Detection methods for biotech cotton MON 15985 and MON 88913 by PCR     

Lee SH  Kim JK  Yi BY 《Journal of agricultural and food chemistry》2007,55(9):3351-3357

Plants derived through agricultural biotechnology, or genetically modified organisms (GMOs), may affect human health and ecological environment. A living GMO is also called a living modified organism (LMO). Biotech cotton is a GMO in food or feed and also an LMO in the environment. Recently, two varieties of biotech cotton, MON 15985 and MON 88913, were developed by Monsanto Co. The detection method is an essential element for the GMO labeling system or LMO management of biotech plants. In this paper, two primer pairs and probes were designed for specific amplification of 116 and 120 bp PCR products from MON 15985 and MON 88913, respectively, with no amplification from any other biotech cotton. Limits of detection of the qualitative method were all 0.05% for MON 15985 and MON 88913. The quantitative method was developed using a TaqMan real-time PCR. A synthetic plasmid, as a reference molecule, was constructed from a taxon-specific DNA sequence of cotton and two construct-specific DNA sequences of MON 15985 and MON 88913. The quantitative method was validated using six samples that contained levels of biotech cotton mixed with conventional cotton ranging from 0.1 to 10.0%. As a result, the biases from the true value and the relative deviations were all within the range of +/-20%. Limits of quantitation of the quantitative method were all 0.1%. Consequently, it is reported that the proposed detection methods were applicable for qualitative and quantitative analyses for biotech cotton MON 15985 and MON 88913.  相似文献   

Hapten syntheses and antibody generation for a new herbicide, metamifop   总被引：1，自引：0，他引：1  

Moon JK  Keum YS  Hwang EC  Park BS  Chang HR  Li QX  Kim JH 《Journal of agricultural and food chemistry》2007,55(14):5416-5422

To develop a competitive indirect enzyme-linked immunosorbent assay for metamifop, a new aryloxyphenoxypropionic acid herbicide, three structurally related haptens were synthesized. Hapten conjugates to keyhole limpet hemocyanin and bovine serum albumin were used as immunogens and plate-coating antigens, respectively. Various sets of polyclonal antibodies from rabbits and the coating antigens were screened for the assay in simple homologous and heterologous ELISA formats. A selected heterologous ELISA was optimized to show an average IC50 value as low as 20.1 ng/mL, detection ranges of 1.0-350 ng/mL, and a lowest detection limit of 0.1 ng/mL. The cross-reactivities of other aryloxyphenoxypropionic acid herbicides to the antibodies were less than 0.5% in the assays except fenoxaprop-P and fenoxaprop-P ethyl, having a diaryl ether group identical to that of metamifop. Molecular modeling studies revealed that the physicochemical properties of the diaryl ether group are the most important determinants of sensitivity and selectivity. The results strongly indicate that the selected set of ELISA is a highly sensitive and convenient tool for detecting metamifop.  相似文献   

Eating out of home and its correlates in 10 European countries. The European Prospective Investigation into Cancer and Nutrition (EPIC) study     

Orfanos P  Naska A  Trichopoulos D  Slimani N  Ferrari P  van Bakel M  Deharveng G  Overvad K  Tjønneland A  Halkjaer J  Santucci de Magistris M  Tumino R  Pala V  Sacerdote C  Masala G  Skeie G  Engeset D  Lund E  Jakszyn P  Barricarte A  Chirlaque MD  Martinez-Garcia C  Amiano P  Quirós JR  Bingham S  Welch A  Spencer EA  Key TJ  Rohrmann S  Linseisen J  Ray J  Boeing H  Peeters PH  Bueno-de-Mesquita HB  Ocke M  Johansson I  Johansson G  Berglund G  Manjer J  Boutron-Ruault MC  Touvier M  Clavel-Chapelon F  Trichopoulou A 《Public health nutrition》2007,10(12):1515-1525

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Dietary patterns and survival of older Europeans: the EPIC-Elderly Study (European Prospective Investigation into Cancer and Nutrition)     

Bamia C  Trichopoulos D  Ferrari P  Overvad K  Bjerregaard L  Tjønneland A  Halkjaer J  Clavel-Chapelon F  Kesse E  Boutron-Ruault MC  Boffetta P  Nagel G  Linseisen J  Boeing H  Hoffmann K  Kasapa C  Orfanou A  Travezea C  Slimani N  Norat T  Palli D  Pala V  Panico S  Tumino R  Sacerdote C  Bueno-de-Mesquita HB  Waijers PM  Peeters PH  van der Schouw YT  Berenguer A  Martinez-Garcia C  Navarro C  Barricarte A  Dorronsoro M  Berglund G  Wirfält E  Johansson I  Johansson G  Bingham S  Khaw KT  Spencer EA  Key T  Riboli E  Trichopoulou A 《Public health nutrition》2007,10(6):590-598

