Influence of Plant Growth Promoting Rhizobacteria,Compost, and Biochar of Azolla on Rosemary (Rosmarinus Officinalis L.) Growth and Some Soil Quality Indicators in a Calcareous Soil     

Leila Sadegh Kasmaei  Mehdi Zarei  Abdolmajid Ronaghi  Reza Ghasemi  Mohammad Jamal Saharkhiz 《Communications in Soil Science and Plant Analysis》2019,50(2):119-131

In order to study the effect of plant growth promoting rhizobacteria (PGPR), Azolla compost and Azolla biochar on some soil quality indicators and rosemary growth, a greenhouse experiment was conducted in a completely randomized design with six replications. Treatments consisted of T₁ (control), T₂ (1% Azolla compost), T₃ (1% Azolla biochar), T₄ (PGPR (P. fluorescens), T₅ (1% compost + PGPR) and T₆ (1% biochar + PGPR). Rosemary growth parameters and nutrients concentration increased in all treatments compared to control. Treatments increased soil nutrient concentrations, soil microbial respiration (SMR) and microbial biomass C (MBC) but decreased soil metabolic quotient (qCO₂) compared to control treatment. A significant enhancement in rosemary growth occurred due to the improved soil quality as a result of organic fertilizers application, particularly by co-application of P. fluorescens and compost or biochar of Azolla.  相似文献   

Preventive factors related to brucellosis among rural population using the PRECEDE model: an application of path analysis     

Jahangiry  Leila  Khazaee-Pool  Maryam  Mahdavi  Babak  Ponnet  Koen  Sarbakhsh  Parvin 《Tropical animal health and production》2019,51(2):419-428

Tropical Animal Health and Production - The purpose of this model-based study was to identify behavioral and environmental prevention factors for brucellosis and to determine the causal linkage...  相似文献   

An atypical Bacillus anthracis infection in a bull—A potential occupational health hazard     

Mari Friman  Leila Kakko  Camelia Constantin  Heli Simojoki  Maria A. Andersson  Szabolcs Nagy  Heidi Salonen  Magnus Andersson 《Reproduction in domestic animals》2019,54(9):1279-1283

Bacillus anthracis infecting cattle is usually identified based on the typical symptom: sudden death. Bacillus anthracis causing atypical symptoms may remain undiagnosed and represent a potential occupational health hazard for, that is veterinarians and producers, butchers and tanners. In the year 2004, one case of sudden death in a dairy farm in southern Finland was diagnosed as bovine anthrax. Four years later 2008, an atypical case of anthrax was diagnosed in the same holding. The bull was taken to the Production Animal Hospital of the Faculty of Veterinary Medicine, University of Helsinki because of fever, loss of appetite and a symmetrically swollen scrotal sac. Penicillin treatment cured the fever but not the swollen scrotum. Before the intended therapeutic castration, a punctuate consisting of 10 ml fluid collected into a syringe from the scrotal sac was cultivated on blood agar at 37°C. After 24 hr, an almost pure culture of a completely non‐hemolytic Bacillus cereus‐like bacteria was obtained. The strain was identified as B. anthracis using Ba‐specific primers by the Finnish Food Safety Authority (RUOKAVIRASTO). After the diagnosis, the bull was euthanized and destroyed, the personnel were treated with prophylactic antibiotics and the clinic was disinfected. In this particular case, treatment with water, Virkon S and lime seemed to be effective to eliminate endospores and vegetative cells since no relapses of anthrax have occurred in 10 years. This case is the last reported anthrax case in Finland.  相似文献   

Papillary architecture of the Leopardus pardalis tongue     

Elisa Carla Barra Freire  Lityane Gabriela da Silva Moreira  Elane Guerreiro Giese  rika Branco  Leila Menezes da Silva  Ana Rita de Lima 《Anatomia, histologia, embryologia》2019,48(5):421-428

