Modelling exploration of the future of European beech (Fagussylvatica L.) under climate change—Range,abundance, genetic diversity and adaptive response     

Koen Kramer  Bernd Degen  Jutta Buschbom  Thomas Hickler  Wilfried Thuiller  Martin T. Sykes  Wim de Winter 《Forest Ecology and Management》2010

We explored impacts of climate change on the geographic distribution of European beech by applying state of the art statistical and process-based models, and assessed possible climate change impacts on both adaptive capacity in the centre of its distribution and adaptive responses of functional traits at the leading and trailing edge of the current distribution. The species area models agree that beech has the potential to expand its northern edge and loose habitat at the southern edge of its distribution in a future climate. The change in local population size in the centre of the distribution of beech has a small effect on the genetic diversity of beech, which is projected to maintain its current population size or to increase in population size. Thus, an adaptive response of functional traits of small populations at the leading and trailing edges of the distribution is possible based on genetic diversity available in the local population, even within a period of 2–3 generations.  相似文献   

Protein display by bovine herpesvirus type 1 glycoprotein B     

Günther M. Keil  Constanze KlopfleischKatrin Giesow  Jutta Veits 《Veterinary microbiology》2010

Glycoprotein B (gB) of bovine herpesvirus 1 (BHV-1), a major component of the viral envelope, is essential for membrane fusion during entry and cell-to-cell spread. It is cleaved in the trans-Golgi network by the proprotein convertase furin. Integration of the open reading frame (ORF) encoding a mutated gB with a second furin cleavage site and mature boIFN-α as intervening peptide between the amino-terminal (NH₂) and carboxy-terminal (COOH) gB subunits yielded recombinant BHV-1/gB2FuIFN-α which, unexpectedly, express gB with an enlarged NH₂-subunit of 90 kDa. Here we show that boIFN-α-specific antibodies bind to the 90 kDa gB subunit and efficiently neutralize BHV-1/gB2FuIN-α infectivity. We also show that inactivated BHV-1/gB2FuIN-α virions induce an antiviral state in cells incubated with UV-inactivated particles. These results demonstrate that the 90 kDa protein is a NH₂-subunit/boIFN-α fusion protein whose boIFN-α domain is biologically active. To verify that BHV-1 gB is suitable for the display of (glyco)proteins on the surface of virions we constructed BHV-1 recombinants expressing within gB the first 273 amino acids of the NH₂-subunit (HA1) of avian influenza haemagglutinin, either flanked by two furin cleavage sites or with only one cleavage site between a gB/NH₂_HA1 fusion protein and the COOH subunit. The resulting recombinant BHV-1/gB2FuHA1 expressed gB from which 55 kDa HA1 was excised and secreted. In contrast, gB from BHV-1/gB_NH₂HA1 infected cells retained HA1 as fusion protein with the NH₂-subunit. Immunoblotting and neutralization analyses revealed that HA1 is incorporated into the envelope BHV-1/gB/NH₂_HA1 particles and exposed to the exterior of virions. Thus, this novel approach enables display of polypeptides and (glyco)proteins of at least 273 amino acids on viral particles which is of particular interest for development of novel diagnostics and vaccines as well as for, e.g. gene therapy applications especially when biologically active ligands need to be presented.  相似文献   

The invasive ergot Claviceps purpurea var. spartinae recently established in the European Wadden Sea on common cord grass is genetically homogeneous and the sclerotia contain high amounts of ergot alkaloids     

Christian Boestfleisch  Astrid M. Drotleff  Waldemar Ternes  Stefan Nehring  Sylvie Pažoutová  Jutta Papenbrock 《European journal of plant pathology / European Foundation for Plant Pathology》2015,141(3):445-461

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Isolation and molecular characterisation of equine rotaviruses from Germany   总被引：2，自引：0，他引：2  

Elschner M  Schrader C  Hotzel H  Prudlo J  Sachse K  Eichhorn W  Herbst W  Otto P 《Veterinary microbiology》2005,105(2):123-129

A total of 26 rotavirus positive faecal samples of diarrhoeal foals, and 8 equine rotavirus isolates were examined. Viral RNA patterns were generated, G typing was performed by PCR, and a P[12]-specific DNA probe was developed for P typing. Furthermore, five equine rotavirus isolates were sequenced in the genomic regions coding for VP7 and part of VP4. Rotaviruses of genotype G3 P[12] were found in 22 faecal samples and G14 P[12] type could be found in 4 faecal samples. These findings confirm that in Germany G3 P[12] is the predominating type of equine rotaviruses.  相似文献   

Primary immune-mediated thrombocytopenia in 30 dogs (1997-2003)     

Putsche JC  Kohn B 《Journal of the American Animal Hospital Association》2008,44(5):250-257

During a 6-year period, primary (idiopathic) immune-mediated thrombocytopenia was retrospectively evaluated in 30 dogs. Ages of the dogs ranged from 3 months to 10 years (median 4 years); female dogs were markedly overrepresented with 73%. Clinical examination revealed hemorrhages in 70% of the dogs. Platelet counts ranged from 0 to 111,000/microL (median 8000/microL); 77% of the dogs had platelet counts <30,000/microL. Seventeen dogs were anemic (hematocrit 9% to 36%; median 31%). Immunosuppressive therapy was performed in all but one dog. The recurrence rate of 19 dogs that were followed over an extended period (112 to 1684 days; median 340 days) was 26%. Twenty-nine (97%) dogs survived 14 days after initial presentation, and 27 (93%) dogs survived at least the following 15 to 1684 days (median 220 days).  相似文献   

