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Alexandre Olvera Maria Ballester Miquel Nofrar��as Marina Sibila Virginia Aragon 《Veterinary research》2009,40(3)
Haemophilus parasuis is a colonizer of the upper respiratory tract of healthy pigs, but virulent strains can cause a systemic infection characterized by fibrinous polyserositis, commonly known as Glässer’s disease. The variability in virulence that is observed among H. parasuis strains is not completely understood, since the virulence mechanisms of H. parasuis are largely unknown. In the course of infection, H. parasuis has to survive the host pulmonary defences, which include alveolar macrophages, to produce disease. Using strains from different clinical backgrounds, we were able to detect clear differences in susceptibility to phagocytosis. Strains isolated from the nose of healthy animals were efficiently phagocytosed by porcine alveolar macrophages (PAM), while strains isolated from systemic lesions were resistant to this interaction. Phagocytosis of susceptible strains proceeded through mechanisms independent of a specific receptor, which involved actin filaments and microtubules. In all the systemic strains tested in this study, we observed a distinct capsule after interaction with PAM, indicating a role of this surface structure in phagocytosis resistance. However, additional mechanisms of resistance to phagocytosis should be explored, since we detected different effects of microtubule inhibition among systemic strains. 相似文献
74.
Gustavo A. Ramírez Jaume Altimira Beatriz García Marcos Fernández† Miquel Vilafranca 《Veterinary dermatology》2009,20(1):35-41
Straelensiosis is a relatively new disease described in dogs and produced by a trombiculid larva known as Straelensia cynotis . Few cases have been reported in the English literature. Straelensiosis has been observed in Southern France and Northern Portugal causing a distinctive nodular dermatitis. The present study describes the clinical, histopathological and epidemiological features of 19 cases diagnosed as straelensiosis in the north of Spain and Portugal (2003–2007). A follow-up of the animals after diagnosis was carried out. Differences with previously described cases were observed, especially concerning epidemiology and histopathology. The grade of response to different treatments and new microscopic features are discussed. The study concludes that S. cynotis can cause a nodular dermatitis in rural and hunting dogs in Spain and Portugal and is associated with particular seasonal and geographical factors. 相似文献
75.
Casas M Pujols J Rosell R de Deus N Peralta B Pina S Casal J Martín M 《Veterinary microbiology》2009,135(3-4):248-252
The objective of the present work was to ascertain the date in which hepatitis E virus (HEV) was introduced in the Spanish pig population. For this, a serological retrospective study was carried out using archived sera. A total of 2871 serum samples gathered between 1985 and 1997 and collected in 208 farms of Spain were tested for anti-HEV IgG by an in-house ELISA. Of the 2871 sera analyzed by ELISA, 1390 were positive for anti-HEV antibodies (48.4%, 95% CI: 46.9-49.9%) and that corresponded to 204/208 farms (98%, 95% CI: 96.1-99.9%) having at least one positive pig. Our results show that HEV was present and widespread in Spanish swine farms at least since 1985. Any significant changes in prevalence were detected from 1 year to another and therefore, HEV infection in swine should be considered endemic in Spain. 相似文献
76.
A multiplex RT-PCR assay for the rapid and differential diagnosis of classical swine fever and other pestivirus infections 总被引:2,自引:0,他引:2
Heidy Díaz de Arce Lester J. Prez Maria T. Frías Rosa Rosell Joan Tarradas Jos I. Núez Llilianne Ganges 《Veterinary microbiology》2009,139(3-4):245-252
Classical swine fever is a highly contagious viral disease causing severe economic losses in pig production almost worldwide. All pestivirus species can infect pigs, therefore accurate and rapid pestivirus detection and differentiation is of great importance to assure control measures in swine farming. Here we describe the development and evaluation of a novel multiplex, highly sensitive and specific RT-PCR for the simultaneous detection and rapid differentiation between CSFV and other pestivirus infections in swine. The universal and differential detection was based on primers designed to amplify a fragment of the 5′ non-coding genome region for the detection of pestiviruses and a fragment of the NS5B gene for the detection of classical swine fever virus. The assay proved to be specific when different pestivirus strains from swine and ruminants were evaluated. The analytical sensitivity was estimated to be as little as 0.89 TCID50. The assay analysis of 30 tissue homogenate samples from naturally infected and non-CSF infected animals and 40 standard serum samples evaluated as part of two European Inter-laboratory Comparison Tests conducted by the European Community Reference Laboratory, Hanover, Germany proved that the multiplex RT-PCR method provides a rapid, highly sensitive, and cost-effective laboratory diagnosis for classical swine fever and other pestivirus infections in swine. 相似文献
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78.
Wireless sensor network deployment for integrating video-surveillance and data-monitoring in precision agriculture over distributed crops 总被引:6,自引:0,他引:6
Antonio-Javier Garcia-Sanchez Felipe Garcia-Sanchez Joan Garcia-Haro 《Computers and Electronics in Agriculture》2011,75(2):288-303
Monitoring different parameters of interest in a crop has been proven as a useful tool to improve agricultural production. Crop monitoring in precision agriculture may be achieved by a multiplicity of technologies; however the use of Wireless Sensor Networks (WSNs) results in low-cost and low-power consumption deployments, therefore becoming a dominant option. It is also well-known that crops are also negatively affected by intruders (human or animals) and by insufficient control of the production process. Video-surveillance is a solution to detect and identify intruders as well as to better take care of the production process. In this paper, a new platform called Integrated WSN Solution for Precision Agriculture is proposed. The only cost-effective technology employed is IEEE 802.15.4, and it efficiently integrates crop data acquisition, data transmission to the end-user and video-surveillance tasks. This platform has been evaluated for the particular scenario of scattered crops video-surveillance by using computer simulation and analysis. The telecommunications metrics of choice are energy consumed, probability of frame collision and end-to-end latency, which have been carefully studied to offer the most appropriate wireless network operation. Wireless node prototypes providing agriculture data monitoring, motion detection, camera sensor and long distance data transmission (in the order of several kilometers) are developed. The performance evaluation of this real tests-bed scenario demonstrates the feasibility of the platform designed and confirms the simulation and analytical results. 相似文献
79.
80.
A 6‐year‐old entire male black Labrador retriever was presented with nonpruritic multicentric, well‐demarcated alopecia of 12‐weeks duration. Skin biopsies from the margins of alopecic regions showed sebaceous adenitis and sub‐follicular panniculitis. Biopsies from alopecic areas showed severe follicular atrophy with residual fibrous tracts, loss of sebaceous glands and lymphohistiocytic panniculitis beneath individual atrophic hair follicle groups. These features differed from previous reports of pilosebaceous diseases of dogs and appeared to extend the spectrum of inflammatory patterns in presumed immune‐mediated adnexal diseases of this species. During the 12‐month follow‐up, there was partial hair regrowth without treatment but alopecia was permanent in the centre of larger lesions. 相似文献