Antioxidant activities of phenolic acids on ultraviolet radiation-induced erythrocyte and low density lipoprotein oxidation     

Hsieh CL  Yen GC  Chen HY 《Journal of agricultural and food chemistry》2005,53(15):6151-6155

The exposure of mammalian cells to UV light induces various deleterious responses. Some of the major harmful effects are DNA damage, cell membrane peroxidation, systemic immune suppression, and aging acceleration. Reactive oxygen species and free radicals are believed to be largely responsible for some of the deleterious effects of UV upon cells. Typical administration of antioxidants has recently proved to represent a successful strategy for protecting the cells against UV-mediated oxidative damage. The objective of this study was to investigate the inhibitory effect of phenolic acids (caffeic acid, ferulic acid, gallic acid, and protocatechuic acid) on oxidative damage in human erythrocytes and low-density lipoprotein (LDL) induced by UVB radiation. The results revealed that the thiobarbituric acids reactive substances induced by UVB were decreased from 2.78 to 0.12-0.89 nmol MDA/mg protein in erythrocyte ghost and from 0.72 to 0.14-0.43 nmol MDA/mg protein in LDL by the addition of phenolic acids (100 muM). Caffeic acid, ferulic acid, and gallic acid exhibited over 85 and 60% inhibitory effect toward UVB-induced oxidation in erythrocytes and LDL, respectively. Phenolic acids, especially gallic acid, could maintain the normal glutathione levels and glutathione peroxidase activity in hemolysate from erythrocytes that were exposed to UVB radiation in comparison with untreated control. The results indicate that the antioxidant activities of caffeic acid and ferulic acid play a potential role in protection against UVB oxidative damage to human erythrocytes and LDL.  相似文献   

Institutional capacity and rural community planning in Japan: an event history analysis     

Li-Pei Peng  Yasuaki Kuki  Shizuka Hashimoto  Yeu-Sheng Hsieh 《Paddy and Water Environment》2014,12(1):55-69

Bottom-up and governance paradigms are becoming more prevalent in rural community planning in East Asia. Rural communities must enhance their institutional capacities, which are the baseline for planning future changes. However, few studies have analyzed the relationship between institutional capacity and rural community planning. Using a quantitative method of event history analysis, we compared the hazards of establishing conferences and plan approval from the Sato-dzukuri of Kobe City, Japan. We examined the effect of institutional capacity on rural community planning for the conference establishment and plan approval. We found that knowledge resources and relational resources are related to the proxy mobilization capacity for conference establishment. These resources are related to plan approval, although no relationship exists between conference establishment and plan approval. Conferences can be planned more rapidly than plans for approval can be, although both require substantial time. Communities with rural contexts present more rapid conference establishment than do suburban areas, whereas there are no significant differences in plan approval. This suggests that rural communities require more effort for the plan approval process. Rural community planning should judiciously address issues of institutional capacity regarding restrained knowledge resources and progress management, and should maintain vigilance regarding administration to achieve local governance.  相似文献   

Modified atmosphere and humidity packages for conservation of paper antiques     

Lang-Dong Lin  Ching-Min Hsieh  Been-Huang Chiang  Ming-Jer Tsai 《Journal of Wood Science》2007,53(2):121-126

Modified atmosphere and humidity (MAH) agents were developed to manipulate the gas composition and humidity for controlling the foxing of paper materials during storage. Sodium ascorbate, sodium carbonate decahydrate, ferrous sulfate heptahydrate, and silica gel were selected as the basic ingredients to formulate the MAH agents that could effectively remove oxygen, release carbon dioxide, and control the relative humidity (RH). With all the MAH agents developed in our study, RH was reduced and maintained without decreasing the MAH efficiency. To evaluate the inhibition of microorganisms on antique paper by MAH agents, the properties of Wikstroemia papers were measured after accelerated aging and inoculation with Aspergillus flavus and Penicillium citrinum. Under high (90%) or low (50%) RH conditions, as long as MAH agents were used, even after 80 days, the color difference value of Wikstroemia paper was kept below 1.5 and foxing was not found. Paper packaged without MAH agents and under RH as high as 90% showed a substantial color difference in 60 days. Snowflake-like foxing was also found by ultraviolet light inspection. Wikstroemia paper inoculated with P. citrinum without an MAH agent showed a significant color difference in 80 days.  相似文献   

