Evaluation of Doppler ultrasonography for the measurement of blood flow in young loggerhead sea turtles (Caretta caretta)     

Valente AL  Parga ML  Espada Y  Lavin S  Alegre F  Marco I  Cuenca R 《Veterinary journal (London, England : 1997)》2008,176(3):385-392

The aim of this study was to identify ultrasound accessible blood vessels in the loggerhead sea turtle (Caretta caretta) and describe their Doppler waveform patterns, peak systolic velocity, mean velocity, systolic/diastolic ratio as well as pulsatility and resistive indices. B-mode, colour and pulsed-wave Doppler examinations were performed on 10 turtles. Flow measurements were recorded for the left and right aorta, epigastric and internal iliac arteries, and right hepatic vein. Additionally, the large blood vessels of three dead turtles were injected with latex and dissected for anatomical support. A parabolic flow velocity profile was observed in all arteries. The waveforms of the right and left aortic arteries showed an unusual pattern when compared with mammals. The hepatic vein flow velocity waveform of the loggerhead sea turtle was found to be similar to that in the dog, although the flow velocity in the C-wave was higher than that in the A-wave. The low resistance flow pattern observed suggests that the loggerhead sea turtle's organs require a continuous blood supply.  相似文献   

Blood group system in a captive population of European wildcats (Felis silvestris)     

Silvestre-Ferreira AC  Marco I  Daussa B  Piñol C  Lavin S  Pastor J 《The Veterinary record》2006,159(17):567-568

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Effects of diazepam, ketamine, and their combination on intraocular pressure in normal dogs     

EH Hofmeister  CB Mosunic  BT Torres  AG Ralph  PA Moore  MR Read 《Veterinary anaesthesia and analgesia》2005,32(4):11-12

Ketamine has been implicated as causing increases in intraocular pressure. The purpose of this study is to document the effects of ketamine, diazepam, and their combination on intraocular pressure (IOP) in normal, unpremedicated dogs. Random-source dogs were assigned to one of five groups of 10 dogs each: ketamine 5 mg kg^–1 (KET5), ketamine 10 mg kg^–1 (KET10), diazepam 0.5 mg kg^–1 (VAL), ketamine 10 mg kg^–1 with diazepam 0.5 mg kg^–1 (KETVAL), saline 0.1 mL kg^–1 (SAL), all given intravenously. A baseline IOP was measured before injection, immediately after injection, and at 5, 10, 15, and 20 minutes following injection. IOP was increased over baseline immediately after injection in the KET5, KET10, and KETVAL groups; at 5, 10, and 15 minutes in the KET5 group; and at 20 minutes in the KETVAL group. The mean IOP change compared to SAL increased immediately after injection and at 5 minutes in the KET5, KET10, and KETVAL groups; at 10 and 15 minutes in the KET5 group, and at 20 minutes in the KETVAL group. The mean IOP increased up to 5.7, 3.2, and 3.1 mm Hg over mean baseline in the KET5, KET10, and KETVAL groups, respectively. All dogs in the KET5 group and the majority in the KETVAL and KET10 groups had an increase in their IOP over baseline. Ketamine caused a clinically and statistically significant elevation in IOP over baseline and compared to SAL. The concurrent addition of diazepam did not blunt this increase. Ketamine should be avoided in dogs with corneal trauma, glaucoma, or in those undergoing intraocular surgery.  相似文献   

Solid phase microextraction application in gas chromatography/olfactometry dilution analysis   总被引：3，自引：0，他引：3  

Deibler KD  Acree TE  Lavin EH 《Journal of agricultural and food chemistry》1999,47(4):1616-1618

Gas chromatography/olfactometry (GC/O) based on dilution analysis (e. g., CharmAnalysis or aroma extraction dilution analysis) gives an indication of what compounds are most important (most potent) to the aroma of foods. The application of solid phase microextraction to the preparation of samples for GC/O dilution analysis was shown to be feasible by varying the fiber thickness and length to achieve various absorbant volumes.  相似文献   

The nucleotide sequence of the genes, fanE and fanF of Escherichia coli K99 fimbriae.     

