4.58, and 4.42, respectively. In the sonic muscle, the primary PA isoform was PA1, which comprised more than 94 % of total PA, whereas PA2 comprised only 5 % of the total PA content. In contrast, in white muscle, the primary isoform was PA2, which comprised 58 % of the total PA. Both PA1 (with PA1a) and PA3 represented approximately 20 % of the total PA in white muscle. These results indicate that there is no positive correlation between a high PA content and the speed of muscle relaxation; however, PA1 might have the greatest effect on the relaxation of the grunting toadfish’s sonic muscle.  相似文献   

Sound absorbent,flame retardant warp knitting spacer fabrics: Manufacturing techniques and characterization evaluations     

Yi-Jun Pan  Chien-Teng Hsieh  Chien-Lin Huang  Chen-Hung Huang  Ching-Wen Lou  Che-Wei Li  Jia-Horng Lin 《Fibers and Polymers》2015,16(12):2682-2688

This study proposes a combination for reciprocal reinforcement between warp knitting spacer fabrics and PU foams. PET/Kevlar nonwoven fabrics are made with an 80:20 ratio and an incorporation of various needle-punching speed of 100, 150, 200, 250, and 300 needles/min. Ascribing to having an optimal bursting strength, sound absorption coefficient, and limited oxygen index (LOI), the PET/Kevlar nonwoven fabric that is made by 200 needles/min are selected to be combined with a glass-fiber fabric by applying needle punch in order to form a surface layer. Next, warp knitting spacer fabrics and the nonwoven fabrics are laminated, followed by being combined with polyurethane (PU) foam that are featured with different densities of 200, 210, 220, 230, and 240 kg/m³ in order to form spacer fabric/PU foam composites with multiple functions. The composites are then tested with a drop-weight test, a compression test, a bursting strength test, a sound absorption test, and a horizontal burning test. The test results indicate that all spacer fabric/PU foam composites reach a horizontal burning level of HF1, and their sound absorption coefficients at 2500-4000 Hz also suggest a satisfactory sound absorption. In particular, the optimal residual stress and compressive strength are present when the composites contain 210 kg/m³ PU foam. Similarly, the optimal bursting strength of the composites occurs when they are composed of 230 kg/m³ PU foam. The spacer fabric/PU foam composites are proven to have high strengths, sound absorption, and fire retardant, and thus have promising potentials for use as construction materials and light weight composite planks.  相似文献   

Allotransplantation of Transgenic Mouse Ovaries Expressing Enhanced Green Fluorescent Protein under the Control of the Murine Phosphoglycerate Kinase 1 Promoter     

HT Wu  CK Chou  YC Hung  CK Yu 《Reproduction in domestic animals》2010,45(5):900-906

The aim of this study was to generate a transgenic mouse that ubiquitously expressed enhanced green fluorescent protein (EGFP) under the control of the murine phosphoglycerate kinase 1 promoter by allotransplantation of transgenic mouse ovaries. The EGFP transgenic mice expressed green fluorescence in many organs, and the fluorescence was detected as early as the embryonic stage. Ovaries from the EGFP transgenic mice were allotransplanted into recipients and these mice were mated with normal male mice. Histological sections of EGFP‐allotransplanted ovaries from the recipient mice showed the well development and formation at follicles and corpora lutea. The green fluorescence was clearly detectable at the allotransplanted section of the ovaries, which had fused with the normal ovary. The average size of the first litter from these mice was 6.8 ± 1.2 pups per recipient, and 17.8% of the pups expressed EGFP. These results demonstrated that allotransplantation of transgenic ovaries can restore a normal reproductive lifespan and can be used to generate a ubiquitously expressing EGFP animal model.  相似文献   

Fabrication of poly(vinyl alcohol) nanofibers by wire electrode-incorporated electrospinning     

Chien-Teng Hsieh  Ching-Wen Lou  Yi-Jun Pan  Chien-Lin Huang  Jia-Horng Lin  Yueh-Sheng Chen  Kun-Chien Chiang 《Fibers and Polymers》2016,17(8):1217-1226

Wire electrodes for needleless electrospinning consist of stainless steel wires in place of cylinder electrodes. The effects of different numbers of constituent stainless steel wires on the morphology and diameter of polyvinyl alcohol (PVA) fibers are examined. With 1, 2, 3, or 4 stainless steel wires being twisted as wire electrodes, an 8, 10, or 12 wt.% polyvinyl alcohol (PVA) solution is electrospun into PVA nanofibers by using a needleless electrospinning machine. The morphology and diameter of PVA nanofibers is observed by scanning electron microscopy. The combination of the number of stainless steel wires (two), PVA solution (10 wt.%), and the collecting distance (10 cm) results in the finest diameter and an evenly formed fiber morphology. In addition, the nanofibers exhibit a wide range of diameters when electrospun with an electrode consisting of more than two stainless steel wires. Compared with the cylinder electrode, the use of a wire electrode can form nanofibers, which results in a more even morphology.  相似文献   

