Effect of antineoplastic drugs on the expression of Bcl-2 and Bcl-xL genes in the feline T-cell leukemia cell line     

Sano J  Nagafuchi S  Yamazaki J  Oguma K  Kano R  Hasegawa A 《Research in veterinary science》2005,79(3):197-201

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Peculiar Composition of Epithelial Cells in Follicle-Associated Intestinal Crypts of Peyer’s Patches in the Rat Small Intestine     

Youhei MANTANI  Hideto YUASA  Miho NISHIDA  Ei-ichirou TAKAHARA  Takuya OMOTEHARA  Kankanam Gamage Sanath UDAYANGA  Junichi KAWANO  Toshifumi YOKOYAMA  Nobuhiko HOSHI  Hiroshi KITAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(6):833-838

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Construction of a high-density reference linkage map of tea (Camellia sinensis)     

Fumiya Taniguchi  Kazumi Furukawa  Sakura Ota-Metoku  Nobuo Yamaguchi  Tomomi Ujihara  Izumi Kono  Hiroyuki Fukuoka  Junichi Tanaka 《Breeding Science》2012,62(3):263-273

A few linkage maps of tea have been constructed using pseudo-testcross theory based on dominant marker systems. However, dominant markers are not suitable as landmark markers across a wide range of materials. Therefore, we developed co-dominant SSR markers from genomic DNA and ESTs and constructed a reference map using these co-dominant markers as landmarks. A population of 54 F₁ clones derived from reciprocal crosses between ‘Sayamakaori’ and ‘Kana-Ck17’ was used for the linkage analysis. Maps of both parents were constructed from the F₁ population that was taken for BC₁ population. The order of most of the dominant markers in the parental maps was consistent. We constructed a core map by merging the linkage data for markers that detected polymorphisms in both parents. The core map contains 15 linkage groups, which corresponds to the basic chromosome number of tea. The total length of the core map is 1218 cM. Here, we present the reference map as a central core map sandwiched between the parental maps for each linkage group; the combined maps contain 441 SSRs, 7 CAPS, 2 STS and 674 RAPDs. This newly constructed linkage map can be used as a basic reference linkage map of tea.  相似文献   

Evaluation of iron bioavailability from bonito dark muscle using anemic rats     

Matsumoto J  Mori N  Doi M  Kishida T  Ebihara K 《Journal of agricultural and food chemistry》2003,51(15):4478-4482

The bioavailability of iron from ferrous sulfate (FeII-S), heme iron prepared from hemoglobin (HIP), and bonito dark muscle (BDM) was assessed in anemic rats using a hemoglobin regeneration efficiency (HRE) method. Freeze-dried BDM (FD), boiled and freeze-dried BDM (B/FD), and boiled and smoke-dried BDM (B/SD) were used as BDM source. Rats were made anemic by feeding on an iron-deficient diet for 28 days. To replete their iron levels, anemic rats were then fed on a diet containing iron at a level of 17 ppm for 14 days. Rats receiving FeII-S gained significantly more weight and had greater food intake and higher HRE compared to the other four groups. The bioavailability of iron from HIP was poor compared with that from FeII-S and BDM. When the HRE of rats fed FeII-S was 100, that of rats fed BDF was approximately 80. These results suggest that BMD is an effective dietary source of iron.  相似文献   

Characteristics of wastewater treatment using a multi-soil-layering system in relation to wastewater contamination levels and hydraulic loading rates   总被引：2，自引：0，他引：2  

Tsugiyuki MASUNAGA  Kuniaki SATO  Junichi MORI  Matsusige SHIRAHAMA  Hiromoto KUDO  Toshiyuki WAKATSUKI 《Soil Science and Plant Nutrition》2007,53(2):215-223

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Requirement of phospholipase Cdelta4 for the zona pellucida-induced acrosome reaction     

Fukami K  Nakao K  Inoue T  Kataoka Y  Kurokawa M  Fissore RA  Nakamura K  Katsuki M  Mikoshiba K  Yoshida N  Takenawa T 《Science (New York, N.Y.)》2001,292(5518):920-923

Several phospholipase C (PLC) isoforms have been found in male and female mammalian gametes, and splicing isoforms of PLCdelta4 are predominantly expressed in testis. Here we report that male mice in which the PLCdelta4 gene had been disrupted either produced few small litters or were sterile. In vitro fertilization studies showed that insemination with PLCdelta4-/- sperm resulted in significantly fewer eggs becoming activated and that the calcium transients associated with fertilization were absent or delayed. PLCdelta4-/- sperm were unable to initiate the acrosome reaction, an exocytotic event required for fertilization and induced by interaction with the egg coat, the zona pellucida. These data demonstrate that PLCdelta4 functions in the acrosome reaction that is induced by the zona pellucida during mammalian fertilization.  相似文献   

