The influence of barley malt protein modification on beer foam stability and their relationship to the barley dimeric alpha-amylase inhibitor-I (BDAI-I) as a possible foam-promoting protein   总被引：2，自引：0，他引：2  

Okada Y  Iimure T  Takoi K  Kaneko T  Kihara M  Hayashi K  Ito K  Sato K  Takeda K 《Journal of agricultural and food chemistry》2008,56(4):1458-1464

The foam stability of beer is one of the important key factors in evaluating the quality of beer. The purpose of this study was to investigate the relationship between the level of malt modification (degradation of protein, starch, and so on) and the beer foam stability. This was achieved by examining foam-promoting proteins using two-dimensional gel electrophoresis (2DE). We found that the foam stability of beer samples brewed from the barley malts of cultivars B and C decreased as the level of malt modification increased; however, the foam stability of cultivar A did not change. To identify the property providing the increased foam stability of cultivar A, we analyzed beer proteins using 2DE. We analyzed three fractions that could contain beer foam-promoting proteins, namely, beer whole proteins, salt-precipitated proteins, and the proteins concentrated from beer foam. As a result, we found that in cultivar A, some protein spots did not change in any of these three protein fractions even when the level of malt modification increased, although the corresponding protein spots in cultivars B and C decreased. We analyzed these protein spots by peptide mass finger printing using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. As a result, all of these spots were identified as barley dimeric alpha-amylase inhibitor-I (BDAI-I). These results suggest that BDAI-I is an important contributor to beer foam stability.  相似文献   

外源人绒毛膜促性腺激素诱导黑边石斑鱼排卵的研究     

徐文刚  唐永政  张建柏  征矢野清  邹华锋 《广东农业科学》2020,47(4):122-128

【目的】了解外源人绒毛膜促性腺激素(human chorionic gonadotropin,HCG)诱导黑边石斑鱼(Epinephelus fasciatus)排卵的影响。【方法】以成熟的黑边石斑鱼为研究对象,测量其体长、体质量和肥满度后,在2018年7—8月,使用HCG激素(剂量为200 IU/kg或500 IU/kg)注射分别对6尾雌鱼进行成熟诱导。【结果】在200 IU/kg HCG处理中,注射24 h前有5尾雌鱼的卵母细胞处于第三次卵黄球前期阶段;注射48 h后2尾雌鱼出现核移动卵母细胞,1尾雌鱼的卵母细胞为第三次卵黄球后期阶段;注射60 h后1尾雌鱼出现排卵现象,其余5尾雌鱼均为第三次卵黄球后期阶段。在200 IU/kg HCG处理的排卵个体中,注射时其卵母细胞直径为477.0 μm,注射后60 h卵母细胞的直径增加至624.4 μm。此外,经催产、排卵、受精和孵化后共获得总卵数16 906粒,受精率为68.7%,孵化率为43.0%。而500 IU/kg HCG处理的黑边石斑鱼排卵失败。【结论】使用剂量为200 IU/kg的外源HCG激素可诱导黑边石斑鱼成熟且排卵,但为了提高排卵率、受精率和孵化率,其注射剂量和效应时间还需要进一步调节。  相似文献   

Screening for Small Molecule Inhibitors of BMP-Induced Osteoblastic Differentiation from Indonesian Marine Invertebrates     

Hiroyuki Yamazaki  Satoshi Ohte  Henki Rotinsulu  Defny S. Wewengkang  Deiske A. Sumilat  Delfly B. Abdjul  Wilmar Maarisit  Magie M. Kapojos  Michio Namikoshi  Takenobu Katagiri  Hiroshi Tomoda  Ryuji Uchida 《Marine drugs》2020,18(12)

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Species identification method for <Emphasis Type="Italic">Scombrops boops</Emphasis> and <Emphasis Type="Italic">Scombrops gilberti</Emphasis> based on polymerase chain reaction-restriction fragment length polymorphism analysis of mitochondrial DNA     

Shiro Itoi  Noriyuki Takai  Satomi Naya  Keitaro Dairiki  Akira Yamada  Seiji Akimoto  Kiyoshi Yoshihara  Haruo Sugita 《Fisheries Science》2008,74(3):503-510

ABSTRACT:   Gnomefish Scombrops boops and Scombrops gilberti are commercially important fishes in Japan, but these species are often confused in the markets because of their morphological similarity. To identify these two species, we performed nucleotide sequencing and restriction fragment length polymorphism (RFLP) analysis on 16S ribosomal RNA (rRNA) gene and the control region in mitochondrial DNA. Five and 12 nucleotide substitutions were observed between species in the 777-bp 16S rRNA gene and 471-bp control region, respectively. Diagnostic restriction sites for discriminating between S. boops and S. gilberti were found in the 16S rRNA gene, but not in the control region. Polymerase chain reaction (PCR)–RFLP analysis using two enzymes, Eco NI and Mva I, clearly discriminated between S. boops and S. gilberti identified by meristic characters. The PCR–RFLP analysis identified most of the 168 Scombrops young caught in the coastal waters of the Izu and Miura peninsulas as S. boops , suggesting that S. gilberti juveniles are rare in this area.  相似文献   

Nocturnal/diurnal demand-feeding pattern of yellowtail Seriola quinqueradiata under different keeping conditions   总被引：1，自引：0，他引：1  

