玉米秸秆芯结构建模与径向压缩过程模拟   总被引：2，自引：1，他引：1  

廖娜  黄光群  陈龙健  贺城  韩鲁佳 《农业机械学报》2011,42(6):117-121

为了深入研究实芯作物秸秆的压缩特性,以农大108玉米秸秆芯为研究对象,基于秸秆各向异性、非均质、非线性特点,先通过径向压缩试验获取其粘弹性参数,然后应用ANSYS软件建立了玉米秸秆芯横截面圆柱有限元结构模型,并进行径向压缩过程和应力松弛过程的数值模拟.研究表明,五参量广义麦克斯韦模型能较好地描述玉米秸秆芯应力松弛特性,模型拟合参数的试验值与有限元模拟值误差均小于10％.试验验证了玉米秸秆芯模拟结果的可行性和计算模型的有效性.  相似文献   

超声聚焦微喷制备脂质体微阵列研究     

韩鑫  张波  李笋  张德远 《农业机械学报》2011,42(11):231-234

设计了基于超声换能器和球面声透镜的超声聚焦微喷头及向下隔离喷射机构,实现了无喷嘴、无堵塞、非接触、低消耗、高精度的微阵列制备.为验证其生物相容性,以载有F0F1- ATP酶的分子马达脂质体为对象,制备出了形状规则、定位准确的脂质体微阵列,并对其ATP水解活性和合成活性进行了检测.实验结果表明,所制备的微阵列生物芯片在相关光化学刺激下具有良好的ATP水解与合成活性,从而证实该方法在微阵列生物芯片制备过程中能够较好地保持样品的生物活性.  相似文献   

玉米植株抗弯特性对分禾器结构的影响分析   总被引：3，自引：0，他引：3  

吴鸿欣  陈志  韩增德  郝付平  曹洪国  王高斌 《农业机械学报》2011,42(Z1):6-9

玉米收获机分禾器结构直接影响玉米植株的分禾和输送效果,是决定机具能否正常作业的重要因素。通过田间试验,对玉米植株抗弯特性进行了测定,测量出玉米植株的横向最大偏移量,并计算出玉米植株偏折角。得出了分禾器宽度与玉米植株抗弯特性之间的关系方程,找到了影响分禾输送效果的一个重要因素——植株自身的抗弯特性,为分禾器的设计提供了新的依据。在理论上解释了作业时分禾器离地越高越不易碰伤植株的现象,且对比了不同地区品种玉米植株抗弯强度的差异性,结论是最佳收获期内山东省、北京市地区品种的玉米植株抗弯能力比吉林省地区品种强。  相似文献   

螺旋齿辊式秸秆调质装置性能试验   总被引：2，自引：1，他引：1  

李文哲  董欣  王德福  韩增德  曹洪国  吴鸿欣 《农业机械学报》2011,42(12):143-147

利用所研制的螺旋齿辊式秸秆调质装置试验台,采用调质齿辊差速转动方式对摘穗后玉米秸秆进行压裂、破节的连续调质正交试验,分析了调质齿辊工作间隙、调质齿辊转速及秸秆喂入速度对秸秆调质性能的影响.试验结果表明,调质齿辊工作间隙对秸秆调质作业性能影响显著,最优参数组合为调质齿辊工作间隙2 mm,调质齿辊转速65 r/min,秸秆喂入速度4 km/h.  相似文献   

一个新的体外快速鉴定十字花科黑腐病菌Ⅲ型效应物的报告质粒的构建     

唐菲菲  韩恩兴  梁业瀛  刘三  姜伯乐 《广西农业生物科学》2011,30(5):626-631

植物病原菌侵染寄主的过程就是病原菌和寄主植物相互作用的过程。在这个相互作用过程中,Ⅲ型分泌系统和Ⅲ效应物与病原菌致病密切相关。大部分革兰氏阴性植物病原菌通过Ⅲ型分泌系统定向的把效应物蛋白传递到宿主细胞,效应物蛋白进入植物体引起致病或过敏反应。本研究将百日咳杆菌的腺苷酸环化酶基因连接至含启动子的pLAFRJ载体上,从而构建出一个新的体外快速鉴定Ⅲ型效应物的报告质粒pJ-JA,并用已鉴定为Ⅲ型效应物基因的十字花科黑腐病菌XC1553的启动子和信号区验证该报告质粒,证明这个系统是可以工作的。该报告质粒为进一步精确筛选鉴定十字花科黑腐病菌Ⅲ型效应物提供了材料。  相似文献   

浅析项目管理的发展历史与工作过程     

韩千兵 《农业装备与车辆工程》2011,(8):50-52,55

介绍了项目和项目管理的定义,阐述了项目管理的发展过程,详细论述了现代项目管理5大工作过程组和各工作过程组具体的过程范围及相互之间的作用。  相似文献   

固态发酵芦笋老茎生产高蛋白反刍动物饲料的研究     

王琳琳  韩建荣  李欣欣  李艳婷 《饲料工业》2011,32(21)