Each species of Glycine collected in Taiwan has a unique seed protein pattern     

J.S. Hsieh  K.L. Hsieh  Y.C. Tsai  Y.I. Hsing 《Euphytica》2001,118(1):67-73

Several annual and perennial species in the genus Glycine Willd., including G. soja, long-pod G. tomentella, short-pod G. tomentella and G. tabacina, collected in Taiwan and nearby islands were studied for variations of their seed proteins. SDS-PAGE and Western blotting were used to analyze the total proteins, the heat soluble proteins, six seed maturation proteins (GmPMs) and one seed storage protein. The various species had different patterns of seed heat soluble proteins. In addition,each species of Glycine collected in Taiwan exhibited unique seed maturation protein patterns. They had several cross-reactive polypeptides recognized by specific antibodies against GmPM1, GmPM2 and GmPM8, but only one polypeptide recognized by antibodies against GmPM4, GmPM5 and MP130. The long pod G. tomentella, which has been suggested as a new species and renamed as G. dolichocarpa, could be distinct from the short pod G. tomentella on the basis of the analysis of these biochemical markers. It is also indicated that these GmPM antibodies may be used to distinguish between and within other Glycine species. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Effect of sugar on anthocyanin degradation and water mobility in a roselle anthocyanin model system using 17O NMR     

Tsai PJ  Hsieh YY  Huang TC 《Journal of agricultural and food chemistry》2004,52(10):3097-3099

The purpose of this study was to try to elucidate the relationship between anthocyanin degradation and water mobility using (17)O NMR. A model system containing anthocyanin from roselles with different sugar concentrations (20, 40, 60%) was used to compare the effect of sugar (sucrose and honey) on the kinetics of anthocyanin degradation and water mobility after heating. Data on the anthocyanin degradation index (DI), half-life of anthocyanin, and activation energy of anthocyanin degradation showed that sucrose was a good anthocyanin protector, especially at high concentration. However, honey led to a severe anthocyanin degradation after its concentration reached 40% or it was heated to temperatures >50 degrees C. Spin-spin relaxation rates (R(2)) of water using (17)O NMR were further used to monitor the water mobility in two sugar systems to explain the differences in browning under the same concentration and water activity. R(2) in sucrose was significantly higher than that in honey after the concentration reached 40%. Apparently, increasing the ability to bind with water molecules favored the stability of anthocyanin in sucrose solution.  相似文献   

Interactive effects of microbial transglutaminase and recombinant cystatin on the mackerel and hairtail muscle protein     

Jiang ST  Hsieh JF  Tsai GJ 《Journal of agricultural and food chemistry》2004,52(11):3617-3625

Interactive effects of microbial transglutaminase (MTGase) and recombinant cystatin on the mackerel and hairtail water soluble protein (WSP), salt soluble protein (SSP), and muscle protein (MP) were investigated. According to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and enzymic activity analyses, cross-linking of mackerel and hairtail myosin heavy chain and low molecular mass compounds and formation of epsilon-(gamma-glutamyl)lysine cross-links were observed on samples with MTGase, while the recombinant cystatin could effectively inhibit the cathepsins and subsequently prevent degradation of proteins during setting. The cathepsins and MTGase activities in WSP, SSP, and MP solutions decreased, but the recombinant cystatin activity increased during setting at 45 degrees C.  相似文献   

Characterization of cyclodextrin glycosyltransferase of the same gene expressed from Bacillus macerans, Bacillus subtilis, and Escherichia coli     

Jeang CL  Lin DG  Hsieh SH 《Journal of agricultural and food chemistry》2005,53(16):6301-6304

The plasmid pHG contains a cyclodextrin glycosyltransferase (CGTase) gene (cgt) derived from Bacillus macerans. Two transformants, Bacillus subtilis (pHG) and Escherichia coli (pHG), were found to produce CGTases with the same primary structure as the enzyme from B. macerans. However, the beta-cyclodextrin coupling activity of the CGTase from E. coli (pHG) was 14-fold higher than that of the enzymes from the other strains. By contrast, no differences in alpha-cyclodextrin coupling activities were observed among these CGTases. CGTase from E. coli (pHG) was found to be less thermostable than the other CGTases. When the CGTase produced by B. subtilis was treated with increasing urea concentrations (10-1000 mM) to promote increasing degrees of protein unfolding, a bell-shaped beta-cyclodextrin coupling activity profile was obtained. Subtle differences in the conformation of the CGTase produced by E. coli are therefore proposed to be responsible for the markedly increased beta-cyclodextrin coupling activity of this enzyme.  相似文献   

Inhibitory effects of Cassia tora L. on benzo[a]pyrene-mediated DNA damage toward HepG2 cells   总被引：4，自引：0，他引：4  

Wu CH  Hsieh CL  Song TY  Yen GC 《Journal of agricultural and food chemistry》2001,49(5):2579-2586

