Preparation of activated carbon moldings from the mixture of waste newspaper and isolated lignins: mechanical strength of thin sheet and adsorption property   总被引：2，自引：0，他引：2  

Yasumitsu Uraki  Satoshi Kubo  Yoshihiro Sano 《Journal of Wood Science》2002,48(6):521-526

To expand the utilization of waste newspapers and lignin, activated carbon (AC) sheets, as an example of AC moldings, were prepared from those mixtures. The isolated lignins used were softwood and hardwood acetic acid lignins (SAL and HAL), softwood kraft lignin (KL), and wheat-straw lignin (WSL). The mixtures were molded into precursory sheets by thermal compression and then converted to AC sheets by carbonization and steam activation. The flexural strength of the precursory sheets was dramatically improved by additing the lignins compared to that of sheets without lignin. The strength of several sheets was more than 25 MPa. This suggested that lignins act as adhesives. SAL and HAL sheets with 40% newspaper were strengthened by the carbonization, whereas the strength of other lignin sheets was depressed. Finally, the AL-based AC sheets showed higher flexural strength (>6MPa) than others. Most of the AC sheets had adsorption ability comparable to that of commercially available AC powder and granules. The capacities were almost independent of paper content. Among the AC moldings tested, the AL-based AC sheets showed the fastest adsorption top-chlorophenol. Thus, viable AC moldings can be prepared from lignin-wastepaper mixtures, particularly SAL and HAL.  相似文献   

Effect of estradiol‐17β during in vitro growth culture on the growth,maturation, cumulus expansion and development of porcine oocytes from early antral follicles          下载免费PDF全文

Naoko Kubo  Ilse Silvia Cayo‐Colca  Takashi Miyano 《Animal Science Journal》2015,86(3):251-259

Growing porcine oocytes from early antral follicles can acquire meiotic and developmental competence under suitable culture conditions, but at lower rates compared to full‐grown oocytes. We postulated that estradiol‐17β (E₂) supported the acquisition of meiotic and developmental competence as well as cumulus‐expansion ability during growth culture. Growing oocytes from early antral follicles (1.2 to 1.5 mm in diameter) were grown in vitro for 5 days in a medium containing 0, 10^?7, 10^?6, 10^?5 or 10^?4 mol/L E₂; after in vitro maturation, 35, 58, 47, 74 and 49% of oocytes matured to metaphase II, 25, 79, 77, 90 and 97% acquired cumulus‐expansion ability, and 23, 54, 63, 89 and 64% were fully surrounded by cumulus cells, respectively. Following maturation, electro‐stimulation was applied to the oocytes grown with 10^?5 mol/L E₂. After 6 days of culture, in vitro‐grown oocytes developed to the blastocyst stage at a rate similar to that for full‐grown oocytes (31% and 40%, respectively). Therefore, we suggest that the use of E₂ during growth culture improves the meiotic and developmental competence of oocytes, cumulus‐expansion ability, and cumulus cell attachment to the oocytes.  相似文献   

Morphological changes in the cytoskeleton, nuclei, and vacuoles during cell death of short-lived ray tracheids in the conifer Pinus densiflora     

Satoshi Nakaba  Joto Yoshimoto  Takafumi Kubo  Ryo Funada 《Journal of Wood Science》2008,54(6):509-514

Morphological changes in the cytoskeleton, nuclei, and vacuoles were monitored during the cell death of short-lived ray tracheids in the conifer Pinus densiflora. After formation of the dentate thickenings that occurred at the final stage of formation of cell walls, organelles started to disappear in differentiating ray tracheids. First, the microtubules and vacuoles disappeared. Then actin filaments disappeared in the differentiating ray tracheids adjacent to ray tracheids that lacked nuclei, and, finally, the nuclei disappeared. These features indicate that cell death in ray tracheids might differ from the programmed cell death of tracheary elements that has been studied in vitro in the Zinnia culture system. This study was presented at the 57th Annual Meeting of the Japan Wood Research Society, August 8–10, 2007, Hiroshima, Japan  相似文献   

Maturation and plant recovery from embryogenic cells of Japanese larch: Effect of abscisic acid in relation to their morphology     

Shinjiro Ogita  Hamako Sasamoto  Takafumi Kubo 《Journal of Forest Research》1999,4(3):241-244

Two embryogenic cell lines characterized by different morphology and color, white and red, were obtained from an immature zygotic embryo of Japanese larch (Larix leptolepis Gord.). Mature somatic embryos with cotyledons and regenerated plants were obtained from both cell lines. However optimal concentration of abscisic acid (ABA) for maturation varied depending on morphology of ECs. From the white ECs which intermingled with somatic embryos of very early stage, mature somatic embryos were induced on maturation medium containing 50 μM of ABA. On the other hand, mature somatic embryos with cotyledons were observed from the red ECs which consisted of somatic embryos of more developed stage on hormone-free medium or 0.1 μM ABA containing-medium.  相似文献   

