Comparison of faecal strongylate egg counts of goats and sheep on the same pasture     

Pomroy WE  Lambert MG  Betteridge K 《New Zealand veterinary journal》1986,34(3):36-37

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A 13CO2 Enrichment Experiment to Study the Synthesis Pathways of Polyunsaturated Fatty Acids of the Haptophyte Tisochrysis lutea     

Marine Remize  Frdric Planchon  Matthieu Garnier  Ai Ning Loh  Fabienne Le Grand  Antoine Bideau  Christophe Lambert  Rudolph Corvaisier  Aswani Volety  Philippe Soudant 《Marine drugs》2022,20(1)

The production of polyunsaturated fatty acids (PUFA) in Tisochrysis lutea was studied using the gradual incorporation of a ¹³C-enriched isotopic marker, ¹³CO₂, for 24 h during the exponential growth of the algae. The ¹³C enrichment of eleven fatty acids was followed to understand the synthetic pathways the most likely to form the essential polyunsaturated fatty acids 20:5n-3 (EPA) and 22:6n-3 (DHA) in T. lutea. The fatty acids 16:0, 18:1n-9 + 18:3n-3, 18:2n-6, and 22:5n-6 were the most enriched in ¹³C. On the contrary, 18:4n-3 and 18:5n-3 were the least enriched in ¹³C after long chain polyunsaturated fatty acids such as 20:5n-3 or 22:5n-3. The algae appeared to use different routes in parallel to form its polyunsaturated fatty acids. The use of the PKS pathway was hypothesized for polyunsaturated fatty acids with n-6 configuration (such as 22:5n-6) but might also exist for n-3 PUFA (especially 20:5n-3). With regard to the conventional n-3 PUFA pathway, Δ6 desaturation of 18:3n-3 appeared to be the most limiting step for T. lutea, “stopping” at the synthesis of 18:4n-3 and 18:5n-3. These two fatty acids were hypothesized to not undergo any further reaction of elongation and desaturation after being formed and were therefore considered “end-products”. To circumvent this limiting synthetic route, Tisochrysis lutea seemed to have developed an alternative route via Δ8 desaturation to produce longer chain fatty acids such as 20:5n-3 and 22:5n-3. 22:6n-3 presented a lower enrichment and appeared to be produced by a combination of different pathways: the conventional n-3 PUFA pathway by desaturation of 22:5n-3, the alternative route of ω-3 desaturase using 22:5n-6 as precursor, and possibly the PKS pathway. In this study, PKS synthesis looked particularly effective for producing long chain polyunsaturated fatty acids. The rate of enrichment of these compounds hypothetically synthesized by PKS is remarkably fast, making undetectable the ¹³C incorporation into their precursors. Finally, we identified a protein cluster gathering PKS sequences of proteins that are hypothesized allowing n-3 PUFA synthesis.  相似文献   

Testing the ability of unmanned aerial systems and machine learning to map weeds at subfield scales: a test with the weed Alopecurus myosuroides (Huds)     

James PT Lambert  Dylan Z Childs  Rob P Freckleton 《Pest management science》2019,75(8):2283-2294

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From shallow to deep waters: habitats used by larval lampreys (genus Petromyzon and Lampetra) over a western European basin     

Catherine Taverny  Isabelle Ortusi  Charles Roqueplo  Mario Lepage  Patrick Lambert 《Ecology of Freshwater Fish》2012,21(1):87-99

Abstract –  Habitat requirements of lamprey ammocoetes (Petromyzon marinus and Lampetra genus) were investigated, for the first time, from shallow to deep waters, at different spatial scales across the Gironde‐Dordogne continuum, thanks to a water suction dredge. Fish‐habitat relationships were assessed through two complementary statistical analyses: habitat‐use curves and habitat suitability models using the Boosted Regression Trees (BRT) technique. Analyses were performed on a small‐size data set that was characterised by the low prevalence of lamprey. The sea lamprey larvae occurred in deeper areas than their Lampetra genus counterparts. ‘Pools’ of 2 m’ depth and more were optimal habitats for the former species. Among the environmental variables retained to model lamprey occurrences, the mesohabitat (a categorical variable) was demonstrated to be highly influential, in terms of fine grain‐size substratum and vegetation cover. These preliminary results suggest that monitoring using the water suction dredge method may contribute to sea lamprey conservation.  相似文献   

Effects of supplemental alfalfa hay on the digestion of soybean hull-based diets by cattle     

Trater AM  Titgemeyer EC  Löest CA  Lambert BD 《Journal of animal science》2001,79(5):1346-1351

