EST-SSR based estimates on functional genetic variation in a barley (<Emphasis Type="Italic">Hordeum vulgare</Emphasis> L.) collection from Egypt     

Khaled F. M. Salem  Rajeev K. Varshney  Marion S. Röder  Andreas Börner 《Genetic Resources and Crop Evolution》2010,57(4):515-521

Genetic diversity was investigated in a set of 27 barley genotypes, originated from Egypt, using 23 expressed sequence tag (EST)-derived simple sequence repeats (eSSRs), representatives of the seven barley chromosomes. Ninety-five alleles were detected among all the barley accessions. For 22 polymorphic eSSRs, the number of alleles per locus varied from 2 to 12, with a mean of 4.318 alleles per locus. The lowest and the largest number of alleles per locus among the seven homeologous groups was observed in homeologous group 5H and 4H with 3.00 and 6.33, respectively. The gene diversity increased as the number of alleles increased. Gene diversity for 22 ESTs loci varied from 0.137 for GBM1404 to 0.896 for GBM1015 with an average of 0.563. A significant correlation coefficient between gene diversity and the number of alleles was high, r = 0.741 (P < 0.01). Cluster analysis was conducted based on eSSRs data to group the barley genotypes and to construct a dendrogram. Four groups can be distinguished by truncating the dendrogram at gs value of 0.77.  相似文献   

Pharmacological Studies of Artichoke Leaf Extract and Their Health Benefits     

Maryem Ben Salem  Hanen Affes  Kamilia Ksouda  Raouia Dhouibi  Zouheir Sahnoun  Serria Hammami  Khaled Mounir Zeghal 《Plant foods for human nutrition (Dordrecht, Netherlands)》2015,70(4):441-453

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Assessing genetic diversity of Egyptian hexaploid wheat (Triticum aestivum L.) using microsatellite markers     

Khaled F. M. Salem  Marion S. Röder  Andreas Börner 《Genetic Resources and Crop Evolution》2015,62(3):377-385

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Improved Salinity Tolerance by Phosphorus Fertilizer in Two Phaseolus vulgaris Recombinant Inbred Lines Contrasting in Their P‐Efficiency          下载免费PDF全文

A. Bargaz  R. M. A. Nassar  M. M. Rady  M. S. Gaballah  S. M. Thompson  M. Brestic  U. Schmidhalter  M. T. Abdelhamid 《Journal of Agronomy and Crop Science》2016,202(6):497-507

Legumes' sensitivity to salt is exacerbated under growth conditions requiring nitrogen fixation by the plant. Phosphorus (P) deficiency is widespread in legumes, especially common bean (Phaseolus vulgaris L). To examine the performance of P. vulgaris under salt stress conditions, a field experiment was conducted using two recombinants inbred lines (RILs) 115 (P‐deficiency tolerant) and 147 (P‐deficiency susceptible), grown under different salinity levels (L) (1.56, 4.78, and 8.83 dS m^?1 as LI, L2, and L3, respectively) and supplied with four P rates (0, 30, 60, and 90 kg ha^?1 P as P0, P30, P60, and P90, respectively) in order to assess the impact of P on salt tolerance. Results indicate that growing both RILs at P60 or P90 under all salinity levels (especially L1) significantly increased total chlorophyll, carotenoids, total soluble sugars, total free amino acids, and proline. Increasing P supply up to P60 under all salinity levels significantly induced higher accumulation of P, K⁺, Ca²⁺ and Mg²⁺ leaves in both RILs. Based on quadratic response over all locations, the maximum seed yield of 1.465 t ha^?1 could be obtained at application of P 81.0 kg ha‐1 in RIL115, while seed yield of 1.275 t ha^?1 could be obtained with P rate of 78.3 kg ha^?1 in RIL147. RIL115 exhibited more salt‐tolerance with positive consequence on plant biomass and grain yield stability. Improved salt tolerance through adequate P fertilization is likely a promising strategy to improve P. vulgaris salinity tolerance and thus productivity, a response that seems to be P‐rate dependent.  相似文献   

