Amberjack <Emphasis Type="Italic">Seriola dumerili</Emphasis> interleukin-10 negatively suppresses host cell-mediated immunity     

Megumi Matsumoto  Mahumoud Tanekey Amer  Kyosuke Araki  Atsushi Nishitani  Kazuma Hayashi  Yutaka Takeuchi  Kazuhiro Shiozaki  Atsushi Yamamoto 《Fisheries Science》2018,84(5):857-867

Interleukin-10 (IL-10) is an anti-inflammatory cytokine and negatively regulates cell-mediated immunity (CMI) induction by inhibiting cytokine production in type 1 T helper cells. IL-10 genes have been isolated from several fish, and inflammatory cytokine inhibition by IL-10 has been well examined. However, a CMI regulator of IL-10 in fish has not yet been identified. In this study, we cloned the IL-10 gene in amberjack Seriola dumerili and analyzed its function using its recombinant protein (rIL-10). In an in vitro culture experiment, gene expression of inflammatory cytokines was suppressed in leukocytes incubated with rIL-10 compared with cells that only received Nocardia seriolae stimulation. This result suggests amberjack IL-10 has conserved function as an inflammatory cytokine inhibitor. Bactericidal activity of amberjack cells against intracellular pathogen stimulation was decreased in a rIL-10 dose-dependent manner. Furthermore, a significant reduction in the T-bet/GATA-3 ratio was observed in N. seriolae living cell (LC)?+?rIL-10-injected fish. Taken together, these results suggest amberjack rIL-10 suppresses CMI induction both in vitro and in vivo. In addition, the number of IgM⁺ cells among spleen leukocytes in N. seriolae?+?rIL-10-injected fish was higher than in only N. seriolae LC, suggesting that Th2-dominant immunity was induced by adding rIL-10.  相似文献   

Long‐term changes in recruitment of age‐0 Pacific bluefin tuna (Thunnus orientalis) and environmental conditions around Japan          下载免费PDF全文

Yukimasa Ishida  Hiromu Fukuda  Ko Fujioka  Osamu Sakai  Yuko Hiraoka  Kazuhiro Oshima  Shuya Nakatsuka  Nobuaki Suzuki  Hiroyuki Shimada 《Fisheries Oceanography》2018,27(1):41-48

Recruitment of age‐0 Pacific bluefin tuna (Thunnus orientalis) from 1952 to 2014 was examined by a sequential regime shift detection method. The regime shifts in recruitment were detected in 1957, 1972, 1980, 1994 and 2009. The durations of regime shift ranged from 8–15 years and averaged 13.0 years. In both the total (1952–2014) and data rich (1980–2014) periods, negative relationships were found between recruitment and the Pacific Decadal Oscillation in autumn, and positive relationships were found between recruitment and sea surface temperature (SST) anomalies in the northern part of the East China Sea, in the southwestern part of the Sea of Japan, and in the waters off Shikoku and Tokai in summer and autumn. The 1994 and 2009 regime shifts in recruitment occurred in the same years as shifts in SST anomalies in the northern part of the East China Sea in summer. These results suggest that the ocean conditions in the northern part of the East China Sea are closely related to recruitment of Pacific bluefin tuna, and that the warmer conditions result in higher recruitment of the species.  相似文献   

Effects of light quality on conidiophore formation of the melon powdery mildew pathogen <Emphasis Type="Italic">Podosphaera xanthii</Emphasis>     

Tomoko Suzuki  Shougo Nishimura  Kazuhiro Yagi  Ryousuke Nakamura  Yoshihiro Takikawa  Yoshinori Matsuda  Koji Kakutani  Teruo Nonomura 《Phytoparasitica》2018,46(1):31-43

