Identification of Differentially Expressed Genes and Lipid Metabolism Signaling Pathways between Muscle and Fat Tissues in Broiler Chickens     

Min Zhang  Dan Zheng  Zhimei Peng  Yuting Zhu  Rongrong Li  Qiong Wu  Yan Li  Hongyi Li  Weihua Xu  Mao Zhang  Qichun Hung  Yanfa Sun 《The Journal of Poultry Science》2021,58(2):131

In this study, signaling pathways and key differentially expressed genes (DEGs) involved in lipid metabolism in muscle and fat tissues were investigated. Muscle and abdominal fat tissues were obtained from 35-day-old female broilers for RNA sequencing. DEGs between muscle and fat tissues were identified. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of DEGs were performed. A total of 6130 DEGs were identified to be significantly enriched in 365 GO terms, most of which were involved in biological processes, cellular components, and molecular functions in muscle and fat tissues. Three important lipid signaling pathways (pyruvate metabolism, the insulin signaling pathway, and the adipocytokine signaling pathway) were identified among the fat and muscle tissues of broilers. The key common DEGs in these pathways included phosphoenolpyruvate carboxykinase 2 (PCK2), acetyl-CoA carboxylase 1 alpha and beta (ACACA and ACACB), and the mitogen-activated protein kinase (AMPK) gene family. Hence, our findings revealed the pathways and key genes and gene families involved in the regulation of fat deposition in the muscle and fat tissues of broilers.  相似文献   

副猪嗜血杆菌单克隆抗体的制备及其B细胞抗原表位的鉴定     

杨玉菊  郑楠  符芳  柴政  姜福成  王香玲  张雪云  王卓  李曦 《中国预防兽医学报》2014,(5):400-403

为筛选具有临床诊断价值的副猪嗜血杆菌(H.parasuis)单克隆抗体(MAb),本研究采用H.parasuis 5型强毒株(HPS-5)免疫BALB/c小鼠,利用常规细胞融合技术制备了一株稳定分泌MAb的杂交瘤细胞株。Western blot结果表明该MAb能够特异性识别所有H.parasuis标准菌株;免疫共沉淀技术与蛋白质谱分析显示该MAb所识别的蛋白为ATP结合盒转运蛋白(ABCT)家族的寡肽透过酶(OppA);利用MAb对噬菌体12肽库进行淘选,8个阳性噬菌体克隆展示有"KTPAE-R"保守序列,对应于H.parasuis SH29755株和SH0165株的469位~475位的氨基酸残基。对OppA氨基酸序列比对结果表明H.parasuis与其它10种猪常见细菌病病原序列一致性为9.1%~74.5%,本研究结果为进一步研究H.parasuis感染的病原学诊断建立了良好的基础。  相似文献   

Genome‐Wide Identification of MicroRNAs Responsive to High Temperature in Rice (Oryza sativa) by High‐Throughput Deep Sequencing          下载免费PDF全文

J. Li  L.‐Q. Wu  W.‐Y. Zheng  R.‐F. Wang  L.‐X. Yang 《Journal of Agronomy and Crop Science》2015,201(5):379-388

MicroRNAs (miRNAs) are known to play important roles in plant growth and stress response. Heat stress is a severe abiotic stresses by adversely affecting plant growth and yield. To identify heat‐responsive miRNAs at the genome‐wide level in rice (Oryza sativa), we constructed two small RNA libraries from young panicles treated or not with heat conditions. Ion torrent sequencing of the two libraries identified 294 known miRNAs and 539 novel miRNAs. Differential expression analysis showed that 26 miRNAs were downregulated and 21 miRNAs were upregulated in response to heat stress. Among them, five heat‐responsive miRNAs, including miR162b, miR529a‐p5, PC‐5P‐62245‐9, miR171b and miR169n, were validated by quantitative real‐time polymerase chain reaction. A total of 44 target genes of the differentially expressed miRNAs were predicted. These target genes are most significantly overrepresented in the cell growth process. The results demonstrated that rice miRNAs play critical roles in the heat stress response. This study opens up a new avenue for understanding the regulatory mechanisms of miRNAs involvement in the heat stress response in rice.  相似文献   

新疆恒源煤矿新近系水体采动等级评价     

秦俊林  胡友彪  许春耕  陈忠胜  郑步连 《绿色科技》2015,(1):216-218

指出了恒源煤矿侏罗系煤系地层基本无水,主要充水水源为新近系独山子组的孔隙-裂隙含水层,但是主井筒开拓时,开始涌水量很小,继续向下开拓时,井筒涌水量超过1000m3/h。计算了煤层"两带"高度和新近系含水层与下覆煤系地层间距,研究表明:煤系地层与新近系水体间并无直接的水力联系,具体的涌水来源尚无定论,估算矿井的最大涌水量为160.74m3/h,正常涌水量为53.58m3/h。  相似文献   

