Acylated flavonol glycoside from Platanus orientalis     

Tantry MA  Akbar S  Dar JA  Irtiza S  Galal A  Khuroo MA  Ghazanfar K 《Fitoterapia》2012,83(2):281-285

The ethylacetate and n-butanol fractions of ethanolic extract of Platanus orientalis leaves led to the isolation of new acylated flavonol glycoside as 3',5,7-trihydroxy-4'-methoxyflavonol 3-[O-2-O-(2,4-Dihydroxy)-E-cinnamoyl-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranosyl (1→2)]-β-D-glucopyranoside, along with seven known compounds. All the compounds were characterized by NMR including 2D NMR techniques. The isolates were evaluated for NF-κB, nitric oxide (NO), aromatase and QR2 chemoprevention activities and some of them appeared to be modestly active.  相似文献   

Spatial and temporal changes in chlorophyll fluorescence images of Nicotiana benthamiana leaves following inoculation with Pseudomonas syringae pv. tabaci     

M. J. Iqbal  P. H. Goodwin  E. D. Leonardos  B. Grodzinski 《Plant pathology》2012,61(6):1052-1062

Inoculation of Nicotiana benthamiana leaves with virulent and avirulent strains of Pseudomonas syringae pv. tabaci resulted in increasing changes in F_v/F_m, and NPQ over time. Images of these chlorophyll a fluorescence measurements revealed different changes in different zones of the leaf. For the virulent strain, the infiltrated zone and zone directly surrounding it showed decreased F_v/F_m, and NPQ before the appearance of visible symptoms, and these decreases corresponded with increasing bacterial populations and putative tabtoxin activity. Another distinct zone of reduced F_v/F_m and NPQ extended several centimetres from the lesion to the nearest leaf margin, but only very low bacterial populations and no putative tabtoxin activity were detected in this zone. For the avirulent strain, a hypersensitive response occurred, bacterial populations remained low, and there was little detectable putative tabtoxin activity. Decreased F_v/F_m and NPQ, but not , were observed in the infiltrated zone prior to the hypersensitive response, followed by decreased values in a zone directly surrounding it. Following that, no further changes were observed. These results demonstrate that in addition to detecting pre‐symptomatic impacts of bacteria, chlorophyll a fluorescence imaging can also show that there are highly distinct regions of affected tissue that can extend considerably beyond the area of bacterial colonization. This should be considered in selecting leaf tissues for examining the effects of pathogens on plants, such as altered host gene expression or protein levels.  相似文献   

Development of a pooled probe method for locating small gene families in a physical map of soybean using stress related paralogues and a BAC minimum tile path     

Kay L Shopinski  Muhammad J Iqbal  Jeffry L Shultz  Dheepakkumaran Jayaraman  David A Lightfoot 《Plant methods》2006,2(1):20-13

Background  

Genome analysis of soybean (Glycine max L.) has been complicated by its paleo-autopolyploid nature and conserved homeologous regions. Landmarks of expressed sequence tags (ESTs) located within a minimum tile path (MTP) of contiguous (contig) bacterial artificial chromosome (BAC) clones or radiation hybrid set can identify stress and defense related gene rich regions in the genome. A physical map of about 2,800 contigs and MTPs of 8,064 BAC clones encompass the soybean genome. That genome is being sequenced by whole genome shotgun methods so that reliable estimates of gene family size and gene locations will provide a useful tool for finishing. The aims here were to develop methods to anchor plant defense- and stress-related gene paralogues on the MTP derived from the soybean physical map, to identify gene rich regions and to correlate those with QTL for disease resistance.  相似文献   

Equid herpesvirus 1 is neurotropic in mice,but latency from which infectious virus can be reactivated does not occur     

Iqbal J  Edington N 《Acta veterinaria Hungarica》2002,50(1):117-129

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Growth and Physiological Responses of Quinoa to Drought and Temperature Stress          下载免费PDF全文