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Biological evaluation of neopeptins isolated from a Streptomyces strain     

Kim YS  Kim HM  Chang C  Hwang IC  Oh H  Ahn JS  Kim KD  Hwang BK  Kim BS 《Pest management science》2007,63(12):1208-1214

BACKGROUND: Microbial secondary metabolites are a rich source of antifungal agents and have merit as alternatives to synthetic fungicides. To develop disease control agents against powdery mildew, the lipopeptide antibiotic neopeptins were identified from the culture broth of a Streptomyces sp., and in vivo control efficacy of the compounds was evaluated on cucumber plants under glasshouse conditions. RESULTS: The Streptomyces sp. KNF2047 antagonistic against powdery mildew development in cucumber plants was isolated from a soil sample. Antifungal compounds were purified from the culture broth and identified as neopeptin A and B. In vitro microtitre assays revealed the inhibitory activities of the compounds in the range 128-512 microg mL(-1) against the mycelial growth of Alternaria mali, Botrytis cinerea, Cladosporium cucumerinum, Colletotrichum lagenarium, Didimella bryoniae and Magnaporthe grisea. Although neither compound showed remarkable in vitro antifungal activity against other plant pathogenic fungi, a mixture of neopeptins (484 mg of neopeptin A and 290 mg of neopeptin B per gram of partially purified powder) showed potent protective and curative activity against cucumber powdery mildew in vivo. The disease control activity of the neopeptins at a concentration of 2.4 mg L(-1) was 92.1%, which was similar to that of the commercial fungicide fenarimol (89.3% at 63 mg L(-1)) and that of the commercial biocontrol agent Actinovate (67.4% at 2 x 10(7) cfu L(-1)). CONCLUSION: Neopeptin mixtures isolated from Streptomyces sp. KNF2047 showed potent disease control activity against powdery mildew development on cucumber plants. .  相似文献   

Isolation and antifungal activity of lignans from Myristica fragrans against various plant pathogenic fungi     

Cho JY  Choi GJ  Son SW  Jang KS  Lim HK  Lee SO  Sung ND  Cho KY  Kim JC 《Pest management science》2007,63(9):935-940

BACKGROUND: In a search for plant extracts with potent in vivo antifungal activity against various plant diseases, we found that treatment with a methanol extract of Myristica fragrans Houttyn (nutmeg) seeds reduced the development of various plant diseases. The objectives of the present study were to isolate and determine antifungal substances from My. fragrans and to evaluate their antifungal activities. RESULTS: Three antifungal lignans were isolated from the methanol extract of My. fragrans seeds and identified as erythro-austrobailignan-6 (EA6), meso-dihydroguaiaretic acid (MDA) and nectandrin-B (NB). In vitro antimicrobial activity of the three lignans varied according to compound and target species. Alternaria alternata, Colletotrichum coccodes, C. gloeosporioides, Magnaporthe grisea, Agrobacterium tumefaciens, Acidovorax konjaci and Burkholderia glumae were relatively sensitive to the three lignans. In vivo, all three compounds effectively suppressed the development of rice blast and wheat leaf rust. In addition, EA6 and NB were highly active against the development of barley powdery mildew and tomato late blight, respectively. Both MDA and NB also moderately inhibited the development of rice sheath blight. CONCLUSION: This is the first study to demonstrate the in vitro and in vivo antifungal activities of the three lignans from My. fragrans against plant pathogenic fungi.  相似文献   

Novel life stage targets against plum curculio, Conotrachelus nenuphar (Herbst), in apple integrated pest management     

Wise JC  Kim K  Hoffmann EJ  Vandervoort C  Gökçe A  Whalon ME 《Pest management science》2007,63(8):737-742

In this study the authors employed the plant-insect-chemistry (PIC) triad to investigate two novel life stage targets against the plum curculio (PC), Conotrachelus nenuphar (Herbst), in apple integrated pest management (IPM). Laboratory treated apple bioassays were used to determine if the insect growth regulator (IGR) insecticides novaluron and tebufenozide have physiological effects on PC larvae following adult exposure. Curative activity bioassays were conducted for IGR, neonicotinoid, oxidiazine and organophosphate insecticides on PC larvae post-infestation, and fruit penetration profiles of insecticides were developed. The results revealed that novaluron exhibits activity on PC larvae via vertical transmission following exposure of mated females to treated substrate. Surface treatments of azinphos-methyl, thiacloprid and thiamethoxam to preinfested fruit resulted in significant reductions in larval emergence. For all compounds, 50% or more of the total recovered active ingredient was from apple skin, and for azinphos-methyl, indoxacarb and thiamethoxam it was greater than 80%. For azinphos-methyl, novaluron, methoxyfenozide and thiacloprid, however, active ingredient was recovered from every section of the apple, from skin to core. Implications for twenty-first century IPM are discussed.  相似文献   

