Protein Content of Shiitake Mushroom Grown on Nitrogen Soaked Sweetgum Logs     

N. A. Sistani  D. T. Ball  C. Sabota 《Journal of plant nutrition》2013,36(8):1279-1286

Shiitake mushrooms are used as a source of protein and meaty flavor in many vegetarian diets. While substrate and geographical location are two factors affecting nutrient content, little research has been done on the nutrient content of shiitake grown in north Alabama. The purpose of this study was to compare the protein and amino acid contents of the ‘West Wind’ strain of shiitake mushrooms grown on sweetgum logs soaked in tap water, versus the protein and amino acid content of the same strain grown on logs soaked in tap water amended with different nitrogen (N) solutions (urea, sulfur-coated urea, and ammonium sulfate), at different rates (0, 3, and 6 g N/L water), and harvested from three successive soakings. Chemical analysis was conducted on samples for protein and amino acids. Analysis of variance showed there was no significant difference in the protein content of shiitake grown on sweetgum logs based on type of nitrogen treatment, rate, or soak period. However, urea showed indications that it may increase protein levels. There was no significant difference in amino acid content based on treatment or rate. Although not statistically significant, there was a trend showing mushrooms from logs soaked at a rate of 3 g N/L water had the highest levels of each amino acid. There were significantly lower levels of amino acids in mushrooms harvested during the summer months. Nitrogen soak solutions did not increase protein or amino acid content, but protein and amino acid contents of shiitake in north Alabama are better understood.  相似文献   

A preliminary survey of Chlamydia psittaci genotypes from native and introduced birds in New Zealand     

KR Gedye  M Fremaux  JC Garcia-Ramirez 《New Zealand veterinary journal》2018,66(3):162-165

AIM: To describe the Chlamydia psittaci genotypes in samples from native and introduced birds from New Zealand by analysis of the sequence variation of the ompA gene.

METHODS: DNA was extracted from samples collected from a non-random sample of birds; either swabs from live asymptomatic birds or birds with clinical signs, or formalin-fixed, paraffin-embedded (FFPE) samples from historical post-mortem cases. The presence of C. psittaci in all samples had been confirmed using a quantitative PCR assay. The C. psittaci ompA gene was amplified and sequenced from samples from 26 native and introduced infected birds comprising 12 different species. These sequences were compared to published available C. psittaci genotypes.

RESULTS: Genotypes A and C of C. psittaci were identified in the samples. Genotype A was identified in samples from nine birds, including various native and introduced species. Genotype C was identified in samples from 16 different waterfowl species, and a mixed infection of both genotypes was found in a kaka (Nestor meridionalis). In native birds, C. psittaci infection was confirmed in seven new host species.

CONCLUSIONS AND CLINICAL RELEVANCE: Two genotypes (A and C) of C. psittaci were found in samples from a wider range of both native and introduced species of birds in New Zealand than previously reported. Both genotypes have been globally associated with significant disease in birds and humans. These initial results suggest the host range of C. psittaci in New Zealand birds is under-reported. However, the prevalence of C. psittaci infection in New Zealand, and the associated impact on avian and public health, remains to be determined. There are biosecurity implications associated with the importation of birds to New Zealand if there is a limited diversity of C. psittaci genotypes present.  相似文献   

Phytoplankton community structure and the drawdown of nutrients and CO2 in the southern ocean     

KR Arrigo  DH Robinson  DL Worthen  RB Dunbar  GR DiTullio  M VanWoert  MP Lizotte 《Science (New York, N.Y.)》1999,283(5400):365-367

Data from recent oceanographic cruises show that phytoplankton community structure in the Ross Sea is related to mixed layer depth. Diatoms dominate in highly stratified waters, whereas Phaeocystis antarctica assemblages dominate where waters are more deeply mixed. The drawdown of both carbon dioxide (CO2) and nitrate per mole of phosphate and the rate of new production by diatoms are much lower than that measured for P. antarctica. Consequently, the capacity of the biological community to draw down atmospheric CO2 and transport it to the deep ocean could diminish dramatically if predicted increases in upper ocean stratification due to climate warming should occur.  相似文献   

Ostertagiasis in calves. I. The effect of control measures on infection levels and body weight gains during the grazing season in Denmark     

SV AA Henriksen  R.J. Jørgensen  P. Nansen  KR Sejrsen  J. Brolund Larsen  S. Klausen 《Veterinary parasitology》1976,2(3):259-272

