抗光肩星天牛苏云金芽胞杆菌Bt886菌株的分离及对毒蛋白编码基因的初步鉴定   总被引：6，自引：0，他引：6  

陈军  杜孟芳  尹新明  王学聘  卢孟柱 《林业科学》2004,40(5):138-142

从拟谷盗虫尸中分离到一株对鞘翅目害虫光肩星天牛幼虫毒杀作用明显的Bt菌株Bt886。幼虫死亡率高达 6 0 % ,存活者生长明显受到抑制。经过晶体形态特征和杀虫谱分析 ,初步确认该菌株属于cry3类。比较cry3类基因的保守序列 ,利用一对cry3特异引物成功地从Bt886菌株中克隆到了一段基因片段并克隆至pGEM -T载体上。测序分析发现该片段与cry3Aa1基因具有很高的同源性 ,确定来自毒蛋白编码基因。Southern杂交分析表明该基因位于该菌株的质粒上。  相似文献   

片石砂上发展用材林、经济林效益调查分析     

吕锦水 《浙江林业科技》1995,(6)

片石破土上发展用材林、经济林，在适地适树、集约经营的条件下，湿地松、杉木用材林每公顷年均产值在３０００元以上，柑桔、板栗等经济林木在７５００元以上；在同一立地条件上，用材树种的生长效益排列顺序是：国外松、木荷、马尾松，杉木只能在土层深厚的山坞、山脚小面积造林，经济林必需实行高标准、高投入，才能取得好效益，树种以柑桔、板栗、枇杷、桃形李等为主。  相似文献   

2000年世界林业产业政策的发展趋势     

陆文明 《世界林业研究》1995,8(5):1-8

本文详尽地论述了２０００年世界林业产业政策的发展趋势：继续实施林业持续发展战略；林业管理体制向促进发挥多种效益和集约化经营的方向发展；进一步采取大力发展人工林的政策；继续执行对林业的扶持政策；继续实施优化林业产业结构政策。文章还从国际范围角度阐述了林业持续发展的艰巨性。  相似文献   

香菇多糖提取纯化研究   总被引：8，自引：0，他引：8  

卢成英  李国章  黄早成  郭晓辉  曹庸 《中国林副特产》2006,(4):5-7

用0.2mol/L三氯乙酸、0.2mol/L氢氧化钠、1.0mol/L乙酸、蒸馏水为提取溶剂,结合超声波处理等方法从香菇子实体中提取香菇多糖,提取液经甲醇除杂、沉淀处理得粗多糖。粗多糖用CTAB络合沉淀,沉淀物乙酸溶解后取上清再用甲醇沉淀,沉淀物冻干得纯化多糖。分析检测结果显示干香菇三氯乙酸提取的纯化多糖的含量高达93.1%、提取得率达0.324%。薄层分析表明,纯化香菇多糖由葡萄糖、甘露糖、半乳糖、木糖组成。纯化多糖用Sephadex G-100凝胶层析,洗脱液经苯酚-硫酸法检测为单一吸收峰,红外光谱显示其具有多糖类的特征吸收峰。  相似文献   

微量元素锌在金针菇培养中的应用研究   总被引：3，自引：0，他引：3  

陆晓民  李世军 《中国林副特产》2006,(5):8-10

采用单因素完全随机设计，于金针菇培养期间用不同浓度的硫酸锌溶液对培养料进行处理，研究微量元素锌对金针菇的茵丝生长、子实体产量及其多糖含量、富锌量等的影响。试验结果表明：0．1～100mg／L的硫酸锌处理对茵丝生长均有促进作用，以10mg／L处理的茵丝生长速度最快；10mg／L和100mg／L的硫酸锌处理对子实体具有明显的增产效果；不同浓度的硫酸锌溶液处理均可提高金针菇子实体多糖及其富锌量，以100mg／L处理的多糖含量最高、富锌量最大。采用不同浓度的硫酸锌溶液对培养料进行处理时浓度以100mg／L为佳。  相似文献   