The lengths of conidiophores in fungal colonies of the melon powdery mildew pathogen Podosphaera xanthii Pollacci KMP-6 N cultured under greenhouse (natural) conditions differed markedly from those cultured in a growth chamber. We hypothesized that light wavelength was responsible for the differences in conidiophore length. In this study, we examined the effects of light-emitting diode (LED) irradiation (purple, blue, green, orange, and red light) and white light on colony development and conidiophore formation in KMP-6 N using a stereomicroscope and a high-fidelity digital microscope. Colonies on leaves were flat under greenhouse conditions and under red LED light irradiation but were stacked under growth chamber conditions and under purple, blue, green, and orange LED light irradiation. In addition, KMP-6 N formed catenated conidia comprising six conidia per conidiophore under greenhouse conditions and red light but more than seven conidia per conidiophore under growth chamber conditions and purple, blue, green, and orange light. Furthermore, almost none of the conidia on top of the conidiophores grown under blue light were fully constricted. Therefore, these fungi could not scatter their conidia and spread infection. This is the first report of the effects of LED lights on conidiophore formation in the melon powdery mildew fungus P. xanthii. The results provide insight into the mechanisms underlying the responses of conidiophores to light of specific wavelengths and conidial scatter from conidiophores of melon powdery mildew fungi.  相似文献   

Breeding efforts to mitigate damage by heat stress to spikelet sterility and grain quality     

Tsutomu Ishimaru  Hideyuki Hirabayashi  Kazuhiro Sasaki  Changrong Ye  Asako Kobayashi 《Plant Production Science》2016,19(1):12-21

Global warming is predicted to aggravate the risk of unstable crop production. It is of great concern that damage to rice spikelet sterility and grain quality will increase, resulting in yield and economic losses. To secure the global food supply and farmers’ income, the development of rice cultivars with heat resilience is a pressing concern. Regarding spikelet sterility, rice cultivars with heat tolerance at different growth stages have been identified in recent years. The early-morning flowering (EMF) trait is effective in heat escape because it shifts the time of day of flowering to earlier in the morning when it is cooler. Although varietal differences are very small, there are some genetic resources for EMF in wild rice accessions. Regarding heat-induced grain chalkiness, heat-tolerant japonica cultivars for mitigating white-back type of chalky grains (WBCG) were found. Quantitative trait loci for heat tolerance at flowering, EMF, and for WBCG in grain quality have been mapped on the rice chromosomes. Further genetic efforts have been successfully connected to the development of near-isogenic lines for each trait with tagged molecular markers. These breeding materials are quite unique and useful in facilitating marker-assisted breeding toward the development of heat-resilient rice in terms of spikelet sterility and grain quality.  相似文献   

Bacterial wilt of three Curcuma species,C. longa (turmeric), C. aromatica (wild turmeric) and C. zedoaria (zedoary) caused by Ralstonia solanacearum in Japan     

Atsushi Ajitomi  Yasuhiro Inoue  Mitsuo Horita  Kazuhiro Nakaho 《Journal of General Plant Pathology》2015,81(4):315-319

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Protection induced by volatile limonene against anthracnose disease in Arabidopsis thaliana     

Kayoko Fujioka  Haruko Gotoh  Taku Noumi  Ami Yoshida  Yoshiteru Noutoshi  Yoshishige Inagaki  Mikihiro Yamamoto  Yuki Ichinose  Tomonori Shiraishi  Kazuhiro Toyoda 《Journal of General Plant Pathology》2015,81(6):415-419

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Factors maintaining species diversity in satoyama, a traditional agricultural landscape of Japan     

