Identification of mutation in the growth differentiation factor 5 (Gdf5) gene in NC-brp/brp mice     

Suto J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(10):1121-1124

A brachypodism (brp) mutation arose spontaneously in the inbred NC mouse strain, producing a phenotype similar to that caused by bp mutation; therefore, it is strongly suggested that brp and bp are allelic. A series of bp mutations are due to defects in the growth differentiation factor 5 (Gdf5) gene. Nucleotide sequence analysis on the Gdf5 gene in NC-brp/brp mice revealed that an irregular insertion of a unit ;GGCAGCC' in exon 2 caused a frame shift leading to a premature stop codon. In addition to the known physiologic roles of brp, I found that brp significantly reduced the litter size. The brp is a novel mutant allele at the Gdf5 gene locus; I would like to name this allele Gdf5(brp).  相似文献   

Quantitative trait loci that control plasma lipid levels in an F2 intercross between C57BL/6J and DDD.Cg-A(y) inbred mouse strains     

Suto J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(4):449-456

The objectives of this study were to characterize plasma lipid phenotypes and dissect the genetic basis of plasma lipid levels in an obese DDD.Cg-A(y) mouse strain. Plasma triglyceride (TG) levels were significantly higher in the DDD.Cg-A(y) strain than in the B6.Cg-A(y) strain. In contrast, plasma total-cholesterol (CHO) levels did not substantially differ between the two strains. As a rule, the A(y) allele significantly increased TG levels, but did not increase CHO levels. Quantitative trait locus (QTL) analyses for plasma TG and CHO levels were performed in two types of F(2) female mice [F(2)A(y) (F(2) mice carrying the A(y) allele) and F(2) non- A(y) mice (F(2) mice without the A(y) allele)] produced by crossing C57BL/6J females and DDD.Cg-A(y) males. Single QTL scan identified one significant QTL for TG levels on chromosome 1, and two significant QTLs for CHO levels on chromosomes 1 and 8. When the marker nearest to the QTL on chromosome 1 was used as covariates, four additional significant QTLs for CHO levels were identified on chromosomes 5, 6, and 17 (two loci). In contrast, consideration of the agouti locus genotype as covariates did not detect additional QTLs. DDD.Cg-A(y) showed a low CHO level, although it had Apoa2(b), which was a CHO-increasing allele at the Apoa2 locus. This may have been partly due to the presence of multiple QTLs, which were associated with decreased CHO levels, on chromosome 8.  相似文献   

Effects of coumestrol administration to maternal mice during pregnancy and lactation on renal Ca metabolism in neonatal mice     

Michihisa UEDA  Yoshihiro HORIGUCHI  Miki SUGIMOTO  Shuntaro IKEDA  Shinichi KUME 《Animal Science Journal》2012,83(6):469-473

The present study was conducted to clarify the effects of coumestrol administration to maternal mice during pregnancy and lactation on serum Ca and Ca metabolism in their neonatal mice. From 6.5 to 16.5 days post coitus and from 3 to 10 days after parturition, maternal mice were administered at 200 µg/kg body weight/day of coumestrol. Coumestrol administration did not affect weight gains, serum Ca and the expression of vitamin D receptor (VDR) protein in the kidney of neonatal mice, but weight gains of maternal mice were decreased by coumestrol administration. Coumestrol administration increased the messenger RNA (mRNA) expressions of epithelial Ca channels 1 (ECaC1) and VDR in the kidney of neonatal mice, and also increased the mRNA expressions of ECaC2 in the kidney and small intestine of male neonatal mice. The mRNA expressions of ECaC1, ECaC2, calbindin‐D_9k (CaBP‐9k) and estrogen receptor (ER)α in the kidney and VDR in the small intestine of male neonatal mice were higher than those of female mice. Thus, coumestrol administration to maternal mice during pregnancy and lactation may affect renal Ca metabolism in neonatal mice, especially male neonatal mice via maternal milk.  相似文献   

Effect of volume of non-surgical embryo transfer medium on ability of porcine embryos to survive to term     

Nakazawa Y  Misawa H  Fujino Y  Tajima S  Misumi K  Ueda J  Nakamura Y  Shibata T  Hirayama Y  Kikuchi K 《The Journal of reproduction and development》2008,54(1):30-34

Non-surgical embryo transfer is a promising method for improving efficiency in the pork industry and also for biotechnology applications, such as in vitro embryo production, transgenesis and cloning. Several groups have reported successful piglet production using an artificial insemination catheter or flexible catheter designed for this procedure; however, the efficiency of the technique is still low. The critical points that need to be addressed in order to improve this procedure are (1) the embryo deposition site and (2) volume of transfer medium associated with the embryos; however, the latter has not yet been examined systematically. In the present study, we evaluated the effect of the volume of non-surgical embryo transfer medium on the ability of porcine embryos to survive to term by using a recently produced flexible catheter. The catheter consists of a guide and an injector. Blastocysts 200-230 mum in diameter were collected from donor gilts and transferred to recipient gilts. The time required for the completion of embryo transfer using this catheter was 14.6 +/- 3.9 min. The tip of the injector was determined by laparotomy to be located in a uterine horn 20-30 cm anterior from the branching point of the uterus body. We transferred 17.0-17.3 embryos with different volumes of medium (1.6, 3.2 and 10 ml) into each of 5, 4 and 4 recipients, respectively, and pregnancy was confirmed in 4, 3 and 1 of these recipients, respectively. Three recipients in the 1.6 ml group farrowed a total of 19 piglets (4, 5 and 10 piglets, respectively). These results suggest that successful non-surgical embryo transfer is affected by the volume of transfer medium.  相似文献   

