Reduced immunoreactivity of tyrosine hydroxylase in the hypothalamic paraventricular nucleus of the seizure sensitive gerbil     

Lee CH  Choi JH  Chung DW  Hwang IK  Yoon YS  Won MH  Lee IS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(6):645-648

We compared the immunoreactivity and numbers of tyrosine hydroxylase (TH) immunoreactive neurons and neuropil in the paraventricular nucleus (PVN) of the hypothalamus between the seizure sensitive (SS) and seizure resistant (SR) gerbils. The distributional pattern of TH immunoreactivity was similar in both groups: TH immunoreactivity was seen mainly in magnocellular neurons of the PVN. However, total TH immunoreactivity in the neurons and neuropil in the SS gerbils was significantly lower than that in the SR gerbils. In addition, the number of TH immunoreactive neurons in the SS gerbils was also much lower than those in the SR gerbils. These results indicate that SS gerbils have a low TH immunoreactivity in the hypothalamic PVN compared with that in SR gerbils.  相似文献   

浅谈饲料企业的执行力——执行过程篇     

杨纯纯  韩鹏飞 《饲料广角》2008,(20):48-49

国内众多专家、学者仁者见仁,从饲料企业的各个角度谈到了饲料企业执行力方面的问题,并提出了许多有针对性的建议。本篇文章,笔者根据多年对饲料企业的研究,从执行力执行的过程方面．对饲料企业指令下达的各成员要素进行分析．并对指令执行流程中的各参与要素进行初步研究．在此分析研究的基础上,从管理学角度给出一些合理化的建议,希望给相关的饲料企业在经营管理上能够有所帮助。  相似文献   

奥奶净对奶牛生产性能和血液生化指标的影响     

韩兆玉  金志红  魏永刚 《中国奶牛》2008,(3):7-9

选择45头泌乳前期奶牛,根据年龄、胎次、泌乳时间和产奶量进行配对,随机分为对照组、试验Ⅰ组和试验Ⅱ组,各组均饲喂奶牛场配制的全混合饲料,另外,试验Ⅰ组每天每头奶牛日粮中添加45g奥奶净,试验Ⅱ组每天每头奶牛日粮中添加60g奥奶净,研究奥奶净对奶牛生产性能和血液生化指标的影响。结果表明,与对照组相比,补饲45g／d和60g／d奥奶净的奶牛产奶量分别提高了2．34％和2．96％（P〈0．05）;乳脂率分别提高2．63％和6．72％;体细胞数分别下降了35．92％和42．55％（P〈0．05）;磷酸肌酸激酶有提高的趋势,乳酸脱氢酶呈下降趋势;奶牛血清IgG、SOD和GSH-Px含量均有不同程度的提高,而MDA呈下降趋势。表明饲喂奥奶净能够促进奶牛泌乳、提高机体免疫力和机体抗应激能力．并有促进营养物质代谢的趋势。  相似文献   

不同添加剂对拉伸膜裹包苜蓿青贮饲料体外消化率的影响     

许庆方  玉柱  李志强  韩建国  白春生  薛艳林  孙娟娟 《中国奶牛》2008,(3):13-16

为评价不同添加剂对拉伸膜裹包苜蓿青贮饲料养分体外消化率的影响,采用两步法测定体外干物质消化率（IVDMD）和体外粗蛋白消化率（IVCPD）,采用一步法测定体外中性洗涤纤维消化率（IVNDFD）和体外酸性洗涤纤维消化率（IVADFD）。结果表明,苜蓿青贮后的养分消化率低于原料;绿汁发酵液处理组拉伸膜裹包苜蓿青贮的IVDMD显著高于甲酸处理组（P〈0.05）;不同添加剂处理拉伸膜裹包苜蓿青贮饲料的IVCPD没有显著差异;甲酸处理组和绿汁发酵液处理组拉伸膜裹包苜蓿青贮饲料的IVNDFD和IVADFD显著低于对照处理组（P〈0.05）。结论表明,苜蓿青贮后,养分的消化率下降,不同添加剂对苜蓿纤维的消化率有影响。  相似文献   

克氏原螯虾在安康地区三种养殖条件下生长情况的比较     

吉红  曹福余  王毅  单世涛  王永军  武和平  刘超  王立新  刘海侠  成定北  李志安  李寒松  岳继海 《家畜生态》2008,(6):76-81

为了引进和推广克氏原螯虾养殖技术,于2007年9月、10月分别从湖北购入亲虾和幼虾在安康水产试验示范站进行水泥池、稻田和土池塘三种条件下养殖;于2008年5月每周或半周测量一次体长体重,分别进行体长体重关系、个体出现率、个体相对增重率和体重体长比值的分析。结果表明：1.稻田克氏原螯虾体长体重的关系为：雌虾,y=0.0184x3.1984,R2=0.9506,雄虾,y=0.0113x3.4626,R2=0.9611,且雌虾生长匀速,雄虾生长异速,符合体重体长形态参数y=a＊Lb;2根据个体出现率可知,水泥池和稻田生长的群体主要集中7.71cm-9.71cm之间,土池塘生长的群体主要集中在8.21cm-10.71cm之间,土池塘生长速度相对较快;根据生长集中区间的个体相对增重率可知,5月份稻田个体增重率最高,为24.13%,土池塘最低;体重、体长比值随着体长的变化趋势表明,稻田虾呈线性变化,水泥池和土池塘虾变化呈“S”型,趋向线性变化。可以认为,克氏原螯虾可在安康地区正常生长,且本试验条件下5月份稻田中克氏原螯虾生长最快。  相似文献   