The investigation was designed to study the gastro-intestinal helminth infection established in yearling calves with reference to fluctuations in the larval contamination of the herbage during the grazing season. In a 2 × 3 factor experiment, comprising six groups, each of six calves, a comparison was made between infection levels, growth rates etc. in calves grazing the same paddock over an entire season and calves moved before the July rise in herbage contamination to paddocks not grazed earlier in the same season. A comparison was also made between animals receiving no anthelminthic treatment, animals treated 3 weeks after the start of the grazing season and again when moved, and animals treated every 3 weeks during the season.Before July there were no weight gain differences between the various groups of calves. During the rest of the season, the calves which were moved gained 647–869 g, while those that remained on the same paddock, and were exposed to a high level of larval contamination gained only 81–361 g per day. The effect of anthelmintic treatment was less pronounced, though significant. The weight gains were correlated to a high degree with the larval contamination levels of the respective paddocks (r = ? 0.79) as well as with the serum pepsinogen (r = ? 0.75) and albumin levels (r = 0.75) of the calves and to a minor degree also with faecal egg counts (r = ? 0.45). The observed weight gain differences could not be accounted for by differences in grass quantity or quality.  相似文献   

Susceptibility to antimicrobials of mastitis‐causing Staphylococcus aureus,Streptococcus uberis and Str. dysgalactiae from New Zealand and the USA as assessed by the disk diffusion test          下载免费PDF全文

KR Petrovski  A Grinberg  NB Williamson  ME Abdalla  N Lopez‐Villalobos  TJ Parkinson  IG Tucker  P Rapnicki 《Australian veterinary journal》2015,93(7):227-233

  相似文献   

Maturity Effects on Mineral Concentration and Uptake in Annual Ryegrass     

G. E. Brink  K. R. Sistani  J. L. Oldham  G. A. Pederson 《Journal of plant nutrition》2013,36(6):1143-1155

ABSTRACT

Annual ryegrass (Lolium multiflorum Lam.) provides livestock feed and captures nutrients from fields receiving manure application. The objective of this study was to determine relationships among maturity and yield, mineral uptake, and mineral concentration. Primary spring growth of ‘Marshall’ ryegrass was harvested every 7 d to 56 d maturity and was fertilized with swine effluent containing 254 and 161 kg nitrogen (N) and 42 and 26 kg phosphorus (P) ha^?1 for two years. Yield increased linearly to a maximum of 13.6 mg ha^?1 after 49 d in 2001 and 8.0 mg ha^?1 after 56 d in 2002. Mineral uptake was highly correlated (r > 0.95) with yield and attained a maximum single harvest of 192 kg N ha^?1 and 32 kg P ha^?1 (mean of two years). Concentration of all minerals except calcium (Ca) declined as ryegrass matured. Low magnesium (Mg) concentration (< 2 g kg^?1 dry matter) increases the risk of hypomagnesemic grass tetany.  相似文献   

Microclimate modifications,growth and yield of intercrops under Hardwickia binata Roxb. based agroforestry system     

Arun K Shanker Scientist  Ram Newaj  P Rai  KR Solanki  K Kareemulla  R Tiwari 《Archives of Agronomy and Soil Science》2013,59(3):281-291

An experiment was conducted by planting Hardwickia binata Roxb. at 200, 400 and 800 trees ha^???1, intercrops viz., Brassica campestris (mustard) and Glycine max (soybean) were sown from the second year onwards in winter and summer respectively at the National Research Centre for Agroforestry, Jhansi, U.P., India with the objectives of evaluating agrosilvicultural potential of the system and to study the effect of tree densities on ecophysiology of understorey crops. Tree growth was affected by both density and intercrop in the initial years of growth. Photosynthetic photon flux density (PPFD) available to the intercrops reduced with increasing densities. Transpiration rate and stomatal conductance in intercrops decreased due to the presence of trees. No significant changes in leaf temperature were observed till the fifth year of the growing season. Yield was significantly higher in pure crop in comparison with all the densities in mustard. Soybean yield under 200 trees ha^???1 was comparable to that of the pure crop. Trees at the density of 200 trees ha^???1 provided a conducive microenvironment to the intercrops.  相似文献   

Macronutrient concentration in plant parts of cotton fertilized with broiler litter in a marginal upland soil     