We evaluated the optimal level of alfalfa inclusion in soybean hull-based diets. In Exp. 1, 20 Holstein steers (319 kg of BW) were used in a complete block design. Treatments included a soybean hull mix (95.7% soybean hulls, 3% molasses, 0.5% urea, 0.8% mineral mix; DM basis) fed alone (100:0) or with 10.4, 20.7, or 30.9% (DM basis) coarsely chopped alfalfa hay (90:10, 80:20, and 70:30, respectively) or alfalfa alone (0:100). Diets were fed once daily at 1.75% (DM basis) of BW. In some cases, orts were present, which caused DM, OM, and NDF intakes to decrease (linear, P < 0.05) as alfalfa was added to the diets. Digestibilities of DM, OM, and NDF decreased linearly (P < 0.05) as alfalfa was added to the diets, but quadratic responses (P < 0.05) indicated that positive associative effects occurred between soybean hulls and alfalfa. Liquid dilution rates increased (linear, P < 0.05) with alfalfa additions to the diets and also demonstrated positive associative effects between soybean hulls and alfalfa (quadratic, P < 0.05). Solid passage rates were similar for 100:0 and 0:100 but were increased (quadratically and cubically, P < 0.05) when combinations of soybean hulls and alfalfa were fed. In Exp. 2, in vitro NDF digestibilities were measured for soybean hulls, alfalfa, and a blend of 85% soybean hulls and 15% alfalfa, each with no N source or supplemented with casein or urea to ascertain the effects of protein from alfalfa on digestibility. Disappearances were increased (P < 0.05) by addition of urea or casein, but no interactions between substrate and N supplement were observed. Addition of 30% alfalfa to diets consisting primarily of soybean hulls led to positive associative effects on diet digestibility, but alfalfa additions led to increased liquid and solid passage rates, suggesting that the benefit was not a result of slower passage of soybean hulls from the rumen.  相似文献   

Epithelial cell dynamics in transmissible gastroenteritis of neonatal pigs     

D C Thake  H W Moon  G Lambert 《Veterinary pathology》1973,10(4):330-341

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SOWING GRASS AND CLOVER SEEDS IN THE AUTUMN     

A. G. Davis  J. P. Lambert 《Grass and Forage Science》1947,2(2):63-71

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Correlation between Chemical Structure and Biological Activity of Host-Selective Plant Pathogen Mycotoxins     

WEI Shuhua LIANG Jun YE Jianren ZHANG Xingyao** . School of Resources  Environment of Nanjing Forestry University  Nanjing  P.R. China . Institute of Forest Ecology  Environmental  Protection  Chinese Academy of Forestry  Beijing  P.R. China 《中国林业科技(英文版)》2007,6(1):62-68

Toxin, one of the most important factors of plant fungal disease, has attracted much attention of many academicians who have been studying pathogen mycotoxin in deep research. The paper summarized chemical structures of some host-selective plant pathogen mycotoxins discovered in recent years and the correlation between biological activity and chemical structure of toxin.  相似文献   

Serological survey for antibodies against bovine viral diarrhoea virus and Neospora caninum in a population of South Australian alpacas (Vicugna pacos)          下载免费PDF全文

PD Cockcroft  LE Dornin  RC Lambert  J Perry  MP Reichel 《Australian veterinary journal》2015,93(12):476-478

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Role of phospholipase A2 in the induction of drip loss in porcine muscle     

Poulsen KA  Young JF  Theil P  Kolko M  Oksbjerg N  Lambert IH 《Journal of agricultural and food chemistry》2007,55(5):1970-1976

The role of phospholipase A2 in the induction of drip loss from pig muscle has been investigated. In samples from porcine M. longissimus dorsi, total PLA2 activity as well as mRNA and protein levels of the group VIA iPLA2 (iPLA2-VIA) increased during the initial 4 h post-mortem period. Morphological studies of porcine muscle showed that at 4 h post-mortem, gaps had formed between muscle fibers and that the sarcolemma membrane borders appeared blurred. At the same time iPLA2-VIA protein levels were increased inside muscle fibers and at the sarcolemma. iPLA2-VIA mRNA abundance in samples from different breeds of pigs with variations in drip loss revealed no clear correlation between drip loss level and iPLA2-VIA expression. Together, these data indicate that during the post-mortem period, iPLA2-VIA expression and activity is increased at the muscle fiber membranes. PLA2 activity may affect membrane permeability and consequently the progression of drip formation in porcine muscle.  相似文献