Excretion of aflatoxine in milk in experimental aflatoxicosis of dairy cows]     

A Piska?  O Zapletal  P Jagos  R Dvorák  J Bouda 《Veterinární medicína》1978,23(4):219-224

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Hematological symptoms of stress in piglets at weaning     

M Dvorák 《Veterinární medicína》1972,17(2):81-90

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Surveillance of influenza in pig herds in Czechoslovakia in 1974-1979. 2. Antibodies against influenza A (H3N2) and A (H1N1) viruses     

B T?mová  A Stumpa  J Mensík 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1980,27(8):601-607

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The relationship between fermentation and the morphological structure of rumen epithelium in sheep fed pelleted feeds]     

M Baran  J Marcaník  J Kocisová  I Zelenák  M Belák 《Veterinární medicína》1979,24(10):587-595

Wethers were fed complete granular feed rations including 41.81% of grass hay, 25.28% of barley, 15.37% of sawdust, 14.98% of molasses, 1.32% of urea, and 1.24% of mineral supplement in dry matter for 24 weeks. Samples of the dorsal rumen sac of these wethers were subjected to patho-anatomical, histological, histo-chemical, and electron-microscopical examination. Volatile fatty acids were also determined in the rumen fluid of slaughtered animals. The control group was given the same diet in the classical form with long hay. The rumen contents of the slaughtered animals of the experimental group had an increased level of total volatile fatty acids (125.93 mM) and butyric acid (17.8 M%). The acetate:propionate ratio was 3.66. No substantial differences were observed in enzymatic activity. Electronograms recorded an increase in the number of T cells and keratinizing cells -- this suggests an increased intensity of the process of keratinization.  相似文献   

Equine viral arteritis in a newborn foal: parallel detection of the virus by immunohistochemistry, polymerase chain reaction and virus isolation     

Szeredi L  Hornyák A  Dénes B  Rusvai M 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(6):270-274

A 4-days-old foal died after a short course of respiratory syndrome and fever. Large areas of the alveoli, bronchioles and bronchi were partly or completely filled by hyaline membranes. Pronounced oedema and mild interstitial pneumonia were present and, in the small muscular arteries, fibrinoid necrosis and vasculitis or perivasculitis could be seen. Vasculitis was found in several other organs, and it was most severe in the thymus. The virus was detected in the lung, kidney and spleen using virus isolation and in the lung and spleen using polymerase chain reaction. The virus was also detected in several organs and cell types using both N protein-specific monoclonal antibody and horseradish peroxidase-labelled equine arteritis virus-specific equine IgG.  相似文献   

Effect of fast protein liquid chromatography fractionated salivary gland extracts from different ixodid tick species on interleukin-8 binding to its cell receptors   总被引：2，自引：0，他引：2  

Kocáková P  Sláviková M  Hajnická V  Slovák M  Gasperík J  Vancová I  Fuchsberger N  Nuttall PA 《Folia parasitologica》2003,50(1):79-84

Interleukin-8 plays a critical role in inflammatory processes. Hence generation of molecules with anti-IL-8 activity is likely to be important for successful feeding and for survival of the ticks. Anti-IL-8 activity was studied in saliva of three ixodid tick species--Dermacentor reticulatus (Fabricius, 1794), Rhipicephalus appendiculatus Neumann, 1901, and Amblyomma variegatum (Fabricius, 1794). The greatest activity was shown in saliva prepared from D. reticulatus. The activity was attributed to tick salivary gland molecules that bind to IL-8, preventing binding of the chemokine to its specific receptor, rather than to occupation of the IL-8 cell receptor by the tick molecules. The distribution of anti-IL-8 activity in fast protein liquid chromatography (FPLC) fractions of salivary gland extracts (SGE) derived from adult female D. reticulatus, R. appendiculatus and A. variegatum was compared directly by both ELISA and receptor-binding inhibition assays. The correspondence in results with fractions of SGE from ELISA is consistent with detection of tick molecules that inhibit IL-8 binding to its receptor. As IL-8 is an important chemoattractant and activator of neutrophils, the presence of an anti-IL-8 activity in tick saliva indicates that neutrophils play an important role in the host response to parasitism by ticks.  相似文献   

