Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology     

Chiara Gomiero  Giulia Bertolutti  Tiziana Martinello  Nathalie Van Bruaene  Sarah Y. Broeckx  Marco Patruno  Jan H. Spaas 《Veterinary research communications》2016,40(1):39-48

Tendons regenerate poorly due to a dense extracellular matrix and low cellularity. Cellular therapies aim to improve tendon repair using mesenchymal stem cells and tenocytes; however, a current limitation is the low proliferative potential of tenocytes in cases of severe trauma. The purpose of this study was to develop a method useful in veterinary medicine to improve the differentiation of Peripheral Blood equine mesenchymal stem cells (PB-MSCs) into tenocytes. PB-MSCs were used to study the effects of the addition of some growth factors (GFs) as TGFβ3 (transforming growth factor), EGF2 (Epidermal growth factor), bFGF2 (Fibroblast growth factor) and IGF-1 (insulin-like growth factor) in presence or without Low Level Laser Technology (LLLT) on the mRNA expression levels of genes important in the tenogenic induction as Early Growth Response Protein-1 (EGR1), Tenascin (TNC) and Decorin (DCN). The singular addition of GFs did not show any influence on the mRNA expression of tenogenic genes whereas the specific combinations that arrested cell proliferation in favour of differentiation were the following: bFGF2 + TGFβ3 and bFGF2 + TGFβ3 + LLLT. Indeed, the supplement of bFGF2 and TGFβ3 significantly upregulated the expression of Early Growth Response Protein-1 and Decorin, while the use of LLLT induced a significant increase of Tenascin C levels. In conclusion, the present study might furnish significant suggestions for developing an efficient approach for tenocyte induction since the external administration of bFGF2 and TGFβ3, along with LLLT, influences the differentiation of PB-MSCs towards the tenogenic fate.  相似文献   

Detection of novel polymorphisms in the ckit gene of canine patients with lymphoma,melanoma, haemangiosarcoma,and osteosarcoma     

Irina Gramer  Martin Kessler  Joachim Geyer 《Veterinary research communications》2016,40(2):89-95

  相似文献   

Immunological and pathological investigations in equine experimental uveitis     

Galina P. Simeonova  Svetozar Z. Krastev  Radostin S. Simeonov 《Veterinary research communications》2016,40(3-4):107-115

  相似文献   

非亲和性稻瘟病菌对水稻的诱导抗性     

王凡  朱财存  曹玉妍  贺闽  许靖波  邓华凤  邢俊杰 《植物保护学报》2023,50(4):905-912

为研究稻瘟病菌Magnaporthe oryzae不同菌株间的相互作用,选择与单抗性基因系水稻IRBL5-M （携带抗性基因Pi5）表现为亲和性的菌株HN52与非亲和性的菌株HN119为研究对象,将其单独或混合接种到单抗性基因系水稻IRBL5-M中,并通过荧光显微镜观察接种后水稻叶鞘的发病情况及病斑面积,测定接种后水稻内相关抗性基因OsWRKY45、OsNPR1、OsPR10、OsMAPK2的表达量以及活性氧的变化。结果显示,相较于单独接种亲和性菌株,混合接种后单抗性基因系水稻IRBL5-M病斑发病面积减少;混合接种中亲和性菌株HN52菌丝侵染能力降低,侵染菌丝细胞间扩展率显著降低73.13%;同时单抗性基因系水稻IRBL5-M中OsWRKY45、OsNPR1、OsPR10和OsMAPK2抗性基因表达量显著增加,水稻叶片中活性氧含量增加,表明在菌株混合侵染过程中,非亲和性菌株可通过激发水稻的抗性反应来降低亲和性菌株对水稻的侵染程度。  相似文献   

施用不同氮肥对盐碱地湖南稷子生产性能以及养分吸收的影响     

贾晓辉  张峰举  谢小伟  王斌  王腾飞  倪旺  陈月娟  许兴 《草地学报》2023,31(1):272-279

为探讨盐碱地湖南稷子(Echinochloa frumentacea)高效生产的最佳施氮种类和施氮量,采用单因素随机区组设计,通过在湖南稷子拔节期设置不施肥(CK)、尿素和硫酸铵(N1,N2)轻施肥(F1:50 kg·hm^-2)、中施肥(F2:100 kg·hm^-2)、重施肥处理(F3:150 kg·hm^-2),研究不同氮肥和施氮水平下湖南稷子田间农艺性状、养分吸收及氮肥利用率的变化,探讨盐碱地湖南稷子实现高效生产的适宜氮肥和施氮量。结果表明：与对照相比,施氮量为F2和F3时能够显著提高盐碱地湖南稷子产量及氮磷钾吸收量(P<0.05),植株不同部位氮含量上升,施氮量F3时能够显著提高氮肥利用效率(P<0.05),且同一施氮量下追施硫酸铵后湖南稷子产量、养分吸收及氮肥利用率均高于尿素。综上所述,氮肥追施量在150 kg·hm^-2时,有利于盐碱地湖南稷子的产量的提升和养分的吸收,且同一条件下追施硫酸铵较追施尿素效果显著。  相似文献   

