GnRH treatment at CIDR insertion influences ovarian follicular dynamics in Japanese black cows     

Ando T  Kamimura S  Hamana K  Watanabe G  Taya K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(3):275-280

Ovarian follicular dynamics and estrous synchronization after Gonadotropin-releasing hormone (GnRH) treatment at Controlled Internal Drug Releasing device (CIDR) insertion were investigated in Japanese Black cows. CIDR was inserted for eight cows at 7 days after estrus. Cows were allocated to either Group A: 8-day CIDR insertion with GnRH treatment on d 0 (n=4, d 0=CIDR insertion) or Group B: 8-day CIDR insertion (n=4). Both groups were injected with prostaglandin F2alpha (PGF2alpha) on d 7. Ultrasonography and blood sampling were performed twice daily. Intensive sampling was performed every 15 min for 8 hr to determine the pulsatile release of LH on d -1, d 5 and d 10. Three of four cows showed intermediate ovulation within 2 days after GnRH treatment during CIDR insertion in Group A, whereas no ovulation was found in Group B. Three of four cows in Group A and all four cows in Group B ovulated after CIDR removal. Plasma progesterone concentrations from d 3 to d 7 in three intermediate ovulatory cows in Group A (8.4 +/- 1.6 ng/ml) was significantly higher than those in Group B (4.1 +/- 1.2 ng/ml; 4 cows) during CIDR insertion (P<0.01). Interval to estrus and ovulation after CIDR removal was observed at 60.0 +/- 12.0 hr and 76.0 +/- 6.9 hr in three cows in Group A, and 75.0 +/- 15.1 hr and 93.0 +/- 20.5 hr in Group B, respectively. There was a significant increase in LH pulse frequency on d 10 compared on d -1 or d 5 in both groups (P<0.05), in addition those on d 10 in Group A tended to be higher than in Group B. As a result, GnRH treatment at CIDR insertion at 7 days after estrus induced intermediate ovulation with formation of corpus luteum (CL) and rather synchronized emergence of ovulatory follicle during CIDR insertion. These induced CL increased plasma progesterone concentrations and contributed to precise synchronization.  相似文献   

A new liquid-type diet for leptocephali in mass production of artificial glass eels     

Yamada  Yoshiaki  Okamura  Akihiro  Mikawa  Naomi  Horie  Noriyuki  Tsukamoto  Katsumi 《Fisheries Science》2019,85(3):545-551

Fisheries Science - Development of artificial production of glass eels still presents many problems to be solved. What and how to feed larvae is one of the most crucial problems for commercial mass...  相似文献   

Lung Carcinogenic Bioassay of CuO and TiO(2) Nanoparticles with Intratracheal Instillation Using F344 Male Rats     

Yokohira M  Hashimoto N  Yamakawa K  Suzuki S  Saoo K  Kuno T  Imaida K 《Journal of toxicologic pathology》2009,22(1):71-78

Toxicity assessment of nanoparticles, now widespread in our environment, is an important issue. We have focused attention on the carcinogenic potential of copper oxide (CuO) and titanium dioxide (TiO(2)). In experiment 1, a sequential pilot study, the effectiveness of a carcinogenic bioassay featuring intraperitoneal injection (i.p.) of 20 mg 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) or 0.1% N-bis(2-hydroxypropyl)nitrosamine (DHPN) in drinking water for 2 weeks was examined. Based on the results, DHPN, as the lung carcinogen, and evaluation at week 30 were selected as the most appropriate for our purposes in Experiment 1. In experiment 2, the carcinogenic bioassay was used to assess the carcinogenic potentials of instilled nanoparticles of CuO and TiO(2). There were no significant intergroup differences in the lung neoplastic lesions induced by DHPN, although the neoplastic lesions induced by the nanoparticles in the CuO or TiO(2) intratracheal instillation (i.t.) groups, demonstrated a tendency to increase compared with the microparticles administration. At the very least, the carcinogenic bioassay with DHPN proved useful for assessment of the modifying effects of instilled particles, and further assessment of the carcinogenic potential of nanoparticles appears warranted.  相似文献   

Contribution of Amorphous Iron Compounds to Adsorptions of Pentavalent Antimony by Soils     

Tetsuya Takahashi  Katsumi Shozugawa  Motoyuki Matsuo 《Water, air, and soil pollution》2010,208(1-4):165-172

Antimony, a known toxic element, is emitted into the environment by industrial processes. The objective of this research was to identify the components adsorbing antimony(V) in soils. Antimony(V) uptake by soil samples and synthesized allophane was measured by the spiking method. The phosphorous sorption coefficient of soil samples was measured and the amorphous index calculated. The antimony(V) adsorptive capacity of various soils, calculated from the uptake, indicated that Andosol soils rich in amorphous components have a high capacity of adsorption. A strong relationship was observed between the amorphous index and antimony(V) adsorptive capacity and also between the Fe/(Al + Fe) ratio and antimony(V) adsorptive capacity of synthesized allophane, thus indicating that the contribution of iron-related surface base groups is higher than the aluminol and silanol groups. Evidence supporting this result was obtained from an extraction experiment in which 34–92% of antimony(V) adsorbed by soil samples was bound to amorphous iron compounds. This research indicates that the adsorption of antimony(V) is closer to that of arsenic(III) than arsenic(V), which is of interest in considering the mobility of antimony in the environment.  相似文献   

Rice bran fractions improve blood pressure, lipid profile, and glucose metabolism in stroke-prone spontaneously hypertensive rats     

