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81.
82.

Background

Phenotyping of genotype-by-environment interactions in the root-zone is of major importance for crop improvement as the spatial distribution of a plant’s root system is crucial for a plant to access water and nutrient resources of the soil. However, so far it is unclear to what extent genetic variations in root system responses to spatially varying soil resources can be utilized for breeding applications. Among others, one limiting factor is the absence of phenotyping platforms allowing the analysis of such interactions.

Results

We developed a system that is able to (a) monitor root and shoot growth synchronously, (b) investigate their dynamic responses and (c) analyse the effect of heterogeneous N distribution to parts of the root system in a split-nutrient setup with a throughput (200 individual maize plants at once) sufficient for mapping of quantitative trait loci or for screens of multiple environmental factors. In a test trial, 24 maize genotypes were grown under split nitrogen conditions and the response of shoot and root growth was investigated. An almost double elongation rate of crown and lateral roots was observed under high N for all genotypes. The intensity of genotype-specific responses varied strongly. For example, elongation of crown roots differed almost two times between the fastest and slowest growing genotype. A stronger selective root placement in the high-N compartment was related to an increased shoot development indicating that early vigour might be related to a more intense foraging behaviour.

Conclusion

To our knowledge, RADIX is the only system currently existing which allows studying the differential response of crown roots to split-nutrient application to quantify foraging behaviour in genome mapping or selection experiments. In doing so, changes in root and shoot development and the connection to plant performance can be investigated.
  相似文献   
83.
BACKGROUND: Sepsis is still a major cause of death in both human and veterinary medicine. Early diagnosis is essential for appropriate treatment. Identification of patients at risk for developing sepsis is already possible in human medicine through the measurement of plasma interleukin-6 (IL-6) levels. In veterinary medicine, however, this has been investigated only in canine experimental models. OBJECTIVES: The purpose of this study was to measure IL-6 plasma levels in dogs with naturally occurring systemic inflammatory response syndrome (SIRS) and sepsis and to analyze the value of IL-6 as a predictive parameter for severity and mortality. METHODS: Included in the study were 79 dogs that had been admitted to the small animal clinics of Munich and Berlin from July 2004 to July 2005 and that satisfied the diagnostic criteria for SIRS and sepsis as defined using established parameters. Measurement of plasma IL-6 levels on days 0, 1, and 2 was performed by the use of a colorimetric bioassay based on IL-6-dependent cell growth. RESULTS: Septic foci were identified in 43 patients (septic group), and 36 patients were enrolled in the SIRS group. The frequency of positive blood cultures was 11%. The overall mortality rate was 48%. Higher plasma IL-6 levels on the day of admission were significantly correlated with a more severe degree of disease, increased mortality rate, and earlier fatality. CONCLUSIONS: Plasma IL-6 concentration is predictive of outcome in canine SIRS and sepsis and may be a valuable laboratory parameter for assessing critically ill dogs.  相似文献   
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85.
BACKGROUND: Collection of 50 mL of blood (standard unit) in cats is a common procedure. There are several studies on the health status of donors, but to our knowledge there are no reports on the effects of blood collection on the feline donor. HYPOTHESIS: Collection of a standard unit of blood from cats does not significantly change arterial blood pressure (BP), mean arterial pressure (MAP), systolic arterial pressure (SAP), diastolic arterial pressure (DAP), PCV, and heart rate (HR) in healthy blood donor cats. ANIMALS: Twenty-six healthy blood donor cats (6 spayed females and 20 castrated males). METHODS: An oscillometric method was used to measure MAP, SAP, DAP, and to quantify HR before and after blood collection; PCV was obtained before and immediately after blood collection. RESULTS: Despite a significant decrease (P < .05) in all variables (ie, BP, PCV, HR) after blood collection, no adverse events were observed. CONCLUSIONS AND CLINICAL IMPORTANCE: The collection of a unit of blood for transfusion from healthy donor cats weighing more than 5 kg appears to be safe, but this procedure leads to a decrease in arterial BP, PCV, and HR.  相似文献   
86.
This paper analyses the water management performance of small holder irrigation systems in Zimbabwe. The government and farmer managed systems are compared in terms of their ability to match desired with actual water supply. Desired supply is defined as crop water requirements adjusted downwards by rainfall where relevant. The Theil measure of accuracy of forecasts is used to calculate the error committed by each system in trying to match water supply and demand. The analysis shows that, everything else being equal, the farmer managed system performs better than the government system in matching supply and demand. This means that the farmer managed systems should be encouraged for future small holder irrigation development in Zimbabwe.  相似文献   
