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31.
Performance characteristics of the turbidimetric ACL‐TOP CTS 300 coagulation analyzer in dogs and cats
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Sanchez-Ballesta MT Zacarias L Granell A Lafuente MT 《Journal of agricultural and food chemistry》2000,48(7):2726-2731
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PC Rodriguez LB Valdez T Zaobornyj A Boveris MT Beconi 《Reproduction in domestic animals》2011,46(1):74-81
The aim of this work was to quantify NO, O2? and ONOO? production during heparin‐induced capacitation of cryopreserved bovine spermatozoa. A time dependent hyperbolic increase was observed for heparin‐dependent capacitation, O2 uptake, and NO production. Conversely, O2? production was increased during the first 15 min of incubation, showing a decrease from this time until 45 min. At 15 min of heparin incubation, a threefold increase in O2 consumption (5.9 ± 0.6 nmol/min × 107 cells), an enhancement in NO release (1.1 ± 0.2 nmol/min × 107 cells), and a five‐fold increase in O2? production (1.3 ± 0.07 nmol/min × 107 cells), were observed. Peroxynitrite production rate was estimated taking into account NO and O2? generation and the second‐order rate constant of the reaction between these species. To conclude, heparin‐induced capacitation of cryopreserved bovine spermatozoa activates (i) mitochondrial O2 uptake by high ADP levels due to increased energy requirements, (ii) NO production by a constitutive NOS and (iii) O2? production by a membrane‐bound NAD(P)H oxidase. The products of both enzymes are released to the extracellular space and could be involved in the process of sperm capacitation. 相似文献
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Hydrographic phenomena influencing early life stages of the Sicilian Channel anchovy 总被引:7,自引:0,他引:7
J. García Lafuente A. García S. Mazzola L. Quintanilla J. Delgado A. Cuttita & B. Patti 《Fisheries Oceanography》2002,11(1):31-44
The information collected from a European Union funded project on the ‘Distribution Biology and Biomass Estimates of the Sicilian Channel Anchovy (Engraulis encrasicolus)’ was used to analyse the linkage between the general circulation pattern of the Atlantic Ionian Stream (AIS) and the reproductive strategy of the Sicilian Channel anchovy. The main spawning ground is located in the NW region of the southern Sicilian coast. This region is a stable area of low current produced by the impingement towards the coast of the AIS and its bifurcation into two branches. The main branch heads towards the SE end of the Sicilian coast (Cape Passero) acting as a transport mechanism for the anchovy eggs and larvae. Along the AIS trajectory, there is a density front to the left of the current, facing downstream. This front is a consequence of the shoreward sloping of isopycnals that maintains the geostrophic flow, facilitating the mixing of deeper waters with surface layers and fertilization of coastal waters. The front enhances primary production assuring food availability for anchovy larvae during their advection by the AIS. The highest concentrations of larval anchovy were found off the SE Sicilian coast, in the area off Cape Passero. The greater average sizes of larvae found in this region, and their estimated age, support the evidence of advection by the AIS. The hydrographic features observed in this area, such as the existence of a well‐defined cyclonic vortex, implies the existence of upwelling in its centre, providing a suitable environment for sustained enhanced rates of primary production and allowing the larval population to maintain their relative position. This retention area is conceived as favourable for providing the necessary feeding conditions. The data acquired from a survey carried out to evaluate the anchovy recruitment strength confirm that larvae reach the juvenile stage in the south‐eastern coast of Sicily, since most of the young‐of‐the‐year anchovy were located in the Cape Passero region. 相似文献
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MK Gagan LK Ayliffe D Hopley JA Cali GE Mortimer J Chappell MT McCulloch MJ Head 《Science (New York, N.Y.)》1998,279(5353):1014-1018
Skeletal Sr/Ca and 18O/16O ratios in corals from the Great Barrier Reef, Australia, indicate that the tropical ocean surface approximately 5350 years ago was 1 degrees C warmer and enriched in 18O by 0.5 per mil relative to modern seawater. The results suggest that the temperature increase enhanced the evaporative enrichment of 18O in seawater. Transport of part of the additional atmospheric water vapor to extratropical latitudes may have sustained the 18O/16O anomaly. The reduced glacial-Holocene shift in seawater 18O/16O ratio produced by the mid-Holocene 18O enrichment may help to reconcile the different temperature histories for the last deglaciation given by coral Sr/Ca thermometry and foraminiferal oxygen-isotope records. 相似文献
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Carbohydrate metabolism as related to high-temperature conditioning and peel disorders occurring during storage of citrus fruit 总被引:1,自引:0,他引:1
The aim of this research was to understand the involvement of the carbohydrate metabolism in physiological disorders occurring during the postharvest storage of citrus fruit. These disorders, manifested in the rind, depreciate fruit quality and often originate important losses. There has been increasing interest in the use of nonharmful treatments, such as high-temperature conditioning, to avoid citrus peel damage during fruit storage at low temperature in chilling-sensitive cultivars, but their influence in postharvest disorders occurring at nonchilling temperatures and the mechanisms related to them are poorly understood. The data obtained showed that heat conditioning (3 days/37 degrees C) increases the chilling tolerance of cv. Navelate fruit and favored sucrose, but not hexoses, accumulation and its maintenance after the fruit was transferred to low temperature. This effect was related to heat-induced increase in the activities of the sucrose-synthesizing enzymes sucrose phosphate synthase (SPS) and sucrose synthase (SS). Furthermore, sucrose levels and the activities of both enzymes were higher in cv. Pinalate oranges, a chilling-tolerant spontaneous abscisic acid deficient mutant of Navelate. In contrast, carbohydrates appeared not to be involved in the susceptibility of oranges to rind staining, a physiological disorder different from chilling injury, which mainly occurred at a nonchilling temperature (12 degrees C) and was not reduced by heat conditioning. The effect of low temperature in SS and SPS activities was less than that of high temperature, which might be related to the lower changes occurring in sucrose during fruit storage at 2 degrees C. 相似文献