A rare case of uterine rupture and escape of conceptus into the abdominal cavity in a pre-parturient buffalo-cow     

D B Reddy  N Khan  G Yadgirkar 《The Veterinary record》1966,79(19):540-541

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Effects of plasmid-mediated growth hormone releasing hormone supplementation in young,healthy Beagle dogs     

Draghia-Akli R  Cummings KK  Khan AS  Brown PA  Carpenter RH 《Journal of animal science》2003,81(9):2301-2310

Our study focused on the evaluation of the pharmacological and toxicological effects of plasmid-mediated GHRH supplementation with electroporation in normal adult dogs over a 180-d period. Twenty-eight dogs (< 2 yr of age) were randomized to four groups. Three groups (four dogs/sex for each group) were treated with ascending doses of GHRH-expressing plasmid: 0.2, 0.6, and 1 mg. One group (two dogs of each sex) served as the control. Clinical observations and body weights were recorded. Hematological, serum biochemical, and urine analyses were performed. Serum IGF-I, ACTH, and insulin were determined. Necropsies were performed on d 93 and 180; organs were weighed and tissues were fixed and processed for light microscopy. Selected tissues were used to assess plasmid biodistribution on d 93. At all doses, plasmid GHRH caused increased weight gain (P < 0.001), without organomegaly. Serum glucose and insulin in fasted dogs remained within normal ranges at all time points. Adrenocorticotropic hormone was normal in all groups. Significant increases in number of red blood cells, hematocrit, and hemoglobin (P < 0.01) were observed. In conclusion, our study shows that plasmid-mediated GHRH supplementation is safe in electroporated doses up to 1.0 mg in young healthy dogs.  相似文献   

Construction and immunogenicity studies of recombinant fowl poxvirus containing the S1 gene of Massachusetts 41 strain of infectious bronchitis virus   总被引：9，自引：0，他引：9  

Wang X  Schnitzlein WM  Tripathy DN  Girshick T  Khan MI 《Avian diseases》2002,46(4):831-838

The spike 1 (S1) surface glycoprotein of infectious bronchitis virus (IBV) is the major inducer of the generation of virus neutralizing antibodies, and the administration of purified S1 has been shown to elicit a protective immune response against virulent virus challenge. On the basis of these observations, recombinant fowl poxvirus (rFPV) containing a cDNA copy of the S1 gene of IBV Mass 41 (rFPV-S1) was constructed and its immunogenicity and vaccine potential were evaluated. Initially, rFPV-S1 was shown to express the S1 in vito by indirect immunofluorescence staining and western blot analyses. Later, in vivo expression was demonstrated by the detection of IBV-specific serum immunoglobulin G and neutralization antibodies in the sera of chickens immunized with rFPV-S1. That the recombinant virus elicited anti-IBV protective immunity was indicated by the manifested, relatively mild clinical signs of disease, decreased titers of recovered challenge virus, and less severe histologic changes of the tracheas in virulent IBV Mass 41-challenged chickens previously receiving rFPV-S1 as compared with parental fowl poxvirus (FPV)-vaccinated control birds. In contrast, chickens immunized with either recombinant or parental FPV were resistant to a subsequent virulent FPV challenge. As to a preferred method of immunization, wing web administration appeared to be superior to the subcutaneous route because a greater percentage of birds vaccinated by the former protocol exhibited an anti-IBV humoral immune response. Thus, rFPV-S1 has potential as a poultry vaccine against both fowl pox and infectious bronchitis.  相似文献   

Diagnosis by polymerase chain reaction of Erysipelas septicemia in a flock of ring-necked pheasants     

