Initial stage of cheese production: a molecular modeling study of bovine and camel chymosin complexed with peptides from the chymosin-sensitive region of κ-casein     

Sørensen J  Palmer DS  Qvist KB  Schiøtt B 《Journal of agricultural and food chemistry》2011,59(10):5636-5647

Bovine chymosin has long been the preferred enzyme used to coagulate cow's milk, in the initial stage of cheese production, during which it cleaves a specific bond in the milk protein κ-casein. Recently, camel chymosin has been shown to have a 70% higher clotting activity toward cow's milk and, moreover, to cleave κ-casein more selectively. Bovine chymosin, on the other hand, is a poor clotting agent toward camel's milk. This paper reports a molecular modeling study aimed at understanding this disparity, based on homology modeling and molecular dynamics simulations using peptide fragments of κ-casein from cow and camel in both bovine and camel chymosin. The results show that the complex between bovine chymosin and the fragment of camel κ-casein is indeed less stable in the binding pocket. The results also indicate that this in part may be due to charge repulsion between a lysine residue in bovine chymosin and an arginine residue in the P4 position of camel κ-casein.  相似文献   

Effects of storage atmosphere and heme state on the color and visible reflectance spectra of salmon ( Salmo salar ) fillets     

Ottestad S  Sørheim O  Heia K  Skaret J  Wold JP 《Journal of agricultural and food chemistry》2011,59(14):7825-7831

It has previously been observed that the color of mackerel muscle is dependent on the status of heme as myoglobin and hemoglobin and hence the storage atmosphere. This study gives strong indications of this being the case also in salmon. Three different storage conditions were used to promote the oxidized, reduced, and carbon monoxide (CO) bound forms of heme in salmon and mackerel fillets. Color determination (instrumental color analysis, imaging, and sensory evaluation) and spectroscopic measurements were performed to study how spectral changes corresponded to color variations. Storage in CO significantly increased the redness in mackerel. This was also seen in salmon to such a degree that it was visible over normal levels of salmon carotenoids. Air storage increased the yellowness and reduced the redness in mackerel, but this effect was partly concealed in salmon by the astaxanthin absorption. The spectral differences due to storage condition could be ascribed to the spectral features characterizing heme of different oxidation states and bound to different ligands. The status of heme should therefore always be considered when experiments related to salmon color are performed. The findings could help in the understanding, control, and prediction of color loss in salmon during processing, storage, and transport.  相似文献   

Recovery of infectious virus from full-length cowpox virus (CPXV) DNA cloned as a bacterial artificial chromosome (BAC)     

Roth SJ  Höper D  Beer M  Feineis S  Tischer BK  Osterrieder N 《Veterinary research》2011,42(1):3

ABSTRACT: Transmission from pet rats and cats to humans as well as severe infection in felids and other animal species have recently drawn increasing attention to cowpox virus (CPXV). We report the cloning of the entire genome of cowpox virus strain Brighton Red (BR) as a bacterial artificial chromosome (BAC) in Escherichia coli and the recovery of infectious virus from cloned DNA. Generation of a full-length CPXV DNA clone was achieved by first introducing a mini-F vector, which allows maintenance of large circular DNA in E. coli, into the thymidine kinase locus of CPXV by homologous recombination. Circular replication intermediates were then electroporated into E. coli DH10B cells. Upon successful establishment of the infectious BR clone, we modified the full-length clone such that recombination-mediated excision of bacterial sequences can occur upon transfection in eukaryotic cells. This self-excision of the bacterial replicon is made possible by a sequence duplication within mini-F sequences and allows recovery of recombinant virus progeny without remaining marker or vector sequences. The in vitro growth properties of viruses derived from both BAC clones were determined and found to be virtually indistinguishable from those of parental, wild-type BR. Finally, the complete genomic sequence of the infectious clone was determined and the cloned viral genome was shown to be identical to that of the parental virus. In summary, the generated infectious clone will greatly facilitate studies on individual genes and pathogenesis of CPXV. Moreover, the vector potential of CPXV can now be more systematically explored using this newly generated tool.  相似文献   

Swine infection with Trichinella spiralis: Comparative analysis of the mucosal intestinal and systemic immune responses     

Picherot M  Oswald IP  Cote M  Noeckler K  Le Guerhier F  Boireau P  Vallée I 《Veterinary parasitology》2007,143(2):122-130

