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Ex situ collections offer the potential to reduce extinction risks, affording option to society in maintaining future breeding opportunities for productivity and heritage traits. However, how much should we be seeking to collect and conserve in gene banks, and where? We developed a mathematical model to optimize logistical decisions of breed conservation choices and to evaluate alternative scenarios for efficiently re‐allocating genetic materials currently stored in different European gene banks, allowing for cross‐country collections, cost and cryogenic capacity differentials. We show how alternative allocations for the breeds that are currently stored in 11 European gene banks could reduce overall conservation costs by around 20% by selecting cryogenic banks that have relatively lower combination of fixed and collection costs, and are geographically closer to collection regions. Our results show that centralizing collections in one gene bank would double the costs, relative to collective European collections approaches. We also calculate marginal costs of collections and show that increasing diversity within the gene banks implies in higher costs per conserved breed.  相似文献   
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The objective of this study was to provide Flemish greenhouse farmers an accurate growth model for butterhead lettuce, based on two environmental parameters, i.e. soil temperature and short-wave radiation. During two consecutive years, a total of 27 growth experiments were followed up, whereby head fresh weight (at a 14 d interval), soil temperature at 10 cm depth (on a half-hour basis), and short-wave radiation (14 d summation) were measured. Separate Gompertz functions, with either radiation or soil temperature as input variable, accurately modelled growth; but via a combined approach, an almost perfect fit (R2 = 0.91) between measured and simulated head fresh weight was obtained. This modelling approach provides lettuce growers a tool for the quantitative estimation of crop weight in relation to changes in soil temperature and short-wave radiation. Weather forecast (radiation) and managerial decisions (soil temperature), now serve as the input data of a scientifically based lettuce growth model.  相似文献   
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The environmental yardstick for pesticides has been developed as a tool for farmers to select pesticides with the least environmental impact and to quantify the impact of their use at the crop/farm level, regional level and national level. For each pesticide the yardstick assigns environmental impact points for the risk to water organisms, the risk of groundwater contamination and the risk to soil organisms. It is presently used in the Netherlands as a management tool for farmers and technical consultants, as a tool for monitoring the environmental performance of farmers, as a tool for setting standards for ecolabels and as a policy evaluation tool.  相似文献   
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Quinolone resistance is studied and reported increasingly in isolates from humans, food-producing animals and companion animals. Resistance can be caused by chromosomal mutations in topoisomerase genes, plasmid-mediated resistance genes, and active transport through efflux pumps. Cross sectional data on quinolone resistance mechanisms in non-pathogenic bacteria from healthy veal calves is limited. The purpose of this study was to determine the prevalence and characteristics of quinolone resistance mechanisms in Escherichia coli isolates from veal calves, after more than 20 years of quinolone usage in veal calves. MIC values were determined for all isolates collected as part of a national surveillance program on antimicrobial resistance in commensal bacteria in food-producing animals in The Netherlands. From the strains collected from veal calves in 2007 (n=175) all isolates with ciprofloxacin MIC ≥ 0.125 mg/L (n=25) were selected for this study, and screened for the presence of known quinolone resistance determinants. In this selection only chromosomal mutations in the topoisomerase type II and IV genes were detected. The number of mutations found per isolate correlated with an increasing ciprofloxacin MIC. No plasmid-mediated quinolone resistance genes were found. The contribution of efflux pumps varied from no contribution to a 16-fold increase in susceptibility. No correlation was found with the presence of resistance genes of other antimicrobial classes, even though all quinolone non-wild type isolates were resistant to 3 or more classes of antibiotics other than quinolones. Over twenty years of quinolone usage in veal calves in The Netherlands did not result in a widespread occurrence of plasmid-mediated quinolone resistance, limiting the transmission of quinolone resistance to clonal distribution.  相似文献   
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Pesticide resistance management needs an indication of the risk of resistance developing in pests against pesticide applications. This paper describes an evaluation system for the ranking of these risks. The term pests includes all organisms which are causing economic damage in agriculture, including weeds and plant pathogens. The system distinguishes six broad risk categories. It is based on expert judgement of answers to a maximum of ten questions on crop husbandry, pest biology and pest control. The system has been developed for registration purposes in The Netherlands, and is currently being discussed within the European and Mediterranean Plant Protection Organisation (EPPO). ©1997 SCI  相似文献   
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