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31.
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Ján BURKU? Martina KA?MAROVá Janka KUBANDOVá Natália KOKO?OVá Kamila FABIANOVá Du?an FABIAN Juraj KOPPEL ?tefan ?IKO? 《The Journal of reproduction and development》2015,61(4):325-331
We found retardation of preimplantation embryo growth after exposure to maternal restraint stress during the preimplantation period in our previous study. In the present study, we evaluated the impact of preimplantation maternal restraint stress on the distribution of inner cell mass (ICM) and trophectoderm (TE) cells in mouse blastocysts, and its possible effect on physiological development of offspring. We exposed spontaneously ovulating female mice to restraint stress for 30 min three times a day during the preimplantation period, and this treatment caused a significant increase in blood serum corticosterone concentration. Microscopic evaluation of embryos showed that restraint stress significantly decreased cell counts per blastocyst. Comparing the effect of restraint stress on the two blastocyst cell lineages, we found that the reduction in TE cells was more substantial than the reduction in ICM cells, which resulted in an increased ICM/TE ratio in blastocysts isolated
from stressed dams compared with controls. Restraint stress reduced the number of implantation sites in uteri, significantly delayed eye opening in delivered mice, and altered their behavior in terms of two parameters (scratching on the base of an open field test apparatus, time spent in central zone) as well. Moreover, prenatally stressed offspring had significantly lower body weights and in 5-week old females delivered from stressed dams, fat deposits were significantly lower. Our results indicate that exposure to stress during very early pregnancy can have a negative impact on embryonic development with consequences reaching into postnatal life. 相似文献
33.
R Malik A Chiu C Klupiec W Lee P Tisdall K DeMaere PJ Canfield 《Australian veterinary journal》2014,92(5):148-148
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Abstract. The sperm of tench, Tinca tinca L., is characterized by a milky colour and consistency, and is of very low density. After collecting the sperm, motion of spermatozoa was recorded even without water activation. A better motility value (value 4·36 on average) was observed in spermatozoa collected in immobilizing solution (collecting medium) and stored for 3h, when compared with spermatozoa without collecting medium. Average total and relative numbers of spermatozoa were 12·16 × 109 per male and 18·50 × 109 per kg of body weight, respectively. When testing the effect of activating solution in artificial propagation of tench, the highest fertilization rates (81·3 and 85% in two cases) were found for NaCl solution with an osmotic concentration of 34 or 69 mOsmol and for fresh water, respectively. The fertility rate was reduced significantly ( P < 0·01) by any increase above 105 mOsmol in NaCl concentration in the activating solution. In the tests of optimal method of artificial fertilization, the highest hatching rate of sac fry (71·35%) was found in sperm collected into immobilizing solution. The application of immobilizing solution significantly increased the number of sac fry at the levels P < 0·1 and P < 0·01, if compared with intact sperm stored for 3 h and fresh sperm, respectively. 相似文献
36.
Tick paralysis in North America and Australia 总被引:1,自引:0,他引:1
The pathophysiology and clinical manifestations of tick paralysis in North America and Australia are reviewed. Clinical and electrodiagnostic findings in tick paralysis are contrasted with those that occur in other diffuse lower motor neuron disorders, and the disease in North America is compared with the more severe form of the disease that occurs along the east coast of Australia. A detailed account of the treatment of animals afflicted with tick paralysis is presented. 相似文献
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M. A. Cheema M. A. Malik A. Hussain S. H. Shah & S. M. A. Basra 《Journal of Agronomy and Crop Science》2001,186(2):103-110
A field study was conducted to investigate the influence of variable rates of application of N and P fertilizers in splits at various times on the growth and the seed and oil yields of canola (Brassica napus L.) during 1995–97. Rates of fertilizer application were 0 and 0 (F0), 60 and 0 (F1), 0 and 30 (F2), 60 and 30 (F3), 90 and 60 (F4) and 120 and 90 (F5) kg N ha?1 and kg P2O5 ha?1. All the P was applied at sowing while N was applied in splits, i.e. all at sowing, half at sowing and half with first irrigation, or half at sowing and half at flowering. The responses of growth, seed yield and components of yield were consistent in both years. Increasing the rate of fertilizer application from F4 (90/60 kg N/P2O5 ha?1) to F5 (120/90 kg N/P2O5 ha?1) increased the leaf area index (LAI) relative to the control and to lower rates of fertilizer application. For both crops, application of 90/60 kg N/P2O5 ha?1 significantly enhanced total dry matter (TDM) and seed yield. Seed yield increased mainly due to a greater number of pods per plant and seeds per seed‐pod. The time of fertilizer application did not significantly affect seed yield or components of yield in either season. Oil yield generally followed seed yield, increasing with increasing rate of fertilizer application up to 90/60 kg N/P2O5 ha?1. The maximum oil contents were obtained from the control. The results show that seed and oil yields of canola were maximized at the F4 (90/60 kg N/P2O5 ha?1) rate of application under the agro‐ecological conditions of Faisalabad, Pakistan. 相似文献
39.
Summary Studies on genetic diversity in chickpea (Cicer arietinum L.) indicated the existence of considerable amount of variation for grain yield and its components in the material. One hundred and thirty two genotypes fell into eight clusters. The covariation structure studied by means of factor analysis indicated the possibility of obtaining, through hybridization, genotypes physiologically and morphologically more efficient. Multivariate analysis of data from 7 parents and 21 F1 hybrids indicated weak correspondence between D2-analysis and canonical variate analysis. As there was no relationship between heterosis over midparent and genetic distance between the parents, so the traditional approach of making a large number of crosses is being suggested. 相似文献
40.
S. K. Malik R. Chaudhury O. P. Dhariwal Rajwant K. Kalia 《Genetic Resources and Crop Evolution》2006,53(7):1485-1493
Citrus indica and C. macroptera are the wild endangered species of Citrus occurring in northeastern India. Surveys were undertaken in this region for ascertaining distribution, studying variability
and for collection of germplasm of these two species. C. indica, an endemic species of this region, was collected from the Citrus Gene Sanctuary located in buffer zone of Nokrek Biosphere
Reserve in the Garo hills of Meghalaya. In addition, a putative natural hybrid of C. indica and C. limon was collected for the first time from the south Garo hills. C. macroptera had much wider distribution and was collected from Mizoram and Meghalaya states. In Jantia hills of Meghalaya, natural populations
of this species are in a highly threatened state. The two species were unevenly distributed all over the explored territory.
Morphological characterization of leaves, fruits and seeds indicated the presence of sizable variability within collected
accessions of these two Citrus species. Indigenous technical knowledge gathered on the use and socio-economic importance indicated commercial potential
for these two species in northeastern India. However, lack of cultivation of these species and clearing of forest cover at
an alarming rate has led to an urgent need to adopt complementary conservation strategies to safeguard these species and to
ensure their availability for future utilization. A major emphasis on developing methods for their propagation, multiplication
and regeneration in in situ and ex situ conditions is required. 相似文献