排序方式： 共有99条查询结果，搜索用时 15 毫秒
91.
Matsuda Y Sameshima T Moriura N Inoue K Nonomura T Kakutani K Nishimura H Kusakari S Takamatsu S Toyoda H 《Phytopathology》2005,95(10):1137-1143
92.
We investigated the effects of rice feeding on growth performance and protein (amino acids) metabolism of weanling piglets. In all, 16 weanling piglets with an average initial weight of 7.5 kg were divided into two groups. One group was fed a corn‐soybean meal‐based diet, and the other was fed a rice‐soybean meal diet, containing around 46% of corn or rice, respectively. A two‐week growth trial was conducted. The average daily gain (p = .025) and feed efficiency (p = .011) in rice‐fed piglets were significantly higher than those in corn‐fed piglets. Liver lysine‐ketoglutarate reductase activity tended to be lower (p = .073) in rice‐fed piglets than in corn‐fed piglets. Plasma urea nitrogen concentration in rice‐fed piglets was significantly lower than that in corn‐fed piglets. Plasma glucose and insulin concentrations were significantly higher in rice‐fed piglets than in corn‐fed piglets. Plasma‐free valine, isoleucine, and tryptophan concentrations were significantly higher in rice‐fed piglets than in corn‐fed piglets. In contrast, plasma histidine concentration was significantly lower in rice‐fed piglets than in corn‐fed piglets. Overall, these results show that rice feeding improves the growth performance and affects the protein (amino acids) metabolism in weanling piglets. 相似文献
93.
Kanako OYAMA Tadashi BABA Shin-ichi KASHIWABARA 《The Journal of reproduction and development》2021,67(1):35
Testis-brain RNA-binding protein (TB-RBP/Translin) is known to contribute to the translational repression of a subset of haploid cell-specific mRNAs, including protamine 2 (Prm2) mRNA. Mutant mice lacking TB-RBP display abnormal spermatogenesis, despite normal male fertility. In this study, we carried out functional analysis of TB-RBP in mammalian cultured cells to understand the mechanism of translational repression by this RNA-binding protein. Although the amino acid sequence contained a eukaryotic translation initiation factor 4E (EIF4E)-recognition motif, TB-RBP failed to interact with EIF4E. In cultured cells, TB-RBP was unable to reduce the activity of luciferase encoded by a reporter mRNA carrying the 3’-untranslated region of Prm2. However, λΝ-BoxB tethering assay revealed that the complex of TB-RBP with its binding partner, Translin-associated factor X (TRAX), exhibits the ability to reduce the luciferase reporter activity by degrading the mRNA. These results suggest that TB-RBP may play a regulatory role in determining the sequence specificity of TRAX-catalyzed mRNA degradation. 相似文献
94.
Yukichi Fujikawa Takahiro Nakanishi Hiroko Kawakami Kanako Yamasaki Masa H Sato Hiroyuki Tsuji Makoto Matsuoka Naohiro Kato 《Rice》2014,7(1):11
Background
The rice interactome, in which a network of protein-protein interactions has been elucidated in rice, is a useful resource to identify functional modules of rice signal transduction pathways. Protein-protein interactions occur in cells in two ways, constitutive and regulative. While a yeast-based high-throughput method has been widely used to identify the constitutive interactions, a method to detect the regulated interactions is rarely developed for a large-scale analysis.Results
A split luciferase complementation assay was applied to detect the regulated interactions in rice. A transformation method of rice protoplasts in a 96-well plate was first established for a large-scale analysis. In addition, an antibody that specifically recognizes a carboxyl-terminal fragment of Renilla luciferase was newly developed. A pair of antibodies that recognize amino- and carboxyl- terminal fragments of Renilla luciferase, respectively, was then used to monitor quality and quantity of interacting recombinant-proteins accumulated in the cells. For a proof-of-concept, the method was applied to detect the gibberellin-dependent interaction between GIBBERELLIN INSENSITIVE DWARF1 and SLENDER RICE 1.Conclusions
A method to detect regulated protein-protein interactions was developed towards establishment of the rice interactome. 相似文献95.
96.
Understanding genetic diversity among local populations is a primary goal of modern crop breeding programs. Here, we demonstrated the genetic relationships of rice varieties in Hokkaido, Japan, one of the northern limits of rice cultivation around the world. Furthermore, artificial selection during rice breeding programs has been characterized using genome sequences. We utilized 8,565 single nucleotide polymorphisms and insertion/deletion markers distributed across the genome in genotype-by-sequencing for genetic diversity analyses. Phylogenetics, genetic population structure, and principal component analysis showed that a total of 110 varieties were classified into four distinct clusters according to different populations geographically and historically. Furthermore, the genome sequences of 19 rice varieties along with historic representations in Hokkaido, nucleotide diversity and FST values in each cluster revealed that artificial selection of elite phenotypes focused on chromosomal regions. These results clearly demonstrated the history of the selections on agronomic traits as genome sequences among current rice varieties from Hokkaido. 相似文献
97.
