Characterization of the quantitatively major collagen in the mantle of oyster <Emphasis Type="Italic">Crassostrea gigas</Emphasis>     

Shoshi?MizutaEmail author  Tomoyuki?Miyagi  Reiji?Yoshinaka 《Fisheries Science》2005,71(1):229-235

ABSTRACT:   A quantitatively major collagen was isolated from the pepsin-solubilized collagen preparation of the mantle by differential salt precipitation and phosphocellulose column chromatography, and its constituent α components (α1 and α2) were purified by phosphocellulose column chromatography. The subunits were demonstrated to be genetically distinct from each other by peptide mapping and amino acid analysis. The amino acid composition calculated from those of the α1 and α2 components in 2 : 1 ratio coincided well with that of the major collagen from the mantle. These results suggest that the major collagen in the mantle of the oyster may have a heterotrimer structure (α1)₂α2.  相似文献   

Effects of additives on the thermal gelation of Japanese common squid natural actomyosin     

Eiji?Tsujioka  Tsukasa?Ehara  Nobuyuki?Kanzawa  Satoshi?Noguchi  Takahide?TsuchiyaEmail author 《Fisheries Science》2005,71(3):688-690

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Evaluation of coral bleaching condition <Emphasis Type="Italic">in situ</Emphasis> using an underwater pulse amplitude modulated fluorometer   总被引：3，自引：0，他引：3  

Mineo?OkamotoEmail author  Satoshi?Nojima  Yasuo?Furushima  Hajime?Nojima 《Fisheries Science》2005,71(4):847-854

ABSTRACT:   Photosynthetic activities of hermatypic corals (28 genera, c . 68 species and c. 92 specimen) were measured in November 2000 at Sekisei lagoon, Okinawa, Japan, using an underwater pulse amplitude modulated fluorometer. Maximum quantum yield (Fv/Fm) ranged from 0.61 to 0.70 and had an average value of 0.664 (standard deviation [SD], 0.0289). Following the measurement of Fv/Fm, quantum yield (Fm'-F)/Fm' at eight-leveled actinic-light intensities were obtained from all specimens. Values of electronic transport rates (ETR) were nearly the same under low photosynthetic active radiation (PAR; µmol quanta/m² per s) condition and varied with increasing PAR. The value of ETR at 100 µmol quanta/m² per s ranged from 21.3 to 30.0 and had an average of 27.8 (SD, 2.09). In August 2001, a coral bleaching event was observed in Sekisei Lagoon. During the event, photosynthetic activities of 21 specimens of eight species (seven genera) were measured. By applying two indicators, Fv/Fm and ETR at 100 µmol quanta/m² per s obtained in August 2000, 18 of 21 specimens were suggested under the bleaching process. In case of Porites lutea , seven colonies were visually evaluated for their condition of bleaching and were found to have two 'normal', three 'slightly bleached' and two 'heavily bleached'. From the density of zooxanthellae, colonies evaluated 'normal' or 'slightly bleached' did not show an apparent difference. However, they were clearly identified by Fm/Fv and ETR at 100 µmol quanta/m² per s.  相似文献   

Changes in rhodopsin-porphyropsin ratio of chum and pink salmon     

Ei-ichi?HasegawaEmail author 《Fisheries Science》2005,71(5):1091-1097

ABSTRACT:   In order to investigate the changes in rhodopsin-porphyropsin ratio of chum salmon and pink salmon in relation to the change in their habitat, the ratios were analyzed in individual fish prior to stocking, during the sea run, homing, and upstream migration. The ratio in both the species gradually increased during the sea run. However, the ratio decreased after upstream river migration. Moreover, in the sea, the rhodopsin-porphyropsin ratio of chum salmon was always slightly higher than that of pink salmon. The largest difference in the individual variation was observed in the individuals caught with a set net placed near the mouth of a home river.  相似文献   

Effect of a seaweed mixture on serum lipid level and platelet aggregation in rats     

Hideomi?AmanoEmail author  Makoto?Kakinuma  Daniel?A?Coury  Haruka?Ohno  Takaaki?Hara 《Fisheries Science》2005,71(5):1160-1166