The effects of water extracts from Cassia tora L. (WECT) treated with different degrees of roasting on benzo[a]pyrene (B[a]P)-induced DNA damage in human hepatoma cell line HepG2 were investigated via the comet assay without exogenous activation mixtures, such as S9 mix. WECT alone, at concentrations of 0.1-2 mg/mL, showed neither cytotoxic nor genotoxic effect toward HepG2 cells. B[a]P-induced DNA damage in HepG2 cells could be reduced by WECT in a dose-dependent manner (P < 0.05). At a concentration of 1 mg/mL, the inhibitory effects of WECT on DNA damage were in the order unroasted (72%) > roasted at 150 degrees C (60%) > roasted at 250 degrees C (23%). Ethoxyresorufin-O-dealkylase activity of HepG2 cells was effectively inhibited by WECT, and a similar trend of inhibition was observed in the order unroasted (64%) > roasted at 150 degrees C (42%) > roasted at 250 degrees C (18%). The activity of NADPH cytochrome P-450 reductase was also decreased by unroasted and 150 degrees C-roasted samples (50% and 38%, respectively). Furthermore, glutathione S-transferase activity was increased by treatment with unroasted (1.26-fold) and 150 degrees C-roasted (1.35-fold) samples at 1 mg/mL. In addition, the contents of anthraquinones (AQs) in WECT, including chrysophanol, emodin, and rhein, were decreased with increasing roasting temperature. Each of these AQs also demonstrated significant antigenotoxic activity in the comet assay. The inhibitory effects of chrysophanol, emodin, and rhein on B[a]P-mediated DNA damage in HepG2 cells were 78, 86, and 71%, respectively, at 100 microM. These findings suggested that the decreased antigenotoxicity of the roasted samples might be due to a reduction in their AQs content.  相似文献   

Matrix solid phase dispersion isolation and liquid chromatographic determination of sulfadimethoxine in catfish (Ictalurus punctatus) muscle tissue     

A R Long  L C Hsieh  M S Malbrough  C R Short  S A Barker 《Journal of the Association of Official Analytical Chemists》1990,73(6):868-871

A method for the isolation and liquid chromatographic determination of sulfadimethoxine in catfish (Ictalurus punctatus) muscle tissue is presented. Blank control and sulfadimethoxine-fortified fish muscle tissue samples (0.5 g) were blended with octadecyisilyl (C18, 40 micrograms, 18% load, endcapped) derivatized silica packing material. A column made from the C18/fish tissue blend was first washed with hexane (8 mL), following which the sulfadimethoxine was eluted with dichloromethane (8 mL). The eluant contained sulfadimethoxine analyte that was free from interfering compounds when analyzed by liquid chromatography with UV detection (photodiode array, 270 nm). Standard curves for sulfadimethoxine isolated from fortified samples were linear (0.999 +/- 0.001) with an average relative percentage recovery of 101.1 +/- 4.2% for the concentration range (50, 100, 200, 400, 800, and 1600 ng/g) examined using sulfamethoxazole as the internal standard. The interassay variability was 10.7 +/- 8.2% with an intra-assay variability of 2.2%.  相似文献   

Matrix solid phase dispersion isolation and liquid chromatographic determination of five benzimidazole anthelmintics in fortified beef liver     

A R Long  M S Malbrough  L C Hsieh  C R Short  S A Barker 《Journal of the Association of Official Analytical Chemists》1990,73(6):860-863

A multiresidue method for isolation and liquid chromatographic determination of 5 benzimidazole anthelmintics (thiabendazole, oxfendazole, mebendazole, albendazole, and fenbendazole) in beef liver tissue is presented. Blank or benzimidazole-fortified liver samples (0.5 g) were blended with octadecylsilyl derivatized silica packing material (C18, 18% load, endcapped, 2 g). A column made from the C18/liver matrix was first washed with hexane (8 mL), following which the benzimidazoles were eluted with acetonitrile. The acetonitrile extract was then passed through an activated alumina column. The eluate contained benzimidazole analytes that were free from interfering compounds as determined by UV detection (photodiode array, 290 nm). Correlation coefficients of standard curves for individual benzimidazoles isolated from fortified samples, using internal standardization, were linear (0.996 +/- 0.002 to 0.999 +/- 0.001) with average relative percentage recoveries from 62.0 +/- 6.7 to 86.8 +/- 8.6% for the concentration range (100-3200 ng/g) examined. The interassay variability was 7.0 +/- 4.1 to 12.9 +/- 10.2% with an intra-assay variability from 2.2 to 4.0%.  相似文献