Genetic analysis of a null-allele for lipoxygenase-3 in rice seeds   总被引：2，自引：0，他引：2  

Yasuhiro Suzuki  Tsukasa Nagamine  Kazutoshi Okuno 《Euphytica》1996,91(1):99-101

Summary Lipoxygenase (LOX) catalyzes peroxidation of lipids in rice grains and is related to the formation of volatile compounds. We report on the mode of inheritance of LOX-3 in the rice variety Daw Dam. This variety lacks the LOX-3 (Suzuki et al., 1993), which is a major isozyme in rice seeds. The inheritance of LOX-3 in rice seeds was determined by crossing Daw Dam with rice cultivars having normal LOX-3 activity. The distribution of F₂ phenotypes for the presence and absence of LOX-3 from selfed F₁ plants fitted the ratio 3:1. Segregation ratio of the B₁F₁ generation gave a good fit to 1 presence of LOX-3: 1 absence. These data support the hypothesis that the lack of LOX-3 is recessive to the presence of LOX-3, and we conclude that the absence of LOX-3 is inherited as a single recessive trait. We discuss the possible use of the LOX-3 less mutation in relation to volatile compounds production in rice grains.Abbreviations mAb monoclonal antibody - LOX lipoxygenase - SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis  相似文献   

QTL mapping for yield and yield-contributing traits in sorghum (Sorghum bicolor (L.) Moench) with genome-based SSR markers     

Tariq Shehzad  Kazutoshi Okuno 《Euphytica》2015,203(1):17-31

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Characterization of Cu-resistant bacterial communities in Cu-contaminated soils     

Takashi Kunito  Kazunari Nagaoka  Nobuo Tada  Kazutoshi Saeki  Keishi Senoo  Hiroshi Oyaizu 《Soil Science and Plant Nutrition》2013,59(3):709-717

Bacterial communities in a spoil heap in a copper mine, a forest soil with high Cu contamination, and an agricultural soil with low Cu contamination were characterized in terms of Gram-staining, plasmid frequency, pigmentation, Cu-resistance system, and predominant Cu-resistant bacterial species. Similarity existed in that the ratio of green colonies for sorbing Cu and Gram-negative bacteria increased with the increase of the Cu content of the medium regardless of the characteristics of the bacterial communities. It was found that the plasmid was not present in most of the Cu-resistant bacteria of the soils. Curesistant genera including Burkholderia, Alcaligenes, and Methylobacterium species were isolated from the Cu contaminated soils using YG agar plates treated with 2 mM Cu. Furthermore, the bacterium with the highest Cu resistance (MIC = 5.5 mm Cu) was identified as a Gram-positive bacterium Bacillus sp., though most of the Cu-resistant bacteria were Gram-negative.  相似文献   

Functional degeneration of the resistance gene nsv against Melon necrotic spot virus at low temperature     

Kazutaka Kido  Tomofumi Mochizuki  Kazutoshi Matsuo  Chika Tanaka  Kenji Kubota  Takehiro Ohki  Shinya Tsuda 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(2):189-194

The single recessive gene, nsv, which confers resistance against Melon necrotic spot virus (MNSV), has recently been used to develop virus-resistant melon cultivars in Japan. However, the Chiba isolate of MNSV, a common isolate in Japan, infected resistant cultivars when inoculated melon plants were grown at 15°C. Viral RNAs accumulated in protoplasts from resistant cultivars at both 15 and 20°C. Mechanical inoculation of the cotyledons caused MNSV to spread throughout the leaves at 15°C, but not at 20°C. These results support our novel hypothesis that a temperature-sensitive inactivation of disease resistance genes occurs at the nsv locus in melon cultivars with the resistance gene grown at temperatures below 20°C. The first and second authors contributed equally to this research.  相似文献   

Construction of a linkage map and identification of DNA markers linked to Fom-1, a gene conferring resistance to Fusarium oxysporum f.sp. melonis race 2 in melon     

Takahiro Tezuka  Keisuke Waki  Kazutoshi Yashiro  Maki Kuzuya  Tomoko Ishikawa  Yasumasa Takatsu  Makoto Miyagi 《Euphytica》2009,168(2):177-188

Resistance to Fusarium oxysporum f.sp. melonis race 2 is conferred by a single dominant gene, Fom-1 in melon. Here, we identified DNA markers tightly linked to Fom-1 that could be used for marker assisted selection in breeding programs. First, we developed 125 F₂ plants derived from the cross between melon lines P11 (fom-1fom-1) and MR-1 (Fom-1Fom-1). Using the F₂ population, we constructed a linkage map including 14 SSR markers which had not been mapped previously. Fom-1 was confirmed to be allocated to linkage group 7. Then, we identified four AFLP markers using bulked segregant analysis. The AFLP marker TAG/GCC-470 was completely linked to Fom-1 and other three markers were mapped near Fom-1. TAG/GCC-470 and TCG/GGT-400 were respectively converted to STS and CAPS markers. Usefulness of DNA markers was confirmed in the analysis with several melon cultivars and lines.  相似文献   

