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41.
Pantu Kumar Roy Ahmad Yar Qamar Xun Fang Ghangyong Kim Seonggyu Bang Mahanama De Zoysa Sang Tae Shin Jongki Cho 《Reproduction in domestic animals》2021,56(2):342-350
Oxidative stress is inevitable as it is derived from the handling, culturing, inherent metabolic activities and medium supplementation of embryos. This study was performed to investigate the protective effect of chitosan nanoparticles (CNPs) on oxidative damage in porcine oocytes. For this purpose, cumulus–oocyte complexes (COCs) derived from porcine slaughterhouse ovaries were exposed to different concentrations of CNPs (0, 10, 25 and 50 µg/ml) during in vitro maturation (IVM). Oocytes treated with 25 µg/ml CNPs showed significantly higher levels of GSH, along with a significant reduction in ROS levels compared to control, CNPs10 and CNPs50 groups. In parthenogenetic embryo production, the maturation rate was significantly higher in the CNPs25 group than that in the control and all other treated groups. In addition, when compared to the CNPs50 and control groups, CNPs25-treated oocytes showed significantly higher cleavage and blastocyst development rates. The highest concentration of CNPs reduced the total cell number and ratio of ICM: TE cells in parthenogenetic embryos, suggesting that there is a threshold where benefits are lost if exceeded. In cloned embryos, the CNPs25 group, as compared to all other treated groups, showed significantly higher maturation and cleavage rates. Furthermore, the blastocyst development rate in the CNPs25-treated group was significantly higher than that in the CNPs50-treated group, as was the total cell number. Moreover, we found that cloned embryos derived from the CNPs25-treated group showed significantly higher expression levels of Pou5f1, Dppa2, and Ndp52il genes, compared with those of the control and other treated groups. Our results demonstrated that 25 µg/ml CNPs treatment during IVM improves the developmental competence of porcine oocytes by reducing oxidative stress. 相似文献
42.
为掌握陕西省西安市牛羊养殖场职业暴露人群布鲁氏菌感染风险因素,做好高危人群布鲁氏菌病防控工作,2019年对西安市39个布鲁氏菌病阳性养殖场及346位职业暴露高危人群进行流行学调查,并采用单因素分析及多因素logistic回归分析,找出养殖场高危人群感染风险因素。结果显示:畜间布鲁氏菌病在养殖方式上主要发生于散养场(27/39),时间上发生于4月、7—8月、10月,在畜种上以羊群为主(28/39)。对从业人员主观潜在风险因素的单因素分析发现,感染从业人员与未感染高危人员在挤奶、喝生牛羊乳、接生、免疫、接触流产畜(流产物及粪便)病差异均有统计学意义(P 0.05);多因素logistic回顾分析发现,挤奶(OR=2.435,95%CI=1.137~5.071)、喝生牛羊乳(OR=2.893,95%CI=1.218~7.569)、接生(OR=3.217,95%CI=1.382~8.036)、接触流产畜(流产物及粪便,OR=2.439,95%CI=1.175~5.209)是从业人员感染的风险因素。同时,对养殖场客观潜在风险因素的单因素分析及多因素logistic回顾分析显示,场区消毒(OR=0.694,95%CI=0.307~1.043)、流产病死畜无害化处理(OR=0.549,95%CI=0.325~1.105)也是从业人员感染的风险因素。结果表明,挤奶、喝生牛羊乳、接生、接触流产畜(流产物及粪便)是从业人员感染的主观性风险因素,场区消毒、流产病死畜无害化处理是从业人员感染的客观风险因素。结果提示,牛羊养殖场及从业人员应加强对上述感染风险因素的控制,降低暴露风险。同时,相关机构应开展牛羊养殖场职业暴露人群布鲁氏菌病防控知识科普宣传,提升其防范能力。 相似文献
43.
Su Lai Yee Mon Moe Lwin Aye Aye Maw Lat Lat Htun Saw Bawm Kotaro Kawabe Yasuhiko Wada Shin Okamoto Takeshi Shimogiri 《Animal Science Journal》2021,92(1):e13647
Myanmar indigenous chickens play important roles in food, entertainment, and farm business for the people of Myanmar. In this study, complete mitochondrial D-loop sequences (1232 bp) were analyzed using 176 chickens, including three indigenous breeds, two fighting cock populations, and three indigenous populations to elucidate genetic diversity and accomplish a phylogenetic analysis of Myanmar indigenous chickens. The average haplotype and nucleotide diversities were 0.948 ± 0.009 and 0.00814 ± 0.00024, respectively, exhibiting high genetic diversity of Myanmar indigenous chickens. Sixty-four haplotypes were classified as seven haplogroups, with the majority being haplogroup F. The breeds and populations except Inbinwa had multiple maternal haplogroups, suggesting that they experienced no recent purifying selection and bottleneck events. All breeds and populations examined shared haplogroup F. When 232 sequences belonging to haplogroup F (79 from Myanmar and 153 deposited sequences from other Asian countries/region) were analyzed together, the highest genetic diversity was observed in Myanmar indigenous chickens. Furthermore, Myanmar indigenous chickens and red junglefowls were observed in the center of the star-like median-joining network of 37 F-haplotypes, suggesting that Myanmar is one of the origins of haplogroup F. These findings revealed the unique genetic characteristic of Myanmar indigenous chickens as important genetic resources. 相似文献
44.