Determination of Polycyclic Aromatic Hydrocarbons in Wastewater,Sediments, Sludge and Plants in Karak Province,Jordan     

Jiries  Anwar  Hussain  Helmi  Lintelmann  Jutta 《Water, air, and soil pollution》2000,121(1-4):217-228

Analysis for sixteen types of Polycyclic Aromatic hydrocarbons (PAHs)from samples of raw and treated wastewater, sediments, sludge andplants growing along treated wastewater way was performed inOctober 1997. The collection sites represent two wastewatertreatment plants (WWTP) receiving different types of wastewaterand one site from disposal of raw wastewater in the city ofKarak in southern Jordan.Wastewater treatment efficiency showed removal of PAHs throughsettling tanks and adsorption on sediments after treatment. Theremoval percentage ranged from 44–100% for individual PAH.PAHs were widely distributed at various levels in rawwastewater, treated wastewater, sludge, sediments and plants.The highest concentration was observed in sludge and the lowestin plants. There was a variation in PAHs concentration betweenthe three investigated sites which was attributed to theefficiency of treatment, period of contact with the wastewater,and the nature of activities. The WWTPs were capable or reducingPAHs contamination in water, up to 40% of the total PAHs byadsorption on sludge and sediments.  相似文献   

Nature’s Lab for Derivatization: New and Revised Structures of a Variety of Streptophenazines Produced by a Sponge-Derived Streptomyces Strain     

Anna Lena Kunz  Antje Labes  Jutta Wiese  Torsten Bruhn  Gerhard Bringmann  Johannes F. Imhoff 《Marine drugs》2014,12(4):1699-1714

Eight streptophenazines (A–H) have been identified so far as products of Streptomyces strain HB202, which was isolated from the sponge Halichondria panicea from the Baltic Sea. The variation of bioactivities based on small structural changes initiated further studies on new derivatives. Three new streptophenazines (I–K) were identified after fermentation in the present study. In addition, revised molecular structures of streptophenazines C, D, F and H are proposed. Streptophenazines G and K exhibited moderate antibacterial activity against the facultative pathogenic bacterium Staphylococcus epidermidis and against Bacillus subtilis. All tested compounds (streptophenazines G, I–K) also showed moderate activities against PDE 4B.  相似文献   

A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction     

Elisabeth J. Faassen  Maria G. Antoniou  Wendy Beekman-Lukassen  Lucie Blahova  Ekaterina Chernova  Christophoros Christophoridis  Audrey Combes  Christine Edwards  Jutta Fastner  Joop Harmsen  Anastasia Hiskia  Leopold L. Ilag  Triantafyllos Kaloudis  Srdjan Lopicic  Miquel Lürling  Hanna Mazur-Marzec  Jussi Meriluoto  Cristina Porojan  Yehudit Viner-Mozzini  Nadezda Zguna 《Marine drugs》2016,14(3)

Exposure to β-N-methylamino-l-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimer’s disease and Parkinson’s disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D₃BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%–32%), implying that D₃BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery (<10%). Most BMAA was found in a trichloroacetic acid soluble, bound form and we recommend including this fraction during analysis.  相似文献   

Recombinant viruses of poultry as vector vaccines against fowl plague     

Fuchs W  Veits J  Mettenleiter TC 《Berliner und Münchener tier?rztliche Wochenschrift》2006,119(3-4):160-166

To help in the control of fowl plague caused by highly pathogenic avian influenza A viruses of hemagglutinin (HA) subtypes H5 and H7 several vaccines have been developed. A prophylactic immunization of poultry with inactivated influenza viruses in non-endemic situations is questionable, however, due to the impairment of serological identification of field virus-infected animals which hinders elimination of the infectious agent from the population. This problem might be overcome by the use of genetically engineered marker vaccines which contain only the protective influenza virus hemagglutinin. Infected animals could then be unambiguously identified by their serum antibodies against other influenza virus proteins, e.g. neuraminidase or nucleoprotein. For such a use, purified HA or HA-expressing DNA vaccines are conceivable. Economically advantageous and easier to apply are modified live virus vaccines in use against other poultry diseases, which have been modified to express influenza virus HA. So far, recombinant HA-expressing fowlpox virus (FPV) as well as infectious laryngotracheitis and Newcastle disease viruses have been asssessed in animal experiments. An H5-expressing FPV recombinant is already in use in Central America and Southeast Asia but without accompanying marker diagnostics. Advantages and disadvantages of the different viral vectors are discussed.  相似文献   

Einfluß von Bodeneigenschaften auf die Aufnahme von radioaktivem Strontium durch Pflanzen. I. Untersuchungen an Böden Schleswig-Holsteins mit Weißklee und Weidelgras     

Jutta Günther  Diedrich Schroeder 《植物养料与土壤学杂志》1968,119(3):216-226

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