A. Yang  S. S. Akhtar  M. Amjad  S. Iqbal  S.‐E. Jacobsen 《Journal of Agronomy and Crop Science》2016,202(6):445-453

Quinoa (Chenopodium quinoa Willd.), traditionally called the mother of grains, has the potential to grow under high temperatures and drought, tolerating levels regarded as stresses in other crop species. A pot experiment was conducted in a climate chamber to investigate the potential of quinoa tolerance to increasing drought and temperature. Quinoa plants were subjected to three irrigation and two temperature regimes. At low temperature, the day/night climate chamber temperature was maintained at 18/8 °C and 25/20 °C for high temperature throughout the treatment period. The irrigation treatments were full irrigation (FI), deficit irrigation (DI) and alternate root‐zone drying (ARD). FI plants were irrigated daily to the level of the pot's water‐holding capacity. In DI and ARD, 70 % water of FI was applied either to the whole pot or to one side of the pot alternating, respectively. The results indicated that plant height and shoot dry weight significantly decreased by ARD and DI compared to FI treatment both at low and at high temperatures. However, plants in ARD treatment showed significantly higher plant height and shoot dry weight compared to DI especially at higher temperature, which is linked to increased xylem ion content. Higher quinoa plant growth in ARD was associated with increase in water‐use efficiency (WUE_i) due to higher abscisic acid concentration and higher nutrient contents compared to DI. From results, it can be concluded that quinoa plant growth is favoured by high temperature (25/20 °C) and ARD is an effective irrigation strategy to increase WUE in drought prone areas.  相似文献   

Genetic Variation for Salinity Tolerance in Pakistani Rice (Oryza sativa L.) Germplasm          下载免费PDF全文

A. Sakina  I. Ahmed  A. Shahzad  M. Iqbal  M. Asif 《Journal of Agronomy and Crop Science》2016,202(1):25-36

Soil salinity is one of the major production constraints. Development and planting of salt‐tolerant varieties can reduce yield losses due to salinity. We screened 185 rice genotypes at germination stage in petri dishes under control, 50, 100 and 150 mm salt stress, and at seedling stage in Yoshida's hydroponic nutrient solution under control, 50 and 100 mm salt stress. At germination stage, 15 genotypes including Nona Bokra, Sonahri Kangni, 7421, 7423 and 7467, whereas at seedling stage, 28 genotypes including Nona Bokra, Jajai‐77, KSK‐133, KSK‐282, Fakhr‐e‐Malakand, Pakhal, IR‐6, Khushboo‐95, Shahkar and Shua‐92 were found salt tolerant. Basmati‐370, Mushkan, Homo‐46 and accessions 7436, 7437 and 7720 were sensitive to salinity at both germination and seedling stage. We further screened a subset of 33 salt‐tolerant and salt‐sensitive genotypes with SSR markers. Four SSR markers (RM19, RM171, RM172 and RM189) showed significant association with two or more of the studied traits under 50, 100 and 150 mm salt stress. These markers may be further tested for their potential in marker‐assisted selection. The salt‐tolerant genotypes identified in this study may prove useful in the development of salt‐tolerant rice varieties in adapted genetic background.  相似文献   

Effect of ionic liquid on thermo-physical properties of bamboo biomass     

Nawshad Muhammad  Yanan Gao  Muhammad Irfan Khan  Zakir Khan  Abdur Rahim  Farasat Iqbal  Amir Sada khan  Jibran Iqbal 《Wood Science and Technology》2015,49(5):897-913

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Adjuvant effect of an extract from Cochinchina momordica seeds on the immune responses to ovalbumin in mice     

Chenwen Xiao  Songhua Hu  Zahid Iqbal Rajput 《Frontiers of Agriculture in China》2007,1(1):90-95