Jun Kohbara  Iwao Hidaka  Isao Kuriyama  Mitsushi Yamashita  Masasuke Ichikawa  Kiyoshi Furukawa  Katsumi Aida  F. Javier Sánchez-Vázquez and  Mitsuo Tabata 《Fisheries Science》2000,66(5):955-962

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Investigations on the radioactive contamination of crop plants as a result of H-bomb detonation (Part 2) Root and foliage uptake of “Bikini ash”     

Shingo Mitsui  Sueo Aso  Kiyoshi Tensho  Kikuo Kumazawa 《Soil Science and Plant Nutrition》2013,59(1):17-18

The Foliar Uptake by Squash Plant

The radioactive ash for experimental use, hereafter referred to as “Bikini ash”, was prepared by igniting the heavily contaminated substances on board No. 5 Fukuryu Maru at about 650°C, followed by sifting through a 100 mesh sieve. On ignition some parts of the fission products, particularly iodine, ruthenium and tellurium would have possibly been lost to the air.  相似文献   

Cutting-grafts as a means to propagate greenhouse roses     

Kiyoshi Ohkawa 《Scientia Horticulturae》1980,13(2):191-199

A propagation method for rose nursery plants was studied by using a 2-node scion tongue-grafted on to an unrooted stock cutting. Uniform and disease-free nursery plants could be produced efficiently in a short period all the year round.Under greenhouse production trials, such cutting-grafts of ‘Sonia’ on R. multiflora ‘K-1’, R. indica ‘Major’, R. ‘Manetti’ and R. wichuraiana produced flowers of the same number and quality as when budded on to R. multiflora seedling root stock.  相似文献   

Trophinin is expressed in the porcine endometrium during the estrous cycle     

Nakano S  Kishi H  Ogawa H  Yasue H  Okano A  Okuda K 《The Journal of reproduction and development》2003,49(2):127-134

We investigated endometrial expression of trophinin mRNA and protein, homophilic cell adhesion molecules, during the estrous cycle of gilts. An immunopositive reaction for trophinin was observed in the luminal and glandular epithelia of the endometrium at all stages of the estrous cycle, but not in endometrial stromal cells or the myometrium. A partial coding sequence of porcine trophinin was similar to sequences in humans and mice, with homologies of 75% and 70%, respectively. As in humans and mice, the trophinin gene is expressed in the endometrium. Trophinin, however, is expressed in the endometrium of the pig throughout the estrous cycle, higher expression levels were observed at some points of the luteal phase, as in humans. These findings suggest that regulation of trophinin gene expression in the pig is different from that in mice, but similar to that in humans. Furthermore, the present results suggest that the pig might be a suitable model for studying the physiological importance of trophinin in early pregnancy in humans.  相似文献   

Localization of inhibin alpha-, betaA- and betaB-subunits and aromatase in ovarian follicles with granulosa theca cell tumor (GTCT) in 6 mares     

Hoque S  Derar RI  Senba H  Osawa T  Kano K  Taya K  Miyake Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):713-717

To clarify the morphological and immunohistochemical characteristics in mares with granulosa theca cell tumor (GTCT), the localization of inhibin subunits (alpha, betaA, betaB) and aromatase in the granulosa cell layers and theca layers in the ovarian follicles were determined by immunohistochemical staining. The follicles were obtained from the ovaries of 6 mares with GTCT and 4 normal mares as controls. Immunohistochemically, inhibin alpha-subunit was localized in the granulosa cells of all follicles showing different sizes in all GTCT cases and betaA- subunit was localized in two GTCT cases in all sized follicles. But inhibin betaB- subunit and aromatase were not localized in GTCT cases. On the other hand, inhibin alpha-, betaA-, and betaB-subunits and aromatase were localized in the large and medium sized follicles, but inhibin betaA- and betaB-subunits and aromatase were not stained in the small sized follicles in normal cases. These findings suggest that some mares with GTCT can secrete dimeric inhibin (inhibin A), but all GTCT cases cannot secrete inhibin B. By the results of aromatase staining it is clear that testosterone is not converted into estradiol due to the lack of aromatase in the GTCT follicles.  相似文献   

Flow cytometric analysis of chicken NK activity and its use on the effect of restraint stress     

Kushima K  Fujita M  Shigeta A  Horiuchi H  Matsuda H  Furusawa S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):995-1000

Immune system is organized by the influence of both neural and endocrine systems. NK activity plays an important role in the innate immunity. In this study, we observed the effects of restraint stress on chicken peripheral blood NK activity. Viability of FITC-labeled RP9 was measured with PI after treatment with the effector cells. Chicken peripheral blood CD8alpha+ cells expressed strong cytotoxic activity, in contrast to thrombocytes, while peripheral blood CD3+ CD8alpha+ cells and CD4+ cells had little cytotoxic activity. Con A supernatant enhanced the cytotoxic activity of CD8alpha+ cells. Therefore, it is considered that these cytotoxic activities measured by flow cytometry (FCM) analysis are NK activity. When chickens were exposed to restraint stress, the levels of serum corticosterone increased transiently over a short period of time while the NK activity decreased. The decreased NK activity, however, did not recover to the intact levels for a long time, even once the serum corticosterone levels had recovered. These data indicate that chicken NK activity is able to be measured by flow cytometric analysis and that restraint stress causes severe damage to the chicken NK activity.  相似文献