Role of amyloid type cross beta-structure in the formation of soluble aggregate and gel in heat-induced ovalbumin     

Azakami H  Mukai A  Kato A 《Journal of agricultural and food chemistry》2005,53(4):1254-1257

The heat-induced denaturation curve of ovalbumin followed by the ellipticity at 222 nm in circular dichroism spectra was consistent with that monitored by fluorescence with thioflavin T, which is an indication of amyloid fibril formation, while other proteins such as lysozyme and ovotransferrin did not fluoresce with thioflavin T during heat denaturation. The amount of soluble aggregate formed during heat denaturation was proportional to the increase in fluorescence with thioflavin T. The binding of soluble aggregates with thioflavin T was greatly suppressed in heat-denatured ovalbumin in the presence of thioflavin T. The similar inhibition effect of thioflavin T on the gel formation of heat-induced ovalbumin was observed. These results suggest that the amyloidogenic intermolecular beta-structure is involved in the formation of soluble aggregate and gel of heat-induced ovalbumin.  相似文献   

Determination of polysorbates in foods by colorimetry with confirmation by infrared spectrophotometry, thin-layer chromatography, and gas chromatography   总被引：1，自引：0，他引：1  

H Kato  Y Nagai  K Yamamoto  Y Sakabe 《Journal of the Association of Official Analytical Chemists》1989,72(1):27-29

A method is presented for the detection of polysorbates (PSs) in 8 kinds of processed foods by colorimetric and thin-layer chromatographic (TLC) techniques. The PSs are extracted from processed foods with a mixture of methylene chloride and ethanol by using an Extrelut column. The extract is further purified by using a silica gel column. The PS extract is complexed with cobalt-thiocyanate (Cothiocyanate) reagent and is determined spectrophotometrically at 620 nm. The recoveries and coefficients of variation for 8 kinds of processed foods fortified with 0.1% PS 80 were 67.9-94.6% and 4.0-11.3%, respectively. The detection limit of TLC corresponded to 50 mg PS 80/kg. PS identity was confirmed by infrared spectrophotometry of PS extract, and gas chromatography of fatty acids and thin layer chromatography of POE-sorbitan residues after saponification.  相似文献   

Antioxidative properties of phenolic antioxidants isolated from corn steep liquor     

Niwa T  Doi U  Kato Y  Osawa T 《Journal of agricultural and food chemistry》2001,49(1):177-182

With the immersion of corn into dilute sulfur oxide during starch-manufacturing processes, corn steep liquor (CSL) remains as leftover material. CSL is often used for fermentation, but its components are not fully understood. To determine the properties of CSL, 12 p-coumaric acid-related compounds were isolated from an ethyl acetate extract of CSL with the guidance of antioxidative activity on the rabbit erythrocyte membrane ghost system. The activity of these compounds was compared against oxidative damages, and it was elucidated that the activity of p-coumaric acid derivatives was mainly affected by their functional groups at the 3-position and less by the conjugated side chain. Moreover, p-coumaric acid derivatives exhibited inhibitory activity stronger than that of tocopherols and ascorbic acid on peroxynitrite-mediated lipoprotein nitration. These findings that p-coumaric acid derivatives, which might play a beneficial role against oxidative damage, exist in CSL suggest this byproduct might be a useful resource of phenolic antioxidants.  相似文献   

Improvement of rice (Oryza sativa L.) growth in upland conditions with deep tillage and mulch   总被引：1，自引：0，他引：1  

Yoichiro Kato  Akihiko Kamoshita  Jun Abe  Junko Yamagishi 《Soil & Tillage Research》2007,92(1-2):30-44

