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51.
Forest fragmentation affects seed production, but little is known about how seeds are distributed in fragmented forests after primary dispersion and how this affects their interactions with other organisms. This study addressed these issues in a fragmented oak forest of Quercus laeta where four habitat types were recognized: interior and edge of patches, deforested matrix around patches, and an abandoned field. Areas and distances to the abandoned field were measured for 40 patches, and acorns were sampled in all habitats. Only samples from the interior and edge of patches had acorns, which were classified as aborted, viable, and parasitized by insects or fungi. Acorns in all these classes were regressed against patch area and distance to the abandoned field. Field experiments were also conducted to determine whether acorn removal rates varied across habitats. Aborted acorns within patches were not related to area or distance, but they decreased with increasing values of both variables at patch edges. Within patches, viable acorns were positively related to increases in area and distance, while the converse occurred for insect-damaged acorns; fungal-infested acorns were not related to patch variables in this habitat. Complex interactions between area and distance explained how acorns in these classes were distributed across patch edges. Acorn removal was higher within patches and their edges when compared to human-disturbed habitats; both mice and birds were identified as potential acorn dispersers. These results suggest that forest fragmentation affects acorn-mediated processes and that this may influence the recovery of patch connectivity in the landscape.  相似文献   
52.
The Chilean blue mussel Mytilus chilensis is an important commercial species. However, little has been published on the population genetics of this species, despite the need to implement management and conservation policies. Randomly amplified polymorphic DNA‐polymerase chain reaction analysis was used to estimate genetic variation within and between eight natural populations along the whole range of its Chilean natural distribution (ca. 1900 km from Arauco (VIII Region) to Punta Arenas (XII Region)). The values of Nei's unbiased genetic distance, D (0.030–0.107), among populations were small, despite the large geographic separation. A mantel test using 50 000 randomizations showed evidence for a significant correlation (r=0.74, P<0.05) between genetic and geographic (coastal) distance. Punta Arenas population was the most genetically differentiated from the others, although the scale of differentiation was not large (D=0.076–0.107). The levels of gene flow (Nm=1.55) found in this study prevent differentiation among populations by genetic drift. This is the result of the long‐lived planktotrophic larvae of M. chilensis, which provides this species with considerable dispersal ability throughout its range, which is favoured by the ocean currents along the Chilean coast. A restricted larval dispersal towards the north due to the Cape Horn Current derived from the West Wind Drift could be the cause of the higher genetic differentiation of Punta Arenas population from the northern populations. For management purposes of the M. chilensis fishery, the results provide no evidence for discrete stocks, with the possible exception of the Punta Arenas population. The present study provides the baseline data in order to continue further characterization of these mussel populations, considering the great increase in aquaculture of this species.  相似文献   
53.
Spermatophore deterioration in pond-reared Penaeus vannamei was studied, and spermatophore quality was evaluated and improved. Sperm production of males collected from grow-out ponds was increased by two techniques: eyestalk ablation using a fresh frozen maturation diet (13% body weight); and laboratory culture using a diet of the formulated feed Nicovita Plus (3% body weight/ d) and frozen squid (2%). Findings are complementary to previous reports that eyestalk ablation improves quality of spermatophores in young (25.7 g) males. The timing of eyestalk ablation for activation of the endocrine mechanism, leading to improved spermatophore quality was also explored. After eyestalk ablation, performed between postmolt and intermolt stages, 26 g males required a minimum of three spermatophore regenerations or 42 d to significantly increase spermatophore size and sperm count. On the other hand, the laboratory culture (2.5 mo) technique improved the quality of spermatophores in successive regenerations for non-ablated males. In the present study, subadult P. vannamei produced spermatophores which, if not transferred or manually ejaculated, gradually deteriorated (successive stages are described), while a new compound spermatophore was being synthesized.  相似文献   
54.
Transmission electron microscopy was applied to sperm removed from males and females belonging to Litopenaeus vannamei, L. stylirostris and L. occidentalis. It was discovered that a region named filamentous meshwork (FM), located between the nucleus and the hemispherical cap, develops differently in these three closely related species. In L. vannamei, the FM is synthesized in the male reproductive system, but seems to complete its formation after mating. In L. stylirostris, the FM region was not present in spermatophores collected from males or in sperm from the thelycum. In L. occidentalis, the FM region is fully developed in male sperm. It is suggested that completion of the FM is required for acrosome maturation, and the process continues after mating in some species of Litopenaeus. In vitro induction of the acrosome reaction in sperm from males and females of L. occidentalis demonstrated for the first time that reactivity is significantly superior in sperm cells that have been attached to the open thelycum for some hours, as compared to sperm in males (prior to transfer). This finding suggests that matured sperm cells of L. occidentalis become capacitated to react against egg water after mating.  相似文献   
55.