Tomoyasu Nishizawa Aihua Quan Ayaaki Kai Kanako Tago Satoshi Ishii Weishou Shen Kazuo Isobe Shigeto Otsuka Keishi Senoo 《Biology and Fertility of Soils》2014,50(6):1001-1007
Pelleted poultry manure is recommended for use with agricultural soil as a replacement for chemical fertilizers; however, application of the manure stimulates nitrous oxide (N2O) emission from the soil through denitrification. To mitigate the N2O emission caused by application of pelleted poultry manure, soil microcosms were set up; each microcosm was inoculated with one of the following N2-generating denitrifier strains previously been isolated from paddy soil: Azoarcus, Dyella, Dechloromonas, Niastella, and Burkholderia. When pelleted poultry manure was incubated on its own, N2O was produced by denitrification. In contrast, N2O emission was significantly lowered when the manure was inoculated with most of the N2-generating strains. In soil microcosms, N2O was emitted during incubation after application of the pelleted manure, while N2O flux was significantly lowered when the soil was inoculated with Azoarcus sp. KS11B, Niastella sp. KS31B, or Burkholderia sp. TSO47-3 on the 12th day of incubation. In addition, when pelleted manure was inoculated with the strains prior to application in the soil microcosms, the level of N2O emission was significantly lowered to ca. 40–60 % that from the non-inoculated control. Our study provides the prototype of a technique that uses microbial technology to mitigate N2O emission from agricultural soil fertilized with pelleted poultry manure. 相似文献
98.
Chondrulelike objects in short-period comet 81P/Wild 2 总被引：1，自引：0，他引：1
Nakamura T Noguchi T Tsuchiyama A Ushikubo T Kita NT Valley JW Zolensky ME Kakazu Y Sakamoto K Mashio E Uesugi K Nakano T 《Science (New York, N.Y.)》2008,321(5896):1664-1667
The Stardust spacecraft returned cometary samples that contain crystalline material, but the origin of the material is not yet well understood. We found four crystalline particles from comet 81P/Wild 2 that were apparently formed by flash-melting at a high temperature and are texturally, mineralogically, and compositionally similar to chondrules. Chondrules are submillimeter particles that dominate chondrites and are believed to have formed in the inner solar nebula. The comet particles show oxygen isotope compositions similar to chondrules in carbonaceous chondrites that compose the middle-to-outer asteroid belt. The presence of the chondrulelike objects in the comet suggests that chondrules have been transported out to the cold outer solar nebula and spread widely over the early solar system. 相似文献
99.
Takeshi Watanabe Hideo Aoki Kanako Watanabe Masashi Maita Yoichi Yamagata Shuichi Satoh 《Fisheries Science》2001,67(3):461-469
SUMMARY: Two feeding experiments were conducted to evaluate the feed quality of non-fish meal diets having the same protein ingredient composition but prepared as different types, and to determine the supplemental effect of crystalline essential amino acids (EAA) on feed utilization by young yellowtail, Seriola quinqueradiata . Non-fish meal diets formulated with soy protein concentrate, defatted soybean meal, corn gluten meal, meat meal, and krill meal were prepared as either soft dry pellets (SDP) or extruded pellets (EP) by using a large- or a small-sized twin screw extruder under different preparation conditions; or as a single moist pellet (SMP), each with and without EAA mixtures. Commercial yellowtail SDP was used as the control diet. Fish weighing 134 g and 237 g on average were reared with the experimental diets, for 93 (net cages) and 44 (aquariums) days, respectively. The fish fed both the control and test diets were found to have a good appetite. Growth rate and feed gain ratio were highest in the control diet group. The physiological condition of fish fed the control diet was evaluated as superior compared to those on the non-fish meal diets. Among the non-fish meal diet groups, the best performances were obtained for fish fed the SDP type diet with EAA supplement, and performance parameters excelled in the order of SDP, EP and SMP both among the diets with and without supplemental EAA. This suggests that the nutritional quality of non-fish meal diet was affected by the diet preparation method. It also indicates that supplementation of EAA could improve the quality of non-fish meal diets, irrespective of the diet type, probably as a result from the enhancement of feed protein utilization. 相似文献