Hennig GE  Goebel HD  Fabis JJ  Khan MI 《Avian diseases》2002,46(2):509-514

A flock of 810 pheasants experienced 6.2% mortality over 6 days. Affected birds were weak and lethargic for up to 24 hr before death. Examined birds were thin, and gross lesions consisted of thick opaque crops and cecal cores. Histologically, there was capillariasis of the crop and multifocal ulcerative typhlitis with Heterakis spp. infection, and numerous systemic intravascular monocytes were filled with clusters of blue rod-shaped organisms. The organisms were gram-positive bacilli by Brown and Brenn staining and ultrastructural analysis. Liver bacterial cultures were negative for pathogenic bacteria. Erysipelas septicemia was diagnosed by an Erysipelothrix species-specific polymerase chain reaction method with the substrate DNA isolated from formalin-fixed, paraffin-embedded liver.  相似文献   

Clinical syndrome associated with zolpidem ingestion in dogs: 33 cases (January 1998-July 2000)     

Richardson JA  Gwaltney-Brant SM  Albretsen JC  Khan SA  Porter JA 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(2):208-210

Zolpidem is a nonbenzodiazepine hypnotic of the imidazopyridine class that is used to treat insomnia in humans. Zolpidem binds selectively to the benzodiazepine omega-1 receptor and increases the frequency of chloride channel opening, which results in inhibition of neuronal excitation. A retrospective study was conducted of zolpidem ingestion in dogs that were reported to the ASPCA Animal Poison Control Center (APCC) between January 1998 and July 2000. Data analysis included amount ingested, clinical effects, and time of onset of signs. Thirty-three reports of zolpidem ingestion in dogs (ranging in age from 5 months to 16 years) were evaluated. Approximate ingested dosages ranged from 0.24 to 21 mg/kg. Clinical signs reported included ataxia (18 dogs; 54.5%), hyperactivity (10 dogs; 30.3%), vomiting (7 dogs; 21.2%), and lethargy (5 dogs; 15.2%), as well as panting, disorientation, nonspecific behavior disorder, and hypersalivation (4 dogs each sign; 12.1%). Other signs reported include tachycardia, tremors, apprehension, vocalization, hypersalivation, weakness, and hyperesthesia. In 85% percent of reports, clinical signs developed within 1 hour and usually resolved within 12 hours. Although central nervous system (CNS) depression is reported as a primary effect of zolpidem in humans and would also be expected in dogs, information obtained from this study indicates that some dogs may exhibit a paradoxical excitation reaction. This effect appears to vary among individual dogs.  相似文献   

Hybridization and in vitro culture of an orchid hybrid Ascocenda ‘Kangla’     

Rajkumar Kishor  P.S. Sha Valli Khan  G.J. Sharma 《Scientia Horticulturae》2006

The present study focuses on hybridization program involving two species belonging to two different vandaceous genera, viz., Ascocentrum ampullaceum (Roxb.) Schltr. var. auranticum, a narrow endemic orchid of Manipur and Vanda coerulea Griff., an endangered orchid of Appendix I of CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora), to synthesize the primary hybrid genus with intermediate and improved characters in the F₁ generation. Observations on the crossability in the present bigeneric cross between V. coerulea and A. auranticum had been achieved with 60% success when V. coerulea was taken as female parent. Murashige and Skoog (MS) basal medium at half-strength was effective for the development of the hybrid seedlings of V. coerulea × A. auranticum followed by Vacin and Went (VW) and Knudson C (KC) media. The best response of seedling growth was observed on MS medium at half-strength supplemented with 2.3 μM kinetin + 0.5 μM α-naphthalene acetic acid with maximum shoot height (2.7 cm), leaf number (4.6) and root number (4.1) after 150 days of inoculation. The survival percentage and growth performance of the seedlings were found to be higher (80% survival) in potting substrate consisting of brick:charcoal in the ratio 2:1 mulched with moss (Sphagnum sp.) than in potting substrate consisting of brick:charcoal:tree fern in the ratio 2:1:1. The first flowering was observed in the hybrid seedlings of V. coerulea × A. auranticum after 2 years of transfer to the ex vitro environment. Morphologically, the flowers differed from that of the parents clearly showing the success of the hybridization experiment. Registration of the hybrid has been made with the Royal Horticultural Society with the nomenclature Ascocenda ‘Kangla’ (No. T128725).  相似文献   

