芝麻不同品种间超干种子耐藏性的差异   总被引：6，自引：0，他引：6  

弗洛朗  颜启传 《中国油料作物学报》2001,23(2):76-78

对17个不同芝麻品种超干种子经高温（50℃）老化处理14d后生活力和活力指数的变化情况进行了分析。结果表明，超干芝麻种子在32℃条件下贮藏56d后，种子仍能保持较高的生活力和活力。种子耐藏性的强弱有如下趋势：褐芝麻＞黑芝麻＞白芝麻＞黄芝麻。  相似文献   

Physiology and behavior of dogs during air transport   总被引：4，自引：0，他引：4       下载免费PDF全文

Rene Bergeron  Shannon L. Scott  Jean-Pierre mond  Florent Mercier  Nigel J. Cook    Al L. Schaefer 《Canadian journal of veterinary research》2002,66(3):211-216

Twenty-four beagles were used to measure physiological and behavioral reactions to air transport. Each of 3 groups of 4 sedated (with 0.5 mg/kg body weight of acepromazine maleate) and 4 non-sedated (control) dogs was flown on a separate flight between Montreal, Quebec, and Toronto, Ontario, after being transported by road from Quebec City to Montreal. Saliva and blood samples were taken before ground and air transport and after air transport. The heart rate was monitored during the whole experiment except during ground transport, and behavior was monitored by video during air transport. Sedation did not affect any of the variables measured. The mean plasma cortisol concentration was significantly higher (P < 0.05) after ground transport than at baseline (225.3 vs 134.5 nmol/L); the mean salivary cortisol concentration was significantly higher (P < 0.05) after both ground and air transport than at baseline (16.2 and 14.8, respectively, vs 12.6 nmol/L). The mean neutrophil count was significantly higher (P < 0.05) after both ground and air transport than at baseline (80.6 and 81.4, respectively, vs 69.5 per 100 white blood cells), whereas the mean lymphocyte count was significantly lower (P < 0.05) (13.2 and 13.7, respectively, vs 22.4 per 100 white blood cells). Loading and unloading procedures caused the largest increase in heart rate. On average, the dogs spent more than 50% of the time lying down, and they remained inactive for approximately 75% of the time, except during take-off. These results suggest that transportation is stressful for dogs and that sedation with acepromazine, at the dosage and timing used, does not affect the physiological and behavioral stress responses of dogs to air transport.  相似文献   

An IgM specific ELISA for the serodiagnosis of viral bovine respiratory infections     

G. Florent  A. Wiseman 《Comparative immunology, microbiology and infectious diseases》1990,13(4):203-208

A capture ELISA for the detection of IgM antibodies to Infectious Bovine Rhinotracheitis (IBR) and to Bovine Respiratory Syncytial (BRS) viruses was developed. In these assays, the first monoclonal antibody to bovine IgM is used as the catching antibody while the second monoclonal detects specific antiviral antibodies. The test was evaluated on serum samples originating from both experimentally and naturally infected animals. From these studies, it has been shown that primary IBR and BRS virus infections can be confirmed using serum samples collected 5–10 days after the appearance of the clinical signs of disease.  相似文献   

Enzyme linked immunosorbent assay used to monitor serum antibodies to bovine respiratory disease viruses     

G Florent  C de Marneffe 《Veterinary microbiology》1986,11(4):309-317

An enzyme linked immunosorbent assay (ELISA) was applied to the detection of serum antibodies against infectious bovine rhinotracheitis (IBR), parainfluenza-3 (PI3), adenovirus type 3 (adeno 3) and bovine respiratory syncytial (BRS) viruses. Paired serum samples from calves vaccinated with live attenuated virus vaccines were tested. The ELISA compared favorably with the virus neutralization test for detecting serologic responses to IBR, BRS, and adeno 3 viruses or with the hemagglutination inhibition test for PI3 virus. The simplicity, sensitivity and rapidity of the ELISA test makes it a useful tool for immunological studies with respiratory viruses.  相似文献   

Electrosurgical tenoscopic desmotomy of the accessory ligament of the superficial digital flexor muscle (proximal check ligament) in horses     

David F  Laverty S  Marcoux M  Szoke M  Celeste C 《Veterinary surgery : VS》2011,40(1):46-53

