Contrast therapy: Tissue heating and cooling properties within the equine distal limb     

Kevin K. Haussler  Shana R. Wilde  Michael S. Davis  Ann M. Hess  C. Wayne McIlwraith 《Equine veterinary journal》2021,53(1):149-156

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Randomly primed,strand-switching,MinION-based sequencing for the detection and characterization of cultured RNA viruses     

Kelsey T. Young  Kevin K. Lahmers  Holly S. Sellers  David E. Stallknecht  Rebecca L. Poulson  Jerry T. Saliki  Stephen Mark Tompkins  Ian Padykula  Chris Siepker  Elizabeth W. Howerth  Michelle Todd  James B. Stanton 《Journal of veterinary diagnostic investigation》2021,33(2):202

RNA viruses rapidly mutate, which can result in increased virulence, increased escape from vaccine protection, and false-negative detection results. Targeted detection methods have a limited ability to detect unknown viruses and often provide insufficient data to detect coinfections or identify antigenic variants. Random, deep sequencing is a method that can more fully detect and characterize RNA viruses and is often coupled with molecular techniques or culture methods for viral enrichment. We tested viral culture coupled with third-generation sequencing for the ability to detect and characterize RNA viruses. Cultures of bovine viral diarrhea virus, canine distemper virus (CDV), epizootic hemorrhagic disease virus, infectious bronchitis virus, 2 influenza A viruses, and porcine respiratory and reproductive syndrome virus were sequenced on the MinION platform using a random, reverse primer in a strand-switching reaction, coupled with PCR-based barcoding. Reads were taxonomically classified and used for reference-based sequence building using a stock personal computer. This method accurately detected and identified complete coding sequence genomes with a minimum of 20× coverage depth for all 7 viruses, including a sample containing 2 viruses. Each lineage-typing region had at least 26× coverage depth for all viruses. Furthermore, analyzing the CDV sample through a pipeline devoid of CDV reference sequences modeled the ability of this protocol to detect unknown viruses. Our results show the ability of this technique to detect and characterize dsRNA, negative- and positive-sense ssRNA, and nonsegmented and segmented RNA viruses.  相似文献   

Humeral condylar fractures and fissures in the French bulldog     

Oliver J. Anderson BVetMed  Sorrel J. Langley-Hobbs MA  BVetMed  DSAS  DECVS  FHEA  Kevin J. Parsons BVSc  PhD  Cert SAS  Diplomate ECVS 《Veterinary surgery : VS》2023,52(1):134-145

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Management of yellow dwarf disease in Europe in a post‐neonicotinoid agriculture     

Louise Mc Namara  Kevin Gauthier  Lael Walsh  Gaël Thbaud  Michael Gaffney  Emmanuel Jacquot 《Pest management science》2020,76(7):2276-2285

Barley/cereal yellow dwarf viruses (YDVs) cause yellow dwarf disease (YDD), which is a continuous risk to cereals production worldwide. These viruses cause leaf yellowing and stunting, resulting in yield reductions of up to 80%. YDVs have been a consistent but low‐level problem in European cereal cultivation for the last three decades, mostly due to the availability of several effective insecticides (largely pyrethroids and more recently neonicotinoids) against aphid vectors. However, this has changed recently, with many insecticides being lost, culminating in a recent European Union (EU) regulation prohibiting outdoor use of the neonicotinoid‐insecticide compounds. This change is coupled with the growing challenge of insecticide‐resistant aphids, the lack of genetic resources against YDVs, and a knowledge deficit around the parameters responsible for the emergence and spread of YDD. This means that economic sustainability of cereal cultivation in several European countries including France and United Kingdom is now again threatened by this aphid‐vectored viral disease. In this review, we summarize the current knowledge on the YDV pathosystem, describe management options against YDD, analyse the impacts of the neonicotinoid ban in Europe, and consider future strategies to control YDV. © 2020 Society of Chemical Industry  相似文献   

Landscape drivers of connectivity for a forest rodent in a coffee agroecosystem     

Otero Jiménez  Beatriz  Li  Kevin  Tucker  Priscilla K. 《Landscape Ecology》2020,35(5):1249-1261

Landscape Ecology - The majority of remaining tropical forests exist as fragments embedded in a matrix of agricultural production. Understanding the effects of these agricultural landscapes on...  相似文献   

