NRF2 attenuates oxidative stress and lysosomal dysfunction in doxorubicin-induced H9C2 cells     

CHEN Fang  ZOU Lian-hong  LIU Xie-hong  YUAN Li-li  JIANG Yu 《园艺学报》2019,35(8):1359-1364

AIM:To study the effect of nuclear factor E2-related factor 2 (NRF2) on oxidative stress injury and lysosomal dysfunction in doxorubicin (DOX)-induced rat myocardial H9C2 cells. METHODS:The H9C2 cells were treated with DOX. The expression of NRF2 at mRNA and protein levels was determined by real-time PCR and Western blot. The H9C2 cells stably over-expressing NRF2 were established by lentiviral infection. Real-time PCR and Western blot were used to identify the efficiency of over-expression. After DOX treatment, the cell viability was measured by CCK-8 assay, the activity of lactate dehydrogenase (LDH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), and the content of malondialdehyde (MDA) in the cell supernatant were detected. FITC-dextran was used to analyze lysosomal pH, and the protein expression of lysosomal-associated membrane protein 1 (LAMP1) and cathepsin B was determined by Western blot.RESULTS:The expression of NRF2 at mRNA and protein levels in DOX-treated H9C2 cells was significantly decreased (P<0.05). Over-expression of NRF2 significantly up-regulated the mRNA and protein expression of NRF2 in DOX-treated H9C2 cells (P<0.05). After DOX treatment, the cell viability was decreased, and LDH activity was increased. The activity of SOD, GSH-Px and CAT was decreased, and the content of MDA was increased (P<0.05). The lysosomal pH was increased, and the protein expression of LAMP1 and cathepsin B decreased (P<0.05). Over-expression of NRF2 increased the cell viability, decreased LDH activity, increased the activity of SOD, GSH-Px and CAT, and decreased the content of MDA in cell supernatant (P<0.05). Over-expression of NRF2 also decreased the lysosomal pH, and increased the protein expression of LAMP1 and cathepsin B (P<0.05). CONCLUSION:DOX inhibits the expression of NRF2 in the myocardial H9C2 cells. Over-expression of NRF2 attenuates oxidative stress and lysosomal dysfunction in the H9C2 cells induced by DOX.  相似文献   

Effect of Jieyuwan on depression-like behaviors and expression of BDNF in hippocampus and prefrontal cortex of WKY rats     

SONG Miao  LIU Hong-mei  LI Qi  ZHANG Yu  XU Yong 《园艺学报》2019,35(6):1124-1129

AIM:To investigate the mechanism of depression and its development, and to study the expression of brain-derived neurotrophic factor (BDNF) in the hippocampus and prefrontal cortex of Wistar-Kyoto (WKY) rats treated with Jieyuwan. METHODS:Adult male WKY rats were used as an animal model of endogenous depression. Wistar rats of the same strain were selected as control group. WKY rats were randomly divided into model group, citalopram group and Jieyuwan group. After intragastric administration for 21 d, the changes of depression-like behaviors were observed by sucrose preference test and forced swimming test. Immunofluorescence and Western blot were used to detect the expression of BDNF in the hippocampus and prefrontal cortex. RESULTS:WKY rats showed significant depression-like behaviors, and the expression of BDNF was significantly decreased in the hippocampus and prefrontal cortex (P<0.01). The reduction of neuronal axons in hippocampus was also observed. After drug treatment, the depression-like behaviors of WKY rats were significantly attenuated, and the expression of BDNF and the number of axons were increased (P<0.01). CONCLUSION:Jieyuwan effectively attenuates the depression-like behaviors of WKY rats, and BDNF is a key factor in its antidepressant effect. Our findings further confirm the involvement of BDNF in the development of depression.  相似文献   

百香果品种‘紫香1号’果实糖、酸和维生素成分分析   总被引：1，自引：0，他引：1  

袁启凤  严佳文  王红林  李仕品  陈楠  王宇  韩秀梅  马玉华 《中国果树》2019,(4):43-47

为深入了解‘紫香1号’百香果成熟果实中的可溶性糖、有机酸和维生素组分及含量,采用高效液相色谱法测定各组分的含量。结果表明:‘紫香1号’百香果中的可溶性糖主要为葡萄糖、果糖和蔗糖,各糖分之间差异显著,其中蔗糖含量最高,达38.52mg/g,属蔗糖积累型;有机酸包括苹果酸、丙二酸、乳酸、柠檬酸和琥珀酸,各有机酸之间差异显著,以柠檬酸含量最高,达0.995 mg/g,属柠檬酸优势型;维生素包括维生素A、维生素B1、维生素C和维生素E,各种维生素之间差异达显著水平,其中维生素E含量最高,为430.61 mg/kg。可见,‘紫香1号’百香果含有丰富的可溶性糖、有机酸和维生素,具有较高的甜度和酸度值,故果实成熟时鲜食口感酸甜,风味浓郁。  相似文献   

两段式花生摘果特性试验研究   总被引：1，自引：0，他引：1  

周德欢  胡志超  曹明珠  王冰  王申莹  于昭洋 《农机化研究》2017,(6):183-187

为探明两段式收获条件下花生摘果特性,提高花生联合收获机的收获质量,以江苏宿迁地区天府9号为试验对象,应用室内与田间试验相结合的研究方法,研究收获期影响摘果特性的主要因素,揭示花生荚果含水率、晾晒时间、果柄力学特性和摘果作业质量的内在关系。结果表明:基于不同的试验条件与方法,果柄拉断力有所差异,但随晾晒时间(含水率)变化趋势一致,在晾晒2~3天时,果柄拉断力达到峰值,且秧柄节点拉断力大于果柄节点;确定最佳摘果时间为晾晒5~6天,并经捡拾联合收获机摘果试验验证。研究结果为摘果装置的结构参数和运动参数提供了直接依据,并为两段式收获模式下最佳捡拾摘果时间提供了参考,同时为深入研究摘果效率与摘果损失提供了参考。  相似文献   