Hyunseok Kim Min Jang Chi Won Shin Hyungjoo Han Jeesoo Cha Inhyung Lee Won-gyun Son 《Veterinary anaesthesia and analgesia》2021,48(1):92-100
ObjectiveTo describe an approach for ethmoidal nerve block (EBLOCK) and to compare the effects of a maxillary nerve block (MBLOCK), EBLOCK and their combination (M-EBLOCK) on heart rate (HR), systolic (SAP), mean (MAP), diastolic (DAP) arterial pressures and respiratory rate (fR) during nasal stimulation in dogs.Study designProspective, blinded, randomized, crossover placebo-controlled study.AnimalsBeagle dogs (five cadavers, nine live dogs), with a median (interquartile range) weight of 10.5 (10.3–11.0) kg.MethodsThe accuracy of iohexol injections (each 1 mL) at the maxillary and ethmoidal foramina in cadavers was evaluated using computed tomography. Then, anesthetized dogs were administered four bilateral treatments separated by 1 week, saline or 2% lidocaine 1 mL per injection: injections of saline at the maxillary and ethmoidal foramina (Control), injections of lidocaine at the maxillary foramina and saline at the ethmoidal foramina (MBLOCK), injections of saline at the maxillary foramina and lidocaine at the ethmoidal foramina (EBLOCK) and injections of lidocaine at all foramina (M-EBLOCK). The ventral nasal meatus was bilaterally stimulated using cotton swabs, and HR, SAP, MAP, DAP and fR were continuously recorded. Values for each variable were compared before and after stimulation using Wilcoxon signed-rank test. Changes in variables among treatments were analyzed using Mann–Whitney U and Kruskal–Wallis tests (p ≤ 0.05).ResultsComputed tomography revealed iohexol distribution around the openings of the target foramina in all cadavers. In living dogs, HR, SAP, MAP, DAP and fR significantly increased after stimulation within each treatment (p < 0.03). Physiologic responses were significantly attenuated, but not absent, in the M-EBLOCK [HR (p = 0.019), SAP, MAP, DAP and fR (all p ≤ 0.001)] compared with those in the Control.Conclusions and clinical relevanceConcurrent injections of lidocaine at the maxillary and ethmoidal foramina attenuated HR, arterial pressure and fR responses to nasal stimulation in Beagle dogs. 相似文献
45.
46.
Separation anxiety (SA) is a serious behavioral problem in dogs. In this study, salivary cortisol was studied to determine if the owner''s odor or voice could reduce SA in dogs. Twenty-eight dogs with SA were divided into three groups: group 1 (control), group 2 (with owner''s clothes during the separation period; SP) and group 3 (a recording of the owner''s voice was played during SP). The dog''s saliva was collected after the owner and their dog were in the experimental room for 5 min (PRE). The dog was then separated from the owner for 20 min and saliva collected four times at intervals of 5 min (SP1–4). Finally, the owner was allowed back into the room to calm the dog for 5 min, after which saliva was collected (POST). Evaluation of salivary cortisol concentrations by ELISA revealed that the ratios of SP1 concentration to PRE or POST concentrations were significantly higher in group 1 than in group 2 or 3. Additionally, the concentrations of SP1–PRE and SP1–POST among groups differed significantly. These findings indicate that the owner''s odor or voice may be helpful to managing stress in dogs with SA. 相似文献
47.
Yeon Soo Chung Jae Won Song Dae Ho Kim Sook Shin Young Kyung Park Soo Jin Yang Suk Kyung Lim Kun Taek Park Yong Ho Park 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(2):199-206
Limited information is available regarding horse-associated antimicrobial resistant (AR) Escherichia (E.) coli. This study was designed to evaluate the frequency and characterize the pattern of AR E. coli from healthy horse-associated samples. A total of 143 E. coli (4.6%) were isolated from 3,078 samples collected from three national racetracks and 14 private horse-riding courses in Korea. Thirty of the E. coli isolates (21%) showed antimicrobial resistance to at least one antimicrobial agent, and four of the AR E. coli (13.3%) were defined as multi-drug resistance. Most of the AR E. coli harbored AR genes corresponding to their antimicrobial resistance phenotypes. Four of the AR E. coli carried class 1 integrase gene (intI1), a gene associated with multi-drug resistance. Pulsed-field gel electrophoretic analysis showed no genetic relatedness among AR E. coli isolated from different facilities; however, cross-transmissions between horses or horses and environments were detected in two facilities. Although cross-transmission of AR E. coli in horses and their environments was generally low, our study suggests a risk of transmission of AR bacteria between horses and humans. Further studies are needed to evaluate the risk of possible transmission of horse-associated AR bacteria to human communities through horse riders and horse-care workers. 相似文献
48.
Jee-Hoon Lee Ik-Jae Kang A-Reum Kim You-Sun Noh Hee-Chun Chung Bong-Kyun Park 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(2):253-256
This study was conducted to determine if humoral antibody response of foot-and-mouth disease (FMD) vaccine improved in 8-week-old growing pigs born to well-vaccinated sows pre-treated with 60 mg of poly-γ-glutamic acid (γ-PGA) three days before vaccination. Antibody against FMD virus serotype O was measured 0, 2, 4 and 6 weeks post-vaccination, using a PrioCHECK FMDV type O ELISA kit. The results showed that positive antibody reactions against FMDV serotype O antigen among a component of the vaccine significantly increased in response to pre-injection with γ-PGA. 相似文献
49.
50.
Shin JH Sakoda Y Kim JH Ochiai K Umemura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(11):1167-1169
Rabbits were immunized with inactivated influenza virus via the subarachnoidal (SA) or subcutaneous (SC) route, and the antibody titers in cerebrospinal fluid (CSF) and serum were assayed. There were no nervous signs or morphological lesions related to SA immunization. In the SC group, the antibody titer was elevated in serum, but not elevated in CSF. In the SA group, the antibody titer was significantly elevated in serum and even in CSF, and their antibody titers were much greater than in the SC group. The present results suggest that intrathecal immunization is more effective than SC immunization at inducing a protective immune response against the transneural spread of viruses. 相似文献