Pewenomyces kutranfy gen. nov. et sp. nov. causal agent of an important canker disease on Araucaria araucana in Chile     

Felipe Balocchi  Mike J. Wingfield  Rodrigo Ahumada  Irene Barnes 《Plant pathology》2021,70(5):1243-1259

Araucaria araucana, (commonly referred to as araucaria, pewen, or monkey puzzle tree) is an ancient conifer endemic to the Chilean and Argentinian mountain ranges where it has a sacred relevance to indigenous communities. During 2015, a serious disease was noticed on trees of all ages in most of the natural distribution of this iconic tree. Four areas were surveyed, and the most important symptoms of the disease were cankers on branches and stems resulting in copious resin exudation. Trees were monitored for a period of two years and isolations were made from the cankers. Field observations showed that the disease typically begins on the leaves or at the leaf bases and progresses downwards to initiate cankers that can girdle branches or stems within a two-year period. Black ascomata, resembling those of Caliciopsis species previously described from A. araucana, were consistently found developing in the cankers from which isolations were made. Phylogenetic analyses of the ITS, nucSSU, and nucLSU gene regions showed that the fungus resides in the Coryneliaceae but is distinct from other genera in that family. The morphological characteristics and phylogenetic position of the fungus show that it represents a new genus and species, described here as Pewenomyces kutranfy gen. nov. et sp. nov. Pathogenicity trials on trees under field conditions confirmed that this newly described fungus is able to cause cankers on A. araucana similar to those found under natural conditions.  相似文献   

Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia     

Katarína Adamčíková  Zuzana Jánošíková  Ariska van der Nest  Slavomír Adamčík  Emília Ondrušková  Irene Barnes 《Plant pathology》2021,70(8):1883-1896

Dothistroma pini is one of two pathogens causing Dothistroma needle blight (DNB), a foliar disease of pines. The species was redefined in 2004 and subsequently recorded in several European countries. In Slovakia, the first report of the pathogen was in 2013. In this study, the population structure, genetic diversity, and reproductive mode of 105 isolates collected from 10 localities and seven hosts were determined in Slovakia. Species-specific mating type markers, ITS haplotype determination, and 16 microsatellite markers were used to characterize and genotype the isolates. Overall, 15 unique multilocus haplotypes (MLHs) based on microsatellite markers and three ITS haplotypes were identified. Three independent methods (DAPC, STRUCTURE, EDENetwork) separated the isolates into two distinct population clusters corresponding with ITS haplotypes. A high level of clonality was recorded suggesting that conidia are the primary source of pathogen dispersal. The low genetic diversity, predominantly asexual reproductive mode of the pathogen, and the fact that most isolates were collected from introduced tree species and native species in artificially planted urban greenery, supports the hypothesis that D. pini has been recently introduced into Slovakia.  相似文献   

Effect of Flavan-3-ols on the Adhesion of Potential Probiotic Lactobacilli to Intestinal Cells     

I Bustos  T García-Cayuela  B Hernández-Ledesma  C Peláez  T Requena  MC Martínez-Cuesta 《Journal of agricultural and food chemistry》2012,60(36):9082-9088

The effect of dietary flavan-3-ols on the adhesion of potential probiotic lactobacilli strains to intestinal cells was unraveled. The inhibitory activity of these compounds on intestinal cells was highlighted. The cytotoxic effect was shown to depend on both the compound's chemical structure (galloylation and polymerization) and degree of differentiation of intestinal cells. The effect of flavan-3-ols on bacteria adhesion differed greatly between compounds, strains, and intestinal cells. All flavan-3-ols inhibited significantly Lactobacillus acidophilus LA-5 and Lactobacillus plantarum IFPL379 adhesion except epigallocatechin gallate, which enhanced L. acidophilus LA-5 adhesion to Caco-2. Procyanidins B1 and B2 increased remarkably the adhesion of Lactobacillus casei LC115 to HT-29 cells, whereas epigallocatechin increased L. casei LC115 adhesion to Caco-2. These data showed the potential of flavan-3-ols to alter gut microecology by modifying adhesion of lactobacilli strains to intestinal cells.  相似文献   

New anthocyanidin and anthocyanin pigments from blue plumbago     

Skaar I  Jordheim M  Byamukama R  Mbabazi A  Wubshet SG  Kiremire B  Andersen OM 《Journal of agricultural and food chemistry》2012,60(6):1510-1515