干,湿谷混合贮藏干燥的试验研究     

上出顺一  李长友 《华南农业大学学报》1993,14(2):121-126

本文介绍在日本国寺泉干燥中心测得的干湿谷混合干燥试验结果,分析混合干燥设施的能量消耗、干燥效率、物料品质等项技术指标。获得了混合干燥新技术开发的基础性数据。  相似文献   

Immunohistochemical and histoplanimetrical study on the spatial relationship between the settlement of indigenous bacteria and the secretion of bactericidal peptides in rat alimentary tract     

Yokoo Y  Miyata H  Udayanga KG  Qi WM  Takahara E  Mantani Y  Yokoyama T  Kawano J  Hoshi N  Kitagawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):1043-1050

To clarify the regulatory mechanism by bactericidal peptides secretion, the secretion of bactericidal peptides was immunohistochemically and histoplanimetrically compared with the degree of Gram-positive/negative bacterial colonization throughout the rat alimentary tract. In the associated exocrine glands from the oral cavity to the stomach, no comparable differences were observed under the changes of development of indigenous bacterial colonies. In the small intestine, immunopositive granules for lysozyme and secretory phospholipase A2 (sPLA2) were markedly decreased, whereas immunopositive vacuoles in the Paneth cells were more increased at sites with hyper-development of indigenous bacterial colonies in the intervillous spaces than at sites with no or less development. No changes in exocrine glands were observed in the large intestine because of the constant existence of large quantities of bacteria. Gram-positive bacterial colonies on the mucosal surfaces were dominant from the oral cavity to the stomach. Gram-negative bacteria were dominant in the large intestine, and the distributions of both Gram-positive and negative bacteria were intermediate in the small intestine. These findings suggest that lysozyme and sPLA2 secreted from the Paneth cells contribute to the regulation of the proliferation of indigenous bacteria in the intervillous spaces of the small intestine, and that the inversion of distributions of Gram-positive and -negative bacteria in the alimentary tract might be caused by the secretion of lysozyme and sPLA2 in the small intestine.  相似文献   

Background Data for General Toxicology Parameters in RccHan:WIST Rats at 8, 10, 19 and 32 Weeks of Age     

Okamura T  Suzuki S  Ogawa T  Kobayashi J  Kusuoka O  Hatayama K  Mochizuki M  Hoshiya T  Okazaki S  Tamura K 《Journal of toxicologic pathology》2011,24(4):195-205

Recently, RccHan(TM):WIST (Wistar Hannover) rats were introduced to toxicity studies in Japan. The present study was performed to obtain control data for general toxicological parameters as an aid for interpretation of results in toxicity studies using this strain of rats. Four test groups comprising of 25 male and 25 female RccHan(TM):WIST rats were housed for 2, 4, 13 or 26 weeks from 6 weeks of age and observed and examined for clinical observation, body weight, food consumption, urinalysis, hematology, blood chemistry, organ weight, necropsy and/or histopathology. Ophthalmological examination was not conducted in this study, and the data in this report were obtained from an ongoing 104-week background study in RccHan(TM):WIST rats. These data were compared with the historical control data of CD(SD) (Sprague-Dawley) and/or F344 (Fischer) rats. The body weights of RccHan(TM):WIST rats were lower than those of CD(SD) rats and higher than those of F344 rats. The ophthalmological examination revealed a greater incidence of focal corneal opacity. Histopathology revealed focal mineralization of the cornea and Berlin blue-positive pigmentation in the epididymal interstitium as well as hepatocytes. Other than the above, some minor differences were found in urinalysis, hematology, blood chemistry and organ weights as compared with CD(SD) rats.  相似文献   

Epigenetically immature oocytes lead to loss of imprinting during embryogenesis     

Obata Y  Hiura H  Fukuda A  Komiyama J  Hatada I  Kono T 《The Journal of reproduction and development》2011,57(3):327-334

Loss of imprinting (LOI) is occasionally observed in human imprinting disorders. However, the process behind the LOI is not fully understood. To gain a better understanding, we produced embryos and pups from mouse oocytes that lacked a complete methylation imprint using a method that involved transferring the nuclei of growing oocytes into the cytoplasm of enucleated fully grown oocytes following in vitro fertilization (IVF). We then analyzed the imprinting statuses. Our findings show that the incomplete methylation imprint derived from growing oocytes results in epigenetic mosaicism or a loss of methylation imprint (LOM) at maternal alleles in embryos. In some embryos, both hypo- and hypermethylated maternal Kcnq1ot1 alleles were detected, whereas either hypo- or hypermethylated maternal Kcnq1ot1 alleles were detected in others. Such tendencies were also observed at the Igf2r and Mest loci. Gene expression levels of imprinted genes were linked with their methylation statuses in some but not all embryos. Possible explanations of the inconsistency between the data from DNA methylation and gene expression include epigenetic mosaicism in embryos. Pups were successfully produced from growing oocytes at a quite low frequency. They exhibited an obese phenotype and LOI with respect to Igf2r, Snrpn and Mest. Our finding suggests the possibility that LOI/LOM at maternal alleles in human concepti could be derived from epigenetically immature/mutated oocytes.  相似文献