The ocelot (Leopardus pardalis) is a Felidae of wide geographical distribution and food flexibility; therefore, it is essential to understand the morphology of the species. Thus, we aimed to describe its lingual morphology in order to gain information regarding the anatomy of this carnivore's digestive system. The tongues ??were removed for ex situ macroscopic and morphometric analyses, as well as for light microscopy and scanning electron microscopy, of fragments of the different lingual regions. The tongue of L. pardalis had an elongated form that was subdivided into the apex, body and root, in which four papillary types were observed: filiform, fungiform, circumvallate and conical. It presented with a stratified, keratinized squamous epithelium, followed by loose and dense connective tissues, as well as a skeletal striated musculature that comprised most of the organ. In addition, in scanning electron microscopy the filiform papillae showed a complex with multiple layers of keratin with triangular shape projected caudally in oral cavity. The fungiform papillae were distributed among the filiform and showed a rounded shape with some gustatory pores, and are keratinized but in a lower intensity if compared with filiform. The vallate papillae, located in lingual root, showed an oval format, had a deep groove surrounded the papillae and some gustatory pores. The conical papillae are located in lingual root and are similar to the filiform. The tongue of L. pardalis resembles other carnivorous species, mainly among felids. However, it differed in relation to the quantity of vallate papillae and the absence of foliate papillae.  相似文献   

Chemical,physical and micromorphological properties of termite mounds and adjacent soils along a toposequence in Zona da Mata,Minas Gerais State,Brazil     

Tathiane Santi Sarcinelli  Carlos Ernesto G.R. Schaefer  Leila de Souza Lynch  Helga Dias Arato  João Herbert M. Viana  Manoel Ricardo de Albuquerque Filho  Teresa Telles Gonçalves 《CATENA》2009

Little is known about the effects of neotropical mound-building termites in soil chemical and physical properties. The influence of soil termite activity on soil characteristics was studied by assessing chemical, physical and micromorphological properties of a toposequence of Latosols (Oxisols). Soil samples were collected from the walls and inner parts of termite mounds and also from adjacent soil. A high diversity of termite genera was found in the mounds along the toposequence, together with the inquiline termites and other soil-dwelling arthropods. Chemical analyses showed that pH and the contents of organic C and N, P, Ca and Mg were significantly higher in termite mounds compared with adjacent areas, with an inverse trend for Al content. Significant differences in pH and exchangeable Al were observed between soil and mound across the slopes. The mound density across the landscape was higher at the upper slope segment, followed by the hill top, middle slope and lower slope segments. Considering a lifespan of 30 years and dimensions of termite mounds found in the toposequence we conclude that the textural and chemical uniformity of Latosols may be increased, following the pedobiological turnover during mound building, with local rates varying from 2.1 to 7.5 m³ ha^− 1.  相似文献   

Characterization of phytoplasmas related to peanut witches’-broom and stolbur groups associated with alfalfa diseases in Iran     

Hosseini  Somayeh  Bahar  Masoud  Zirak  Leila 《植物病害和植物保护杂志》2013,120(2):70-76

Journal of Plant Diseases and Protection - In the central and south eastern provinces of Iran, alfalfa plants showing phytoplasma diseases-like symptoms categorized into three major types, namely...  相似文献   

Woodchuck Hepatitis Virus Post-Transcriptional Regulation Element (WPRE) Promotes Anti-CD19 BiTE Expression in Expi293 Cells     

Reza Moazzami  Hasan Mirzahoseini  Leila Nematollahi  Farzaneh Barkhordari  Mozhgan Raigani  Fatemeh Hajari Taheri  Fereidoun Mahboudi  Fatemeh Davami 《Iranian Biomedical Journal》2021,25(4):275

Background:Bispecific antibodies represent an important class of mAbs, with great therapeutic potentials due to their ability to target simultaneously two distinct epitopes. The generation of functional bispecific antibodies with the highest possible yields is particularly critical for the production of these compounds on industrial scales. Anti- CD3 × CD19 bsAb is a bispecific T-cell engager (BiTE) currently used for treating ALL. Herein, we have tried to optimize the expression level of this antibody in mammalian hosts. Methods:WPRE sequence was incorporated at the 3’ end of the expression cassette. This modification resulted in a notable about two-fold increase in the expression of the bsAb in the Expi293 cell line. Results & Conclusion:Follow-up flow cytometry analysis demonstrated the binding properties of the produced antibody at acceptable levels, and in vitro bioactivity assays showed that this product is potent enough for targeting and destroying CD19-positive cells. Our findings show that WPRE enhances the expression of this type of bispecific mAbs in HEK-293 family cell lines. This approach can be used in biopharma industry for the mass production of anti-CD3 × CD19 bispecific antibody. Key Words: Acute lymphoblastic leukemia, Bispecific antibodies, Monoclonal antibody  相似文献   

