Characterization of polymorphisms of transferrin receptor and their association with susceptibility to ETEC F4ab/ac in pigs     

Y. Wang  J. Ren  L. Lan  X. Yan  X. Huang  Q. Peng  H. Tang  B. Zhang  H. Ji  & L. Huang 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2007,124(4):225-229

Diarrhoea caused by enterotoxigenic Escherichia coli (ETEC) expressing F4 (F4ab, F4ac and F4ad) fimbriae is a significant cause of mortality and morbidity in newborn and weaned pigs. The locus controlling susceptibility towards ETEC F4ab/ac has been mapped to SSC13q41, in which TFRC (transferrin receptor) was localized and considered as a positional candidate gene for ETEC F4ab/ac receptor. In this study, we determined susceptibility/resistance to ETEC F4ab/ac in a total of 755 F2 animals from a White Duroc x Erhualian intercross using a microscopic enterocyte adhesion assay. We identified two TFRC polymorphisms (SNPs 591 A>G and 632 A>G) in a single exon after comparative sequencing analysis of 2371-bp amplicons containing the complete coding region of TFRC using RNA of eight full-sib F2 animals with susceptible and resistant phenotypes. The intron sequences flanking the two exon polymorphisms were obtained, revealing an intron polymorphism (SNP 291 C>T). We genotyped the 19 founder animals of the White Duroc x Erhualian intercross for the identified polymorphisms, showing that only the 291 C>T polymorphism is a highly informative marker. We further genotyped all 59 F1 and 755 F2 animals for the 291 C>T polymorphism, and the association of this polymorphism with susceptibility/resistance to ETEC F4ab/ac in these F2 animals was evaluated by the transmission disequilibrium test. The result showed that the 291 C>T polymorphism is not a causal mutation, however, has a significant linkage disequilibrium with the ETEC F4ab/ac, especially F4ac receptor locus.  相似文献   

“十一五”我国饲料业发展的战略转变     

孔平涛 《中国畜牧杂志》2006,42(16):3-7

2006年7月26—27日,农业部在山东济南召开了全国饲料工作会议。会议总结了“十五”期间我国饲料业取得的成绩,分析了饲料业发展面临的形势与任务,明确了“十一五”饲料业的发展战略,部署了“十一五”政府的工作重点。本文即对此次会议要点进行了概括总结,以期引导读者了解相关政策并在今后工作中把握宏观方向,有所裨益。  相似文献   

禽流感抗原快速检测试纸条的研究   总被引：3，自引：1，他引：3  

沈浩龙  张长弓  黄印尧  金颜辉  余谦  孔繁德  庄雪梅 《福建畜牧兽医》2006,28(6):11-13

应用胶体金标记技术(Immunogold Labelling Technique)与免疫层析技术,在玻璃纤维膜和硝酸纤维膜的检测线(T)和对照线(C)上分别喷上胶体金AIV单克隆抗体(Ab2)偶联物和AIV单克隆抗体(Ab2)-羊抗鼠IgG多克隆抗体,制成禽流感抗原检测试纸条。用该试纸检测“AIV琼扩抗原”、“AIV H5N1抗原”、“AIV H7N1抗原”、“AIV H9N1抗原”均显阳性,而对禽源性的其它病毒抗原均显阴性;本试纸与韩国产“禽流感检测试纸条”同时检测相同的样品,结果完全一致,且具有很好的线性(敏感度),是一种微量、特异、快速、简便和结果容易判定的新的检测方法,可作为检测机构和养禽场检测初筛禽流感时使用。  相似文献   

犬附红细胞体感染诊治1例     

朱文静  邢增桦  孔陈葛 《中国工作犬业》2007,(2):29-29

某地一头犬长期消瘦,在一次出现场回来后突然发病,经临床和血液检查分析,确诊为附红细胞体感染,经及时治疗,该犬已痊愈。现将诊疗经过报道如下:  相似文献   

Nitrogen balance in barrows fed low-protein diets supplemented with essential amino acids   总被引：3，自引：0，他引：3  

D. Deng  R.L. Huang  T.J. Li  G.Y. Wu  M.Y. Xie  Z.R. Tang  P. Kang  Y.M. Zhang  M.Z. Fan  X.F. Kong  Z. Ruan  H. Xiong  Z.Y. Deng  Y.-L. Yin   《Livestock Science》2007,109(1-3):220-223