Objective: To report a tenoscopic technique using monopolar electrosurgery to transect the accessory ligament of superficial digital flexor muscle (AL‐SDFM) and outcome in 33 horses. Study Design: Case series. Animals: Horses (n=33). Methods: Medical files and surgery video recordings of horses that had AL‐SDFM desmotomy performed by tenoscopy with monopolar electrosurgical electrodes were reviewed. Results: Of 33 horses, 24 were Standardbred racehorses with surgery performed bilaterally for superficial digital flexor tendonitis and 9 horses had flexural deformity. Severe (n=6) and mild (6) intrathecal hemorrhage was the most common intraoperative complication. Large intrathecal vessels including the nutrient artery were successfully electrocoagulated and AL‐SDFM transection was completed. Clear/serosanguinous drainage from skin incisions was observed for 4.3±3.3 days (mean, SD). Protracted wound drainage for >4 days occurred in 10 horses, principally in the group treated for flexural deformities (P=.01). Conclusions: Sixty‐four AL‐SDFM were transected under tenoscopic observation using monopolar electrodes. Electrocoagulation of large intrathecal vessels, including the nutrient artery, was possible in all cases and allowed completion of desmotomy. Postoperative wound care was similar to routine tenoscopy in most (70%) horses. Aseptic protracted wound drainage was observed in 30% of horses (principally those with flexural deformity), and led to a prolonged hospitalization.  相似文献   

Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification     

Giuseppe Barraco  Isabelle Sylvestre  Giovanni Iapichino  Florent Engelmann 《Scientia Horticulturae》2011

In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3 M sucrose, then for 5 h in liquid medium with 0.7 M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20 min in a loading solution containing 1.9 M glycerol + 0.5 M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2 M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation.  相似文献   

Comparing encapsulation-dehydration and droplet-vitrification for cryopreservation of sugarcane (Saccharum spp.) shoot tips     

Giuseppe Barraco  Isabelle Sylvestre  Florent Engelmann 《Scientia Horticulturae》2011

In this study, in vitro shoot tips of two sugarcane clones were successfully cryopreserved using encapsulation-dehydration and droplet-vitrification with two vitrification solutions, PVS2 and PVS3. For both clones, encapsulation-dehydration induced significantly higher recovery, reaching 60% for clone H70-144 and 53% for clone CP68-1026, compared with droplet-vitrification in which recovery was 33–37% for clone H70-144 and 20–27% for clone CP68-1026. Optimal conditions included preculture of encapsulated shoot apices for 24 h in liquid medium with 0.75 M sucrose and dehydration with silica gel to 20% moisture content (fresh weight basis) before direct immersion in liquid nitrogen. With both protocols employed, regrowth of cryopreserved samples, as followed by visual observation, was always rapid and direct.  相似文献   

Efficacy of bithionol as an oral treatment for amoebic gill disease in Atlantic salmon Salmo salar (L.)     

Renee L. Florent  Joy A. Becker  Mark D. Powell 《Aquaculture (Amsterdam, Netherlands)》2007,270(1-4):15-22

This study examined the efficacy of bithionol as an oral treatment for Atlantic salmon Salmo salar affected by amoebic gill disease (AGD). The current commercial management strategy of AGD is a costly 3 h freshwater bath. It is labour intensive and the number of baths needed appears to be increasing; hence, there is an effort to identify alternative treatments. Efficacy was examined by feeding AGD-affected Atlantic salmon twice daily to satiation with bithionol, an antiprotozoal, at 25 mg kg^− 1 feed. Three seawater (35‰, 17 °C) re-circulation systems were used each consisting of three tanks containing 32 Atlantic salmon smolts with an average (± SEM) mass of 90.4 g (± 5.2). Three feeds were examined in the trial including bithionol, plain commercial control and oil coated commercial control. Feeding commenced 2 weeks prior to exposure to Neoparamoeba spp. at 300 cells L^− 1 and continued for 28 days post-exposure. Efficacy was determined by examining gross gill score and identifying percent lesioned gill filaments twice weekly for 4 weeks post-exposure. Bithionol when fed as a two-week prophylactic treatment at 25 mg kg^− 1 feed delayed the onset of AGD pathology and reduced the percent lesioned gill filaments by 53% and halved the gill score from 2 to 1 when compared with both the plain and oil controls during an experimental challenge. There were no palatability problems observed with mean feed intake of bithionol over the trial duration with fish consuming higher levels of the bithionol diet compared to both the oil and plain controls. This study demonstrated that bithionol at 25 mg kg^− 1 feed, when fed as a two-week prophylactic treatment for Neoparamoeba spp. exposure, delayed and reduced the intensity of AGD pathology and warrants further investigation as an alternative to the current freshwater bath treatment for AGD-affected Atlantic salmon.  相似文献   