A report of mycobacteriosis caused by Mycobacterium marinum in bullfrogs (Rana catesbeiana)     

Ferreira R  Fonseca Lde S  Afonso AM  da Silva MG  Saad MH  Lilenbaum W 《Veterinary journal (London, England : 1997)》2006,171(1):177-180

The occurrence of mycobacteriosis caused by Mycobacterium marinum in a commercial breeding farm of bullfrogs (Rana catesbeiana) in Rio de Janeiro, Brazil is described. Ten animals presented skin lesions on the head and extremities. These and 38 other asymptomatic adult animals from various tanks were killed and at necropsy disseminated granulomatous lesions were observed in the 10 clinically affected animals and in 16 (42.1%) of the asymptomatic frogs. Acid-fast bacilli were observed in all smears of the 10 symptomatic frogs and in all but one from the 16 asymptomatic animals with visceral lesions. Ten samples from the 25 positive animals were randomly selected for culture which yielded four isolates of fast-growing (<7 days) mycobacteria. Those purified isolates were characterised by biochemical traditional means as M. marinum. Identification of the strains was confirmed using reverse-phase high-performance liquid chromatography and a polymerase chain reaction (PCR) restriction enzyme analysis assay. It is suggested that M. marinum is an important agent of granulomatous disease in bullfrogs and that infected animals, even when asymptomatic, could act as reservoirs spreading the disease and contaminating other frogs in the farm.  相似文献   

What is your diagnosis? Avulsion of the origin of the gastrocnemius muscle     

Ting D  Petersen SW  Mazzaferro EM  Worth LT  Petersen SW;American College of Veterinary Radiology 《Journal of the American Veterinary Medical Association》2006,228(10):1497-1498

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Effects of Microalgae and Alfalfa Meal on Population Growth and Production of a Freshwater Rotifer,Euchlanis dilatata (Rotifera: Mongononta)     

Omidvar Farhadian  Leila Daghighi  Eisa Ebrahimi Dorche 《Journal of the World Aquaculture Society》2013,44(1):86-95

Population growth and production of the freshwater rotifer, Euchlanis dilatata, were determined after being fed 1 of 12 diets: Scenedesmus quadricauda (S), Chlorella vulgaris (C), baker's yeast (Saccharomyces cerevisiae) (Y), alfalfa meal (Medicago spp.) (A), and eight combinations of S+C, S+Y, C+Y, S+C+Y, S+A, C+A, S+C+A, and Y+A (mixture in the same ratio by equal nitrogen weight). Results showed that both microalgal and alfalfa meal alone and in combination with Y had a significantly higher (P < 0.05) population growth and production compared with Y alone. The mean maximum E. dilatata population density fed on S, S+Y, C, C+Y, S+C, S+C+Y, Y, A, S+A, C+A, S+C+A, and Y+A diets were 65 ± 7.5 (mean ± SE), 124.6 ± 17.2, 115.8 ± 7.9, 41.5 ± 7.9, 131 ± 14.1, 93.5 ± 8.7, 14.5 ± 7.6, 129.5 ± 18.5, 255.0 ± 20.5, 180 ± 30.5, 124.5 ± 25.4, and 61.5 ± 24.5 individuals/mL, respectively. Correspondingly, the mean population growth rates (K) through the 16‐d culture period were 0.29, 0.40, 0.45, 0.27, 0.34, 0.41, 0.26, 0.51, 0.58, 0.59, 0.39, and 0.45/d, respectively. Results indicated that alfalfa meal in combination with microalgae and yeast could be used as primary diets for E. dilatata culture. This may reduce the costs of rotifer production.  相似文献