This study was conducted to determine the effect of addition of essential amino acids (EAA) to low-protein diets on N balance in barrows. Thirty barrows (Duroc × Yorkshire) with an initial BW of 36 kg were fed 5 corn- and soybean meal-based diets containing 13.6 to 18.2% crude protein (CP) for 10 d in a randomized complete block design. The 18.2% CP diet was formulated without addition of EAA (Lys, Met or Thr). The other diets contained 13.6 to 16.5% CP and were supplemented with EAA to provide 0.83% true digestible lysine equal to that in the 18.2% CP diet. Fecal, urinary, and total N excretion as well as N retention (g/d) decreased with decreasing dietary CP level (P < 0.01). The apparent digestibility of N was almost the same for the 18.2 and 16.5 CP diets, but the value was 2% higher (P = 0.07) than that for the 13.6% CP diet. These findings indicate that, compared with the 18.2% CP diets, supplementation with three EAA to the lowest-protein diets (13.6 CP) is inadequate for maximum intestinal protein digestion, amino acid absorption, or tissue protein deposition in growing barrows.  相似文献   

First report of Papaya leaf curl China virus infecting Corchoropsis timentosa in China     

J. F. Huang  X. P. Zhou 《Plant pathology》2006,55(2):291-291

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Spatial and temporal patterns of Lolium rigidum–Avena sterilis mixed populations in a cereal field     

J M BLANCO-MORENO  L CHAMORRO  & F X SANS 《Weed Research》2006,46(3):207-218

Through a detailed case study of a two‐species (Lolium rigidum and Avena sterilis) weed community at contrasting scales, this paper examined factors that affect weed distribution across space and time in a commercial wheat field in north‐east Spain. A. sterilis showed relatively stable spatial distribution and spatial structure of its population over time at large scale, with well‐defined patches, although weed density rose quickly. L. rigidum showed poorly defined patches that were not stable across time. Interaction between species could explain to some degree the spatial distribution at large scale: a negative relationship was detected between the spatial structures of both weed populations. At fine scale, both species showed a clear interaction effect from primary dispersal (more important in A. sterilis) and secondary dispersal from combine harvesting (more important in L. rigidum).  相似文献   

Toxicity and biochemical action of amicarthiazol on citrus canker pathogen, Xanthomonas citri ex Hasse     

Qingchun Huang  Jihua Wu  Xiaotao Li  Manhui Liu  Yuping Kong 《Pesticide biochemistry and physiology》2006,84(3):188-195

Inhibitory effects of amicarthiazol, in vitro, against the growth of seven plant bacterial pathogens and its biochemical actions on Xanthomonas citri were investigated. The growth of Erwinia carotovora, Ralstonia solanacearum, and Pseudomonas syringe pv. syringae was not sensitive to amicarthiazol whilst Xanthomonads were all strongly inhibited in NB liquid medium; EC₅₀ of X. citri was 2.63 μg ml⁻¹ and the effectiveness of amicarthiazol depended on the timing of application. The inhibitions on the secretion of gross EPS and gross EPR as well as the activities of amylase, cellulase, and pectase of X. citri were increased with the concentrations of amicarthiazol, whereas the conductivities of the extracellular product suspensions and the protease activity were somewhat accelerated by the concentration less than 10 or 20 μg ml⁻¹ and inhibited only at higher concentration. EPS which was purified from X. citri showed obvious antagonistic effects on the inhibition of amicarthiazol, but not on the inhibition of Cu(OH)₂. The endogenous respirations and the activity of succinic dehydrogenase of X. citri were also inhibited and the inhibitions were increased with the toxic concentrations. Furthermore, the potential induced physiological adaptability of X. citri to amicarthiazol and the comparison with Cu(OH)₂ and carboxin were also discussed.  相似文献   

土壤容重对生姜生长及产量和品质的影响   总被引：1，自引：0，他引：1  

尚庆文  徐坤  孔祥波  王玉霞 《中国蔬菜》2006,1(11):18-20

以莱芜大姜为试材,研究了容重分别为1.49(CK)、1.36(T1)、1.20(T2)g·cm-3的3种土壤对生姜生长及产量、品质的影响。结果表明,容重较小的土壤,其孔隙度较大,最大持水量较高,有利于生姜植株的生长和产量的提高。生长末期T2的株高、茎粗、分枝数和叶片数分别比CK增加17.9%、14.0%、35.2%和36.1%,单株根、茎、叶、根茎鲜质量分别比CK提高43.3%、24.4%、28.1%和35.2%,收获时CK、T1、T2的产量分别为2534.1、3017.7和3326.8kg·(667m2)-1,处理间差异极显著。此外,生姜根茎干物质、淀粉及挥发油含量随土壤容重降低而降低,而蛋白质、游离氨基酸则相反,粗纤维、可溶性糖及VC含量则基本不受土壤容重的影响。  相似文献   

J05Introduction of the concept of the 'Qualified Person' in the context of on-farm production and application of medicated feed in Switzerland     

S. HERRLI  B. GASSNER  O. FLECHTNER  X. SIDLER  A. LUGINBÜHL  W. KOCH  & R. ZWAHLEN 《Journal of veterinary pharmacology and therapeutics》2006,29(S1):309-309

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