Structure-activity relationship study of alkynyl ether insecticide synergists and the development of MB-599 (verbutin)     

Bertók B  Pap L  Arvai G  Bakonyvári I  Kuruczné Ribai Z 《Pest management science》2003,59(4):377-392

Structure-activity relationships of aryl alkynyl synergists of the general formula of Ar-Q-R, where Q represents a bridging structure, were studied using a standardised testing system and Relative Potency values. Ethers, esters, oxime ethers, amides and amines were prepared and evaluated. The length of the R-alkynyl chain, the role of the bridge and the substitution of the aromatic ring were examined systematically. The most potent compounds possessed an aromatic ring connected via a bridge of three atoms to an alkynyl chain, forming together a linear side-chain of six atoms. Several highly potent compounds were synthesised of which one (MB-599; proposed common name verbutin) was selected for development as a selective insecticide synergist in crop protection. Its high potential at practical insecticide:synergist ratios makes possible the reduction of the total amount of insect-control chemicals applied, and its use as an additive to produce new formulations of existing insecticides makes it highly advantageous in resistance management, giving a new tool to sustain the effectiveness of a wide range of insecticides. A product containing a (1+1) mixture of verbutin and beta-cypermethrin was launched in Hungary in 2002.  相似文献   

Spontaneous antibiotic resistance mutation associated pleiotropic changes in Escherichia coli O157:H7     

Tóth I  Csík M  Emódy L 《Acta veterinaria Hungarica》2003,51(1):29-44

Besides the well-known O157:H7 clone causing enterohaemorrhagic colitis and haemolytic uraemic syndrome in Europe, Japan and North America, the number of Escherichia coli isolates with non-motile (NM) phenotype has considerably increased. We supposed that spontaneous antibiotic resistance mutation could cause this phenotypic change. To model our hypothesis we isolated rifampicin--(Rif) and ampicillin--(Amp) resistant mutants from E. coli O157:H7 prototype strains 7785 and EDL933. Among Rifr mutants we could isolate strains with no or reduced motility, while the Ampr mutants became hypermotile. The biochemical profile of the mutants had not changed but phage sensitivity and generation time of the mutants were altered. Among the representative strains we did not find polymorphism with Southern blot analysis and no polymorphism was found in the fliC gene of the mutants. The described characteristics have proven to be stable. In a mice virulence assay by intravenous infections the virulence of the derivatives was also found to be changed. In summary, we found that the antibiotic-resistant phenotype in E. coli O157:H7 was coexpressed with several other phenotypic changes including motility and virulence. It can be assumed that expression of the involved phenotypes may be under the influence of a common regulatory cascade. Further work is needed to identify the components and mechanism of this regulatory system.  相似文献   

A survey for BVDV antibodies in cattle farms in Slovakia and genetic typing of BVDV isolates from imported animals     

Vilcek S  Mojzisová J  Bajová V  Paulík S  Strojný L  Durkovic B  Hipíková V 《Acta veterinaria Hungarica》2003,51(2):229-236

A serological survey for bovine viral diarrhoea virus (BVDV) antibodies on a collection of 1295 serum samples obtained from 6-12 months old cattle originating from 45 farms in Slovakia was carried out. On 13 farms more than 90% of the examined animals were seropositive, on 14 farms 71-90% seroprevalence was observed, on 13 farms only 50-70% animals were found to be positive for BVDV antibodies, while the remaining 5 farms showed fewer than 50% seropositive animals. The average incidence of BVDV antibodies (around 70%) was similar as determined 30 years ago. Of 84 serum samples from seronegative animals originating from 14 farms in which 70-98% seropositivity was observed, six were positive in Ag-BVDV ELISA indicating persistently infected (PI) cattle. On a farm to which animals were imported from abroad, a BVD outbreak was observed. Of 110 animals tested, four were positive in Ag-ELISA indicating the presence of PI cattle on this farm. Genetic typing of two isolates from imported animals performed by RT-PCR (324/326 primers from 5'-UTR), sequencing of PCR products and computer-assisted phylogenetic analysis revealed that they belong to BVDV-1 h group.  相似文献   

First cases of canine babesiosis in the Slovak Republic     

Chandoga P  Goldová M  Baranová D  Kozák M 《The Veterinary record》2002,150(3):82-84

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