E Ono  M F Lavin  M Naiki 《The Japanese journal of veterinary research》1991,39(1):1-10

K99 fimbriae of enterotoxigenic Escherichia coli consist of eight different subunits. A major subunit called fimbrillin forms fimbrial structure and a minor subunit called adhesin localizes at the tip of fimbriae and recognizes host receptor ganglioside. Within this eight gene cluster, fanE and fanF have not yet been sequenced. In this study, fanE and fanF genes were sequenced by analyzing several DNA fragments produced by endonuclease or exonuclease digestion. The fanE gene encoded 227 amino acids containing 20 amino acids of signal peptide starting from GTG (valine) and showed a homology to fanA-fanB. The fanF gene encoded 271 amino acids containing 20 amino acids of signal peptide starting from ATG (methionine) and showed homologies to the fanD gene, fimbrillin gene of F41, adhesin gene of P fimbriae (papG) and adhesin gene of Type 1 fimbriae (fimH). E and F subunits had fifteen and fourteen hydrophobic domains, respectively, which periodically appeared possibly forming a hydrophobic region.  相似文献   

Comparison of DNA-spot hybridization, cell culture and direct immunofluorescence staining for the diagnosis of avian chlamydiae   总被引：1，自引：0，他引：1  

P Timms  F W Eaves  B J Rodwell  M F Lavin 《Veterinary microbiology》1988,18(1):15-25

DNA-spot hybridization, cell culture and direct immunofluorescence staining were compared for the detection of avian Chlamydia psittaci strains in cell culture dilutions and in routine samples submitted for diagnosis. With dilutions of infected cell culture material, growth in BGM cells was by far the most sensitive technique, detecting 0.01 infected cells (20 elementary bodies) ml-1. DNA-spot hybridization and direct immunofluorescence staining were of approximately equal sensitivity, both detecting 16 infected cells (3.2 x 10(4) elementary bodies) per ml-1. When 27 avian liver and spleen samples were assayed, all 3 tests performed similarly (13 positive and 12 negative by all 3 tests). This suggests that in most avian samples presented for diagnosis, sufficient numbers of chlamydiae are present to allow any of the test to the be used. Thus, the direct immunofluorescence staining method is currently the test of choice for routine diagnosis since it is available in kit form, is relatively simple and quick to perform, and like DNA-spot hybridization, detects non-viable as well as viable organisms. However, if low levels of chlamydiae are to be effectively detected, such as in carrier birds or birds with recently acquired infections, then cell culture should be used.  相似文献   

ANTIRABIC IMMUNIZATION WITH CULTURE VIRUS RENDERED AVIRULENT BY ULTRA-VIOLET LIGHT     

Hodes HL  Lavin GI  Webster LT 《Science (New York, N.Y.)》1937,86(2237):447-448

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REDUCTION OF OXYLUCIFERIN BY ATOMIC HYDROGEN     

Harvey EN  Lavin GI 《Science (New York, N.Y.)》1931,74(1910):150-151

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Comment on "Single Crystals of Single-Walled Carbon Nanotubes Formed by Self-Assembly"     

Chisholm MF  Wang Y  Lupini AR  Eres G  Puretzky AA  Brinson B  Melechko AV  Geohegan DB  Cui H  Johnson MP  Pennycook SJ  Lowndes DH  Arepalli S  Kittrell C  Sivaram S  Kim M  Lavin G  Kono J  Hauge R  Smalley RE 《Science (New York, N.Y.)》2003,300(5623):1236; author reply 1236

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ATM activation by oxidative stress   总被引：2，自引：0，他引：2  

Guo Z  Kozlov S  Lavin MF  Person MD  Paull TT 《Science (New York, N.Y.)》2010,330(6003):517-521

The ataxia-telangiectasia mutated (ATM) protein kinase is activated by DNA double-strand breaks (DSBs) through the Mre11-Rad50-Nbs1 (MRN) DNA repair complex and orchestrates signaling cascades that initiate the DNA damage response. Cells lacking ATM are also hypersensitive to insults other than DSBs, particularly oxidative stress. We show that oxidation of ATM directly induces ATM activation in the absence of DNA DSBs and the MRN complex. The oxidized form of ATM is a disulfide-cross-linked dimer, and mutation of a critical cysteine residue involved in disulfide bond formation specifically blocked activation through the oxidation pathway. Identification of this pathway explains observations of ATM activation under conditions of oxidative stress and shows that ATM is an important sensor of reactive oxygen species in human cells.  相似文献