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61.
The male sterility fluctuation of photo-thermo-sensitive genic ma1e sterile〔P(T) GMS〕lines induced by low air temperature is a key problem troubling the application of two-line system hybrid rice. The paper suggests to solve this problem by the breeding approeches, i.e, developing low sterile critical temperature P(T)GMS lines, herbicie resistant restorer lines, herbicide sensitive P(T)GMS lines and tetracycline-conditioned P(T)GMS lines to ensure the hybrid purity; and by the hybrid seed production techniques, i.e, taking the correct procedure of foundation seed production, the suitable district and season for hybrid seed production and the deep irrigation method against cold weather in high summer. 相似文献
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我国小型渔船(船长20~35米)捕鱼作业信号灯至今未能达到《1972年国际海上避碰规则(1989年修订本)》的要求。本文根据光学原理,对号灯间距分辨率进行了多次测试和研究,并根据我国渔船客观实际提出了小型渔船号灯设置最佳配置方案。 相似文献
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A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献
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番茄不同部位中糖含量和相关酶活性的研究 总被引:25,自引:2,他引:25
试验将番茄光合产物运转途径上叶片(源) 、运输系统以及果实(库) 区分开, 分别测定其糖的组成和含量以及糖代谢相关酶的活性。结果表明: 番茄光合产物运转途径上从“源”到“库”各部位糖的组成和含量不同。叶肉中果糖的含量最高, 蔗糖的含量最低; 中筋中以果糖和葡萄糖为主; 叶柄维管束中葡萄糖含量最高, 蔗糖含量次之, 果糖含量最低。节间和果柄维管束中主要含有蔗糖。果实维管束以及果实内各部位中则主要含有葡萄糖和果糖, 且两者含量无显著差异, 蔗糖含量很低。萼片中葡萄糖含量最高, 蔗糖含量最低; 果蒂中3种糖含量均较高且无显著差异。番茄叶肉及光合产物运转组织中转化酶活性很低, 而在库器官的非维管组织中转化酶活性较高。果蒂中的蔗糖合成酶( SS) 活性最高, 其次是叶肉和运转组织, 果实内各部位中SS活性较低。在合成蔗糖的器官—叶肉中, 有较高的蔗糖磷酸合成酶( SPS) 活性, 运转组织中的SPS活性较叶肉中降低, 但果柄维管束和果实维管束中则表现出较高SPS活性, 果肉、果胶质胎座及心室隔壁中的SPS活性最低。 相似文献
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