EcPV-2 is transcriptionally active in equine SCC but only rarely detectable in swabs and semen from healthy horses     

Sykora S  Samek L  Schönthaler K  Palm F  Borzacchiello G  Aurich C  Brandt S 《Veterinary microbiology》2012,158(1-2):194-198

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Analysis of the proteins and peptides isolated from incubation medium of early equine conceptuses - preliminary results     

Sven Budik  Katharina Nöbauer  Ebrahim Razzazi-Fazeli  Christine Aurich 《Journal of Equine Veterinary Science》2012

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Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry     

KW Berendzen  M Böhmer  N Wallmeroth  S Peter  M Vesi  Y Zhou  FK Tiesler  F Schleifenbaum  K Harter 《Plant methods》2012,8(1):25-17

ABSTRACT: Understanding protein and gene function requires identifying interaction partners usingbiochemical, molecular or genetic tools. In plants, searching for novel protein-proteininteractions is limited to protein purification assays, heterologous in vivo systems such as the yeast-two-hybrid or mutant screens. Ideally one would be able to search for novel proteinpartners in living plant cells. We demonstrate that it is possible to screen for novel proteinproteininteractions from a random library in protoplasted Arabidopsis plant cells and recoversome of the interacting partners. Our screen is based on capturing the bi-molecularcomplementation of mYFP between an YN-bait fusion partner and a completely random preyYC-cDNA library with FACS. The candidate interactions were confirmed using in plantaBiFC assays and in planta FRET-FLIM assays. From this work, we show that the wellcharacterized protein Calcium Dependent Protein Kinase 3 (CPK3) interacts with APX3,HMGB5, ORP2A and a ricin B-related lectin domain containing protein At2g39050. This isone of the first random in planta screens to be successfully employed.  相似文献   

Ocular manifestations of a metastatic adenocarcinoma in a horse     

Franziska L. Matheis  Katharina Birkmann  Maja Ruetten  Simon A. Pot  Bernhard M. Spiess 《Veterinary ophthalmology》2013,16(3):214-218

A 10‐year‐old German Warmblood gelding was referred to the Equine Department of the Vetsuisse Faculty, University of Zurich, Switzerland, for an iris mass OD, lethargy, intermittent fever, and coughing. Ophthalmic examination revealed a 7 × 9 mm raised, fleshy, whitish to pinkish, vascularized iris mass at the 2 o`clock position OD. Fundic examination showed multifocal round, brown to black, slightly raised lesions with indistinct margins and a surrounding hyperreflective zone OU. Physical examination revealed a temperature of 39.2 °C, sinus tachycardia, preputial and ventral edema, and an enlarged right mandibular lymph node. Results of a complete blood count and plasma biochemical profile showed mild anemia, leukocytosis, and thrombocytopenia. Severe splenopathy, moderate splenomegaly, and severe pulmonary pathology with nodules and large areas of consolidated lung parenchyma were observed on abdominal ultrasound and thoracic radiographs, respectively. Fine needle aspirates of the enlarged mandibular lymph node showed malignant epithelial neoplastic cells. The horse was euthanized because of the poor prognosis and subsequently underwent postmortem examination. Macroscopic necropsy and histopathology revealed an adenocarcinoma of suspected pulmonary origin with involvement of eyes, heart, liver, kidneys, spleen, diaphragm, skeletal muscles, mandibular, pulmonary, and internal iliac lymph nodes. Metastatic adenocarcinoma should be considered as a differential diagnosis in horses with iris masses, multifocal chorioretinal infiltrates, and clinical signs that conform to a paraneoplastic syndrome.  相似文献   

Surveillance of H5 avian influenza virus in wild birds found dead   总被引：1，自引：0，他引：1  

Happold JR  Brunhart I  Schwermer H  Stärk KD 《Avian diseases》2008,52(1):100-105

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Quantitative,noninvasive assessment of intra‐ and extraocular perfusion by contrast‐enhanced ultrasonography and its clinical applicability in healthy dogs     

Klaas‐Ole Blohm  Katharina M. Hittmair  Alexander Tichy  Barbara Nell 《Veterinary ophthalmology》2019,22(6):767-777

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Contrast‐enhanced ultrasonography characteristics of intrathoracic mass lesions in 36 dogs and 24 cats     

Thorsten Rick  Miriam Kleiter  Ilse Schwendenwein  Eberhard Ludewig  Martin Reifinger  Katharina M. Hittmair 《Veterinary radiology & ultrasound》2019,60(1):56-64