Photoinduced generation of 2,3-butanedione from riboflavin     

Jung MY  Oh YS  Kim DK  Kim HJ  Min DB 《Journal of agricultural and food chemistry》2007,55(1):170-174

The volatile compound formation from riboflavin solution of a phosphate buffer (0.1 M, pH 6.5) under light for 15 h was studied by SPME-GC and SPME-GC/MS analysis. Only one major compound in the riboflavin solution was formed and increased as the light exposure time increased. The light-exposed riboflavin solution had a buttery odor. The compound of riboflavin solution under light was analyzed by gas chromatography and olfactometry. The major volatile compound eluted from the gas chromatograph had a buttery odor. The buttery odor compound was positively identified as 2,3-butanedione by a combination of gas chromatographic retention time, mass spectrum, and odor evaluation of authentic 2,3-butanedione. The addition of sodium azide, a singlet oxygen quencher, to riboflavin solution minimized the formation of the buttery odor compound. Singlet oxygen was involved in the formation of the buttery odor. The 2,3-butanedione was produced from the reaction between riboflavin and singlet oxygen. Singlet oxygen was formed from triplet oxygen by riboflavin photosensitization mechanism. This is the first reported oxidation reaction between riboflavin and singlet or triplet in food and biological systems.  相似文献   

Evaluation of enhanced hygroscopicity, bifidogenicity, and anticariogenicity of enzymatically synthesized beta-galactosyl-trehalose oligosaccharides     

Ryu SI  Kim BG  Park MS  Lee YB  Lee SB 《Journal of agricultural and food chemistry》2007,55(10):4184-4188

beta-Galactosyl-trehalose oligosaccharides (beta-GTOs) were enzymatically prepared as a mixture of 6-beta-galactosyl-trehalose (1) and 4-beta-galactosyl-trehalose (2) with a 9:1 ratio (w/w). The beta-GTO mixture showed a highly enhanced hygroscopicity as compared to those of trehalose and other sugars used. At 72 h of incubation under 90% relative humidity and room temperature, it had a large increase in weight due to its moisture absorption, which was five times larger than that of trehalose, 1.9 times larger than that of sucrose, and 1.5 times larger than that of maltotriose. It was very effective in the growth promotion of Bifidobacteria, such as Bifidobacterium longum and Bifidobacterium bifidum, which was better than the growth promotion in the cases of trehalose and galactooligosaccharide. It also showed a highly anticariogenic property; it had only 10% cell proliferation of Streptococcus sobrinus for that of the sucrose control and 60% inhibition of insoluble glucan synthesis. Its effectiveness of inhibition was two and 1.5 times better than that of trehalose and one and two times than xylitol, respectively, against cell growth and glucan synthesis. Conclusively, the functionality of the beta-GTO in terms of hygroscopicity, bifidogenicity, and anticariogenicity was considerably improved as compared to that of trehalose. It is thus suggested that the beta-GTO might be applied as an effective humectant and prebiotic substitute with enhanced noncariogenicity in food applications.  相似文献   

Simple purification (desalting) procedure to facilitate structural analysis of an alkali-solubilized/neutralized starch solution by intermediate-pressure size-exclusion chromatography     

Kim HS  Huber KC 《Journal of agricultural and food chemistry》2007,55(13):4944-4948

A technique was established to remove impurities (e.g., salts) from starch dissolved in strong alkali and neutralized with acid to accommodate starch structural analysis via intermediate-pressure size-exclusion chromatography (IPSEC). Starch (corn and wheat) subjected to an alkaline-microwave dissolution scheme (35 s microwave heating in a mixture of 6 M urea and 1 M KOH) was either treated with ion-exchange resin or passed through a desalting column to remove salt/urea contaminants. Control (untreated) starch solution analyzed by IPSEC displayed a significant interfering peak (attributable to salt/urea), which coeluted with the starch amylose peak. The interfering peak was most efficiently eliminated by first passing the starch solution through a desalting column, which process effectively removed impurities (e.g., salts/urea) without appearing to adversely impact the starch structural analysis. This simple technique coupled with the rapid alkaline-microwave starch dissolution procedure greatly expedites structural investigation of starch by facilitating analysis by IPSEC.  相似文献