Ring dike system to harness floodwater from the Mekong River for paddy rice cultivation in the Tonle Sap Lake floodplain in Cambodia     

P. Someth  N. Kubo  H. Tanji 《Agricultural Water Management》2009,96(1):100-110

Located in the floodplain of the Mekong River and the Tonle Sap River, Batheay irrigation system and its reservoir directly receive floodwater from the Mekong. The Batheay reservoir formed by a ring dike functions as both a reservoir and a paddy field. In the wet season, the ring dike prevents floodwater from entering the reservoir and rainy season rice is grown inside the dike. After harvesting, the gates on the ring dike are opened to receive floodwater. The water is stored inside the dike for cultivating dry season rice outside the dike. In this paper, the irrigation system is studied as a model site for future development of the floodplain of the Tonle Sap Lake of Cambodia. Specifically, this paper is concerned with the study of water balance and analysis of the hydrologic components of the Batheay irrigation system, and the effectiveness of the ring dike system. The study found that floodwater of the Mekong River contributed about 74% to the total inflow to the Batheay reservoir. Contributions to the total water supply of reservoir water, floodwater remaining in the fields, and precipitation were 73, 12, and 15%, respectively. The efficiency of the system was found to be 92%. The dike system is expected to be a paradigm for the floodplain of the Tonle Sap Lake.  相似文献   

Preparation of Anti-Rabies Virus N Protein IgYs by DNA Immunization of Hens Using Different Types of Adjuvants     

Nanase Kubo  Mari Nishii  Satoshi Inoue  Akira Noguchi  Hajime Hatta 《The Journal of Poultry Science》2022,59(2):191

DNA immunization has been used to study vaccination methods and for production of specific antibodies. The present study aimed to apply DNA immunization to prepare specific IgYs, which react against rabies virus N protein (RV-N) and can be used to research and diagnose rabies virus. The DNA sequence of RV-N was ligated into a pcDNA 3.1 plasmid for constructing pcDNA-N. Eight hens were divided into four groups. Group 1 comprised the control group (non-immunized). In Groups 2, 3, and 4, hens were injected intramuscularly with pcDNA-N (400 µg/hen). Eight injections were administered every other week. From the 4th week, an adjuvant was injected in addition to pcDNA-N. Freund''s complete adjuvant (FCA) and λ-carrageenan were administered to Groups 3 and 4, respectively. Eggs were collected daily, and the specific antibody activities of egg yolks were measured by ELISA. IgYs were purified from pooled egg yolks at 16–19 weeks post-administration in each group. The detection sensitivities of the RV-N were compared using purified IgY as the primary antibody for ELISA, dot blotting, and western blotting. Egg yolks from one of the two hens in Group 2 (pcDNA-N alone) and all hens in Groups 3 (pcDNA-N + FCA) and 4 (pcDNA-N + λCarra) had increased ELISA values. The combined use of λ-carrageen in DNA immunization resulted in an adjuvant effect comparable to that of FCA. Each purified specific IgY detected RV-N in the ELISA, western blotting, and dot blotting; however, the detection sensitivity differed. Higher detection sensitivity of the +λCarra IgY was observed by ELISA, whereas there was higher detection sensitivity of +FCA IgY in western blotting and dot blotting. In summary, anti-rabies virus N protein IgY was prepared through DNA immunization of hens using FCA or λ-carrageenan as adjuvants and can be used as a primary antibody to detect rabies viruses.  相似文献   

Effects of thymol on B16-F10 melanoma cells     

Satooka H  Kubo I 《Journal of agricultural and food chemistry》2012,60(10):2746-2752

Aromatic monoterpene, thymol, shows several beneficial activities, such as an antioxidative effect. However, the mechanism of its toxicity remains to be fully defined. In preliminary studies, thymol was characterized as a melanin formation inhibitor in an enzymatic system; however, thymol showed moderate cytotoxicity but not an antimelanogenic effect on B16-F10 melanoma cells. Thymol exhibited cytotoxicity, with an IC(50) value of 400 μM (60.09 μg/mL). This moderate toxic effect was suppressed with the addition of vitamin C and vitamin D, and 20 and 40% of cell viability was increased, respectively. Subsequently, the treatment of L-cysteine on thymol-treated melanoma cells reversed the toxic effect of thymol. Moreover, a significant oxidative stress condition was observed when B16 melanoma cells were cultured with thymol. In conclusion, the antioxidant actions of thymol generate a stable phenoxy radical intermediate, which generates reactive oxygen species and quinone oxide derivatives. Thus, it is proposed that the primary mechanism of thymol toxicity at high doses is due to the formation of antioxidant-related radicals.  相似文献   

