Genomic DNA methylation of juvenile and mature Acacia mangium micropropagated in vitro with reference to leaf morphology as a phase change marker     

Baurens FC  Nicolleau J  Legavre T  Verdeil JL  Monteuuis O 《Tree physiology》2004,24(4):401-407

Genomic DNA methylation was analyzed in Acacia mangium Willd. microshoots micropropagated in vitro from juvenile and mature explants, and in relation to leaf morphology of the microshoots, which is considered a phase change indicator. Based on high performance liquid chromatography (HPLC) analyses, we found more DNA methylation in microshoots exhibiting juvenile leaf morphology (22.4%) than in microshoots of the mature phyllode morphological type (20.7%), irrespective of the age of the source material. Overall, the degree of DNA methylation in A. mangium microshoots was consistent with values reported for other angiosperms. Complementary investigations based on methylation sensitive amplification polymorphism (MSAP) techniques established that, of 1204 fragments revealed by the different primer pairs used, 49 (i.e., 4.08%) were derived from C(5m)CGG methylated sites. Three of these C(5m)CGG sites were exclusive to the juvenile plant material, and three sites were exclusive to the mature source. No fragments were associated specifically with leaf morphology, rather than with plant age. Thus, although the two age classes could not be distinguished based on a quantitative HPLC measure of DNA methylation, qualitative differences existed, as demonstrated by the six age-specific markers identified by MSAP. The reliability of the MSAP data was confirmed on a larger sample of juvenile plant material, which suggested that the total of six methylation markers detected is probably an underestimation of the age-related differences in DNA methylation that may exist between juvenile and mature plant materials.  相似文献   

Impact of low hydration of barley grain on β-glucan degradation and lipid transfer protein (LTP1) modifications during the malting process     

Runavot JL  Bakan B  Geneix N  Saulnier L  Moco K  Guillon F  Corbineau F  Boivin P  Marion D 《Journal of agricultural and food chemistry》2011,59(15):8256-8264

One of the objectives of the malting industry is to reduce the energy cost during kilning without major effect on malt quality. In this study, the impact of a low hydration steeping process on lipid transfer protein (LTP1) modifications and β-glucan breakdown was evaluated in low (LH) and high (HH) hydrated malts. LTP1 modifications analyzed by MS/MS revealed acylation, glycation, and disulfide bond breakage in both LH and HH malts. LTP1 free amine content measurement and fluorescence of Maillard protein adducts revealed no significant difference between LH and HH malts. Immunolabeling of LTP1 during malting highlighted the diffusion of the protein from the aleurone layer to the endosperm at the end of steeping in both LH and HH malts. By contrast, a significant higher amount of β-glucans was measured in LH malts after five days of germination, whereas no significant difference between LH and HH malts was revealed through immunostaining of β-glucans or evaluation of the endosperm integrity after seven days of germination. The possibility to reduce the effects of a low hydration steeping process on β-glucan hydrolysis by increasing germination time was discussed.  相似文献   

Disseminated Mycobacterium avium subspecies infection in a cat     

Rivière D  Pingret JL  Etievant M  Jechoux A  Lanore D  Raymond-Letron I  Boucraut-Baralon C 《Journal of Feline Medicine and Surgery》2011,13(2):125-128

An 18-month-old neutered male domestic shorthair cat, domiciled in the southwest of France, was first presented having suffered for a few days from dysorexia and vomiting. Abdominal palpation revealed lymph node enlargement. Cytological examinations of a fine needle aspirate demonstrated granulomatous inflammation with many non-staining elements consistent with mycobacteria. Diagnosis was confirmed by culture and polymerase chain reaction and Mycobacterium avium subspecies was isolated. Treatment was initiated with marbofloxacin, rifampicin and cefoxitin. There was a rapid clinical improvement. The cat suddenly died 2 months later. The main hypothesis is the administration of an inappropriate combination therapy that leads to the development of mycobacterial resistance. A volvulus and acute peritonitis secondary to the significant enlargement of a mesenteric lymph node were present at necropsy. Histopathological analysis of mesenteric lymph node, liver and spleen revealed multicentric granulomatous and severely necrotic lesions with numerous Ziehl-Neelsen positive intracytoplasmic elements.  相似文献   