White Spot Syndrome Virus (WSSV) has decimated the shrimp aquaculture around the world. Breeding efforts to generate resistant stocks are necessary but there is a lack of basic information on challenge test strategies focused on genetic selection. Infection routes and developmental stages were evaluated on Penaeus vannamei as a first step in a strategy to select white spot virus (WSSV)-resistant stocks. Mortalities could not be induced before the PL30 stage. The impact of infection by immersion and blended tissue was intermediate on mortalities when compared to the minced tissue treatment on PL30. Blended and minced tissue treatments produced the highest mortalities on PL40 while immersion was intermediate. A general tendency towards higher susceptibility associated with older stages was detected. Additionally, juveniles of 1 g average weight from three local breeding programs were challenged. There were no differences in survival between the programs, although two of them derived their progenies from survivors of strong WSSV events. The implications of these results to the WSSV epidemiological characteristics and breeding programs are discussed.  相似文献   
56.
  1. The Pyrenean desman (Galemys pyrenaicus) is an endangered stream-dwelling insectivore endemic to the Iberian Peninsula and the north of the Pyrenees. It favours riffles over slow habitats such as runs or pools, yet it is still unclear whether habitat preference is based on prey availability or on other factors, such as mechanical constraints on its hunting habits.
  2. Desman diet and prey selection were analysed along the pristine Elama Stream and the slightly modified Leitzaran Stream in the Basque Country, Spain.
  3. In each stream the prey consumed were identified by metabarcoding 94 desman faeces, and prey availability characterized from 10 Surber samples taken in each habitat type (riffles, runs and pools). Invertebrates were sorted and identified, the biomass of each taxon was calculated, and their DNA was also extracted, amplified using PCR, and sequenced to build a reference database.
  4. Prey availability and diet varied between streams. Desmans positively selected shredders and invertebrates that live on the substrate, and selected against taxa that live in fine sediment, taxa with hard shells or those that are highly mobile. The diet of desmans was more selective in the Elama, the stream with better ecological status. Food availability did not differ among habitats, and therefore does not explain the preference of desmans for riffles; rather, mechanical constraints to deal with buoyancy and physical habitat heterogeneity were hypothesized to be the reason behind the habitat preference.
  5. The results suggest that promoting the formation of riffles in streams can improve the situation of some desman populations living in degraded habitats.
  相似文献   
57.
The effect of hydroxypropyl-β-cyclodextrin (HPβCD) in the dyeing of polyamide fabric was investigated under conditions that mimic industrial dyebaths where dye mixtures are used. The dyes used were Telon Red BN, Telon Yellow A-2R, and Telon Blue RR. The blue dye complexed with HPβCD whereas the other dyes did not do so under the conditions used. The dyeing results were compared with those obtained using a traditional retarding agent, Albegal B. The exhaustion dyeing data showed that HPβCD mainly affected the dyeing behavior of the blue dye that became encapsulated. In the case of the yellow and red dyes, changes in the overall kinetics of dyeing were observed, resulting in modified exhaustion profiles. In comparison, Albegal B retarded the blue dyeing process and increased the rate of exhaustion of the yellow dye while the exhaustion curve of red dye remained unchanged. The color uniformity of the dyed polyamide was improved when HPβCD was used. This resulted from the more controlled exhaustion rate during the critical phase of the dyeing process.  相似文献   
58.
Plant Foods for Human Nutrition - There is great interest in the search for new alternatives to antimicrobial drugs, and the use of prebiotics and probiotics is a promising approach to this...  相似文献   
59.
Irrigation with treated wastewater (TWW) is gaining importance due to declining water availability in dry regions. TWW irrigation has various potential adverse effects on soil quality such as hydrophobic effects on soil surfaces, reducing initial sorptivity and promoting the formation of preferential flow paths. In May and June 2010, in situ infiltration measurements using mini disk tension infiltrometer were deployed in five different orchard plots in Israel to assess the impact of different irrigation water qualities on the soil water repellency index R. In most plantations, long-term test sites were accessed to compare adjacent plots irrigated with fresh water (FW) or TWW. Topsoil samples were analyzed for selected physical and chemical characteristics. The mean R values increased at all TWW sites, from +15 up to +55 % compared with FW sites. The water drop penetration time (WDPT) increased up to 30 fold at three of five TWW sites compared with FW sites. Subsequent U tests and multilevel analysis indicated an impact of the type of irrigation water on R and WDPT. Moreover, soil electrical conductivity and exchangeable sodium percentage were consistently higher at all TWW sites. These results show that irrigation water quality clearly influences physical and chemical properties of the soil.  相似文献   
60.
Drip irrigation systems are prone to changes in flow rate (FR) and increasing coefficients of variation (CV) when fed with treated wastewater, due to fouling inside the drippers. A model system was designed to measure the FR and fouling accumulation in laterals and drippers under different treatment conditions. A novel approach was taken to compare the different maintenance treatments. A comparison of chlorination and acidification strategies showed that daily chlorination and periodic acidification may prolong proper functioning of the drippers by maintaining a normal FR [(up to ±7 %) of nominal FR] and CV (<7 %) index in correlation with low fouling accumulation in the pipeline (<0.01 mg deposit/cm pipe 2 ). Current recommendations for the frequency of conventional treatments were found to be insufficient. Chemical analyses of the fouling inside the dripper and accumulated on the pipe wall showed that biofilm can survive inside the dripper under harsh environmental conditions, even when the pipeline stays clean. These results shed light on biofilm growth and survival mechanisms inside the dripper and may pave the way to developing new treatments or improving dripper design.  相似文献   
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