Construction of a chromosome-assigned,sequence-tagged linkage map for the radish,Raphanus sativus L. and QTL analysis of morphological traits     

Tomoko Hashida  Ryoichi Nakatsuji  Holger Budahn  Otto Schrader  Herbert Peterka  Tatsuhito Fujimura  Nakao Kubo  Masashi Hirai 《Breeding Science》2013,63(2):218-226

The radish displays great morphological variation but the genetic factors underlying this variability are mostly unknown. To identify quantitative trait loci (QTLs) controlling radish morphological traits, we cultivated 94 F₄ and F₅ recombinant inbred lines derived from a cross between the rat-tail radish and the Japanese radish cultivar ‘Harufuku’ inbred lines. Eight morphological traits (ovule and seed numbers per silique, plant shape, pubescence and root formation) were measured for investigation. We constructed a map composed of 322 markers with a total length of 673.6 cM. The linkage groups were assigned to the radish chromosomes using disomic rape-radish chromosome-addition lines. On the map, eight and 10 QTLs were identified in 2008 and 2009, respectively. The chromosome-linkage group correspondence, the sequence-specific markers and the QTLs detected here will provide useful information for further genetic studies and for selection during radish breeding programs.  相似文献   

Towards the partial resumption of agriculture with buckwheat cultivation in fields physically decontaminated of radioactive cesium after the nuclear power plant accident in 2011: a case study in Yamakiya District,Fukushima     

Katashi Kubo  Hiroyuki Kobayashi  Ryusuke Fujimoto  Takeshi Ota  Takuro Shinano 《Plant Production Science》2013,16(2):159-167

ABSTRACT

Yamakiya District in the town of Kawamata, Fukushima Prefecture, was evacuated after the nuclear accident at the Tokyo Electric Power Company’s Fukushima Dai-ichi nuclear power plant in 2011. Since then, nuclear decontamination procedures have been applied to the surrounding environment, including agricultural fields. The decontamination procedure for agricultural fields consists of the removal of radiation-contaminated surface soil, followed by soil dressing and plowing. However, radioactive cesium (RCs) remains in the soil even after decontamination. In this study, we investigated the effect of applying nitrogen, potassium fertilizers, and cattle manure compost on buckwheat growth and the concentration of RCs in the grain of buckwheat cultivated in a decontaminated field from 2014 to 2016. Applications of potassium fertilizer and cattle manure compost increased the soil exchangeable potassium content and decreased the RCs concentration in the grain of buckwheat cultivated in the decontaminated field. Before the cultivation of the first buckwheat crop, the RCs concentration in soil varied widely and there were ‘hot spots’ with high RCs concentrations because of insufficient mixing of the original and the dressed soils. Therefore, soil had to be adequately mixed to avoid producing grain with a high RCs concentration. Buckwheat grew better when supplied with more nitrogen fertilizer than the conventional amount at the first cultivation, indicating that the dressed soil had low fertility. We also monitored buckwheat cultivation by local farmers in decontaminated fields from 2015 to 2017. By using potassium fertilizer, the farmers produced buckwheat grain with low RCs concentrations from 2015 to 2017.  相似文献   

Green and black tea suppress hyperglycemia and insulin resistance by retaining the expression of glucose transporter 4 in muscle of high-fat diet-fed C57BL/6J mice     

Nishiumi S  Bessyo H  Kubo M  Aoki Y  Tanaka A  Yoshida K  Ashida H 《Journal of agricultural and food chemistry》2010,58(24):12916-12923

To investigate the preventive effects of tea on hyperglycemia and insulin resistance, male C57BL/6J mice were given a high-fat diet containing 29% lard and also green or black tea ad libitum for 14 weeks. Both teas suppressed body weight gain and deposition of white adipose tissue caused by the diet. In addition, they improved hyperglycemia and glucose intolerance by stimulating glucose uptake activity accompanied by the translocation of glucose transporter (GLUT) 4 to the plasma membrane in muscle. Long-term consumption of the high-fat diet reduced levels of insulin receptor β-subunit, GLUT4 and AMP-activated protein kinase α in muscle, and green and black tea suppressed these decreases. The results strongly suggest that green and black tea suppress high-fat diet-evoked hyperglycemia and insulin resistance by retaining the level of GLUT4 and increasing the level of GLUT4 on the plasma membrane in muscle.  相似文献