通过单菌种固态发酵、复合菌种固态发酵以及不同配比的复合菌种固态发酵试验,对能够将芦笋老茎转化为反刍动物饲料的微生物菌种进行了筛选。结果表明,由青霉(Penicillium sp.)F-5、暗孢毛壳(Chaetomium atrosporum)F-21、曲霉(Aspergillus sp.)F-25菌株组成的配比为2:2:3的复合菌种是效果最好的菌种组合。芦笋老茎生料培养基经该复合菌种5 d固态发酵后,发酵产物中可溶性蛋白含量达到4.80 mg/g,与对照组相比增加了39.94%;粗蛋白含量达到11.18%,与对照组相比增加了38.54%;纤维素由22.74%降低到22.32%,降低了1.85%;木质素由12.90%降低到10.53%,降低了18.37%。这些结果证明芦笋老茎经微生物发酵后能转化为良好的高蛋白反刍动物饲料。  相似文献   

Molecular characterization of tetracycline- and quinolone-resistant Aeromonas salmonicida isolated in Korea     

Ji Hyung Kim  Sun Young Hwang  Jee Soo Son  Jee Eun Han  Jin Woo Jun  Sang Phil Shin  Casiano Choresca  Jr  Yun Jaie Choi  Yong Ho Park  Se Chang Park 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(1):41-48

The antibiotic resistance of 16 Aeromonas (A.) salmonicida strains isolated from diseased fish and environmental samples in Korea from 2006 to 2009 were investigated in this study. Tetracycline or quinolone resistance was observed in eight and 16 of the isolates, respectively, based on the measured minimal inhibitory concentrations. Among the tetracycline-resistant strains, seven of the isolates harbored tetA gene and one isolate harbored tetE gene. Additionally, quinolone-resistance determining regions (QRDRs) consisting of the gyrA and parC genes were amplified and sequenced. Among the quinolone-resistant A. salmonicida strains, 15 harbored point mutations in the gyrA codon 83 which were responsible for the corresponding amino acid substitutions of Ser⁸³→Arg⁸³ or Ser⁸³→Asn⁸³. We detected no point mutations in other QRDRs, such as gyrA codons 87 and 92, and parC codons 80 and 84. Genetic similarity was assessed via pulsed-field gel electrophoresis, and the results indicated high clonality among the Korean antibiotic-resistant strains of A. salmonicida.  相似文献   

Mucosa-associated lymphoid tissue lymphoma of the third eyelid conjunctiva in a dog     

Hong IH  Bae SH  Lee SG  Park JK  Ji AR  Ki MR  Han SY  Lee EM  Kim AY  You SY  Kim TH  Jeong KS 《Veterinary ophthalmology》2011,14(1):61-65

A 4-year-old, neutered female Cocker Spaniel was presented to the veterinary clinic for protrusion of the left third eyelid. When the third eyelids from both eyes were everted, lobulated masses were present on the bulbar surface. The left third eyelid had a larger protrusion. There was no apparent associated ocular or systemic involvement. The tumor of left third eyelid was removed and referred for histological examination. Histologically, there were proliferations of lymphoid follicles surrounded by lymphoid cells forming a marginal zone. Those lymphoid cells occasionally infiltrated into conjunctival epithelium. A few apoptotic bodies with karyopyknotic and karyorrhexic nuclei were observed in the germinal center of lymphoid follicles. Mitotic figures were rare. On immunohistochemistry, tumor cells expressed CD79a but not CD3. A diagnosis of extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT) of the third eyelid was established based on the histological and immunophenotypical features. At the 1-year follow-up, there was no evidence of recurrence of the mass at the area of excision of the left third eyelid and the remaining tumor of the right third eyelid was still a similar size. The dog still showed no significant findings, except those of the tumor, and no evidence of systemic involvement. To the authors' knowledge, this is the first reported case of MALT lymphoma of the third eyelid in a dog.  相似文献   

猪伪狂犬病毒和猪细小病毒双重PCR方法的建立及初步应用     

夏应菊  汪葆玥  訾占超  韩雪  曲萍  倪建强  顾小雪  遇秀玲  翟新验  田克恭 《中国动物检疫》2011,28(12):32-35

目的本研究旨在建立一种适用于临床样品和动物源性生物制品中猪伪狂犬病毒和猪细小病毒同时检测的双重PCR技术。方法针对猪伪狂犬病毒(PRV)的gE基因和猪细小病毒(PPV)的VP2基因的保守区域分别设计引物。结果经条件优化后,所建立的双重PCR方法能特异性地检测出样品中的PRV(581bp)和PPV(202bp)。结论本方法具有良好的特异性、敏感性和稳定性,适用于临床样品中对PRV和PPV的同时检测,也可用于猪源性生物制品的检测。  相似文献