猪源ST9型耐甲氧西林金黄色葡萄球菌中前噬菌体的流行状况与转导分析     

江南松  吉星  王亚新  孙城涛  汪洋  陈红梅  程龙飞  黄瑜  吴聪明 《畜牧兽医学报》2023,54(1):338-350

本研究旨在分析猪源ST9型耐甲氧西林金黄色葡萄球菌(Staphylococcus aureus,MRSA)中前噬菌体的流行情况、结构特点和转导能力,探究前噬菌体在猪源MRSA流行克隆形成中的作用。基于全基因组信息,分析了近年来从我国多省分离的131株ST9型MRSA中前噬菌体的流行率、分型、亲缘关系和结构特征;选取含不同分型前噬菌体的菌株进行诱导,对诱导获得的噬菌体颗粒进行转导,测定转导子的耐药表型及体外适应性。研究结果显示：猪源ST9型MRSA的前噬菌体携带率为78.6%(103/131株),其中,63株携带完整前噬菌体序列,所有前噬菌体序列均不含耐药基因,仅2.9%(3/103株)的前噬菌体序列含毒力基因;前噬菌体谱型丰富,其整合酶分型主要为Sa2int和Sa4int;各型别前噬菌体结构同源性较高,完整前噬菌体可被诱导为长尾噬菌体;噬菌体颗粒可包装供体菌的aadD、tet(L)耐药基因并转导至受体菌中;转导子可获得卡那霉素、四环素耐药表型,体外生长能力与受体菌株无明显差异(P>0.05)。研究结果表明：猪源ST9型MRSA的前噬菌体携带率较高,谱型丰富,不携带耐药基因,部分噬菌体可包装供体菌的耐药基因转导至受体菌,产生的适应性代价小。  相似文献   

植物表达载体pGMAR-BADH的构建   总被引：1，自引：0，他引：1  

朱永兴  许兴  陈受宜 《中国农学通报》2007,23(11):49-51

将甜菜碱醛脱氢酶基因BADH和植物表达载体pGMAR通过内切酶BamHI和KpnI双酶切,T4连接酶进行连接反应。重组质粒在大肠杆菌菌株DH5α内扩增,提取并纯化质粒。经鉴定,基因BADH已被完整、正确的插入到pGMAR载体中,并成功将BADH插入到载体pGMAR的两个MAR序列之间。  相似文献   

A Furry Synthetic Evaluation Model in Capital Structure Decisions     

TANG Ya li  LIU Xing 《保鲜与加工》2002,(4):91-94

This paper analyzes the limitation of regarding the weighted average cost of capital as the optimal standard of capital structure in the traditional capital structure decision-making method, and analyzes the view that the capital structure is affected by many factors,such as earning power, long-term stability of operating, the attitudes held by owners and operators, the attitudes held by creditors and financial risk. With the example, the affecting factors of the capital structure is analyzed. The fuzzy synthetic evaluation model are employed to decision-making of the capital structure. This article also shows the key point of its application.  相似文献   

Existence of an Arbitrage about Portfolio in an Unequilibrium Market     

FU Qiang  PU Xing cheng 《保鲜与加工》2001,(5):123-126

This article has discussed the problem of an arbitrage about an portfolio in an unequilimarket and got a determinable theorem about existence of an arbitrage about an portfolio. According to this theorem,an arbitrage about portfolio is not existing if there exists a respect martignal measure.A structural result about existence of arbitrage about an portfolio in a market is gotten. All results represent the existence of an arbitrage about an portfolio is related closely to the type of market.  相似文献   

A Caculation Method of Portfolio Investment under the Risk Mininiz     

Liu Xing  Yang Xiutai 《保鲜与加工》1995,(6):7-12

According to the security investment theory of Markowitz,this paper presents aCaculation method of portfolio investment under the risk minimization,and some mathematic expres-sions of the efficient frontier for portfolio investment are conducted.Based on these,the efficientfrontier is proved as a parabola,and a practical caculation process is given.  相似文献