The immune protective response developed by swine against Trichinella spiralis is not yet fully understood, particularly at the mucosal level. This study aimed to characterise intestinal immunity to T. spiralis by comparison with the systemic response in specifically pathogen-free pigs. For this purpose, the kinetics of cytokine and antibody production were assessed in the intestinal mucosa and serum of swine infected with T. spiralis for up to 60 days post-infection (dpi). An ex vivo model of jejunum mucosa culture was used to collect the supernatant as a source of antibodies (Abs). Mucosal antibodies were observed by Western blot from 15 dpi, while serum antibodies were expressed from 20 dpi. Both sources of antibodies initially recognized a 110 kDa protein, followed by the identification of 35, 43/46 and 55/59 kDa proteins. IgG1 and IgA antibodies were strongly expressed within the mucosa. The expression levels of Type 1 (IFN-gamma, IL-12), Type 2 (IL-4, IL-6), pro-inflammatory (TNF-alpha) and regulatory (IL-10, TGF-beta) cytokines were assessed by RT-PCR in the intestinal mucosa and spleen. Both IL-10 and IFN-gamma mRNA levels were increased in mucosa, whereas IL-6 and IL-12 mRNA were expressed in spleen. Taken together, these results demonstrated a mixed Type 1/Type 2 profile, the Type 2 profile being dominant in the mucosa.  相似文献   

Electrical anisotropy below slow- and fast-moving plates: paleoflow in the upper mantle?     

Bahr K  Simpson F 《Science (New York, N.Y.)》2002,295(5558):1270-1272

Upper mantle electrical conductivities can be explained by hydrogen diffusivity in hydrous olivine. Diffusivity enhances the conductivity of olivine anisotropically, making the a axis the most conductive of the three axes. Therefore, the hypothesis that plate motion induces lattice-preferred orientation of olivine can be tested with the use of long-period electromagnetic array measurements. Here, we compared electrical anisotropies below the slow-moving Fennoscandian and fast-moving Australian plates. The degree of olivine alignment is greater in the mantle below the Fennoscandian plate than below the Australian plate. This finding may indicate that convection rather than plate motion is the dominant deformation mechanism.  相似文献   

Metabolomic responses in grain,ear, and straw of winter wheat under increasing sulfur treatment     

Christian Zörb  Karl Hermann Mühling  Mario Hasler  Victoria Gödde  Karsten Niehaus  Dirk Becker  Christoph‐Martin Geilfus 《植物养料与土壤学杂志》2013,176(6):964-970

Only little is known about the effect of a varying sulfur (S) nutrition on the pattern of metabolites in different organs of the ears of winter wheat (Tritcum aestivum L.) at final maturity. More insights into the metabolome as influenced by increasing S‐fertilizer rates would, however, be of particular interest in order to unravel S‐dependent physiological processes related to grain filling in wheat. We have therefore investigated the effects of varying sulfur nutrition on metabolite composition and distribution in the organs of the wheat ear and vegetative organs at final maturity. Gas chromatography–mass spectrometry–based metabolite profiles revealed that S deficiency decreased the bulk of metabolites in the straw in favor of an increasing metabolite concentration in the husk, rachis, and grains. Surprisingly, only four out of 109 detectable metabolites, namely N‐acetyl glucosamine, lysine, ferulic acid, and β‐aminoisobutyric acid were most responsible for organ‐specific differences in the metabolite profiles. Under S‐deficient conditions, N‐acetyl‐glucosamine, lysine, and β‐aminoisobutyric were increasingly transferred from source tissues into the ears and grains.  相似文献   

Niacin supplementation induces type II to type I muscle fiber transition in skeletal muscle of sheep     

Muckta Khan  Aline Couturier  Johanna F Kubens  Erika Most  Frank-Christoph Mooren  Karsten Krüger  Robert Ringseis  Klaus Eder 《Acta veterinaria Scandinavica》2013,55(1):85

Background

It was recently shown that niacin supplementation counteracts the obesity-induced muscle fiber transition from oxidative type I to glycolytic type II and increases the number of type I fibers in skeletal muscle of obese Zucker rats. These effects were likely mediated by the induction of key regulators of fiber transition, PPARδ (encoded by PPARD), PGC-1α (encoded by PPARGC1A) and PGC-1β (encoded by PPARGC1B), leading to type II to type I fiber transition and upregulation of genes involved in oxidative metabolism. The aim of the present study was to investigate whether niacin administration also influences fiber distribution and the metabolic phenotype of different muscles [M. longissimus dorsi (LD), M. semimembranosus (SM), M. semitendinosus (ST)] in sheep as a model for ruminants. For this purpose, 16 male, 11 wk old Rhoen sheep were randomly allocated to two groups of 8 sheep each administered either no (control group) or 1 g niacin per day (niacin group) for 4 wk.

Results

After 4 wk, the percentage number of type I fibers in LD, SM and ST muscles was greater in the niacin group, whereas the percentage number of type II fibers was less in niacin group than in the control group (P < 0.05). The mRNA levels of PPARGC1A, PPARGC1B, and PPARD and the relative mRNA levels of genes involved in mitochondrial fatty acid uptake (CPT1B, SLC25A20), tricarboxylic acid cycle (SDHA), mitochondrial respiratory chain (COX5A, COX6A1), and angiogenesis (VEGFA) in LD, SM and ST muscles were greater (P < 0.05) or tended to be greater (P < 0.15) in the niacin group than in the control group.