我国农用无人机发展概况与展望   总被引：4，自引：0，他引：4  

杨陆强  果霖  朱加繁  高志超  周体全  喻自荣  彭继文  张汝坤 《农机化研究》2017,(8):6-11

近年来,随着我国农业结构的转变与调整,农业生产对农业机械的需要也从普遍的耕作机械、收获机械、农副产品加工机械等方面扩展到了农用无人机。无人机在有动力源的情况下,能通过操控来执行多种作业任务,可广泛应用于农业生产,主要用途体现在植保作业、林业监测、作物授粉及牧群定位等方面。为此,介绍了农用无人机的分类、国内外农用无人机的技术发展概况和现状,以及其在农业领域的应用,阐明了其在我国的发展前景,指出了其在发展过程中存在的一些问题,并提出了相应的发展建议。  相似文献   

捆草机电气控制系统的设计研究——基于MCGS与单片机技术     

邢小琛  武佩  刘宇  宣传宗  马彦华 《农机化研究》2017,(6)

以呼伦贝尔市蒙拓农机科技股份有限公司生产的9YG-1.3型圆草捆打捆机为样机,针对其控制部分存在自动化程度低、草捆边缘布网太松、操作繁琐及需人工手动计数等问题,运用昆仑通态MCGS编程软件和Keil u Vision4软件,设计了一款以单片机系统为控制核心,以MCGS触摸屏为人机界面,配备各种相应传感器和控制输出硬件的自动控制系统。该系统通过人机监控界面,实现了对捆草机运行状况的实时监控。试验结果表明:本控制系统有效地解决了捆草机的灵活布网问题与计数问题,并可以实时进行停车、开仓门、关仓门与报警声亮提示,提高了捆草机的工作效率和自动化水平。  相似文献   

D形截面螺旋输送机输送颗粒物料的实验研究   总被引：2，自引：0，他引：2  

刘春飞  李艳洁  王玉 《农机化研究》2017,(8):141-146

在可控转速的情况下运输粉末或者颗粒等松散物料时,因其机构简单,螺旋输送机被广泛应用于林业、农业等多种背景下的短距离提升或水平运输物料。为此,采用实验方法,针对改进后的D形截面筒的输送机进行输送颗粒物料的研究,结果表明:输送的质量流速率随着输送转速的增加而增加,随输送角度的增大而降低。另外,螺距和颗粒物料的粒径也对质量流速率有影响:大螺距的输送质量流速率较大,且随着输送倾斜角度的增大,螺距对质量流速率的影响减小;大颗粒的质量流速率大于小颗粒,且随着输送倾斜角度增加差距增大。  相似文献   

探析发动机气缸套磨损的原因及预防措施     

郑荻  杨自斌  徐昱 《湖南农机》2017,44(4)

气缸套作为发动机气缸的重要组成部分,在工作过程中的快速磨损,将会影响到发动机的正常寿命.文章阐述了引起气缸套磨损的三种情况:结构原因、使用不当、维修操作不当.在此基础上,提出了预防减少气缸套磨损的措施:正确起动发动机;正确选用润滑油;加强滤清器的保养;保持发动机正常工作温度.  相似文献   

弹齿滚筒式捡拾装置弹齿的静力学分析     

郁志宏  吴淑红  王文明  张建超 《农机化研究》2017,(4):27-31

针对弹齿滚筒式捡拾装置在工作过程中弹齿变形现象,对弹齿滚筒式捡拾装置弹齿进行静力学分析,以寻求解决的途径。通过对捡拾装置弹齿在3个工作阶段上的载荷进行分析后,获得各工作阶段弹齿的受力简图,通过比较判断出在捡拾升运阶段弹齿受力比较复杂。以9KJ-1.4A型压捆机捡拾装置为实例,经过计算在有随机载荷和无随机载荷时弹齿齿臂末端的应力情况,得出结论:在没有随机载荷的情况下,齿根处应力远小于其许用应力,弹齿产生弹性变形,有足够的强度;当有随机载荷时,对弹齿产生冲击,若随机载荷过大,垂直于弹齿轴向的分力大于24N,则弹齿会产生塑性变形。对捡拾装置进行静力分析,可为捡拾装置整体结构优化设计供理论依据。  相似文献   

压缩生物质颗粒旋流燃烧炉结构设计与数值模拟     

刘庆刚  张朝  于新奇  李洪涛 《农机化研究》2017,(12):253-257

针对目前生物质燃烧炉燃烧效果和设备成本的之间的矛盾,在压缩生物质颗粒燃烧炉的设计中引入旋流燃烧技术,设计了一种压缩生物质颗粒旋流燃烧炉,并采用Fluent软件对设计的压缩生物质颗粒旋流燃烧炉的性能进行了数值模拟。研究结果表明:设计的压缩生物质颗粒旋流燃烧炉的性能能够满足小型工业企业和家庭用户的需求,同时能够有效降低烟气中CO的含量,烟气中CO含量远远低于国家标准要求。研究结果对改进生物质燃烧炉结构、提高生物质燃烧炉性能等方面具有一定的意义。  相似文献