Coastal environments shape chemical and microbial properties of forest litters in the Circum-Mediterranean region     

Amine Habib Borsali  Thomas Z. Lerch  Rania Besbes  Raphaël Gros  Isabelle Laffont-Schwob  Jean-Luc Boudenne  Fabio Ziarelli  Anne Pando  Anne Marie Farnet Da Silva 《European Journal of Soil Science》2021,72(2):1010-1025

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Electrochromic Properties and Electrochemical Behavior of Marennine,a Bioactive Blue-Green Pigment Produced by the Marine Diatom Haslea ostrearia     

Nellie Francezon  Mickaël Herbaut  Jean-Franois Bardeau  Charles Cougnon  William Blanger  Rjean Tremblay  Boris Jacquette  Jens Dittmer  Jean-Bernard Pouvreau  Jean-Luc Mouget  Pamela Pasetto 《Marine drugs》2021,19(4)

Marennine has long been known as the unique peculiar pigment responsible for the natural greening of oysters. It is specifically produced by the marine diatom Haslea ostrearia and it is a natural blue molecule indeed promising for food industry because of the rarity of such non-toxic, blue-colored pigments. In the search for its still not defined molecular structure, investigation of the color changes with the redox state has been carried out combining different approaches. Reducing and oxidizing chemicals have been added to purified marennine solutions and a stable blue-green color has been confirmed for the oxidized state, while a yellow color corresponded to the reduced unstable state. Raman spectroscopy has been used to monitor changes in the Raman spectra corresponding to the different colored states, and cyclic voltammetry has allowed the detection of a redox system in which protons and electrons are exchanged. These findings show that marennine is a suitable stable blue pigment for use in food applications and help in the elucidation of the chromophore structure.  相似文献   

Interstitial lung disease associated with Equine Infectious Anemia Virus infection in horses     

Pompei Bolfa  Marie Nolf  Jean-Luc Cadoré  Cornel Catoi  Fabienne Archer  Christine Dolmazon  Jean-Fran?ois Mornex  Caroline Leroux 《Veterinary research》2013,44(1):113

EIA (Equine Infectious Anemia) is a blood-borne disease primarily transmitted by haematophagous insects or needle punctures. Other routes of transmission have been poorly explored. We evaluated the potential of EIAV (Equine Infectious Anemia Virus) to induce pulmonary lesions in naturally infected equids. Lungs from 77 EIAV seropositive horses have been collected in Romania and France. Three types of lesions have been scored on paraffin-embedded lungs: lymphocyte infiltration, bronchiolar inflammation, and thickness of the alveolar septa. Expression of the p26 EIAV capsid (CA) protein has been evaluated by immunostaining. Compared to EIAV-negative horses, 52% of the EIAV-positive horses displayed a mild inflammation around the bronchioles, 22% had a moderate inflammation with inflammatory cells inside the wall and epithelial bronchiolar hyperplasia and 6.5% had a moderate to severe inflammation, with destruction of the bronchiolar epithelium and accumulation of smooth muscle cells within the pulmonary parenchyma. Changes in the thickness of the alveolar septa were also present. Expression of EIAV capsid has been evidenced in macrophages, endothelial as well as in alveolar and bronchiolar epithelial cells, as determined by their morphology and localization. To summarize, we found lesions of interstitial lung disease similar to that observed during other lentiviral infections such as FIV in cats, SRLV in sheep and goats or HIV in children. The presence of EIAV capsid in lung epithelial cells suggests that EIAV might be responsible for the broncho-interstitial damages observed.  相似文献   

Unexpected long-range edge-to-forest interior environmental gradients   总被引：1，自引：1，他引：0  

Laurent Bergès  Vincent Pellissier  Catherine Avon  Kris Verheyen  Jean-Luc Dupouey 《Landscape Ecology》2013,28(3):439-453