The productivity of upland rice in Japan as well as in the world is low and unstable owing to scarce and unpredictable rainfall. The objective of this study was to examine whether agronomic methods could enhance grain yield of upland rice. Four field experiments were conducted from 2001 to 2003 in Nishitokyo, Japan, under upland conditions with different water supplies, in order to quantify the effects of deep tillage combined with deep placement of manure (50-cm depth), straw mulch (6 t ha⁻¹), or their combinations on the growth and grain yield of rice. Mulch kept surface soil moisture higher than without mulch even at reproductive stage, and it increased yield to the greatest extent under the most favourable conditions with much rainfall before heading (i.e., 2003). Deep tillage with deep placement of manure induced deep root proliferation and higher nitrogen uptake, increasing biomass production, and panicle number, and consequently grain yield was enhanced under the two lowest yielding environments with less rainfall before heading. Rice plants with deep tillage with deep manure application without mulch tended to have lower leaf water potential and higher diffusion resistance during drought, and negative effects on grain filling and harvest index in some experiments compared with the control. When deep tillage with deep placement of manure was combined with mulching in two experiments in 2002 and 2003, grain yield always enhanced compared with the control (P < 0.10, 6.0 t ha⁻¹ versus 5.4 t ha⁻¹ on average), suggesting their synergetic mechanisms for yield increase and stabilization. The results showed that deep tillage or mulching can improve grain yield of rice under drought-prone rainfed upland conditions in a temperate climate on an Andosol, and their combination had more consistent and greater positive effects.  相似文献   

Effects of nitric oxide donor and nitric oxide synthase inhibitor on ruminal contractions in conscious sheep     

T. Onaga  H. Okada  S. Hagiwara  C. Nagashima  H. Inoue  W. Korczynski  S. Kato 《Research in veterinary science》2001,71(3):189-195

The present study was planned to evaluate a role of nitric oxide (NO) in the regulation of regular ruminal contractions in conscious sheep. Intravenous infusion of S-nitroso-acetyl-DL-penicillamine (SNAP) at doses of 3-30 nmol kg(-1) min(-1)for 30 minutes inhibited both the amplitude and frequency of ruminal contractions in a dose-dependent manner. However, intravenous infusion of Nomega-nitro-L-arginine-methyl ester (L-NAME) at doses of 0.3-3.0 micromol kg(-1) min(-1)did not alter the basal tone of intraruminal pressure and the amplitude of ruminal contractions. The frequency of contractions was slightly inhibited by L-NAME infusion at 1.0 micromol kg(-1)min(-1). The effects of L-NAME were abolished by simultaneous infusion of L -arginine at 30 micromol kg(-1) min(-1). These results suggest that exogenous NO can diminish the ruminal contractions, while endogenous NO is not involved in the regulatory mechanism of basal tone and regular phasic contractions of the rumen in healthy sheep.  相似文献   

Effect of canine adenovirus on JINET and Vero cells.     

T Murakami  H Kato  K Takada 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1977,39(3):283-292

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Difference in glycosphingolipid compositions of avian Marek's disease lymphoma-derived cell lines and lymphoid leukosis lymphoma-derived cell lines     

Y Fujii  K Ikuta  S Kato  T Mikami  M Naiki 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(2):471-479

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Bacteriophage typing of Staphylococcus aureus isolated from dogs in Japan.     

A Shimizu  E Kato 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1979,41(4):405-408

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Swine fever. Immunisation of piglets   总被引：4，自引：0，他引：4  

P Precausta  F Kato  A Brun 《Comparative immunology, microbiology and infectious diseases》1983,6(4):281-289

Vaccination against Swine Fever using the CL Chinese strain can be done in 7-day-old piglets if they are born of non-immune sows. The simultaneous weaning and vaccination emphasises the safety of this strain. The excellent immunity observed confirms the immunocompetence of 7-day-old piglets. In piglets born of immune sows and also weaned at 7 days, passive protection can extend beyond the age of 2 months if the sow is vaccinated several months prior to gestation. The immune level of the piglets would seem to depend on the interval between vaccination of the sow and farrowing and can be attributed to the quality of the antibodies transmitted by the colostrum. Piglets born of sows vaccinated 10 months prior to farrowing can be vaccinated as early as 5 weeks; the protection percentage observed at the age of about 6 months is over 80%. A booster injection at this age then confers immunity to future breeders throughout their economic life, i.e. 4 years in the reported experiment.  相似文献   

ANTERIOR CRUCIATE LIGAMENT RECONSTRUCTION USING A COMPOSITE COLLAGENOUS PROSTHESIS: A BIOMECHANICAL AND HISTOLOGIC STUDY IN RABBITS     

Dunn MG  Tria AJ  Kato YP  Bechler JR  Ochner RS  Zawadsky JP  Silver FH 《Veterinary surgery : VS》1993,22(1):56-56

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