The seed of the Momordica cochinchinensis (Lour.) Spreng as a traditional Chinese medicine has been utilized in China for more than 1 200 years. It is traditionally used for the treatment of inflammatory swelling, scrofula, tinea, diarrhea as well as suppurative skin infections such as sore, carbuncles, furuncles and boils in both humans and animals. In this study, an extract from Cochinchina momordica seeds (ECMS) is evaluated for its adjuvant effect and safety. The results suggest that when co-administered with ECMS in Balb/c mice, ovalbumin (OVA, 10 μg) may induce significantly higher specific antibody production than OVA used alone (P < 0.05). Analysis of antibody isotypes indicates that the ECMS can promote the production of both IgG₁ and IgG_2a, but favor the IgG_2a. Splenocyte proliferative responses to concanavalin A, lipopolysaccharides or OVA are significantly higher in mice immunized with OVA mixed with ECMS than immunized with OVA alone or mixed with aluminum hydroxide (P < 0.05). No local reactions and negative effects on the body weight gain occurred after the injection of OVA mixed with various amounts of ECMS in mice. Therefore, the ECMS is safe for injection and can be used as a potential vaccine adjuvant biasing the production of IgG_2a in mice.  相似文献   

Bovine brucellosis – a comprehensive review     

Sandip Kumar Khurana  Anju Sehrawat  Ruchi Tiwari  Minakshi Prasad  Baldev Gulati  Muhammad Zubair Shabbir  Rajesh Chhabra  Kumaragurubaran Karthik  Shailesh Kumar Patel  Mamta Pathak  Mohd. Iqbal Yatoo  Vivek Kumar Gupta  Kuldeep Dhama  Ranjit Sah  Wanpen Chaicumpa 《The Veterinary quarterly》2021,41(1):61

Brucellosis is a zoonotic disease of great animal welfare and economic implications worldwide known since ancient times. The emergence of brucellosis in new areas as well as transmission of brucellosis from wild and domestic animals is of great significance in terms of new epidemiological dimensions. Brucellosis poses a major public health threat by the consumption of non-pasteurized milk and milk products produced by unhygienic dairy farms in endemic areas. Regular and meticulous surveillance is essentially required to determine the true picture of brucellosis especially in areas with continuous high prevalence. Additionally, international migration of humans, animals and trade of animal products has created a challenge for disease spread and diagnosis in non-endemic areas. Isolation and identification remain the gold standard test, which requires expertise. The advancement in diagnostic strategies coupled with screening of newly introduced animals is warranted to control the disease. Of note, the diagnostic value of miRNAs for appropriate detection of B. abortus infection has been shown. The most widely used vaccine strains to protect against Brucella infection and related abortions in cattle are strain 19 and RB51. Moreover, it is very important to note that no vaccine, which is highly protective, safe and effective is available either for bovines or human beings. Research results encourage the use of bacteriophage lysates in treatment of bovine brucellosis. One Health approach can aid in control of this disease, both in animals and man.  相似文献   

THE STATE OF POLLUTION LEVELS OF KARACHI HARBOUR AND ADJOINING COASTAL WATER     

Akhtar  Waseem  Ali  Iqbal  Zaidi  S. S. H.  Jilani  Seema 《Water, air, and soil pollution》1997,94(1-2):99-107

A study was carried out recently to determine the pollution level of waters in the Karachi harbour and adjoining backwaters. Nine locations were selected, four in the backwaters, two on the seaside, and three in the main navigable channel. Four of these locations were deliberately selected to coincide with those of a previous study conducted in 1982 by Pakistan Council of Scientific and Industrial Research (PCSIR) so that the values for the concentration of different pollutants could be compared. Analysis was conducted for pH, bicarbonates, Total Solids, Volatile matter in Total Solids, Chlorides, Sulphates, Calcium, Magnesium, Sodium and Potassium. The results indicate that the composition of sea water as far as the concentration of above constituents is concerned has not changed much since the time of the PCSIR study, viz 1982, except that the organic matter concentration has increased. The reasons for this increase in organic loading and its possible impact are discussed in this paper.  相似文献