87.
 Processes that govern the soil nitrogen (N) supply in irrigated lowland rice systems are poorly understood. The objectives of this paper were to investigate the effects of crop rotation and management on soil N dynamics, microbial biomass C (CBIO) and microbial biomass N (NBIO) in relation to rice N uptake and yield. A maize-rice (M-R) rotation was compared with a rice-rice (R-R) double-cropping system over a 2-year period with four cropping seasons. In the M-R system, maize (Zea mays L.) was grown in aerated soil during the dry season (DS) followed by rice (Oryza sativa L.) grown in flooded soil during the wet season (WS). In the R-R system, rice was grown in flooded soil in both the DS and WS. Three fertilizer N rates (0, 50 or 100 kg urea-N ha–1 in WS) were assigned to subplots within the cropping system main plots. Early versus late crop residue incorporation following DS maize or rice were established as additional treatments in sub-subplots in the second year. In the R-R system, the time of residue incorporation had a large effect on NO3 -N accumulation during the fallow period and also on extractable NH4 +-N, rice N uptake and yield in the subsequent cropping period. In contrast, time of residue incorporation had little influence on extractable N in both the fallow and rice-cropping periods of the M-R system, and no detectable effects on rice N uptake or yield. In both cropping systems, CBIO and NBIO were not sensitive to residue incorporation despite differences of 2- to 3-fold increase in the amount of incorporated residue C and N, and were relatively insensitive to N fertilizer application. Extractable organic N was consistently greater after mid-tillering in M-R compared to the R-R system across N rate and residue incorporation treatments, and much of this organic N was α-amino N. We conclude that N mineralization-immobilization dynamics in lowland rice systems are sensitive to soil aeration as influenced by residue management in the fallow period and crop rotation, and that these factors have agronomically significant effects on rice N uptake and yield. Microbial biomass measurements, however, were a poor indicator of these dynamics. Received: 31 October 1997  相似文献   
88.
Physical and chemical soil degradation hinder the introduction of mixed cropping systems on former tea lands in the Central Highlands of Sri Lanka. For instance on two typical soil catenas soil properties such as texture, pH, acidity parameters, pedogenic oxides and available plant nutrients indicating fertility are presented. Obviously Al3+ occupies a larger part of the exchange complex and accounts for more soil acidity than H+. Al toxicity is hence a major restricting factor for plant growth in the area. The investigated data also permit an evaluation of soil erodibility and a soil fertility assessment according to the Fertility Capability Classification System (Sanchez et al., 1982). Agricultural measures to reduce soil acidity and cover the lime requirements of the soils are advised. Additional soil conservation measures should diminish soil erosion in order to improve the sustainability of the cropping systems.  相似文献   
89.
Soil organic matter dynamics after the conversion of arable land to pasture   总被引:4,自引:0,他引:4  
 Conversion of arable land (maize) to pasture will affect the soil organic matter (SOM) content. Changes in the SOM content were studied using a size- and density-fractionation method and 13C analysis. Twenty-six years of maize cropping had resulted in a depletion of carbon stored in the macro-organic fractions (>150 μm) and an increase in the <20 μm fraction. Maize-derived carbon in the upper 20 cm increased from 10% in the finest fraction (<20 μm) to 91% in the coarse (>250 μm), light (b.d. <1.13 g cm–3) fractions. Pasture installation resulted in a rapid recovery of the total SOM content. Up to 90% of the pasture-derived carbon that was mineralized during maize cropping was replaced within 9 years. Especially the medium and coarse size (>150 μm) and light (b.d. <1.13 g cm–3) fractions were almost completely regenerated by input of root-derived SOM. The amount of medium-weight and heavy macro-organic fractions (>150 μm; b.d. >1.13 g cm–3) in the 0- to 20-cm layer was still 40–50% lower than in the continuous pasture plots. Average half-life times calculated from 13C analyses ranged from 7 years in the light fractions to 56 years in heavy fractions. Fractionation results and 13C data indicated that mechanical disturbance (plowing) during maize cropping had resulted in vertical displacement of dispersed soil carbon from the 0- to 20-cm layer down to 60–80 cm. Conversion of arable land to pasture, therefore, not only causes a regeneration of the soil carbon content, it also reduces the risk of contaminant transport by dispersed soil carbon. Received: 10 March 1998  相似文献   
90.
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