Kazuhiro Katoh  Sumire Sakai 《Biological conservation》2009,142(9):1930-26

Satoyama is a traditional agricultural landscape in Japan. It contains many kinds of organisms, including endangered species. Satoyama is composed of several habitat types, including paddy fields, secondary forests, secondary grasslands, ponds, and streams. High species richness has been sustained in most habitats by anthropogenic disturbances of intermediate impact, mainly activities related to agriculture. The variety of habitats and connectivity among them have contributed to the high species diversity within satoyama. These factors allow organisms to move among habitats and use different habitat types to obtain different resources. The connectivity among habitats is often more vulnerable than the specific habitats themselves under anthropogenic influences. In satoyama, species that require connectivity among habitats (e.g., grey-faced buzzard [Butastur indicus] and Genji firefly [Luciola cruciata]) have tended to decrease. The grey-faced buzzard is categorized as a “vulnerable” species in the Japanese Red List. It usually nests in forest trees and forages in habitats such as paddy fields, grasslands, and forests. Its foraging locations shift seasonally depending on food availability. It is thought that the degradation of either paddy fields or forests in a landscape may result in the disappearance of this species. The Genji firefly spends its larval period in stream water, pupates underground along streams, and flies near streams after emergence. This species requires not only the integrity of streams and nearby areas, but also connectivity between these areas. The ecology of these species suggests that the variety of habitats and connectivity among them are critical factors for their survival.  相似文献   

Effects of salt accumulation on the leaf water potential and transpiration rate of pot-grown wheat with a controlled saline groundwater table     

Kazuhiro NISHIDA  Nasir M. KHAN  Sho SHIOZAWA 《Soil Science and Plant Nutrition》2009,55(3):375-384

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Ceramide aminoethylphosphonate from jumbo flying squid Dosidicus gigas attenuates the toxicity of cyanotoxin microcystin-LR     

Masaharu Komatsu  Naoki Ichiyama  Takashi Kurimoto  Shota Takumi  Kazuhiro Shiozaki  Yasumasa Sugiyama  Tatsuhiko Furukawa  Seiichi Ando  Saki Itonori  Hiroaki Saito 《Fisheries Science》2013,79(2):313-320

We have previously established the method for isolation of ceramide aminoethylphosphonate (CAEP) from jumbo flying squid Dosidicus gigas. In this study, we performed a MTT assay to evaluate the safety of CAEP to the cell lines for the application to health food and supplements. The CAEP did not show any cytotoxicity to various HEK293-transfectant cells. Next, we elucidated the positive function of CAEP to the somatic cells. Recently, we have reported that hepatotoxin microcystin-LR was taken up into the hepatocytes mediated by hepatocellular uptake transporters OATP1B1 and OATP1B3, and the cells were induced cytotoxicity subsequently. Cytotoxicity of microcystin-LR to permanently OATP1B3-expressing HEK293-OATP1B3 cells rather than to HEK293-OATP1B1 cells was preferentially attenuated by CAEP in a concentration-dependent manner. In addition, the enzyme activity of serine/threonine phosphatase, which was inhibited by microcystin-LR, was recuperated by co-exposure to CAEP. Furthermore, microcystin-LR-induced cellular protein phosphorylation were disrupted by CAEP exposure. These results suggested that CAEP is a promising remedy and/or preventive medicine for liver damage with microcystin-LR.  相似文献   

First application of PCR-Luminex system for molecular diagnosis of fungicide resistance and species identification of fungal pathogens     

Hideo Ishii  Junko Tanoue  Michiyo Oshima  Wen-Hsin Chung  Kumiko Nishimura  Junichiro Yamaguchi  Fumihiro Nemoto  Kazuhiro So  Toshitaka Iwama  Hideaki Yoshimatsu  Motoshige Shimizu  Toru Kozawa 《Journal of General Plant Pathology》2008,74(6):409-416

Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that results in a replacement of valine with methionine at codon 75 of the fungicide target protein. PCR-Luminex, a novel system developed for high-throughput analysis of single nucleotide polymorphisms (SNPs) was successfully introduced to diagnose MBI-D resistance using specific oligonucleotide probes coupled with fluorescent beads. The PCR-Luminex system was further tested for its potential in identifying species causing Fusarium head blight on wheat. Four major pathogens, Fusarium graminearum (=F. asiaticum), F. culmorum, F. avenaceum, and Microdochium nivale, known to cause the disease, were tested, and the species were identified using the PCR-Luminex method. So far, this report is the first on the application of the DNA-based PCR-Luminex system in the area of crop protection and/or agricultural sciences.  相似文献