Phytochemical investigations of blue plumbago ( Plumbago auriculata Poir. syn. Plumbago capensis Thunb.) flowers have led to the isolation of six new anthocyanins based on three new anthocyanidins with 5,7-dimethoxylated A-rings. Their structures were identified by 2D nuclear magnetic resonance and high-resolution mass spectrometry as the 3-O-β-galactopyranosides (1,2,4) and 3-O-α-rhamnopyranosides (3,5,6) of 5,7-dimethyldelphinidin, 5,7-dimethylpetunidin, and 5,7-dimethylmalvidin. Identification of 1-6 implies new structures for the previously reported anthocyanidins pulchellidin, europinidin, and capensinidin to be 5,7-dimethoxy-3,3',4',5'-tetrahydroxyflavylium, 5,7,3'-trimethoxy-3,4',5'-trihydroxyflavylium, and 5,7,3',5'-tetramethoxy-3,4'-dihydroxyflavylium cations, respectively. The anthocyanins (0.4 mg/g flowers) were accompanied by the dihydroflavonol taxifolin 3'-O-β-glucopyranoside (1.4 mg/g) and the flavonols 5-methylquercetin 3-O-α-rhamnopyranoside (8.8 mg/g) and 5-methylquercetin (0.4 mg/g). The anthocyanins 1-6 are the first reported natural anthocyanins with no free hydroxyl groups in their 5- and 7-positions on their A-rings. They have thus no possibility of forming the tautomeric quinonoidal bases (anhydrobases), which are related to the free hydroxyl groups in the 5- and 7-positions of previously reported anthocyanins. The genes behind the 5,7-dimethoxylated anthocyanins might be useful for making anthocyanins with special properties (colors, etc.).  相似文献   

Design aspects of 24 h recall assessments may affect the estimates of protein and potassium intake in dietary surveys     

Crispim SP  Geelen A  Siebelink E  Huybrechts I  Lillegaard IT  Margaritis I  Rehurkova I  Slimani N  Ocke MC  de Boer E  Veer PV  de Vries JH 《Public health nutrition》2012,15(7):1196-1200

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Reproduction of postweaning multisystemic wasting syndrome in pigs experimentally inoculated with a Swedish porcine circovirus 2 isolate.     

Gordon Allan  Francis McNeilly  Brian Meehan  Irene McNair  John Ellis  Steve Krakowka  Caroline Fossum  Eva Wattrang  Per Wallgren  Brian Adair 《Journal of veterinary diagnostic investigation》2003,15(6):553-560

In recent years, porcine circovirus type 2 (PCV2)-associated postweaning multisystemic wasting syndrome (PMWS) has been reported worldwide. However, to date, PMWS has not been reported in Sweden despite the demonstration of serum antibodies to a PCV2-like virus in Swedish pigs. This communication reports the experimental reproduction of clinical PMWS after inoculation of colostrum-deprived (CD) pigs, derived from a Northern Ireland herd, with an isolate of PCV2 virus recovered from a clinically normal Swedish pig that was necropsied in 1993. The clinical disease and histological lesions observed in CD pigs inoculated with this virus were indistinguishable from those observed in previous studies on CD pigs inoculated with a PCV2 virus isolate recovered from pigs with PMWS. These results highlight the disease potential of PCV2 isolated from regions apparently free of PMWS and suggest that the status of the host and its environment is an important factor in the development of clinical PMWS.  相似文献   

Resynchronization with the G6G protocol: A retrospective,observational study of second and later timed artificial inseminations on commercial dairy farms     

Raquel Patron  Irene Lopez‐Helguera  Jose Luis Pesantez‐Pacheco  Natividad Perez‐Villalobos  Javier Heras  Juan Vicente Gonzalez  Octavi Fargas  Susana Astiz 《Reproduction in domestic animals》2019,54(2):243-251

We recorded conception rates and estimated pregnancy rates following second and later timed artificial inseminations (TAIs) after hormonal resynchronization on commercial dairy farms, using the so‐called G6G protocol (PGF day‐0; GnRH 2, 8 days; PGF 15, 16 days, GnRH 17 days; TAI 18 days), and the 5‐day Ovsynch protocol or 5DO (GnRH day 0; PGF 5, 6 days; GnRH 7 days; TAI 8 days). In four farms, both protocols were implemented in parallel, and these 1,368 s and later TAIs were used for the protocols’ comparison based on logistic regression (544 TAIs in primiparous; 824 in multiparous cows; 1,024 TAIs after G6G [600 TAIs in multiparous and 424 in primiparous]; 344 TAIs after 5DO [224 TAIs in multiparous and 120 in primiparous]; 280 TAIs during the hot season; 1,088 during the cool season). Conception rate (CR) was 31.7% ± 12.0% among all cows, 35.1% ± 10.7% among cows resynchronized with the G6G protocol and 21.8% ± 9.7% among cows resynchronized with the 5DO protocol (p < 0.0001). CR among all cows was lower during the hot season (19.3% ± 8.4%) than during the cool season (34.9% ± 10.6%; p < 0.0001), and similar seasonal results were observed with G6G protocols. Logistic regression showed significant effects on CR in second and later TAIs by protocol (OR = 0.514; 95% CI 0.385–0.686; p < 0.0001) and season (OR = 0.486; 95% CI 0.350–0.676; p < 0.0001). Parity did not influence CR after second and later TAIs (p > 0.1), and no interaction with season or resynchronization protocol was found. Estimated pregnancy rates based on these CR data from both hormonal protocols suggest that G6G can be effectively used for second and later TAIs and highlight the importance of considering protocol and season when designing strategies for second and later timed AIs on dairy farms.  相似文献   