We examined the relationships between distance-to-edge and environmental factors inferred from mean plant indicator values across large distance-to-edge and patch size gradients. Floristic composition, landscape metrics and site variables (climate, soil and forest management) were collected on 19,989 plots in 1,801 forest patches in Northern France using the French National Forest Inventory. Statistical models were applied to mean plant indicator values (MIV) from Ellenberg and Ecoplant databases for soil pH, soil nitrogen (N), soil humidity (F), light (L) and air temperature (Ta) using distance-to-edge and forest patch size as predictors. The five mean indicator values significantly varied with distance-to-edge and MIV pH, N and Ta decreased over distances in excess of 500 m. Consistent very long edge-to-interior gradients were also detected for site variables. The distance-to-edge effect remained significant after controlling for site differences, especially for MIV pH and N. Significant edge-to-core gradients of MIV were detected over much larger ranges than previously recognised. Neither the presence of an ecological boundary between forest and the surrounding matrix, nor microclimate, soil or forest management heterogeneity within forest patches can fully explain this long edge-to-interior gradient observed in MIV. Two hypotheses are discussed for MIV pH and N: (1) soil eutrophication, due to atmospheric N deposition, which could occur deeper into forest-cores than previously acknowledged; (2) land use legacies, as the periphery of ancient forests is more often occupied by recent forests where former agricultural practices have irreversibly modified topsoil properties. Land use history data would help identify the drivers underlying these long-range edge gradients.  相似文献   

Cross-species reactivity of seven monoclonal antibodies with equine lymphocytes by flow cytometry     

Mérant C  Bonnefont C  Desbos A  Greenland T  Cadoré JL  Monier JC 《Veterinary research》2003,34(6):791-801

The recognition of equine lymphocyte antigens by monoclonal antibodies (mAbs) directed against human CD11a, CD18, CD21, CD23, CD29 and DR, as well as mouse CD23 was studied by flow cytometry. Unlike anti-CD11a, -CD21, -CD23 and DR mAbs, anti-CD18 and CD29 mAbs labelled the same percentage of horse peripheral blood lymphocytes (PBL) as human PBL. Double-staining with anti-horse immunoglobulin antibodies showed that anti-CD21 and -CD23 mAbs are mainly bound to peripheral blood B lymphocytes. The seven mAbs were also tested on the lymph node and thymus cells. The molecular targets of anti-CD11a, CD18 and CD29 mAbs were confirmed by immunoprecipitation of the membrane proteins. Our results suggest that anti-CD18, -CD29 and -DR mAbs recognise similarly expressed molecular homologues on equine cells, but that anti-CD11a, -CD21 and -CD23 mAbs recognise either different molecules or homologues that are expressed at different levels on horse cells.  相似文献   

"Candidatus Phlomobacter fragariae" Is the Prevalent Agent of Marginal Chlorosis of Strawberry in French Production Fields and Is Transmitted by the Planthopper Cixius wagneri (China)     

Danet JL  Foissac X  Zreik L  Salar P  Verdin E  Nourrisseau JG  Garnier M 《Phytopathology》2003,93(6):644-649

ABSTRACT Marginal chlorosis has affected strawberry production in France for about 15 years. A phloem-restricted uncultured bacterium, "Candidatus Phlomobacter fragariae," is associated with the disease. A large-scale survey for marginal chlorosis in French strawberry production fields and nurseries by polymerase chain reaction amplification of "Ca. P. fragariae" 16S rDNA revealed that symptoms of marginal chlorosis were not always induced by "Ca. P. fragariae" and that the stolbur phytoplasma could induce identical symptoms. "Ca. P. fragariae" was found to be predominant in strawberry production fields, whereas the stolbur phytoplasma was predominantly detected in nurseries. Two transmission periods of the disease, one in spring and the other from late summer to early fall, were evident. Cixius wagneri planthoppers captured on infected strawberry plants were demonstrated to be efficient vectors of "Ca. P. fragariae." The involvement in natural disease spread of the whitefly Trialeurodes vaporariorum, previously shown to acquire and multiply "Ca. P. fragariae" under greenhouse conditions, remains uncertain.  相似文献   

Evaluating the potential health gain of the World Health Organization's recommendation concerning vegetable and fruit consumption     

Hoffmann K  Boeing H  Volatier JL  Becker W 《Public health nutrition》2003,6(8):765-772

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