Comparative Disposition Kinetics of Albendazole in Sheep Following Oral and Intraruminal Administration     

Swarnkar CP  Sanyal PK  Singh D  Khan FA  Bhagwan PS 《Veterinary research communications》1998,22(8):545-551

The pharmacokinetics of albendazole was studied in sheep following single oral and intraruminal administration at nematocidal dose rates. The disposition curves of its metabolites indicated increased uptake of the drug in sheep following intraruminal as compared to oral dosing (p<0.05). The increased bioavailability of benzimidazole anthelmintics given by the intraruminal route could be exploited for optimizing the use of anthelmintic for sustained parasite control in small ruminants.  相似文献   

Effect of Gibberellic Acid Spray and Basal Nitrogen and Phosphorus on Productivity and Fatty Acid Composition of Rapeseed-Mustard     

N. A. Khan  H. R. Ansari  Samiullah 《Journal of Agronomy and Crop Science》1997,179(1):29-33

Two field experiments were conducted during] 994-95 to study the effect of spray of 10⁻⁵ M GA3 at 40 days after sowing on mustard ( Brassica juncea (L.) Czern & Coss.) cv. Varuna grown with basally applied 0, 40, 80 and 120 kg N ha⁻¹ (Expt. 1) and 0,15, 30 and 45 kg P ha⁻¹ (Expt. 2) on pod number per plant, seeds per pod, 1000 seed weight, seed yield, biological yield, harvest index and fatty acid composition of oil. No significant difference between water and GA3 spray was found when basally applied nitrogen was 0 or 40 kg N ha⁻¹. N₈₀ proved to be the best for yield characteristics. In another experiment on phosphorus, GA₃ and 30 kg P ha⁻¹ individually enhanced the yield, but interaction of GA₃ and P remained non-significant. The fatty acid composition of oil in both experiments was significantly affected only by nitrogen and phosphorus treatments for oleic acid and erucic acid. It was found that return in the form of yield was more for every kg applied fertilizer under GA, spray treatment. The response was more for N fertilizer in comparison to P. GA₃ at a low level of fertilization significantly increased the return from fertilization.  相似文献   

Glycinebetaine Improves Chilling Tolerance in Hybrid Maize     

M. Farooq  T. Aziz  M. Hussain  H. Rehman  K. Jabran  & M. B. Khan 《Journal of Agronomy and Crop Science》2008,194(2):152-160

Plant growth and development is hampered by various environmental stresses including chilling. We investigated the possibility of improving chilling tolerance in hybrid maize by glycinebetaine (GB) seed treatments. Maize hybrid (Hycorn 8288) seeds were soaked in 50, 100 and 150 mg l^?1 (p.p.m.) aerated solution of GB for 24 h and were dried back. Treated and untreated seeds were sown at 27 °C (optimal temperature) and at 15 °C (chilling stress) under controlled conditions. Germination and seedling growth was significantly hindered under chilling stress. Moreover, chilling stress significantly reduced the starch metabolism and relative water contents (RWC), and increased the membrane electrolyte leakage. However, activities of antioxidants (catalase, superoxide dismutase and ascorbate peroxidase) were increased under stress conditions. Seed treatments with GB improved the germination rate, root and shoot length, seedling fresh and dry weights, leaf and root scores, RWC, soluble sugars, α‐amylase activity and antioxidants significantly compared with untreated seeds under optimal and stress conditions. Induction of chilling tolerance was attributed to maintenance of high tissue water contents, reduced membrane electrolyte leakage, and higher antioxidant activities and carbohydrate metabolism. Seed treatment with 100 mg l^?1 GB was the best treatment for improving the performance of hybrid maize under normal and stress conditions compared with control and other levels used.  相似文献   

Urinary incontinence in a bitch caused by vaginal fibroma     

P N Sahay  L L Dass  A A Khan  K K Singh 《The Veterinary record》1985,116(3):76-77

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