未来的人居建筑及其设计策略     

吴健梅  陆明 《森林工程》2003,19(6):67-68,39

本文研究了未来的人居建筑的发展趋势及其3个主要特点，即智能型、有机性和生态化。并且从建筑师的角度提出了相应的设计策略。  相似文献   

亚麻屑刨花板生产线工艺设计特点   总被引：1，自引：0，他引：1  

濮安彬  陆仁书 《木材工业》1996,10(2):11-15

本文以１．００万ｍ＾３／ａ的生产规模为例，介绍亚麻屑刨花板生产线的工艺特点和设备选型。  相似文献   

THE QUICK DETERMINATION METHOD OF RESIN CONTENT IN LARCH WOOD     

Lu Wenda Xu Xiuwen Zhao Dejun Tan Haiyan Northeast Forestry University 《林业研究》1992,3(1):62-67

The resin content in larch wood is usually determined by the traditionalBenzene-Alcohol Extract Method(BAEM).In order to meet the requirement of production prac-tice,the authors present two simple quick determination methods,IR lamp method and hydrogenperoxide solution method.Compared with the results of traditional method,the results of newmethods are nearly the same as former,therefore,both two methods can reflect the change condi-tion of resin content in wood.  相似文献   

In vitro plantlet formation from embryonic explants of eastern white cedar (Thuja occidentalis L.)     

Harry IS  Thompson MR  Lu CY  Thorpe TA 《Tree physiology》1987,3(3):273-283

A protocol is described for the in vitro production of plantlets from embryonic explants of eastern white cedar (Thuja occidentalis L.). Bud induction was optimal when embryonic explants were cultured for 20-25 days on half-strength Quoirin and Le Poivre mineral salts containing equimolar concentrations (10(-6) M) of N(6)-benzyladenine and 2-isopentyl adenine. Bud development was achieved in phytohormone-free medium in the presence of activated charcoal. Maximal shoot elongation occurred on half-strength Quoirin and Le Poivre salts, whereas shoot multiplication was optimal on half-strength Bornman's MCM salts in the presence of cytokinin. Hardened shoots, dipped in commercial rooting powder containing indole-3-butyric acid, rooted optimally in mist under non-sterile greenhouse conditions. Both rooting and subsequent plantlet growth was best when Redi-Earth((R)) was used as a substrate. Over 250 plantlets per embryo can be produced annually by this technique.  相似文献   

Carbon return and dynamics of litterfall in natural forest and monoculture plantation of Castanopsis kawakamii in subtropical China     

Guo Jianfen  Xie Jinsheng  Lu Haoliang  Liu Dongxia  Yang Yusheng  Chen Guangshui 《中国林学(英文版)》2004,6(1):33-36

The amount of carbon returned through litterfall and its seasonal pattern were studied in a natural forest of Castanopsis kawakamii (NF) and adjacent monoculture plantations of C. kawakamii (CK) and Chinese fir (Cunninghamia lanceolata) (CF) in Sanming, Fujian Province, China. Mean annual carbon return through total litterfall over 3 years (from 1999 to 2001) was 5.097 t·hm⁻² in the NF, 4.337 t·hm⁻² in the CK and 2.502 t·hm⁻² in the CF respectively. Of the total carbon return in the three forests, leaf contribution accounted for 58.96%, 68.53% and 56.12% and twig 24.41%, 22.34% and 26.18%, respectively. The seasonal patterns of carbon return from total litterfall and leaf-litter were quite similar among the three forests. A peak of carbon input from litterfall in the NF and the CK occurred in spring except for the highest annual C return through branch litter of the NF in summer, while the CF showed the maximum C return in summer. The results of this study demonstrate that the natural forest has a greater C return through litterfall than monoculture plantations, which is beneficial to the increase of soil organic matter storage and the maintenance of soil fertility. [Supported by the Teaching and Research Award Program of MOE P.R.C. (TRAPOYT) and the Key Basic Research Project of Fujian Province (2000F004)]  相似文献