ABSTRACT:   To assess the effect of a seaweed mixture on lipid levels in serum as well as platelet aggregation in rats, Eisenia bicyclis ('Arame'), Hizikia fusiformis ('Hijiki') and Undaria pinnatifida sporophylls ('Mekabu'), all brown seaweeds, and Porphyra yezoensis ('Susabinori'), a red seaweed, were powdered and mixed in a ratio of 45:30:20:5 (w/w). When rats were fed a cholesterol-rich diet containing this mixture of seaweeds (9–10% w/w) for 28 days, serum total cholesterol, LDL-cholesterol, free cholesterol, and triglyceride levels declined significantly to 49.7%, 48.1%, 49.0% and 74.8%, respectively, of those of the control. Serum HDL-cholesterol, however, was unchanged. Though activated partial thromboplatin time, prothrombin time, antithrombin III activity, and fibrinogen levels in plasma were unchanged, the maximal ADP- and collagen-induced platelet aggregation decreased significantly to 89.0% and 85.5% control levels, respectively. These results indicate that this mixture of E. bicyclis , H. fusiformis , U. pinnatifida sporophylls, and P. yezoensis , is useful for the prevention of hyperlipidemia and thrombosis in rats.  相似文献   

Trypsin restoration time in the pyloric ceca of yellowtail <Emphasis Type="Italic">Seriola quinqueradiata</Emphasis>     

Koji?Murashita  Satoshi?Kubota  Patricia?Yumi?Morimoto Kofuji  Hidetsuyo?Hosokawa  Toshiro?MasumotoEmail author 《Fisheries Science》2005,71(6):1274-1279

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Keeping of milking cattle from economic and genetic aspects     

H Kr?usslich 《Tier?rztliche Praxis》1973,1(1):413-415

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Sequence and expression analysis of cathepsin S gene in the miiuy croaker <Emphasis Type="Italic">Miichthys miiuy</Emphasis>     

Sun YN  Xu TJ  Wang JX  Cheng YZ  Wang RX 《Fish physiology and biochemistry》2011,37(4):761-765

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Evaluation of VI-alpha and PIT-tagging of the seahorse <Emphasis Type="Italic">Hippocampus abdominalis</Emphasis>     

Chris?M.?C.?WoodsEmail author 《Aquaculture International》2005,13(3):175-186

The suitability of visible implant alphanumeric (VI-alpha) and passive integrated transponder (PIT)-tagging to individually identify seahorses (Hippocampus abdominalis) was assessed in two trials. For each trial, 24 seahorses were tagged and mortality, growth, tag retention and tag visibility/readability assessed, together with 24 control seahorses, over a period of 3 months. For VI-alpha tagging, a single tag was inserted under the skin between the first two anterior lateral tail rings of the seahorses. There was no difference in final seahorse length, wet weight, or mean SGR in tagged seahorses, compared with control seahorses, after 3 months. Tag retention was 100%, as was survival, in both treatments. Tag detection with the naked eye was generally poor but improved using LED blue light. However, readability of tag codes was highly variable and insufficiently reliable for VI-alpha to be suitable for identification of individual seahorses. In PIT-tagged seahorses, a single FDX-B transponder was inserted into the abdominal cavity of seahorses. There was also no difference in final seahorse length, wet weight, or mean SGR, compared with control seahorses, after 3 months. Tag retention was also 100%, as was survival in both treatments. Readability of transponders was reliable and quick using a compact reader. PIT-tagging is considered suitable for individual identification of large H. abdominalis.  相似文献   

Movement of the fluvial form of masu salmon, <Emphasis Type="Italic">Oncorhynchus masou masou</Emphasis>, in a mountain stream in Kyushu,Japan     

Kazuhiro?SakataEmail author  Takuya?Kondou  Naohiko?Takeshita  Akinobu?Nakazono  Seir??Kimura 《Fisheries Science》2005,71(2):333-341

Using mark-recapture methods, the movements of the fluvial form of masu salmon (Oncorhynchus masou masou) in a mountain stream on the island of Kyushu, Japan, were studied. Most (78%) of the masu salmon were recaptured in the pool in which they had been originally caught and tagged. Of those that moved between pools, the proportion of individuals that moved during the breeding period was not significantly higher than the proportion that moved during the non-breeding period. However, during the breeding period, a higher proportion of larger salmon moved than did smaller fish. The proportion of mobile large males during breeding period was higher than that for small males. Also, it was found that a few individuals showed long-range movement in the autumn. As a long-term movement, 78 individual fish (65%) that were recaptured more than three times showed high sedentary tendencies. Sixteen individual mobile fish (13%) moved and returned to the original pool. Fluvial form of masu salmon in Kyushu show a high sedentary nature; however, large mature males seem to actively move in search of female during breeding period.  相似文献