Contrast‐enhanced ultrasonography (CEUS) is increasingly available for veterinary patients, however limited studies describe the use of this method for characterizing intrathoracic mass lesions. The aim of this prospective, observational study was to describe CEUS enhancement patterns for intrathoracic mass lesions in a sample of cats and dogs. Sixty patients (36 dogs, 24 cats) were included. Standardized CEUS examinations were performed for 41 pulmonary masses (68%) and 19 mediastinal masses (32%). Final diagnosis was based on cytology and/or histopathology. Absolute time to enhancement (TTE) values were recorded for the intrathoracic mass lesions and spleen. The spleen was used as a reference parenchymal organ to calculate relative TTE (rTTE) values. Absolute TTE of the spleen and intrathoracic mass lesions differed for dogs and cats (P = 0.001). The rTTE values significantly differed between lesions of neoplastic versus non‐neoplastic origin (P = 0.004). The majority of neoplastic pulmonary masses were supplied by bronchial arteries (63%), while most nonneoplastic pulmonary masses were supplied by pulmonary arteries (78%). The sensitivity and specificity for detecting pulmonary neoplastic masses with rTTE were 63% and 78%, respectively. Enhancement patterns for mediastinal thymomas and lymphomas significantly differed (P = 0.002). Thymomas enhanced heterogeneously in a centripetal pattern (86%), whereas lymphomas typically enhanced uniformly in a centrifugal pattern (75%). Findings indicated that CEUS is a feasible method for characterizing intrathoracic mass lesions in dogs and cats, however, the diagnostic sensitivity for detecting neoplastic pulmonary masses was low.  相似文献   

Randomized field trial on the effects of body weight and short transport on stress and immune variables in 2‐ to 4‐week‐old dairy calves     

Christien Masmeijer  Bert Devriendt  Tina Rogge  Katharina van Leenen  Lieze De Cremer  Bonny Van Ranst  Piet Deprez  Eric Cox  Bart Pardon 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(3):1514-1529

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Virucidal effects of various agents—including protease—against koi herpesvirus and viral haemorrhagic septicaemia virus     

Anette Amtmann  Ibrahim Ahmed  Petra Zahner-Rimmel  Adam Mletzko  Lisa Katharina Jordan  Martin Oberle  Helmut Wedekind  Jürgen Christian  Sven Michael Bergmann  Anna Maria Becker 《Journal of fish diseases》2020,43(2):185-195

In a search for alternative, environmentally friendly and effective disinfecting agents, a commercially available protease—Neutrase^®—was tested in this work for inactivation of koi herpesvirus (KHV) and of viral haemorrhagic septicaemia virus (VHSV). For comparison, the stability of these viral pathogens in similar configurations at various pH values and concentrations of peracetic acid or quicklime, typically used for disinfection, was tested. Therefore, virus suspensions were incubated with various concentrations of different agents for 24 hr and the titre of the remaining infectious particles was determined by virus titration. Furthermore, the treatment of both viruses, with the agents at concentrations that were previously appointed as effective, was also examined in the presence of solid material (quartz sand). All procedures investigated in this study, including the protease treatment, were able to reduce the titre of KHV and VHSV below the detection limit of the titration. Although further studies are necessary, this is the first report of the application of a protease for the inactivation of the selected fish pathogens, demonstrating the great potential of the latter for disinfection.  相似文献   

Machine vision smart sprayer for spot-application of agrochemical in wild blueberry fields     

Travis Esau  Qamar Zaman  Dominic Groulx  Aitazaz Farooque  Arnold Schumann  Young Chang 《Precision Agriculture》2018,19(4):770-788

An essential part of the wild blueberry cropping system is the proper management of agrochemical inputs including herbicides, fungicides and insecticides. A machine vision system was developed and mounted on the rear sprayer boom 0.18 m in front of the sprayer nozzles capable of targeting the agrochemical application on an as-needed basis. The three-point hitch mounted sprayer featured 27 nozzles over a 13.7 m boom width and a storage tank capacity of 1135 l. Nine digital color cameras continually take images in real-time while computer software processes the images in 0.15 s to determine the target locations where the nozzles open and spray at speeds up to 1.77 m s^?1. Two wild blueberry fields in central Nova Scotia were selected for smart sprayer performance testing with spot-application (SA) of agrochemical as compared to control and uniform application techniques. Chateau^® herbicide was applied in a field with an infestation of hair cap moss. Spray droplet comparison showed moss patches were properly targeted using the smart sprayer. SA provided the same coverage performance as compared to uniform on the moss targets with herbicide application savings of 78.5% using the smart sprayer. Harvestable yield results were similar for all application tracks. TruPhos Magnesium and ZincMax foliar fertilizers were tank mixed with Bravo^® and Proline^® fungicides and applied to compare the difference of SA, control and uniform application. Results showed SA of foliar fertilizer and fungicide led to less premature leaf drop and increased the blueberry stem height, number of branches, stem diameter and fruit buds. SA of foliar fertilizer and fungicide also increased the percent of healthy wild blueberry plants by 57.8% and the harvestable yield by 137.8%. Fungicide application savings using the smart sprayer for SA were 11.6%.  相似文献