Morphogenetic responses of Populus alba L. under salt stress     

Mejda Abassi  Khaled Mguis  Zoubeir Béjaoui  Ali Albouchi 《林业研究》2014,25(1):155-161

The morphogenetic responses to salt stress of Tunisian Populus alba clones were studied in order to promote their plantation in damaged saline areas.One year-old plants of three P.alba clones(MA-104,MA-195 and OG)were subjected to progressive salt stress by irrigation during two consecutive years.The plants were grown in a nursery,inside plastic receptacles containing sandy soil and were irrigated with tap water(control)or 3–6 g/l NaCl solution.During this study,leaf epinasty,elongation rate,vigor,internode length,plant architecture,and number of buds were evaluated.Test clone response was highly dependent on the applied treatment and degree of accommodation.The most pronounced alterations were induced under 6g/l of NaCl treatment including leaf epinasty,leaf elongation rate delay,vigor decrease,internode length shortening,and morphogenetic modifications.These responses were less noticeable in the MA-104 clone with respect to the two other clones.The salt effect induced a delay in the leaf elongation rate on the MA-195 and OG clones leading to an early leaf maturity.The vigour and internode length of the MA-104 clone was less affected than the other clones.The OG clone was the most salt-sensitive thus,it developed shorter branches and more buds number than MA-195 and MA-104.The effect of long-term salt stress was to induce early flowering of the P.alba clones which suggests that mechanism of salt accommodation could be developed.  相似文献   

Evolution of Humic Fractions in Calcimagnesic Soils And Brown Isohumic Soils Amended With Compost and Manure     

Imen Ben Mahmoud  Mounir Medhioub  Hafedh Rigane  Khaled Medhioub 《Compost science & utilization》2013,21(4):253-256

The composting process of organic wastes consists of the bioconversion of biologically unstable wastes to stabilized products for application to soils. This study was carried out on different soils (brown isohumic soils in Sfax region in southern Tunisia and calcimagnesic soils in Korba region in northern Tunisia). The mineralization process of organic matter is more important in isohumic soils, showing less tenors of fine fractions than in calcimagnesic soils. The study of different humic fractions in amended soils shows that humic acid tenors and the polymerization rates are linked to soil nature. Calcimagnesic soils ensure a more important humification than isohumic soils.  相似文献   

Amelioration of sperm fertilizability,thyroid activity,oxidative stress,and inflammatory cytokines in rabbit bucks treated with phytogenic extracts     

Ibrahim T. El-Ratel  Wael M. Wafa  Hamdy A. El-Nagar  Abdelrhman M. Aboelmagd  Khaled H. El-Kholy 《Animal Science Journal》2021,92(1):e13560

This study investigated the beneficial effect of phytogenic extracts on semen quality, reproductive hormones, thyroid activity, immunity, hepatic antioxidant activity, and fertility in rabbit bucks. We divided 70 bucks into seven groups (10 in each). Group 1 was fed a basal diet (control); groups 2, 3, and 4 were fed the control diet with 30, 60, and 90 mg/kg of turmeric, respectively; and groups 5, 6, and 7 were fed the control diet with 50, 75, and 100 mg/kg of garlic extract, respectively, for 8 weeks. Rectal and skin temperatures decreased, while follicle-stimulating hormone, luteinizing hormone, triiodothyronine, thyroxine, testosterone, immunoglobulin M, tumor necrosis factor-alpha, and interleukin-6 in blood serum and glutathione peroxidase in the liver increased in all groups (p < .05). Garlic extract (100 mg/kg diet) increased adenosine triphosphate and glutathione in the liver tissues. All treatments significantly increased net semen volume, percentages of progressive motility, livability, curled tail, and intact acrosomes of spermatozoa, sperm cell concentration, and outputs of total and motile spermatozoa, while significantly decreased percentage of sperm abnormality. In conclusion, dietary supplementation of turmeric or garlic extract can be used as a suitable tool for enhancing the hepatic antioxidant activity, immunity, and semen quality in rabbit bucks.  相似文献   

