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991.
Tropical Animal Health and Production - The aim of the present study was to develop and evaluate an equation to predict body weight (BW) using hip width (HW) in Pelibuey ewe lambs and ewes. Five...  相似文献   
992.
The objective of the study was to evaluate the effects of litter size and supplementation of functional amino acids on the piglet birth weight variability through of a systematic review and meta-analysis. PubMed, ISI Web of Science, Science Direct, Scopus and SciELO are the main used databases and were searched in January 2019 by using the following keywords and their combinations: within-litter birth, weight variability, piglets, litter size, sow, amino acids, gestation, uniformity, CV and/or SD. Of the 64 pre-selected articles, only articles presenting the coefficients of variation and/or standard deviations according to the litter size and different levels of amino acid supplementation in the sow diets during gestation were selected. The average birth weights of total and live piglets were 43% lower in litters from sows with high prolificacy than those from sows with low prolificacy. The weight variability was affected by high prolificacy sows, with increases of 4.04% and 4.54% in the coefficients of variation of total and born alive piglets respectively (p = .074; p = .009). The standard deviation increased by 180 g in born alive piglets to high prolificacy sows. The coefficients of variation of total and live piglets born from high prolificacy sows showed an increase of 4.04% and 4.54% respectively (p = .07; p < .05). There was a tendency for reduced weight variability with amino acids supplementation when considering the standard deviation of total born (p = .072). However, reproductive performance was not significantly influenced (p > .05). In conclusion, the average litter weight and the number of piglets born influence weight variability. Amino acids supplementation may reduce the birth weight variability between piglets. However, this effect depends on the amino acid used, the levels of supplementation and on other factors not completely addressed in this study. Thus, more research is necessary to fully elucidate this topic.  相似文献   
993.
994.
Erwinia psidii is a gram-negative bacterium that threatens both guava and eucalypt plantations in several countries. Despite the economic importance of both crops, nothing is currently known about the molecular mechanisms underlying E. psidii pathogenicity and, consequently, how it evolved to infect Eucalyptus species besides its presumed native host Psidium guajava. In this study, we predicted putative type III secretion system effectors that may play important roles during plant–E. psidii interactions and conducted effector structure and phylogenetic analyses to gain important insights into their function and evolution. For that, the whole genomes of four E. psidii strains that exhibit differential aggressiveness towards eucalypt clones were sequenced and their effector repertoires predicted based on sequence identity with known effectors of the model phytopathogen Erwinia amylovora. Only proteins sharing significant sequence identity with the DspE and Eop1 effectors were found. Here, it is shown that these two E. psidii effectors retain all structural characteristics of their corresponding protein superfamilies, but exhibit allelic variations that are consistent with the observed aggressiveness differences between strains. Phylogenetic analyses revealed that whereas E. psidii housekeeping gene sequences are more closely related to those from Erwinia tracheiphila, the effector (either nucleotide or amino acid) sequences are more closely related to their Pantoea agglomerans counterparts, suggesting that dspE and eop1 were both acquired through horizontal gene transfer from the latter bacterial species. The results of this study provide important insights on E. psidii pathogenicity and set the stage for future effector functional studies.  相似文献   
995.
Phytoparasitica - The Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae) is a key vector of the causal agents of huanglongbing (HLB) in important citrus production regions of the...  相似文献   
996.
Journal of Plant Diseases and Protection - Limited information is available on the distribution of grapevine red blotch virus (GRBV) in grape-growing regions outside North America. In this study,...  相似文献   
997.
Red stripe is a bacterial disease of sugarcane causing important economic losses in Argentina that affects 30 % of the milling stems and consequently the juice quality. In this study, sugarcane leaves exhibiting red stripe symptoms were sampled in the 2008–09 growing season from 13 different sugarcane producing areas of Tucumán and Salta (northwest of Argentina). To achieve the identification and characterization of the causal agent of red stripe, bacterial isolation was performed. Species-specific PCR using Oaf1/Oar1 primers allowed the amplification of a fragment of 550 bp from approximately 50 % of the isolates; 16S rDNA sequences analysis displayed a similarity greater than 99 % with Acidovorax avenae subsp. avenae. By means of RAPD-PCR the presence of at least four different biotypes among the analyzed isolates was detected. Results of pathogenicity test allowed us to confirm A. avenae subsp. avenae as the pathogenic agent for red stripe. This study constitutes the first report on the identification and molecular characterization of this plant pathogen from the Argentina sugarcane production areas. The genetic diversity observed among A. avenae is an important factor to be considered to improve an accurate diagnosis and/or the selection of sugarcane tolerant clones.  相似文献   
998.
The antioxidant responses of Solanum nigrum L. cell suspension cultures to metalaxyl exposure were investigated. An increase in lipid peroxidation and hydrogen peroxide content, for both concentrations tested (20 mg L1; 40 mg L1) revealed the response of oxidative metabolism of cell suspensions to metalaxyl. Superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6) and ascorbate peroxidase (APX; EC 1.11.1.11) activities increased, particularly in the highest concentration of metalaxyl used. An analysis by non-denaturing polyacrylamide gel (PAGE) followed by staining for enzyme activity, revealed seven SOD isoenzymes, two CAT isoenzymes, and nine APX isoenzymes. Metalaxyl levels were quantified in the culture medium and results suggest that suspension cells were able to accumulate and/or degrade the fungicide five hours after exposure. SOD, CAT and APX isoenzymes were differently affected by the metalaxyl treatment. Results suggest that the higher concentration of metalaxyl induced oxidative stress to cell suspension cultures of S. nigrum.  相似文献   
999.
Although tetracyclines and macrolides are common additives for animal nutrition, methods for their simultaneous determination in animal feeds are nonexistent. By coupling an organic extraction and solid-phase extraction cleanup to a high-performance liquid chromatography separation and a nonaqueous postcolumn derivatization, we succeeded in detecting from 0.2 to 24.0 μg kg(-1) of tetracycline, oxytetracycline, chlortetracycline, doxycycline, tigecycline, and 4-epitetracycline in this complex and heterogeneous matrix. Minocycline and tylosin could also be detected with our procedure, but using UV spectrophotometry (1.5 ≤ LOD ≤ 1.9 mg kg(-1)). Linear responses with correlation coefficients between 0.996 and 0.999 were obtained for all analytes in the 0.5-10 mg kg(-1) concentration range. Average recoveries between 59 and 97% and between 98 and 102% were obtained for the tetracyclines and tylosin, respectively. Replicate standard deviations were typically below 5%. When this method was applied to 20 feeds marketed in Costa Rica, we detected labeling inconsistencies, banned mixtures of tetracyclines, and tetracycline concentrations that contravene international regulation.  相似文献   
1000.
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