Arthroscopic surgical approach and intra-articular anatomy of the equine suprapatellar pouch     

Vinardell T  David F  Morisset S 《Veterinary surgery : VS》2008,37(4):350-356

Objective— To describe a direct arthroscopic approach to the suprapatellar pouch (SPP), its intra-articular anatomy, and report our clinical experience with SPP arthroscopy.
Study Design— (1) Experimental study and (2) retrospective clinical study.
Animals— (1) Cadaveric equine hind limbs (n=24; 14 horses) and (2) 5 horses.
Methods— Four femoropatellar joints (FPJ) were dissected or injected with latex to document surface landmarks and topographic anatomy. (1) Arthroscopic exploration of 20 SPP (10 equine cadavers) was performed and described, followed by dissection. (2) Medical records of horses that had FPJ arthroscopy were reviewed for cases where SPP portals were required.
Results— (1) The optimal arthroscopic portal was located ∼10 cm lateral to the longitudinal patellar axis and 2 cm proximal to the patellar base in the intermuscular septum between the biceps femoris and vastus lateralis muscles. This approach allowed arthroscopic observation of the proximal aspect of the lateral and medial femoral trochlear ridges, the intertrochlear groove, the patellar base, and the synovial recess of the SPP. (2) Additional arthroscopic and instrument suprapatellar portals were used in 5 of 25 horses to complete lavage/debridement in osteochondritis dissecans (OCD) and septic arthritis.
Conclusion— Suprapatellar arthroscopy improved arthroscopic observation of structures located proximally in the FPJ and facilitated surgical access to the SPP.
Clinical Relevance— SPP arthroscopy is a safe and complementary approach to traditional distal FPJ arthroscopy that could be helpful for inspection and removal of free fragments/debris/fibrin located in the SPP, for OCD lesions extending proximally, and fracture(s) involving the proximal aspect of the patella.  相似文献   

Effects of syringe type and storage temperature on results of blood gas analysis in arterial blood of horses   总被引：1，自引：0，他引：1  

Picandet V  Jeanneret S  Lavoie JP 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(3):476-481

BACKGROUND: Results of arterial blood gas analysis can be biased by pre-analytical factors, such as time to analysis, syringe type, and temperature during storage. However, the acceptable delay between time of collection and analysis for equine arterial blood gas remains unknown. HYPOTHESIS: Dedicated plastic syringes provide better stability of arterial blood gases than multipurpose plastic syringes. ANIMALS: Eight mares, 1 stallion, and 1 gelding, ages 3 to 10 years old. METHODS: Arterial blood samples were collected in a glass syringe, a plastic syringe designated for blood gas collection, and a multipurpose tuberculin plastic syringe. Blood samples were stored at ambient temperature or in iced water. For each sample, partial pressure of oxygen in arterial blood (PaO2), partial pressure of carbon dioxide in arterial blood (PaCO2), and pH were measured within a few minutes of collection and at 5, 20, 30, 60, 90, and 120 minutes after collection. RESULTS: Collection into glass syringes stored in iced water provided adequate PaO2 results for up to 117 +/- 35 minutes, whereas blood collected in either of the plastic syringes resulted in a variation >10 mm Hg after 10 +/- 3 to 17 +/- 2 minutes, depending on the storage conditions. Plastic syringes kept at ambient temperature offered more stability for PaCO2 analysis because they could be stored up to 83 +/- 16 minutes without significant variations. Values of pH did not show variations more than 0.02 for the first hour, irrespectively of storage condition. CONCLUSIONS AND CLINICAL IMPORTANCE: Glass syringes placed on ice are preferable for analysis of PaO2. Blood collected in plastic syringes should be analyzed within 10 minutes, irrespective of the storage temperature, to ensure the accuracy of PaO2 values.  相似文献