Effect of Khat (Catha edulis Forsk) extract on testicular maturation in pre-pubertal and pubertal rats: A morphological and biochemical study     

Alaa S. Abou-Elhamd  Saud Sumayli  Klaus Steger  Asaad Khaled Mohamed Ali  Ahmed E. Zayed 《Anatomia, histologia, embryologia》2021,50(2):271-283

The present study aimed at analysing the effect of Khat plant extract on rat testicular development. Thirty-two weaned male albino rats (4 weeks old) were divided into four groups consisting of eight animals each. While control animals received normal saline, rats of groups I, II and III received 100, 200 and 300 mg Khat extract per kg body weight dissolved in distilled water by oral gavage daily for 8 weeks, respectively. Blood samples were collected in separate heparinized tubes by cardiac puncture from each rat and processed for measuring plasma levels of reproductive hormones LH, FSH, testosterone and prolactin. Five-µm sections were stained with haematoxylin and eosin and examined by light microscope. Some sections were immunostained for protamine-1 representing a biomarker for intact sperm differentiation. The present study clearly demonstrated that Khat extract has a pronounced effect on testicular maturation of developing albino rats at both the morphological and functional levels. Khat-treated groups revealed a significantly low serum testosterone level and severe impairment of spermatogenesis when compared with control animals. The current findings also verified, for the first time, that the final stages of sperm maturation (spermiogenesis) were strongly impaired after administration of Khat extract to experimental rats particularly at a higher dose (300 mg/kg body weight). This was proved by the very weak, if any, expression of protamine-1 in the maturing spermatids in Khat-treated rats.  相似文献   

Cassava, Manihot esculenta Crantz and wild relatives: Their relationships and evolution     

Nagib Nassar 《Genetic Resources and Crop Evolution》2001,48(5):429-436

About ninety eight species of Manihot arerecognized. All of them are native to the New world and areconcentrated in four regions of diversity in Brazil and CentralAmerica. All the Manihot species so far examinedhave 2n = 36 chromosomes. Interspecific hybrids betweencassava and its wild relatives show relatively regular meiosis, andfurther generations can be obtained. Electrophoresis shows affinityamong species of different sections, and between some of them andcassava. Both polyploidy and apomixis may have contributed tospeciation in this genus. Polyploidy offered genetic variabilitywhile apomixis is responsible for perpetuating new hybrid typesadapted to different environments. Cassava may have originated byhybridization between two wild Manihot speciesfollowed by vegetative reproduction of the hybrid.  相似文献   

Histomorphometric analysis of the irides of dogs, camels, buffalos and donkeys     

Aly KH  Abd-Elhafez E  Ali M  Abd-Elmaksoud A 《Research in veterinary science》2009,86(1):1-6

The present study was carried out to investigate the morphological and histomorphometrical characters of irides in dogs, camels, buffalos, and donkeys. The findings of the study revealed that, morphologically, the irides were consisted of an anterior border layer, a middle layer of connective tissue stroma and a posterior layer of pigmented epithelium. Interestingly, the anterior borders of all investigated animals were not enveloped by a distinct epithelial or endothelial lining, but on contrary, the posterior border was covered by pigmented epithelium. The constrictor and dilator iridial muscles were well developed in dogs, weakly developed in donkeys, and with an intermediate position in camels and buffalos. Morphometric analysis revealed significant species differences in the mean total thickness of the iris and its different layers. In addition significant differences were also found between the ratio of the means of different layers to the total thickness of the iris at the pupillary, middle and ciliary borders. In conclusion, these variations might be related to the different lifestyles and visual behaviour of the investigated animals.  相似文献