Conclusions

The study shows that niacin supplementation induces muscle fiber transition from type II to type I, and thereby an oxidative metabolic phenotype of skeletal muscle in sheep as a model for ruminants. The enhanced capacity of skeletal muscle to utilize fatty acids in ruminants might be particularly useful during metabolic states in which fatty acids are excessively mobilized from adipose tissue, such as during the early lactating period in high producing cows.  相似文献   

Long-term decline in the abundance of leafhoppers and planthoppers (Auchenorrhyncha) in Central European protected dry grasslands     

Sebastian Schuch  Karsten Wesche  Matthias Schaefer 《Biological conservation》2012,149(1):75-83

Landscapes in Central Europe have changed considerably during the last five decades, while nature reserves have been less affected. However, there is growing concern that species richness and population size of animals in reserves may decrease even under protection.We performed a comparative study of the development in auchenorrhynchan communities of governmentally protected dry grasslands in Eastern Germany and tested whether reserves were effective in maintaining insect communities. The historical surveys are from 1963 to 1967. Between 2008 and 2010 we revisited 26 of the original sites and sampled leafhoppers and planthoppers by applying the same sampling technique as in the 1960s. Thus, we were able to perform a 40-year-comparison for auchenorrhynchan species richness and abundance. Comparisons capturing three successive years of each period allowed us to assess interannual variability in abundance.Species richness hardly differed between the two periods. However, some new species were found, and therefore species composition changed. Species abundance and overall numbers of individuals declined. Mainly species known to be very common dry grassland specialists exhibited strong declines in abundance. On average, only 27% of auchenorrhynchan numbers caught from 1964 to 1966 were recorded for the years 2008 to 2010.The results suggest that weather conditions and climate change are minor factors in the decline in auchenorrhynchan populations in recent years. Although the studied areas were under protection during the last 50 years, air-borne nitrogen deposition, the introduction of modern intense land use practices and alterations in plant communities, are likely to have influenced auchenorrhynchan abundance to a large extent.  相似文献   

Fifty years of change in Central European grassland vegetation: Large losses in species richness and animal-pollinated plants     

Karsten Wesche  Benjamin Krause  Heike Culmsee  Christoph Leuschner 《Biological conservation》2012,150(1):76-85

There is growing concern that biodiversity loss in European agricultural landscapes is having negative effects on functional trait diversity. Long-term studies examining vegetation changes from the period before agricultural industrialisation are however rare. Here, we ask how management intensification and increased nutrient input initiated in the 1950/1960s have altered grassland plant community composition, species diversity and functional trait composition using comprehensive datasets from five floodplain regions (plus one protected reference region) in northern Germany. Sites with available historical relevés and vegetation maps (1950/1960s, 1990s) were resampled in 2008 to facilitate the analysis of a period spanning four to five decades.Plant community composition changed tremendously in all study regions during the 50 year period, which was related to increasing Ellenberg indicator values for nutrient availability. Species richness at the plot-level fell by 30–50% over the period, and losses in functional diversity were equally large. A non-formal comparison with the results from the protected reference study region indicates that the changes may mostly be attributable to local nutrient input rather than to supra-regional climate change. Our results indicate a consistent trend towards much more species-poor communities dominated by mow-tolerant, N-demanding competitive grasses, whereas species with more ruderal strategies, species flowering early in the season and, in particular, insect-pollinated herbs have all decreased. The substantial loss of nectar-producing grassland herbs is likely to have negative effects on the abundance of pollinating insects, with consequences for the grassland animal communities. This highlights the growing need for adequate grassland management schemes with low N input to preserve high-nature-value grassland.  相似文献   

Preparation and comparative release characteristics of three anthocyanin encapsulation systems     

Oidtmann J  Schantz M  Mäder K  Baum M  Berg S  Betz M  Kulozik U  Leick S  Rehage H  Schwarz K  Richling E 《Journal of agricultural and food chemistry》2012,60(3):844-851

Bilberries (Vaccinium myrtillus L.) and their major polyphenolic constituents, anthocyanins, have preventive activities inter alia against colon cancer and inflammatory bowel diseases. However, anthocyanins are sensitive to environmental conditions; thus their bioavailability in the gastrointestinal tract is an important determinant of their in vivo activity. In the study reported here, the potential benefits of encapsulating an anthocyanin rich bilberry extract (BE) on anthocyanin stability were investigated. Nonencapsulated BE and three different BE loaded microcapsule systems were incubated in simulated gastric fluid (SGF) and fed state simulated intestinal fluid (FeSSIF). After exposure to these media, released anthocyanins were identified and quantified by HPLC with UV/Vis detection. Although a rapid release of anthocyanins was observed within the first 20 min, encapsulation of anthocyanins doubled the amount of available anthocyanins after 150 min of incubation. These results illustrate the ability of encapsulation to inhibit early degradation of anthocyanins in the intestinal system.  相似文献