Effects of melatonin on oocyte developmental competence and the role of melatonin receptor 1 in juvenile goats     

Sandra Soto‐Heras  Maria‐Gracia Catal  Montserrat Roura  Irene Menndez‐Blanco  Anna‐Rita Piras  Dolors Izquierdo  Maria‐Teresa Paramio 《Reproduction in domestic animals》2019,54(2):381-390

Melatonin enhances in vitro embryo development in several species by improving the oocyte developmental competence during in vitro maturation (IVM). Melatonin has a wide range of actions, from scavenging reactive oxygen species (ROS) to regulating gene expression, and it can also act by way of melatonin receptors. The aim of this study was to determine the mechanism of action of melatonin during the IVM of juvenile goat oocytes and the role of the membrane receptors. Melatonin receptor 1 was immunolocalized in cumulus cells and oocytes before and after 24 hr of IVM. The effect of melatonin on oocyte developmental competence was tested in three experimental IVM groups: (a) control, (b) 10^?7 M melatonin, and (c) 10^?7 M melatonin +10^?7 M luzindole (an inhibitor of both melatonin receptors). After IVM oocytes were assessed for ROS levels, mitochondrial activity, adenosine 5′‐triphosphate (ATP) concentration and relative gene expression (ACTB, SLC1A1, SOD1, GPx1, BAX, DNMT1, GCLC and GDF9). IVM‐oocytes were in vitro fertilized and cultured under conventional conditions. Blastocyst rate and quality (differential cell count) were assessed at 8 days post‐fertilization. Melatonin decreased ROS levels, increased mitochondrial activity and ATP content and increased blastocyst quality compared to control group (55.8 vs. 30.4 inner cell mass ICM, p < 0.05). There was no effect on the relative gene expression due to treatment with melatonin. In conclusion, we have showed that melatonin improves oocyte developmental competence in juvenile goats by reducing ROS levels and improving mitochondrial activity.  相似文献   

Nutrition education and Mediterranean diet: exploring the teaching process of a school-based nutrition and media education project in Cretan primary schools     

Kafatos I  Peponaras A  Linardakis M  Kafatos A 《Public health nutrition》2004,7(7):969-975

BACKGROUND: During the past few decades there has been a gradual abandoning of the traditional Mediterranean diet by the population of Crete and especially among the younger generations. Since this is related to the rapidly increasing morbidity and mortality rates from chronic diseases, the need for educating young people on the principles of good nutrition becomes increasingly important. It is also acknowledged that the epidemiological field needs to focus on studying the implementation process, since this will allow for a deeper understanding of the outcomes. OBJECTIVES: The aims of the present study were to explore the process of implementing an innovation in Cretan primary schools and to identify best teaching practices and principles. DESIGN: In three state primary schools on the island of Crete, 107 children took part in a 30-h nutrition and media education programme. Classroom observations were used to define attitudes towards the programme and teaching methods. Besides baseline and post evaluation, the pupils were also interviewed about 11 months after the end of the programme. IMPLEMENTATION: During observation of the teaching process, emerging issues were the teacher's motivation and interest in the course, his/her preparation before each class, teaching ability and communication skills, the respect and discipline he/she kept and the innovative teaching methods used. In some cases researcher intervention in the classroom could not be avoided. FINDINGS AND CONCLUSIONS: Overall, individual teacher willingness and teaching skills were mainly responsible for the quality of teaching, the variety of educational methods used in classrooms and pupils' interest in the course. Changes in pupils' knowledge and recall of the course were related to teacher enthusiasm but also to researcher intervention. Exploring the teaching and learning process allowed a deeper understanding of the data. It is concluded that attempts to introduce the principles of a Mediterranean diet to children through nutrition education require innovative, enthusiastic and highly motivated teachers.  相似文献   

Water uptake by black spruce seedlings from rooting media (solution,sand, peat) treated with inorganic and oxalated aluminum     

Paul A. Arp  Irene Strucel 《Water, air, and soil pollution》1989,44(1-2):57-70

Experiments with solution cultures revealed that 1-yr-old black spruce (Picea mariana [Mill.]) seedlings growing in nutrient solutions experienced reductions in water uptake and moist plant weight when treated with water-soluble inorganic Al at the time of rapid shoot elongation. These reductions occurred with solution concentrations for Al at ≥ 16 mg L^?1. Wilting was noticed with Al ≥ 8 mg L^?1. In contrast, oxalated Al treatments had no visible effects on either water uptake or moist plant weight during shoot elongation. Later, near the time of budset, neither inorganic nor oxalated Al had an effect on moist plant weight or water uptake. This suggests that black spruce seedlings may or may not be susceptible to water-soluble inorganic Al depending on the degree of Al complexation afforded by the rooting medium, and by the plant roots at different times of the growing season. Water uptake was affected by the type of rooting medium in which the seedlings grew. For example, water uptake from peat was greater than water uptake from solution. Water uptake from sand was intermediate. Water uptake from solution-only cultures was probably affected by restricted O₂ supply.  相似文献