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Lunasin is a novel, cancer-preventive, anti-inflammatory and cholesterol-reducing peptide that was originally isolated from soy and later from barley, wheat and rye. We report the first discovery of lunasin in triticale (X Triticosecale Wittmack). Moreover, we report first data of lunasin content in winter rye and wheat genotypes grown in Northern Europe. These data are novel as previously published data on finding of lunasin in cereals were obtained in genotypes grown in Korea. Lunasin content was uncovered using a previously published procedure for isolation from cereals and identified by LC-MS/MS assay. We found that triticale was the most lunasin-rich cereal, with the tested genotypes displaying the following trend in lunasin content: genotype 0002-26 > Dinaro > DSGU 10/94 > 0213-22 > 0317-14 > 0006-31. The greatest lunasin content was 6.46 mg/g in the grain of triticale genotype 0002-26. In comparison, the highest lunasin content in rye variety Dankovske Diament was 1.5 mg/g, and the highest lunasin content in the winter wheat variety Fredis was 0.23 mg/g. We conclude that triticale can play a significant role as functional food, with great potential for the use of triticale products in human and animal diets.  相似文献   
46.
We synthesized nano-scaled periodic ripple patterns on silicon and titanium dioxide (TiO(2)) surfaces by xenon ion irradiation, and performed adsorption experiments with human plasma fibrinogen (HPF) on such surfaces as a function of the ripple wavelength. Atomic force microscopy showed the adsorption of HPF in mostly globular conformation on crystalline and amorphous flat Si surfaces as well as on nano-structured Si with long ripple wavelengths. For short ripple wavelengths the proteins seem to adsorb in a stretched formation and align across or along the ripples. In contrast to that, the proteins adsorb in a globular assembly on flat and long-wavelength rippled TiO(2), but no adsorbed proteins could be observed on TiO(2) with short ripple wavelengths due to a decrease of the adsorption energy caused by surface curvature. Consequently, the adsorption behavior of HPF can be tuned on biomedically interesting materials by introducing a nano-sized morphology while not modifying the stoichiometry/chemistry.  相似文献   
47.
Two experimental trials were performed to elucidate the role of rodents in the life cycle of Hepatozoon species using snakes as intermediate hosts. In one trial, two ball pythons, Python regius Shaw, 1802 were force fed livers of laboratory mice previously inoculated with sporocysts of Hepatozoon ayorgbor Sloboda, Kamler, Bulantová, Votypka et Modry, 2007. Transmission was successful in these experimentally infected snakes as evidenced by the appearance of intraerythrocytic gamonts, which persisted until the end of trial, 12 months after inoculation. Developmental stages of haemogregarines were not observed in histological sections from mice. In another experimental trial, a presence of haemogregarine DNA in mice inoculated with H. ayorgbor was demonstrated by PCR in the liver, lungs and spleen.  相似文献   
48.

Key message

The position of trees in the canopy impacts xylem structure and its inter-annual variation. After canopy release, the increase in the hydraulic conductivity of growth rings was driven by an increase in radial growth in large trees, and by both an increase in radial growth and changes in xylem structure in saplings.

Context

Forest canopies are frequently subjected to disturbances that allow understory trees to access the upper canopy. The effect of canopy release on xylem anatomy has been assessed in juvenile trees and saplings, while the potential acclimation of larger trees remains poorly documented.

Aims

We estimated the potential hydraulic conductivity of growth rings in large understory trees compared to overstory trees, and evaluated the responses to canopy release in large trees and in saplings.

Methods

We recorded radial growth, wood density, and vessel structure in beech trees according to their position within the canopy and their size. Xylem traits were followed during 6 years after canopy release for large trees, and during 2 years for saplings. Vessel diameter and frequency as well as ring area were used to compute the potential annual ring hydraulic conductivity.

Results

Large understory trees displayed lower radial growth increments and lower potential annual ring hydraulic conductivity than overstory trees. After canopy release, potential annual ring hydraulic conductivity increased in large trees, due exclusively to increased radial growth without any change in specific hydraulic conductivity. It increased in saplings due to both increased radial growth and increased specific conductivity.

Conclusion

Tree size impacted xylem structure and resulted in plasticity of the potential hydraulic conductivity of the annual tree ring following canopy release.
  相似文献   
49.
To assess the effects of three insecticides (aldicarb, chlorpyrifos, deltamethrin) and two fungicides (tebuconazole and metalaxyl + mancozeb) on the PCR-DGGE fingerprints of culturable soil bacterial communities (CSBC), a greenhouse experiment was carried out with soil samples from an Integrated System for Agroecological Production (ISAP), a Conventional Potato Production Area (CPPA) and a Secondary Forest Area (SFA) close to the CPPA. Samples were obtained at 15 day intervals starting at 32 until 77 days after sowing (DAS) to perform the PCR-DGGE analysis of the CSBC cultured on media amended with soil suspension. Analysis of variance from PCR-DGGE data indicated significant differences among treatments. Regardless the type of pesticide applied, CSBC was disturbed and similarity values varied from 5% to 90% in comparison to the control. Significant shifts on CSBC were only detected among treatments in the first two harvests, while CSBC tended to be more akin to each other at the last two harvest dates. The most significant responses observed were due to different soil sample origins, where values of 5% of similarity to the control were observed on CPPA soil. The use of analysis of variance on PCR-DGGE data was useful to a better understanding of the changes on CSBC induced by pesticides applications.  相似文献   
50.

The ability to determine the presence and identity of sugars in the guts of adult parasitoids in the field would aid researchers in addressing long-standing problems in parasitoid ecology. Until very recently, however, gut sugar analyses have not been carried out on parasitoids. This is despite the development and use of methodologies for gut sugar analyses in biting flies (mosquitoes, sand-flies, black-flies, horse- and deer-flies, and biting midges) for decades. Methods used have been the cold anthrone test for the detection of gut sugars, and various forms of chromatography for the identification of gut sugars. We review the use of these methods in biting fly research and then describe the nascent field of gut sugar analyses in parasitoids. Both cold anthrone and chromatography tests have begun to be used on field-caught parasitoids, and we describe progress from our own work. We used cold anthrone on the aphid parasitoid Aphelinus albipodus (Hymenoptera: Aphelinidae), and results from one field study show that approximately one-fifth of individuals tested were positive for gut sugars. The characteristics of the field site point to the primary source of these gut sugars as being aphid honeydew. We also analysed the gut contents of Diadegma insulare (Hymenoptera: Ichneumonidae), a parasitoid of diamondback moth. In this case, HPLC analyses showed that over 85% of field-captured individuals had fed upon sugars. These same analyses suggested that honeydew may have been a major source of the gut sugars in this case also, but the sugar profiles suggest some nectar feeding. Understanding the importance of various sugar sources on parasitoid activity and effectiveness will facilitate the incorporation of sugar sources in habitat manipulation programmes as a part of IPM.  相似文献   
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