BUILDING CLIMATE-RESILIENT FOOD SYSTEMS IN EAST AND SOUTHEAST ASIA: VULNERABILITIES,RESPONSES AND FINANCING     

Yunyi ZHOU  Ziqi CHEN  Kevin Z. CHEN 《农业科学与工程前沿(英文版)》2023,10(1):16-30

Food system resilience to climate change is uniquely imperative for bringing Sustainable Development Goals within reach and leaving no one behind. Food systems in East and Southeast Asia are interacting with planetary boundaries and are adversely affected by extreme weather-related events. A practical question for East and Southeast Asian stakeholders is how to foster climateresilient food systems in the face of lingering food system vulnerabilities and policy gaps. This paper reviews food syste...  相似文献   

Breaking the ice: the introduction of biofouling organisms to Antarctica on vessel hulls          下载免费PDF全文

Kevin A. Hughes  Gail V. Ashton 《水产资源保护:海洋与淡水生态系统》2017,27(1):158-164

Settling velocity characterization of aquacultural solids   总被引：5，自引：0，他引：5  

Kevin B. Wong  Raul H. Piedrahita   《Aquacultural Engineering》2000,21(4):233-246

A top-loading settling column is described and used to characterize the settling properties of the solids in the discharge water from a commercial rainbow trout production facility. Mass-based and phosphorus-based settling curves are presented. The median settling velocity on a mass-basis for the settleable solids was 1.7 cm s⁻¹. The median settling velocity for the settleable phosphorus was 1.15 cm s⁻¹. Manually stripping fecal material from rainbow trout resulted in settleable solids with a median settling velocity of 0.7 cm s⁻¹. Examination of the settling velocity curves show that halving the overflow rate (OFR) from 2 to 1 cm s⁻¹ changes the removal efficiency from 0.61 to 0.73, an increase of about 20%. Halving the OFR again to 0.5 cm s⁻¹ increases the removal efficiency to 0.81, an improvement of about 11%. Settling characteristics of aquacultural solids will vary from facility to facility. The methods described in this paper can be used to perform a similar type of analysis at other aquacultural sites, which may be growing other species under different management regimes.  相似文献   

Tundra plants protect the soil surface from UV     

Kevin A. Hughes  Kerstin Scherer  Petra Rettberg  Pete Convey 《Soil biology & biochemistry》2006,38(6):1488-1490

In the Arctic, seasonal ozone depletion is resulting in periods of enhanced UV-B radiation at ground level while regional climate change is associated with increasing temperatures. These changes are likely to alter plant distribution, biodiversity and morphology, which may have knock-on effects for microbially driven biogeochemical cycling and other soil processes. Our study examined the transmission of solar UV radiation through arctic tundra plants using a portable UV radiometer and the DLR-biofilm biological UV dosimeter. A strong negative correlation was found between vegetation cover and UV transmission to the soil surface. Penetration of UV to the soil beneath tundra plants varied depending upon plant morphology, being greater through low creeping plants than cushion plants, grasses or mosses. UV transmission to the soil surface beyond the foliage edge also varied with plant morphology and the presence of flowers.  相似文献   

Finding a needle in the haystack: Regional analysis of suitable Eld’s deer (Cervus eldi) forest in Southeast Asia     

William J. McShea  Kevin Koy  Arlyne Johnson  Myint Aung 《Biological conservation》2005,125(1):101-111

For species whose decline preceded the modern era and whose distribution is in the developing world, it is difficult to map suitable habitat across its former range. Eld’s deer (Cervus eldi) is an endangered cervid whose range across Southeast Asia was reduced during the last century to disjoint populations in Myanmar and Cambodia. We used ecological data from the present populations to determine landscape and habitat parameters that would help us predict the occurrence of the species in forests not yet surveyed. The suitable-forest GIS model was created using four readily available datasets for elevation, forest type, canopy closure, and human density. Comparison of the GIS model with 24 verified sightings of Eld’s deer during recent large mammal surveys in Cambodia, found 22 sightings (92%) within predicted suitable forest. Use the suitable-forest GIS model to survey a province in southern Lao People’s Democratic Republic, located a single, previously unreported population from 9 patches surveyed. In a separate analysis, a logistic regression model to predict Eld’s deer habitat in Northern Cambodia found percent tree cover, presence of wetlands, and distance to villages as the best predictors of deer, similar to variables used in the GIS model, with the exception of the importance of wetlands. Using mean annual rainfall to rank suitable-forest patches identified in the GIS model indicated dry dipterocarp forests in Northeastern Cambodia and Northern Myanmar have the highest potential to conserve eld’s deer. Examination of the suitable-forest GIS map and current protected areas indicated only Cambodia, with 11% suitable forest protected, has placed sufficient dry dipterocarp forest under protected status. Other Southeast Asia countries have not recognized dry dipterocarp forest as a significant ecotype worthy of conservation status.  相似文献