H. Tewolde  M.W. Shankle  A. Adeli  K.R. Sistani  D.E. Rowe 《Soil & Tillage Research》2009,105(1):1-11

Effectiveness of surface-applied unincorporated broiler litter as a fertilizer relative to conventional inorganic fertilizers under no-till or conventional-till cotton (Gossypium hirsutum L.) production systems in the upland soils of the southern and southeastern USA is not well documented. The objectives of this research were to (1) test if broiler litter improves plant macronutrient (N, P, K, and Mg) nutrition of cotton above that of cotton fertilized with conventional inorganic fertilizers and (2) determine if lack of incorporating litter into the soil reduces macronutrient concentration in cotton plant parts in an upland soil considered marginal for cotton. Six treatments consisting of an unfertilized control, a fertilized standard (STD), two litter-only, and two litter plus inorganic N as urea–ammonium nitrate solution (UAN) were tested in two adjacent fields, one under no-till (NT) and the other under conventional-till (CT) systems. Litter alone, UAN, or a combination of litter plus UAN were applied to supply 101 kg ha⁻¹ plant available N assuming nearly all of the UAN-N and 50% of the total litter N becomes plant available during the cotton growing season. Concentration of N, P, K, and Mg were measured in leaves, stems, and reproductive parts on three or four dates between early flowering and maturity. Cotton fertilized with the litter-only treatments always had less N concentration but greater P and K concentration in leaves, stems, and reproductive parts than cotton that received the STD treatment. Leaf and stem Mg concentration seems to depend on the N concentration in these plant parts. Lack of incorporating litter into the soil reduced N concentration in nearly all plant parts at all growth stages, suggesting some amount of the litter-derived N is lost due to lack of incorporation. Lack of incorporation also reduced leaf and stem Mg concentration, which seemed to be due to its reducing effect on N concentration. Unlike N and Mg, lack of incorporation did not consistently affect concentrations of P and K in all plant parts. Regardless of the incorporation treatment, fertilization with the litter-only treatments increased tissue P and K concentration and supported lint yield exceeding that of the STD without increasing tissue N concentration.  相似文献   

Poultry litter and tillage influences on corn production and soil nutrients in a Kentucky silt loam soil     

K.R. Sistani  F.J. Sikora  M. Rasnake 《Soil & Tillage Research》2008,98(2):130-139

Broiler chicken (Gallus gallus) manure, a rich source of plant nutrients, is generated in large quantities in southeastern USA where many row crops, such as corn (Zea mays L.), are also extensively grown. However, the use of broiler manure as an economical alternative source of nutrients for corn production has not been extensively explored in this region. This study was conducted to examine the use of broiler litter as a source of nutrients for corn production, as influenced by tillage and litter rate, and any residual effects following application. In addition, the consequence of litter application to soil test nutrient levels, particularly P, Zn and Cu, was explored. The treatments consisted of two rates of broiler litter application, 11 and 22 Mg ha⁻¹ on a wet weight basis, and one rate of chemical fertilizer applied under no-till and conventional tillage systems. Treatments were replicated three times in a randomized complete block design. Corn was grown with broiler litter and inorganic fertilizer applied to the same plots each year from 1998 to 2001. In 2002 and 2003, corn was planted no-till, but only N fertilizer was applied in order to make use of other residual litter nutrients. Soil samples were taken yearly in the spring prior to litter application and 4 years after the cessation of litter application to evaluate the status of the residual nutrients in soil. Two years out of the 4-year experiment, broiler litter application produced significantly greater corn grain yield than equivalent chemical fertilizer application and produced similar grain yield in the other 2 years. Corn grain yield was significantly greater under no-till in 1999, but significantly greater under conventional-till in 2000, and no difference between the two tillage systems were observed in 1998 and 2001. With 4 years of litter application, Mehlich-3 P increased from an initial 18 mg kg⁻¹ to 156 mg kg⁻¹ with 11 Mg ha⁻¹ litter and to 257 mg kg⁻¹ with 22 Mg ha⁻¹ litter. For every 6 kg ha⁻¹ of P applied in poultry litter Mehlich-3 P was increased by 1 mg kg⁻¹. Modest increases in Mehlich-3 Cu and Zn did not result in phytotoxic levels. This study indicated that an optimum rate of broiler litter as a primary fertilizer at 11 Mg ha⁻¹ applied in 4 consecutive years on a silt loam soil produced corn grain yields similar to chemical fertilizer under both no-till and conventional tillage systems and kept soil test P, Cu and Zn levels below values considered to be harmful to surface water quality or the crop.  相似文献   

Carbonic acid in the gas phase and its astrophysical relevance     

W Hage  KR Liedl  A Hallbrucker  E Mayer 《Science (New York, N.Y.)》1998,279(5355):1332-1335

In outer space, high-energy irradiation of cryogenic ice mixtures of abundant water and carbon dioxide is expected to form solid carbonic acid. Experiments and thermodynamic analyses show that crystalline carbonic acid sublimates without decomposition. Free-energy considerations based on highly accurate molecular quantum mechanics, in combination with vapor pressures resulting from experimental sublimation rates, suggest that in the gas phase, a monomer and dimer of carbonic acid are in equilibrium, comparable to that of formic acid. Gaseous carbonic acid could be present in comets, on Mars and outer solar system bodies, in interstellar icy grains, and in Earth's upper atmosphere.  相似文献