Ardiansyah  Shirakawa H  Koseki T  Ohinata K  Hashizume K  Komai M 《Journal of agricultural and food chemistry》2006,54(5):1914-1920

Effect of dietary supplementation of two types of rice bran fraction on blood pressure (BP), lipid profile, and glucose metabolism in stroke-prone spontaneously hypertensive rats was studied. Male 4-week-old rats were divided into one group fed the AIN-93M-based control (C) diet and two groups fed diet supplemented with 60 g/kg of Driselase and ethanol fractions (DF and EF, respectively) of rice bran. After 8 weeks feeding, the BP decreased in the DF and EF groups in comparison with the C group (p < 0.01). Plasma ACE inhibitory activity, BUN, BUN/creatinine ratio, albumin, triglyceride, and glucose levels were lower in the DF and EF groups than in the C group (p < 0.01). Plasma nitric oxide and urinary 8-hydroxy-2'-deoxyguanosine levels were lower in the DF and EF groups than in the C group (p < 0.01). Rice bran fractions appear to have a beneficial dietary component that improves hypertension, hyperlipidemia, and hyperglycemia.  相似文献   

Antidiabetic activities of chalcones isolated from a Japanese Herb, Angelica keiskei   总被引：2，自引：0，他引：2  

Enoki T  Ohnogi H  Nagamine K  Kudo Y  Sugiyama K  Tanabe M  Kobayashi E  Sagawa H  Kato I 《Journal of agricultural and food chemistry》2007,55(15):6013-6017

Diabetes mellitus is a chronic disease that is characterized by hyperglycemia caused by insufficient insulin action. We have explored the edible ingredients from folk medicines in Japan that contain substances complementing insulin action, such as the induction of adipocyte differentiation and the enhancement of glucose uptake. We eventually found that the ethanol extract from a Japanese herb "Ashitaba", Angelica keiskei, contained two major chalcones of 4-hydroxyderricin (4-HD) and xanthoangelol that showed strong insulin-like activities via a pathway independent of the peroxisome proliferator-activated receptor-gamma activation. The 4-HD especially showed the preventive effects on the progression of diabetes in genetically diabetic KK-Ay mice.  相似文献   

Antiatherogenic effect of isoflavones in ovariectomized apolipoprotein e-deficient mice     

Sato M  Sato H  Ogawa A  Nomura R  Takashima S  Bang HJ  Matsuoka H  Imaizumi K 《Journal of agricultural and food chemistry》2007,55(22):8967-8971

The consumption of isoflavone-containing foods such as soybean and soybean products has been reported to have beneficial effects on the cardiovascular system in postmenopausal women. The present study was carried out to examine the mechanism underlying the beneficial effects of isoflavones in apolipoprotein (apo) E-deficient mice subjected to ovarian resection. Compared with sham-operated mice, ovariectomized mice had a larger arterial lesion area in the aortic root. Feeding the ovariectomized mice an isoflavone-containing diet (0.055 mg/kJ of total isoflavones/cal of diet) reduced the size of these lesions more than did feeding them with an isoflavone-free diet. Neither ovariectomy nor diet had a significant effect on the concentration of cholesterol in serum and urinary levels of isoprostanes, which are biomarkers for oxidative stress in vivo. The ovariectomized mice showed a greater increase in mRNA abundance for monocyte chemoattractant protein (MCP)-I in the aorta and in the level of nitric oxide (NO) secreted by peritoneal macrophages in culture than did the sham-operated mice. The isoflavone-containing diet lowered the MCP-I expression and the NO secretion more than did the isoflavone-free diet. These results suggest that dietary isoflavones confer an antiatherogenic effect by preventing the activation of macrophages due to the removal of ovaries.  相似文献   

Screening of inhibitory activities for elastase and collagenase in the edible parts of fish and shellfish     

Rio Yamauchi  Daikichi Mukouyama and  Katsumi Yamaguchi 《Fisheries Science》2000,66(4):798-800

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A multi-step inflow method for estimating hydraulic conductivity at low pressure under the wetting process   总被引：2，自引：0，他引：2  

Haruyuki Fujimaki  Mitsuhiro Inoue  Katsumi Konishi 《Geoderma》2004,120(3-4):177-185

The accurate determination of the wetting soil hydraulic properties for a wide range of water contents is essential for studying and predicting infiltration processes. We present a laboratory infiltration method for determining hydraulic conductivity function, K(θ), in the low-to-medium water content range. An initially air-dry soil core is subjected to infiltration from the bottom where the pressure head, ψ_bot, is controlled through a membrane. As soon as the wetting front arrives at the soil surface, the top 0.5-cm layer is sliced for measurement of the water content. The ψ_bot is stepwise increased as the hydraulic equilibrium is nearly attained at each step. The wetting water retention function, ψ(θ), is determined by curve-fitting the equilibrium inflow data and from the independently measured data obtained from vapor equilibrium. The parameter in the K(θ) is estimated inversely using the cumulative inflow and water content of the sliced layer. This method is verified through comparisons with K(θ) obtained by the Boltzmann transform method. Although requiring an additional operation, the slicing procedure is found to be valuable in enhancing the reliability of the optimized parameter. A sensitivity analysis shows that water vapor movement would be negligible under our experimental conditions.  相似文献   

Genetic variation in the mitochondrial and nuclear DNA of the Japanese conger Conger myriaster   总被引：1，自引：0，他引：1  

Satoshi Ishikawa  Yobuo Kimura  Tadashi Tokai  Katsumi Tsukamoto  Mutsumi Nishida 《Fisheries Science》2001,67(6):1081-1087

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