基于无线传感网络隧道亮度信号采集系统的研究     

廖江  郑伟  李良荣 《山东饲料》2015,(5):147-148

亮度信号是高速公路隧道节能照明控制系统必须检测的物理量。本文基于zigbee的技术特点,设计了一款基于无线传感网络的隧道亮度信号采集系统。系统的传感器节点负责亮度信号的采集,经CC2530处理发送,通过逐级跳转的方式把数据传输到协调器。协调器通过RS232串口与灯具控制器通信,以此实现对亮度信号的实时采集和可靠性传输。通过实验表明,该系统达到了设计的目的,并适用于高速公路隧道节能照明控制系统。  相似文献   

基于无线传感网络隧道亮度信号采集系统的研究     

廖江  郑伟  李良荣 《山东饲料》2015,(4)

亮度信号是高速公路隧道节能照明控制系统必须检测的物理量。本文基于zigbee的技术特点，设计了一款基于无线传感网络的隧道亮度信号采集系统。系统的传感器节点负责亮度信号的采集，经CC2530处理发送，通过逐级跳转的方式把数据传输到协调器。协调器通过RS232串口与灯具控制器通信，以此实现对亮度信号的实时采集和可靠性传输。通过实验表明，该系统达到了设计的目的，并适用于高速公路隧道节能照明控制系统。  相似文献   

44个紫花苜蓿品种的酸铝适应性与耐受性评价     

田政  杨正禹  陆忠杰  罗奔  张茂  董瑞 《草业学报》2023,32(3):142-151

为了解紫花苜蓿在贵州地区的适应性及耐酸铝胁迫机理，以44份紫花苜蓿品种为研究对象，研究紫花苜蓿处于酸铝胁迫下的生理变化，并揭示其生理变化与耐酸铝胁迫间的关系。利用基因与环境互作模型对两个地点1年的紫花苜蓿进行产量分析，筛选出阿尔冈金、新疆大叶苜蓿、Trifecta、Vernal和中牧1号苜蓿5个耐酸铝强适应品种。利用敏感型UC-1465和耐受型阿尔冈金进行酸铝胁迫试验。结果表明：相同处理下，耐受型紫花苜蓿的电导率、相对铝含量、死亡率显著低于敏感型；紫花苜蓿对酸铝胁迫的响应主要通过柠檬酸、苹果酸、乙酸、酒石酸、反丁烯二酸和草酸的显著（P<0.05）增加来体现，其中苹果酸的合成和分泌增多可能是其耐酸铝胁迫的重要原因。  相似文献   

日本国土综合开发及对中国的借鉴意义     

郑文晖 《世界农业》2011,(6)

在概述日本国土综合开发历程的基础上,从取得的成效与存在的问题两个方面,全面评价了日本国土综合开发的实施效果,进而从完善规划编制与实施体系、加强开发方式的针对性与多样性、建立健全跨行政地域的协调机制、重视协调经济社会发展与环境保护的关系等4个方面论述日本国土综合开发对中国的借鉴意义.  相似文献   

传统平房仓技术改造双低储粮试验     

张青峰  蒋志坚  杨建春  郑细祥  李首文 《粮食储藏》2014,(2):24-27

通过在传统平房仓实施保温、密闭和制冷技术改造的同时,对仓内存储的成品粮实施绿色低氧保管。解决了成品粮易变质、难度夏的保管难题,确保储粮安全。  相似文献   

缩短500 kV某变电站生产投运时间     

严太山  陶梦江  郑晓琼  柴宏博  史钟玉  何光亮 《农村电气化》2020,(2):59-62

根据国家电网公司《国家电网公司变电运维管理办法》等6项通用制度的规定。通用制度中明确对变电站内设备验收、运维、检修、检测、评价和反事故措施等方面提出了新标准和要求,国家电网要求生产投运周期控制在90天以内。为此,小组对近几年安徽检修分公司所辖500 kV变电站的新建、扩建工程所须时间进行统计分析,结果表明,新站投运所需时间大约在110天左右,离国家电网公司对新建变电站投运时间上的最新要求存在一定差距。另外,生产投运周期过长也严重影响了新站投运、扩建工程的进度,阻碍了电网快速发展,也不符合十三五电网规划的要求。考虑如何提高变电站工程生产投运效率,已成为满足电网规划发展、保障安全供电要求的当务之急。因此,QC小组确定此次QC活动的课题为“缩短500 kV某变电站生产投运时间”。  相似文献