银杏种核形状及其种仁成分的分析研究(英文)   总被引：2，自引：0，他引：2  

陈鹏  何凤仁  余碧钰  林协 《中国林学(英文版)》1999,(1)

根据银杏种核的形状将银杏雌株品种分为长子、佛指、马铃、梅核和圆子５ 种类型．测定分析结果表明,不同品种类型间的种核三维度,即长／ 宽、长／ 厚、宽／ 厚比具显著或极显著差异,其株间变异较小,种仁中氢氰酸含量与种核的宽／ 厚比呈极显著正相关;而不同品种类型间的单核重及种仁中与品质密切相关的干物质、支链淀粉、直链淀粉、淀粉、粗蛋白及可溶性糖含量差异不显著,但其株间变异较大,最大变异系数达到２４７９ ％ ．  相似文献   

Supercritical CO<Subscript>2</Subscript> extraction and concentration of n−3 polyunsaturated fatty acid ethyl esters from tuna cooking juice     

Chang?-Wei?Hsieh  Chiehming?J.?Chang  Wen-Ching?KoEmail author 《Fisheries Science》2005,71(2):441-447

Cooking is an important step in the tuna canning process; it usually produces functional solubilized proteins and lipids. The objective of this study was to recover and increase the concentration of functional n−3 fatty acids from the lipids in cooking juice. The lipids contained 12.98% eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) after ethyl esterification and increased to 37.4% with subsequent urea adduct fractionation. Supercritical carbon dioxide extraction was further used to increase the concentration of EPA and DHA ethyl esters. The ratio of EPA+DHA ethyl esters (high-molecular-weight components) to that of C₁₆ and C_18∶1 fatty acid ethyl esters (low-molecular-weight components) was used as a separation index for evaluating the process variables. Experimental results indicated that a high CO₂ density caused a low separation factor. At 1500 psig and 328.2 K, the extraction collected over 600 L CO₂, displaying an accepted concentration factor of EPA+DHA ethyl esters herein. About 80% yield of EPA and DHA was obtained and the ethyl esters increased from 37.4% to 54.3%.  相似文献   

Changes in oocyte and blood plasma osmotic components of ayu, Plecoglossus altivelis Temminck & Schlegel during oocyte maturation     

Ying-Nan Chen  Shu-Ling Hsieh  & Ching-Ming Kuo 《Aquaculture Research》2003,34(10):859-867

Oocyte maturation in teleosts is characterized by a complex series of cytoplasmic and nuclear changes. The purpose of this study is to present sequential changes in the oocyte constituents and regulatory mechanisms involved in the hydration and osmotic regulation of oocytes during final maturation of ayu, Plecoglossus altivelis Temminck & Schlegel. Oocyte volumes and water contents in ayu (P. altivelis) markedly increased from 0.372 mm³ and 60.5% at the mature stage to 0.712 mm³ and 79.4% at the time of oviposition. In the same period, net increases in total free amino acids and electrolytes were noted, and involvements of these constituents in the water uptake by oocytes are discussed. Moderate oocyte hydration in ayu is accompanied by steady increases in Na⁺, but not K⁺, and these observations are in contrast to results for all other marine teleosts reported elsewhere. In the present study, a steady increase in oocyte Na⁺ during maturation until ovulation pre‐dominantly results from diffusional leakage due to concentration gradients, while at the time of oviposition, both active transport and diffusional pathways play important roles in osmotic regulation in ayu oocytes.  相似文献   

Matrix solid phase dispersion isolation and liquid chromatographic determination of oxytetracycline in catfish (Ictalurus punctatus) muscle tissue     

A R Long  L C Hsieh  M S Malbrough  C R Short  S A Barker 《Journal of the Association of Official Analytical Chemists》1990,73(6):864-867

A method for isolation and liquid chromatographic determination of oxytetracycline in catfish (Ictalurus punctatus) muscle tissue is presented. Blank control and oxytetracycline-fortified fish muscle tissue samples (0.5 g) were blended with octadecylsllyl (C18, 40 microns, 18% load, endcapped) derivatized silica packing material (2 g) containing 0.05 g each of oxalic acid and disodium ethylenediaminetetraacetate. A column made from the C18/fish tissue matrix was first washed with hexane (8 mL), following which the oxytetracycline was eluted with acetonitrile-methanol (1 + 1, v/v) containing 0.06% w/v each of butylated hydroxyanisole and butylated hydroxytoluene. The eluate contained oxytetracycline analyte that was free from interfering compounds when analyzed by liquid chromatography with UV detection (photodiode array set at 365 nm). Standard curves for oxytetracycline isolated from fortified samples were linear (0.998 +/- 0.002) with an average absolute percentage recovery of 80.9 +/- 6.6% for the concentration range (50, 100, 200, 400, 800, 1600, and 3200 ng/g) examined. The interassay variability was 11.3 +/- 5.2% with an intra-assay variability of 1.1%.  相似文献