拖拉机沿曲线路径的跟踪控制   总被引：6，自引：2，他引：6  

陈军  朱忠祥  鸟巢谅  武田纯一 《农业工程学报》2006,22(11):108-111

近年关于农业用车轮型移动机器人的研究很多,但主要集中在直线行走方面。该研究进行了沿给定的曲线路径跟踪控制的研究。首先由给定的曲线路径生成四元状态空间,其次在利用预见控制求得车辆的未来值和目标值的基础上,利用最优控制理论设计跟踪控制器。最后在牧草地上对正弦路径和圆形路径进行了跟踪实验。试验结果为：车辆以1.5 m/s的速度行驶时,在很小转弯半径时最大误差可控制在35 cm以内。  相似文献   

Identification and characterization of proteoglycans in bovine neonatal skeletal muscle     

Takanori NISHIMURA  Eiko FUTAMI  Jun-ichi WAKAMATSU  Akihito HATTORI 《Animal Science Journal》2003,74(5):407-416

In order to provide background for understanding biological roles of proteoglycans (PG) in developing skeletal muscle, we have isolated and characterized PG in bovine neonatal skeletal muscle. Two types of PG were isolated from skeletal muscle by density gradient ultracentrifugation and ion‐exchange chromatography. One was a small PG (PG‐S) with a molecular size of 100–130 kDa, another was a large PG (PG‐L) with a molecular size of 300–500 kDa. The glycosaminoglycan chains of PG‐S and PG‐L were dermatan sulfate and chondroitin sulfate, respectively, judged by cellulose acetate membrane electrophoresis. Immunoblot assays revealed that both PG bound to type I, II, III and IV collagen, laminin and fibronectin. Unlike PG‐S, PG‐L bound to type V collagen and hyaluronic acid. Small proteoglycans had a core protein of 45 kDa, which reacted with the antibody against the decorin core protein. The N‐terminal amino acid sequence of the PG‐S core protein was consistent with that of decorin from bovine bone and tendon. Thus, PG‐S from neonatal skeletal muscle was identified as decorin in bovines. Immunohistochemical analysis with antibodies against PG‐L and PG‐S demonstrated that PG‐L was located both in the perimysium and endomysium, but PG‐S was localized exclusively in the perimysium. These findings suggest that the characterized PG may have distinct roles in the ECM construction of developing skeletal muscle.  相似文献   

Further mapping and characterization of Naq1, a quantitative trait locus responsible for maternal inferior nurturing ability in RR mice     

Suto J  Sekikawa K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(9):1033-1038

Females of the inbred mouse RR strain have a limited ability to nurture their offspring, and frequently the young die during rearing. We previously identified a significant quantitative trait locus (QTL) responsible for the inferior nurturing ability on chromosome 5 (Naq1), on the basis of litter weight of six pups at days 7, 12, and 21 after parturition. Here, we carried out further mapping of Naq1 to define the confidence interval precisely. At the same time, we analyzed new quantitative trait variables, litter weight gain between days 7 and 12 (WG1), and that between days 12 and 21 (WG2), to characterize further the physiology of inferior nurturing ability. Consequently, a peak LOD score for the Naq1 was identified on D5Mit218 (72 cM), which was located approximately 2 cM distal to our previous expectation, as a significant QTL for WG1 (LOD 5.5), but not for WG2 (LOD 0.9). Because the growth of pups depends purely on milk obtained from the dam up to day 12 after birth, it seems possible to assume that the inferior nurturing ability in RR mice is related to defects in maternal nutritional support (that is, lactation) rather than to defects in pup growth. Naq1 is a novel QTL as far as the QTL results of relevant female reproductive traits in cattle and pigs are concerned.  相似文献   

The Effects of Calcitonin on the Development of and Ca2+ Levels in Heat-shocked Bovine Preimplantation Embryos In Vitro     

Shumpei KAMANO  Shuntaro IKEDA  Miki SUGIMOTO  Shinichi KUME 《The Journal of reproduction and development》2014,60(4):317-323

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Behavior of nitrogen in the media supplied with the aqueous phase produced by methane fermentation as liquid fertilizer     

Hisatoshi MIYATA  Hideo IKEDA 《Soil Science and Plant Nutrition》2006,52(1):135-135

pp. 859–864
Behavior of nitrogen in the media was investigated when the aqueous phase produced by methane fermentation was supplied as liquid fertilizer.
When the aqueous phase was supplied to masa-soil,      included in masa-soil was nitrified to      . Additionally, in masa-soil,       absorption was observed. On the other hand, in the case of coconut fiber and rockwool,     has been hardly nitrified. From above results, it was determined that       toxic effect from the aqueous phase on tomato plants growth was inhibited by means of the progress of nitrification and       absorption by masa-soil.
  However, nitrification was restrained so that       was fixed in masa-soil. On the other hand, in the sampled field soil,       was not fixed, and was nitrified immediately. In the field soil, there was a great deal of generated      . In comparison with masa-soil.  相似文献   

Factors affecting expression and transcription of uncoupling protein 2 gene     

Doo Hyun KIM  Hiroyuki SADAKANE  Yuka NISHIKIORI  Manami MATSUMURA  Mayuko IKEDA  Zhicheng DIAO  Rajesh JHA  Masaru MURAKAMI  Tohru MATSUI  Masayuki FUNABA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(12):1734

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