Genetic diversity of Central Asian and north Caucasian Aegilops species as revealed by RAPD markers     

Kazutoshi Okuno  Kaoru Ebana  Bayarsukh Noov  Hisashi Yoshida 《Genetic Resources and Crop Evolution》1998,45(4):389-394

RAPD analysis of 112 accessions of Aegilops tauschii Coss. (genome DD), Ae. cylindrica Host (CCDD), Ae. crassa Boiss. (DDMM), Ae. biuncialis Vis. (UUMM) and Ae. triuncialis L. (UUCC) collected in the Central Asia and north Caucasia was conducted. Aegilops accessions were divided into two major groups, corresponding to the D genome species and the U genome species. These groups were also separated into sub-groups according to species, except for the Ae. tauschii-cylindrica complex of accessions from Central Asia. Aegilops tauschii from north Caucasia was divided into two varietal groups, tauschii and meyeri. The Central Asian accessions of Aegilops species were more diverse than the accessions from north Caucasia. Aegilops tauschii and Ae. cylindrica accessions from north Caucasia were genetically uniform. Associations between altitudal variation of Aegilops species and variability of RAPD markers were not found.  相似文献   

Healing process of the wounds of the branches of the Japanese persimmon that were caused by girdling,scoring, and strangulation     

Kazutoshi Hamada  Tsuneo OgataShinji Fujiwara  Kojiro Hasegawa 《Scientia Horticulturae》2009

Girdling is widely used for many crops, mainly in order to improve the fruit set, size, and quality and to increase the yield of flower buds. However, girdling may damage trees, and permanent injury can occur if callus bridges are not formed across the ring. We determined the regeneration of vascular bundles and the healing process of wounds caused by surgical treatments. It occurred as follows: necrosis of some cell layers on the edge of the cut, callus formation and dedifferentiation of the parenchyma cells, callus proliferation, contact between callus pads, callus bridge formation, and differentiation into the mature vascular bundle; however, the innermost callus cells remained undifferentiated. At 3 days after treatment (DAT), several phloem parenchyma cell layers showed symptoms of necrosis. At 10 DAT, callus formation started in the phloem tissue below the periderm. Initially, the callus cells were uniform; however, the callus tissue pad was distinguished into 2 layers based on color and firmness. After callus bridge formation, the inner callus differentiated into the mature xylem and the outer callus, into mature phloem. In strangulation, xylem elements were formed from the inner callus pad before callus bridge formation. The callus bridge formed within 6 weeks, 20–25 days, and 14 weeks after girdling, scoring, and strangulation, respectively.  相似文献   

Oncogenicity of infectious canine hepatitis virus in hamsters     

T Kinjo  R Yanagawa  Y Fujimoto 《The Japanese journal of veterinary research》1968,16(4):145-158

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A stable compound containing a silicon-silicon triple bond     

Sekiguchi A  Kinjo R  Ichinohe M 《Science (New York, N.Y.)》2004,305(5691):1755-1757

The reaction of 2,2,3,3-tetrabromo-1,1,4,4-tetrakis[bis(trimethylsilyl)methyl]-1,4-diisopropyltetrasilane with four equivalents of potassium graphite (KC8) in tetrahydrofuran produces 1,1,4,4-tetrakis[bis(trimethylsilyl)methyl]-1,4-diisopropyl-2-tetrasilyne, a stable compound with a silicon-silicon triple bond, which can be isolated as emerald green crystals stable up to 100 degrees C in the absence of air. The SiSi triple-bond length (and its estimated standard deviation) is 2.0622(9) angstroms, which shows half the magnitude of the bond shortening of alkynes compared with that of alkenes. Unlike alkynes, the substituents at the SiSi group are not arranged in a linear fashion, but are trans-bent with a bond angle of 137.44(4) degrees.  相似文献   

Effect of α-casein on DNA adsorption by Andosols and by soil components     

Kazutoshi?Saeki  Masao?Sakai  Takashi?KunitoEmail author 《Biology and Fertility of Soils》2012,48(4):469-474

Andosols and the soil components (allophanes, humic acids, and goethite) had been autoclaved to destroy the nuclease activity of soil microflora. DNA adsorption by allophanes and Andosols was decreased by increasing the amount of α-casein added to the allophanes and to soils up to casein concentration of 5 mg ml^?1. DNA adsorption by humic acids was significantly increased by increasing the amount of α-casein up to 1.0 mg ml^?1, whereas the addition of 20 mg α-casein ml^?1 completely blocked DNA adsorption. These results can explain why the addition of excess skim milk is operationally needed for effective DNA extraction from Andosols. The amount of DNA adsorbed by Andosols treated with dephosphorylated α-casein was significantly higher than that of not treated Andosols (p?相似文献