Lesions associated with the plexus venosus subcutaneus collaris of pigeons with chlorophacinone toxicosis     

Sarabia J  Sánchez-Barbudo I  Siqueira W  Mateo R  Rollán E  Pizarrod M 《Avian diseases》2008,52(3):540-543

We describe lesions in 29 adult domestic pigeons (Columba livia) poisoned with chlorophacinone, an indandione anticoagulant rodenticide. Birds were found dead in the field and in dovecotes after a wide-area treatment against common voles (Microtus arvalis) based on the dispersion in the open field of wheat grain treated with 0.005% chlorophacinone. At necropsy, most pigeons showed crops full of red-colored wheat grain, extensive subcutaneous hematoma in neck and breast zones, and hemorrhages in lungs and the coelomic cavity. Chlorophacinone was determined in liver samples by high-performance liquid chromatography with ultraviolet detection, and the mean (range) concentration was 11.2 (1.48-50.1) microg/g. Pigeons have a venous subcutaneous plexus in the neck zone (plexus venosus subcutaneus collaris), which has been described as an erectile tissue with a thermoregulation function. This case report describes a relationship between the subcutaneous hemorrhagic lesions and the plexus venosus collaris.  相似文献   

Identification of a newly isolated avian infectious bronchitis coronavirus variant in China exhibiting affinity for the respiratory tract   总被引：1，自引：0，他引：1  

Liu S  Wang Y  Ma Y  Han Z  Zhang Q  Shao Y  Chen J  Kong X 《Avian diseases》2008,52(2):306-314

  相似文献   

Trichostatin A and nuclear reprogramming of cloned rabbit embryos     

Shi LH  Ai JS  Ouyang YC  Huang JC  Lei ZL  Wang Q  Yin S  Han ZM  Sun QY  Chen DY 《Journal of animal science》2008,86(5):1106-1113

To investigate the influence of histone deacetylases on nuclear reprogramming after nuclear transfer, we treated the cloned embryos with a histone deacetylase inhibitor, Trichostatin A (TSA). In the present study, global changes in acetylation of histone H3-lysine 14, histone H4-lysine 12, and histone H4-lysine 5 were studied in rabbit in vivo fertilized embryos, somatic cell nuclear transfer (SCNT) embryos, and TSA-treated SCNT embryos. From the pronuclear to the morula stage, the deacetylation-reacetylation changes in acetylation of histone H3-lysine 14 and histone H4-lysine 12 occurred in both fertilized embryos and TSA-treated cloned embryos; however, the distribution pattern in untreated cloned embryos failed to display such changes. More interesting, the signal of acetylation of histone H4-lysine 12 in cloned embryos was detected in both the inner cell mass and the trophectoderm, whereas TSA-treated cloned embryos showed the same staining pattern as fertilized embryos and the staining was limited to the inner cell mass. The histone acetylation pattern of TSA-treated SCNT embryos appeared to be more similar to that of normal embryos, indicating that TSA could improve nuclear reprogramming after nuclear transfer.  相似文献   

Experimental studies with foot-and-mouth disease virus type Asia-1, responsible for the 2005 epidemic in China     

Zhang Q  Li D  Liu X  Liu Z  Cai X  Wu G  Qi S  Yang S  Yan X  Shang Y  He J  Ma J  Li J  Ma W  Han R  Liu X  Zhang J  Xie Q  Zhang Z 《Research in veterinary science》2008,85(2):368-371

This study was carried out to investigate the biological characteristics of the foot-and-mouth disease (FMD) virus strain Asia-1 China/2005, which is responsible for the 2005 epidemic in China. The result showed that this strain is not host restricted, and could not only cause FMD in cattle and sheep but also in pigs by either inoculation or direct contact.  相似文献   

表达牛乳铁蛋白肽的重组鸡源乳酸杆菌抗IBDV感染的研究     

林庆宇  师一鸣  宋丽影  李雪纯  谭宏超  于淑媛  姜艳平  崔文  乔薪瑗  王丽  周晗  徐义刚  李一经  唐丽杰 《中国预防兽医学报》2019,(4):402-407

为探究表达牛乳铁蛋白肽的重组鸡源乳酸杆菌pPG-XLFEC/M11对雏鸡抵抗传染性法氏囊病病毒(IBDV)感染的作用,本研究利用IBDVCEF94株感染DF-1细胞,通过CCK-8法检测病毒感染后的细胞活性,利用qRT-PCR检测病毒载量,结果显示重组乳酸菌表达的牛乳铁蛋白肽可以抑制IBDV在DF-1细胞内的增殖;以重组乳酸菌pPG-XLFEC/M11饲喂雏鸡,利用IBDV强毒UK661株进行攻毒实验,结果显示饲喂重组菌的雏鸡相比于对照组,IBDV的感染对其组织器官的损伤较小,总免疫球蛋白IgG和SIgA含量增加,细胞因子IL-6、IL-8、TNF-琢和IFN-酌以及TLR2的mRNA转录水平均显著升高,且提高了雏鸡的存活率。本实验结果表明,重组鸡源乳酸杆菌表达的牛乳铁蛋白肽可以增强机体的免疫应答水平,对雏鸡具有一定的抗IBDV感染的作用。本研究为表达牛乳铁蛋白肽的重组鸡源乳酸杆菌在生产中的应用以及为禽类疾病的预防奠定基础。  相似文献